ABSTRACT
In recent years, macroporous adsorption resin has been widely used in the purification and separation of natural medicine and effective components of Chinese materia medica, the purification of compound Chinese medicine preparations, the removal of harmful impurities due to the advantages of stable physical and chemical properties, high selectivity, strong adsorption capacity and easy elution, recyclable use and regeneration treatment, economic and environmental protection, convenient preparation molding and so on. By summarizing the preparation, properties, classification and working principle of macroporous adsorption resin, the influencing factors and purification process of Chinese materia medica components were reviewed. The purpose of this paper is to explore the important factors affecting the purification of Chinese materia medica components by macroporous adsorption resin, and provide reference for improving the purification effect of Chinese materia medica components.
ABSTRACT
This study investigated an efficient and recyclable approach for purification of crude pumpkin oligosaccharide (POS) by macroporous resins. Five resins with different physical and chemical properties were tested for decoloration of POS. In virtue of its higher decoloration ratio (92.6%) and POS recovery ratio (81.3%), the macroporous resin DM28 was considered to a better selection. Depending on the changes of molecular weight, part of the monosaccharides in crude POS were removed simultaneously after decoloration by DM28. Operating conditions were also determined by the dynamic breakthrough and desorption curves. Moreover, UV/vis spectroscopy and Fourier transform infrared results revealed that most of the colored impurities and proteins can be removed, but the characteristic groups of the POS exhibited no significant difference. Compared with traditional methods, DM28 resin is superior in decoloration efficiency, pigment recovery and oligosaccharide recovery. This research contributes to further exploration on the structure and function of POS.