ABSTRACT
OBJECTIVE: To study whether the traditional Chinese medicine Suifukang can inhibit the expression of chondroitin sulfate proteoglycans(CSPGs) in rats with middle cerebral artery occlusion. METHODS: SD rats were selected and randomized into six groups: normal group, control group, methylprednisolone treatment group, and Suifukang high dose, middle dose and low dose treatment groups. Serum pharmacological method was used to prepare serum containing different concentrations of Suifukang. And glial scar model established in vitro was treated with the drug-containg serum. The expression of CSPGs was detected by immunohistology method. RESULTS: The expression of CSPGs in the injured brain and glial scar in vitro were significantly inhibited by Suifukang (P < 0.01). CONCLUSION: Suifukang can inhibit the expression of CSPGs. This function may be one of the mechanisms that Suifukang promotes the regeneration of axon and the functional recovery after ischemical injury of brain.
ABSTRACT
OBJECTIVE: To study the effect of Chinese herb Suifukang on the expression of Nogo protein-A in the brain of MCAO rats and explore the action mechanism of its promoting neurogenesis. METHODS: SD rats were selected and randomized into six groups: normal group, control group, treatment group of methylprednisolone, treatment group of Chinese herb Suifukang in large-does, middle-does and small-does. The models of MCAO rats were successfully established by the method of Koizumi. After the surgery, the rats of drug group had been taken orally Suifukang by 10 g · kg-1 crude drug one time daily, and the rest groups had been taken orally equivalence saline once a day. The brain of rats were investigated and the expression of NoGo-A was detected by immunohistology method and RT-PCR method. RESULTS: The expression of NoGo-A in the injured brain was apparently inhibited by Suifukang (P < 0.01). CONCLUSION: Suifukang can inhibit the expression of NoGo-A. So this function may be one of the mechanisms that Suifukang can promote the functional recovery after brain injury. Copyright 2013 by the Chinese Pharmaceutical Association.