ABSTRACT
INTRODUCTION: This report describes changes in blood and urine concentrations of glyphosate potassium over time and their correlations with clinical symptoms in a patient with acute glyphosate potassium poisoning. CASE REPORT: A 67-year-old man visited the emergency center after ingesting 250â¯mL of a glyphosate potassium-based herbicide 5â¯h before. He was alert but presented with nausea, vomiting, and bradyarrhythmia with atrial fibrillation (tall T waves). Laboratory findings revealed a serum potassium level of 6.52â¯mEq/L. After treatment with an injection of calcium gluconate, insulin with glucose, bicarbonate, and an enema with polystyrene sulfonate, the patient's serum potassium level normalized and the bradyarrhythmia converted to a normal sinus rhythm. During admission, the blood and urine concentration of glyphosate and urine aminomethylphosphonic acid (AMPA, a glyphosate metabolite) was measured at regular time intervals. The patient's glyphosate blood concentration on admission was 11.48â¯mg/L, and it had decreased rapidly by 16â¯h and maintained about 1mgl/L by 70â¯h after admission. Urine glyphosate and AMPA levels had also decreased rapidly by 6â¯h after admission. DISCUSSION: Glyphosate potassium poisoning causes hyperkalemia. Blood concentrations of glyphosate were decreased rapidly by 16â¯h after admission, and urine concentrations were also decreased by 6â¯h after admission.
Subject(s)
Glycine/analogs & derivatives , Herbicides/blood , Herbicides/poisoning , Hyperkalemia/chemically induced , Aged , Arrhythmias, Cardiac/chemically induced , Glycine/blood , Glycine/poisoning , Glycine/urine , Herbicides/urine , Humans , Hyperkalemia/blood , Hyperkalemia/drug therapy , Male , Nausea/chemically induced , Potassium/blood , Suicide, Attempted , Treatment Outcome , Vomiting/chemically induced , GlyphosateABSTRACT
An UPLC-HR-MS metabolomics approach was used to study the effects of a 49-days oral supplementation with Polygonum cuspidatum extract in healthy rats. Multivariate analysis allowed to observe significant differences in the excretion of several markers between treated animals and control group. Among the others, the amounts of N-methyl-2-pyridone-5-carboxamide (2PY) and phenylacetylglycine (PAG) were reduced in the treated group compared to control. These compounds have been previously considered as markers of aging. Furthermore, the excretion of 3-hydroxysebacic acid and 4,6-dihydroxyquinoline was also changed following supplementation, although not significantly. Despite the relatively short time of treatment (7â¯weeks), the significant changes in the urinary levels of aging markers observed at day 49 suggests a potential role of this type of studies as a new approach in the evaluation of the anti-aging effects of plant extracts.
Subject(s)
Aging/drug effects , Fallopia japonica/chemistry , Plant Extracts/pharmacology , Stilbenes/pharmacology , Animals , Biomarkers/urine , Chromatography, High Pressure Liquid , Female , Glycine/analogs & derivatives , Glycine/urine , Male , Mass Spectrometry , Metabolomics , Pyridones/urine , Rats , Rats, Sprague-Dawley , ResveratrolABSTRACT
The biosynthesis of creatine (Cr) is closely related to the bioavailability of guanidinoacetate (GAA). The lack of one or the other may compromise their role in the energy transport and cell signaling. A reliable estimate of their levels in biological samples is imperative since they are important markers of many metabolic disorders. Therefore, a new LC-MS/MS method for simultaneous determination and quantification of GAA and Cr by multiple reaction monitoring (MRM) mode was developed based on the hydrophilic interaction chromatography (HILIC) and response surface methodology (RSM) for the optimization of chromatographic parameters. The optimized parameters ensured good separation of these similar, very polar molecules (chromatographic resolutionâ¯>â¯1.5) without prior derivatization step in a short analysis run (6â¯min). The developed method was validated to ensure accurate (R, 75.1-101.6%), precise (RSDâ¯<â¯20%) and low quantification (LOQ of 0.025⯵gâ¯mL-1 for GAA and 0.006⯵gâ¯mL-1 for Cr) of the tested analytes and the use of matrix-matched calibration eliminated variable effects of complex matrices such as human plasma and urine. Therefore, this method can be implemented in medical laboratories as a tool for the diagnostics of creatine deficiencies and monitoring of guanidinoacetate and creatine supplementation regimes in biological samples.
Subject(s)
Chromatography, Liquid/methods , Creatine/analysis , Glycine/analogs & derivatives , Hydrophobic and Hydrophilic Interactions , Tandem Mass Spectrometry/methods , Calibration , Creatine/blood , Creatine/urine , Glycine/analysis , Glycine/blood , Glycine/urine , Humans , Limit of Detection , Reproducibility of Results , Time FactorsABSTRACT
In two feeding experiments the retention of supplemental guanidine acetic acid (GAA) in broilers was investigated. In both experiments, the same three treatments were used; the basal feed was supplemented with 0, 0.6, or 6.0 g GAA per kg of feed. While in a growth study (experiment 1) day-old, male Ross 308 broilers were fed diets for 35 days, these diets were fed for only 8 days to fistulated broilers 34 days of age in a balance study (experiment 2). Feeding 0.6 g/kg GAA did not improve growth performance whereas 6.0 g/kg GAA resulted in a reduction of feed consumption and consequently of weight gain (P ≤ 0.05). Feed conversion was not affected and was 1.48 to 1.49 in all treatments. Increasing levels of dietary GAA gradually increased the creatine concentration in breast muscle and liver tissues (P ≤ 0.05) indicating a transformation and retention of dietary GAA as creatine. In experiment 2 the non-supplemented basal diet allowed us to determine the endogenous GAA, creatine, and creatinine excretions. Accordingly, only small amounts of these metabolites were recovered in feces while they were much higher in urine. Increasing dietary GAA intake increased fecal and renal GAA, creatine, and creatinine excretion and was significant (P ≤ 0.05) at 6.0 g/kg dietary GAA compared to no or 0.6 g/kg GAA supplementation. The mean true fecal digestibility of GAA (99%) was unaffected by the level of supplemental GAA. Considering renal GAA excretions, true availability of supplemental GAA was reduced with increasing dose (83% vs. 71%; P ≤ 0.05). Taking into account creatine and creatinine excretions above those of the basal diet, as they are a consequence of increasing dietary supply, true availability of supplemental GAA shrank from 76% (0.6 g/kg GAA) to 46% (6.0 g/kg GAA; P ≤ 0.05). Changes in blood creatine and creatinine levels reflected the changes observed in the liver and muscle tissues and may suggest increased transport to excretion organs. Data from these experiment were used to estimate the creatine requirement.
Subject(s)
Animal Nutritional Physiological Phenomena , Chickens/physiology , Creatine/metabolism , Dietary Supplements , Glycine/analogs & derivatives , Animal Feed/analysis , Animals , Chickens/growth & development , Creatine/urine , Diet/veterinary , Digestion , Feces/chemistry , Glycine/metabolism , Glycine/urine , MaleABSTRACT
Specific and sensitive food biomarkers are necessary to support dietary intake assessment and link nutritional habits to potential impact on human health. A multistep nutritional intervention study was conducted to suggest novel biomarkers for coffee consumption. (1)H NMR metabolic profiling combined with multivariate data analysis resolved 2-furoylglycine (2-FG) as a novel putative biomarker for coffee consumption. We relatively quantified 2-FG in the urine of coffee drinkers and investigated its origin, metabolism, and excretion kinetics. When searching for its potential precursors, we found different furan derivatives in coffee products, which are known to get metabolized to 2-FG. Maximal urinary excretion of 2-FG occurred 2 h after consumption (p = 0.0002) and returned to baseline after 24 h (p = 0.74). The biomarker was not excreted after consumption of coffee substitutes such as tea and chicory coffee and might therefore be a promising acute biomarker for the detection of coffee consumption in human urine.
Subject(s)
Coffee/metabolism , Glycine/analogs & derivatives , Adult , Biomarkers , Feeding Behavior , Female , Glycine/metabolism , Glycine/urine , Humans , Male , Metabolomics , Middle Aged , Young AdultABSTRACT
BACKGROUND: Characterization of the variations in the metabolomic profiles of elderly people is a necessary step to understand changes associated with aging. This study assessed whether diets with different fat quality and supplementation with coenzyme Q10 (CoQ) affect the metabolomic profile in urine analyzed by proton nuclear magnetic resonance spectroscopy from elderly people. METHODS: Ten participants received, in a cross-over design, four isocaloric diets for 4-week periods each: Mediterranean diet supplemented with CoQ (Med + CoQ diet); Mediterranean diet; Western diet rich in saturated fat diet; low-fat, high-carbohydrate diet enriched in n-3 polyunsaturated fat. RESULTS: Multivariate analysis showed differences between diets when comparing Med + CoQ diet and saturated fat diet, with greater hippurate urine levels after Med + CoQ diet and higher phenylacetylglycine levels after saturated fat diet in women. Following consumption of Med + CoQ, hippurate excretion was positively correlated with CoQ and ß-carotene plasma levels and inversely related to Nrf2, thioredoxin, superoxide dismutase 1, and gp91(phox) subunit of NADPH oxidase gene expression. After saturated fat diet consumption, phenylacetylglycine excretion was inversely related to CoQ plasma level and positively correlated with isoprostanes urinary level. CONCLUSIONS: The association between hippurate excretion and antioxidant biomarkers along with the relationship between phenylacetylglycine excretion and oxidant biomarkers suggests that the long-term consumption of a Med + CoQ diet could be beneficial for healthy aging and a promising challenge in the prevention of processes related to chronic oxidative stress, such as cardiovascular and neurodegenerative disease.
Subject(s)
Aging/metabolism , Diet, Mediterranean , Ubiquinone/analogs & derivatives , Aged , Aging/genetics , Antioxidants/administration & dosage , Biomarkers/metabolism , Cross-Over Studies , Dietary Fats/administration & dosage , Dietary Supplements , Female , Gene Expression , Glycine/analogs & derivatives , Glycine/urine , Hippurates/urine , Humans , Male , Metabolomics , Oxidative Stress , Ubiquinone/administration & dosage , beta Carotene/bloodABSTRACT
BACKGROUND: Beta-ketothiolase deficiency is a rare inborn errors of metabolism (IEM) affecting the catabolism of isoleucine, characterized by severe ketoacidosis in children of 6 to 24months old. A prompt diagnosis is of paramount importance as the metabolic decompensation can be effectively reverted by glucose infusion and health outcomes are improved on a protein-restricted diet. Currently, majority of the laboratory diagnosis were made based on mass-spectrometry and molecular genetics while little is mentioned on the advancement of nuclear magnetic resonance (NMR) spectroscopy for the diagnosis of this condition. CASE: We report a case of beta-ketothiolase deficiency in a 1-y-old Chinese boy who presented with repeated vomiting, impaired consciousness and severe ketoacidosis. NMR urinalysis detected excessive amount of butanone (a disease specific marker of beta-ketothiolase deficiency), tiglylglycine, (intermediate of isoleucine catabolism) and ketones. Diagnosis of beta-ketothiolase deficiency was further established by molecular genetic studies of ACAT1 gene of the proband. CONCLUSIONS: This case illustrated that NMR-based urinalysis is complementary to organic acid analysis for diagnosis of beta-ketothiolase deficiency. The operation of NMR is simple and fast; sample preparation is a two-step procedure while the NMR acquisition is automatic and usually takes <15min. We envisage that NMR analysis will become more available in clinical laboratories and will play an important role in acute pediatric care.
Subject(s)
Acetyl-CoA C-Acyltransferase/deficiency , Amino Acid Metabolism, Inborn Errors/diagnosis , Amino Acid Metabolism, Inborn Errors/urine , Biomarkers/urine , Magnetic Resonance Imaging/methods , Urinalysis/methods , Acetyl-CoA C-Acyltransferase/urine , Butanones/urine , Gas Chromatography-Mass Spectrometry , Glycine/analogs & derivatives , Glycine/urine , Humans , Infant , Ketones/urine , MaleABSTRACT
In previous nephrotoxicity metabonomic studies, several potential biomarkers were found and evaluated. To investigate the relationship between the nephrotoxicity biomarkers and the therapeutic role of Radix Glycyrrhizae extract on Semen Strychni-induced renal failure, 12 typical biomarkers are selected and a simple LC-MS method has been developed and validated. Citric acid, guanidinosuccinic acid, taurine, guanidinoacetic acid, uric acid, creatinine, hippuric acid, xanthurenic acid, kynurenic acid, 3-indoxyl sulfate, indole-3-acetic acid, and phenaceturic acid were separated by a Phenomenex Luna C18 column and a methanol/water (5 mM ammonium acetate) gradient program with a runtime of 20 min. The prepared calibration curves showed good linearity with regression coefficients all above 0.9913. The absolute recoveries of analytes from serum and urine were all more than 70.4%. With the developed method, analytes were successfully determined in serum and urine samples within 52 days. Results showed that guanidinosuccinic acid, guanidinoacetic acid, 3-indoxyl sulfate, and indole-3-acetic acid (only in urine) were more sensitive than the conventional renal function markers in evaluating the therapeutic role of Radix Glycyrrhizae extract on Semen Strychni-induced renal failure. The method could be further used in predicting and monitoring renal failure cause by other reasons in the following researches.
Subject(s)
Drugs, Chinese Herbal/therapeutic use , Renal Insufficiency/drug therapy , Animals , Biomarkers/blood , Biomarkers/urine , Chromatography, Liquid , Citric Acid/blood , Citric Acid/urine , Creatinine/blood , Creatinine/urine , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/toxicity , Glycine/analogs & derivatives , Glycine/blood , Glycine/urine , Guanidines/blood , Guanidines/urine , Hippurates/blood , Hippurates/urine , Indican/blood , Indican/urine , Indoleacetic Acids/blood , Indoleacetic Acids/urine , Kynurenic Acid/blood , Kynurenic Acid/urine , Male , Mass Spectrometry , Medicine, Chinese Traditional , Molecular Structure , Plant Extracts/chemistry , Rats , Rats, Sprague-Dawley , Renal Insufficiency/chemically induced , Succinates/blood , Succinates/urine , Taurine/blood , Taurine/urine , Uric Acid/blood , Uric Acid/urine , Xanthurenates/blood , Xanthurenates/urineABSTRACT
PURPOSE: Guanidinoacetic acid (GAA) is an intermediate in the biosynthesis of creatine (Cr), yet its use in human nutrition is limited due to a lack of a clear understanding of its' dose-response effect. Thus, the purpose of this study was to investigate the effect of three different dosages of GAA (1.2, 2.4 and 4.8 g/day) administered for 6 weeks on serum and urinary variables related to GAA metabolism. METHODS: Forty-eight healthy volunteers participated in the randomized, placebo-controlled, double-blind, repeated-measure study. At baseline, after 1, 2, 4 and 6 weeks, participants provided both fasting blood samples and 24-h urine. RESULTS: GAA intervention significantly increased serum and urinary GAA, Cr and creatinine as compared to placebo (P < 0.05). Differences were found for serum GAA and Cr responses between the three GAA dosages, with high-dose GAA resulting in a greater increase (P < 0.05) in the plasma concentration of both variables as compared to other GAA dosages. In GAA groups, fasting plasma total homocysteine (T-Hcy) increased by 3.5 µmol/L on average at post-administration, yet no dose-response differences were found between trials. Serum B vitamins were not affected by either placebo or GAA intervention (P > 0.05). CONCLUSION: Results indicate that low-to-high dosages of exogenous GAA can increase serum concentrations of Cr and T-Hcy while not depleting the B vitamins pool available for remethylation of homocysteine. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov, identification number NCT01133899.
Subject(s)
Creatine/blood , Glycine/analogs & derivatives , Homocysteine/blood , Vitamin B Complex/blood , Administration, Oral , Creatine/urine , Creatinine/urine , Dietary Supplements , Dose-Response Relationship, Drug , Double-Blind Method , Female , Follow-Up Studies , Glycine/administration & dosage , Glycine/urine , Humans , Male , Young AdultABSTRACT
There is a wealth of experimental information and some clinical evidence available in the literature suggesting that taurine exerts preventive effects on cardiovascular diseases. In particular, taurine has been shown to reduce blood pressure in not only hypertensive patients but also in a number of hypertensive rodent models such as spontaneously hypertensive rats (SHR). However, the molecular basis of the efficacy and toxicity of the compound has not been fully characterized. We have investigated the effects of taurine supplementation to urinary low-molecular-weight endogenous metabolites in SHR using a (1)H NMR-based urinary metabonomic approach. The SHR were chronically treated with 3% taurine in drinking water from four to 14 weeks of age, and 24-h urine samples were analyzed using (1)H NMR spectroscopy. Metabolic information was extracted from the NMR data by principal components analysis as well as visual inspection. Consequently, the metabolite profile started to change with considerable interindividual variation from six weeks of age. The extent of change became increasingly remarkable with the duration of treatment, with the concurrent observation of the hypotensive effect. The metabolic changes included a decreased urinary output of tricarboxylic acid cycle intermediates (citrate, α-ketoglutarate, and succinate) and an increased output of phenylacetylglycine and p-cresol sulfate. The results suggest that chronic taurine supplementation to the SHR resulted in an acceleration of metabolic acidosis with perturbation in the tricarboxylic acid cycle and the modulation of the intestinal microbial metabolism.
Subject(s)
Magnetic Resonance Spectroscopy/methods , Metabolomics/methods , Taurine/pharmacology , Acidosis/chemically induced , Animals , Blood Pressure/drug effects , Citric Acid Cycle/drug effects , Cresols/urine , Dietary Supplements , Glycine/analogs & derivatives , Glycine/urine , Male , Rats , Rats, Inbred SHR , Sulfuric Acid Esters/urine , UrinalysisABSTRACT
We adopted an ultra performance liquid chromatography coupled with quadrupole time-of-fight mass spectrometry (UPLC Q-TOF/MS) metabonomics approach to study metabonomic features of rats induced by orally administered Pinellia ternata (Thunb.) Berit. (BX). The integrated urinary MS data were analyzed via principal component analysis (PCA) and partial least squares-discriminant analysis (OPLS-DA) to identify the differential metabolites. Ten potential biomarkers were identified within complex sample matrix of urine. The identified biomarkers indicated the perturbations of tryptophan, phenylacetylglycine and pantothenic acid metabolism in BX-induced rats. The biomarkers that were found to be changed with the passage of time were explained tentatively based on previous study.
Subject(s)
Medicine, Chinese Traditional , Metabolomics/methods , Pinellia/toxicity , Animals , Chromatography, High Pressure Liquid/methods , Glycine/analogs & derivatives , Glycine/urine , Male , Mass Spectrometry , Pantothenic Acid/urine , Principal Component Analysis , Rats , Rats, Sprague-Dawley , Tryptophan/urine , UrinalysisABSTRACT
We report two sisters, aged 11 and 6years, with AGAT deficiency syndrome (OMIM 612718) which is the least common creatine deficiency syndrome. They were born full-term to consanguineous parents and had moderate developmental delay. Examination showed an important language delay, a progressive proximal muscular weakness in the lower limbs with Gowers sign and myopathic electromyography. Investigations revealed undetectable guanidinoacetate and low level of creatine in plasma and urine, characteristic findings of AGAT deficiency syndrome. Brain magnetic resonance spectroscopy showed a markedly reduced level of creatine. Guanidinoacetate methyltransferase (GATM) gene sequencing revealed a homozygous missense mutation in exon 4:c.608A>C, (p.Tyr203Ser). Thirteen months after beginning the treatment with oral creatine monohydrate 200mg/kg/day, then 400mg/kg/day, there was a dramatic improvement in muscle strength with Gowers sign disappearance in both patients, and a mild improvement in language and cognitive functions. AGAT deficiency syndrome should be considered in all patients with language retardation and cognitive impairment associated to a myopathy of unknown etiology such that early diagnosis must lead to creatine supplementation to cure the myopathy and improve language and cognitive function.
Subject(s)
Amidinotransferases/deficiency , Amino Acid Metabolism, Inborn Errors , Intellectual Disability , Speech Disorders , Amidinotransferases/genetics , Amidinotransferases/metabolism , Amino Acid Metabolism, Inborn Errors/genetics , Amino Acid Metabolism, Inborn Errors/metabolism , Amino Acid Metabolism, Inborn Errors/physiopathology , Brain/metabolism , Brain/pathology , Child , Creatine/blood , Creatine/urine , Developmental Disabilities/genetics , Developmental Disabilities/metabolism , Developmental Disabilities/physiopathology , Female , Glycine/analogs & derivatives , Glycine/blood , Glycine/urine , Humans , Intellectual Disability/genetics , Intellectual Disability/metabolism , Intellectual Disability/physiopathology , Magnetic Resonance Spectroscopy , Speech Disorders/genetics , Speech Disorders/metabolism , Speech Disorders/physiopathologyABSTRACT
A rapid, highly sensitive, and selective method was applied in a non-invasive way to investigate the antidepressant action of Xiaoyaosan (XYS) using ultra performance liquid chromatography-mass spectrometry (UPLC-MS) and chemometrics. Many significantly altered metabolites were used to explain the mechanism. Venlafaxine HCl and fluoxetine HCl were used as chemical positive control drugs with a relatively clear mechanism of action to evaluate the efficiency and to predict the mechanism of action of XYS. Urine obtained from rats subjected to chronic unpredictable mild stress (CUMS) was analyzed by UPLC-MS. Distinct changes in the pattern of metabolites in the rat urine after CUMS production and drug intervention were observed using partial least squares-discriminant analysis. The results of behavioral tests and multivariate analysis showed that CUMS was successfully reproduced, and a moderate-dose XYS produced significant therapeutic effects in the rodent model, equivalent to those of the positive control drugs, venlafaxine HCl and fluoxetine HCl. Metabolites with significant changes induced by CUMS were identified, and 17 biomarker candidates for stress and drug intervention were identified. The therapeutic effect of XYS on depression may involve regulation of the dysfunctions of energy metabolism, amino acid metabolism, and gut microflora changes. Metabonomic methods are valuable tools for measuring efficacy and mechanisms of action in the study of traditional Chinese medicines.
Subject(s)
Antidepressive Agents/therapeutic use , Drugs, Chinese Herbal/therapeutic use , Gastrointestinal Tract/microbiology , Metabolic Networks and Pathways/drug effects , Microbiota/drug effects , Phytotherapy , Animals , Antidepressive Agents/urine , Benzoates/urine , Biomarkers/urine , Bridged-Ring Compounds/urine , Catechin/urine , Chalcone/analogs & derivatives , Chalcone/urine , Chromatography, Liquid , Citric Acid/urine , Citric Acid Cycle/drug effects , Coumaric Acids/urine , Creatine Kinase/drug effects , Creatine Kinase/urine , Creatinine/urine , Cyclohexanols/therapeutic use , Drugs, Chinese Herbal/analysis , Flavanones/urine , Fluoxetine/therapeutic use , Gallic Acid/urine , Glucosides/urine , Glycine/analogs & derivatives , Glycine/drug effects , Glycine/urine , Hippurates/urine , Ketoglutaric Acids/urine , Kynurenic Acid/urine , Male , Mass Spectrometry , Metabolomics , Monoterpenes , Plant Extracts/therapeutic use , Rats , Rats, Sprague-Dawley , Stress, Psychological/drug therapy , Tryptophan/drug effects , Tryptophan/urine , Tyrosine/drug effects , Tyrosine/urine , Venlafaxine HydrochlorideABSTRACT
NMR-based metabolite profiling of urine is a fast and reproducible method for detection of numerous metabolites with diverse chemical properties. However, signal overlap in the (1)H NMR profiles of human urine may hamper quantification and identification of metabolites. Therefore, a new method has been developed using automated solid-phase extraction (SPE) combined with NMR metabolite profiling. SPE-NMR of urine resulted in three fractions with complementary and reproducible sub-profiles. The sub-profile from the wash fraction (100 % water) contained polar metabolites; that from the first eluted fraction (10 % methanol-90 % water) semi-polar metabolites; and that from the second eluted fraction (100 % methanol) aromatic metabolites. The method was validated by analysis of urine samples collected from a crossover human nutritional intervention trial in which healthy volunteers consumed capsules containing a polyphenol-rich mixture of red wine and grape juice extract (WGM), the same polyphenol mixture dissolved in a soy drink (WGM_Soy), or a placebo (PLA), over a period of five days. Consumption of WGM clearly increased urinary excretion of 4-hydroxyhippuric acid, hippuric acid, 3-hydroxyphenylacetic acid, homovanillic acid, and 3-(3-hydroxyphenyl)-3-hydroxypropionic acid. However, there was no difference between the excreted amounts of these metabolites after consumption of WGM or WGM_Soy, indicating that the soy drink is a suitable carrier for WGM polyphenols. Interestingly, WGM_Soy induced a significant increase in excretion of cis-aconitate compared with WGM and PLA, suggesting a higher demand on the tricarboxylic acid cycle. In conclusion, SPE-NMR metabolite sub-profiling is a reliable and improved method for quantification and identification of metabolites in urine to discover dietary effects and markers of phytochemical exposure.
Subject(s)
Magnetic Resonance Spectroscopy/standards , Solid Phase Extraction/standards , Urinalysis/methods , Urine/chemistry , Glycine/analogs & derivatives , Glycine/metabolism , Glycine/urine , Hippurates/metabolism , Hippurates/urine , Humans , Male , Middle Aged , Reproducibility of ResultsABSTRACT
BACKGROUND: Guanidinoacetate methyltransferase (GAMT) deficiency is a recently described disorder and few cases have been reported to date. As it is a treatable pathology, we seek to contribute to its better understanding, particularly to further elucidate its biochemical diagnosis for early treatment. METHODS: The patients, two brothers aged 13 years (P1) and 11 years (P2), have been explored for signs and symptoms suggestive of inborn errors of metabolism. The quantification of creatine (Cr), guanidinoacetate (GAA), and GAMT activity was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: The two brothers presented a similar clinical picture: developmental delay, epilepsy, axial hypotonia, spastic tetraparesis, severe mental and language delay, and autistic behaviour. GAA concentrations were markedly increased in plasma and in urine [2796 and 3342 micromol/mmol creatinine (control range: 4 - 220 micromol/mmol creatinine)/14 and 29 micromol/L (control range: 0.35 - 1.8 micromol/L), respectively] while plasma and urine creatine concentrations were at the lower normal range limit. Activity of GAMT in lymphoblasts was extremely reduced (< 0.01 nmol/mg protein/hour) compared to healthy subjects. GAMT activity was found to be intermediary in patients' parents. CONCLUSIONS: It appears that the clinical picture is heterogeneous but should be considered as potential signs of creatine metabolism disorders, however, the biochemical diagnosis is reliable as the enzyme activity is zero in most cases. To date, it is still too early to establish correlations between symptoms and biochemical profile. However, the identification of additional cases of GAMT deficiency should help elucidate such relationships and the progress of patients treated with creatine in conjunction with ornithine supplementation.
Subject(s)
Abnormalities, Multiple/enzymology , Amino Acid Metabolism, Inborn Errors/enzymology , Creatine/metabolism , Guanidinoacetate N-Methyltransferase/deficiency , Siblings , Adolescent , Amino Acid Metabolism, Inborn Errors/genetics , Child , Chromatography, High Pressure Liquid , Consanguinity , Female , Genetic Predisposition to Disease , Glycine/analogs & derivatives , Glycine/blood , Glycine/urine , Guanidinoacetate N-Methyltransferase/genetics , Guanidinoacetate N-Methyltransferase/metabolism , Humans , Lymphocytes/enzymology , Lymphocytes/pathology , Male , Tandem Mass Spectrometry , TunisiaABSTRACT
Metabolic profiling of urine from pesticide-treated rats was investigated by the nuclear magnetic resonance (NMR)-based metabonomic strategy. Twenty-four-hour urine samples of rats were collected after administration with propoxur at doses of 0.85, 1.70, and 8.51 mg/kg, respectively, for 28 consecutive days. Liver tissue was fixed and the histopathological alterations were examined. The results showed that propoxur at high dose induced liver histopathological injury. Metabonomic analysis demonstrated that the levels of creatine and taurine markedly increased together with slight elevation of hippurate, glucose, and amino acids in low- and medium-dose groups. However, concentrations of urinary lactate, acetate, acetone, succinate, citrate, and 2-oxoglutarate increased in high-dose group. All these results suggested that propoxur could inhibit liver function through altering the energy and lipid metabolism. These data also supported the contention that the NMR-based metabonomic approach represents a promising new technology for the development of pesticide toxicity screening and mechanism exploration.
Subject(s)
Insecticides/toxicity , Insecticides/urine , Metabolome/drug effects , Propoxur/toxicity , Propoxur/urine , Animals , Carboxylic Acids/urine , Creatine/urine , Glycine/urine , Insecticides/pharmacokinetics , Liver/drug effects , Liver/pathology , Magnetic Resonance Spectroscopy , Male , Metabolomics , Propoxur/pharmacokinetics , Rats , Rats, Wistar , Taurine/urineABSTRACT
BACKGROUND: Arginine:glycineamidinotransferase (AGAT/GATM) deficiency has been described in 9 patients across 4 families. Here we describe the clinical outcome and response to creatine supplementation in a patient of the second family affected with AGAT deficiency-a 9-year-old girl. PATIENT AND METHODS: Delayed motor milestones were noticed from 4 months of age and at 14 months moderate hypotonia, developmental delay and failure to thrive. Laboratory studies revealed low plasma creatine as well as extremely low levels of guanidinoacetic acid in urine and plasma. Proton magnetic resonance spectroscopy (MRS) of the brain showed absence of creatine. DNA sequence analysis revealed a homozygous mutation (c.484+1G>T) in the AGAT/GATM gene. AGAT activity was not detectable in lymphoblasts and RNA analysis revealed a truncated mRNA (r.289_484del196) that is degraded via Nonsense Mediated Decay. At 16 months, Bayley's Infant Development Scale (BIDS) showed functioning at 43% of chronologic age. Oral creatine supplementation (up to 800 mg/kg/day) was begun. RESULTS: At age 9 years she demonstrated advanced academic performance. Partial recovery of cerebral creatine levels was demonstrated on MRS at 25 months of age. Brain MRS at 40 months of age revealed a creatine/NAA ratio of about 80% of that in age-matched controls. CONCLUSIONS: 8 years post initiation of oral creatine supplementation, patient demonstrates superior nonverbal and academic abilities, with average verbal skills. We emphasize that early diagnosis combined with early treatment onset of AGAT deficiency may lead to improvement of developmental outcome.
Subject(s)
Amidinotransferases/genetics , Amidinotransferases/metabolism , Creatine/administration & dosage , Creatine/blood , Developmental Disabilities , Amidinotransferases/deficiency , Brain/metabolism , Brain/pathology , Child , Developmental Disabilities/blood , Developmental Disabilities/diet therapy , Dietary Supplements , Female , Glycine/analogs & derivatives , Glycine/blood , Glycine/urine , Humans , MutationABSTRACT
Urinary organic acids, plasma amino acids and acylcarnitine profile analyses are the main tools used to diagnose inborn errors of metabolisms (IEMs). However, without metabolic decompensation, these parameters are often not helpful. On the other hand, in cases of IEM, acylglycines are consistently raised even when patients appear to be in remission. This study aims to set-up a simple liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of urine acylglycines, complementary to organic acid and acylcarnitine profiles, for the diagnosis of IEM. In addition, local reference intervals for various acylglycines are established by using this method. Acylglycines were isolated by solid-phase extraction, derivatized with n-butanol, separated by HPLC, and detected by ESI-MS/MS. Acylglycines were quantified with deuterated internal standards. Mean recoveries of acylglycines ranged from 90.2 to 109.3%. Within- and between-run imprecisions for all acylglycines have CVs less than 10%. Linear regression coefficients were greater than 0.99. Reference intervals were established according to CLSI guidelines by analyzing 204 samples from apparently healthy individuals less than 18 years of age. The distributions of AG in the "normal" urine were skewed towards the right. After log transformation, all the results were normally distributed. Partitioning into age group reference intervals was not indicated, according to the Harris and Boyd approach. In this context, a single reference interval for each acylglycine could be used. This method of urine acylglycines analysis is a powerful diagnostic tool, complementary to urine organic acids and plasma acylcarnitine profiling, for detecting certain inborn errors of metabolism.
Subject(s)
Glycine/analogs & derivatives , Glycine/urine , Metabolism, Inborn Errors/urine , 1-Butanol/chemistry , Acylation , Adolescent , Asian People , Calibration , Carnitine/analogs & derivatives , Carnitine/urine , Child , Child, Preschool , Chromatography, High Pressure Liquid , Deuterium , Female , Humans , Infant , Infant, Newborn , Male , Metabolism, Inborn Errors/diagnosis , Reference Values , Sensitivity and Specificity , Solid Phase Extraction , Spectrometry, Mass, Electrospray Ionization , Tandem Mass SpectrometryABSTRACT
A 4-year-old female with history of developmental regression and autistic features was diagnosed with guanidinoacetate methyltransferase deficiency at age 21 months. Upon treatment, she showed improvements in her developmental milestones, sensorial-neural hearing loss and brain atrophy on cranial-MRI. The creatine/choline ratio increased 82% in basal ganglia and 88% in white matter on cranial MR-spectroscopy. The CSF guanidinoacetate decreased 80% after six months of ornithine and creatine supplementation and an additional 8% after 18 months of additional arginine restricted diet. We report the most favorable clinical and biochemical outcome on treatment in our patient.
Subject(s)
Arginine/deficiency , Diet , Guanidinoacetate N-Methyltransferase/deficiency , Metabolism, Inborn Errors/diet therapy , Metabolism, Inborn Errors/enzymology , Child, Preschool , Female , Glycine/analogs & derivatives , Glycine/blood , Glycine/cerebrospinal fluid , Glycine/urine , Guanidinoacetate N-Methyltransferase/metabolism , Humans , Infant , Infant, Newborn , Treatment OutcomeABSTRACT
Proline is an amino acid with an essential role for primary metabolism and physiologic functions. Hyperprolinemia results from the deficiency of specific enzymes for proline catabolism, leading to tissue accumulation of this amino acid. Hyperprolinemic patients can present neurological symptoms and brain abnormalities, whose aetiopathogenesis is poorly understood. This review addresses some of the findings obtained, mainly from animal studies, indicating that high proline levels may be associated to neuropathophysiology of some disorders. In this context, it has been suggested that energy metabolism deficit, Na(+),K(+)-ATPase, kinase creatine, oxidative stress, excitotoxicity, lipid content, as well as purinergic and cholinergic systems are involved in the effect of proline on brain damage and spatial memory deficit. The discussion focuses on the relatively low antioxidant defenses of the brain and the vulnerability of neural tissue to reactive species. This offers new perspectives for potential therapeutic strategies for this condition, which may include the early use of appropriate antioxidants as a novel adjuvant therapy, besides the usual treatment based on special diets poor in proline.