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1.
Bioengineered ; 13(1): 1049-1061, 2022 01.
Article in English | MEDLINE | ID: mdl-35112970

ABSTRACT

As a leading complication of sepsis, sepsis-induced cardiac dysfunction (SICD) contributed to the high mortality of patients with sepsis. Long non-coding RNA (LncRNA) LINC00472 has been reported to be in sepsis-induced disease. Nonetheless, its biological function and underlying molecular in SICD remain largely unknown. In this study, in vivo and in vitro SICD models were established via LPS treatment. H&E staining was employed for the evaluation of myocardial injury. ELISA assay was performed to detect cardiac Troponin I (cTnI), creatine kinase-MB (CK-MB), interleukin (IL)-1ß, and tumor necrosis factor-α (TNF-α) levels. Cardiomyocyte viability and apoptosis were assessed via CCK-8 and flow cytometry assays. The transcriptional regulation of YY1 on LINC00472 was demonstrated via ChIP assay. Besides, the interaction between YY1 and LINC00472, as well as the association between miR-335-3p and LINC00472 or MAOA were verified via luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Herein, highly expressed LINC00472 was observed in both in vivo and in vitro SICD models. LINC00472 knockdown substantially attenuated LPS-induced inhibition on cardiomyocyte viability and reversed cardiomyocyte apoptosis and inflammatory response mediated by LPS treatment. YY1 induced LINC00472 upregulation, thereby promoting cardiomyocyte dysfunction induced by LPS. In addition, MAOA upregulation or miR-335-3p inhibition could partly reverse the suppressive effect on LPS-induced cardiomyocyte dysfunction mediated by LINC00472 knockdown. Based on our results, it seemed that YY1-activated LINC00472 might contribute to SICD progression via the miR-335-3p/MAOA pathway.


Subject(s)
Heart Diseases , MicroRNAs , RNA, Long Noncoding , Sepsis , YY1 Transcription Factor , Animals , Disease Models, Animal , Heart Diseases/etiology , Heart Diseases/genetics , Heart Diseases/metabolism , Humans , Male , Mice , Mice, Inbred C57BL , MicroRNAs/genetics , MicroRNAs/metabolism , Monoamine Oxidase/genetics , Monoamine Oxidase/metabolism , RNA, Long Noncoding/genetics , RNA, Long Noncoding/metabolism , Sepsis/complications , Sepsis/genetics , Sepsis/metabolism , YY1 Transcription Factor/genetics , YY1 Transcription Factor/metabolism
2.
Nutrients ; 13(7)2021 Jun 23.
Article in English | MEDLINE | ID: mdl-34201625

ABSTRACT

Omega-3 (n-3) polyunsaturated fatty acids (PUFA) and their metabolites have long been recognized to protect against inflammation-related diseases including heart disease. Recent reports present conflicting evidence on the effects of n-3 PUFAs on major cardiovascular events including death. While some studies document that n-3 PUFA supplementation reduces the risk for heart disease, others report no beneficial effects on heart disease composite primary outcomes. Much of this heterogeneity may be related to the genetic variation in different individuals/populations that alters their capacity to synthesize biologically active n-3 and omega 6 (n-6) PUFAs and metabolites from their 18 carbon dietary precursors, linoleic acid (LA, 18:2 n-6) and alpha-linolenic (ALA, 18:3, n-3). Here, we discuss the role of a FADS gene-by-dietary PUFA interaction model that takes into consideration dietary exposure, including the intake of LA and ALA, n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in determining the efficacy of n-3 PUFA supplementation. We also review recent clinical trials with n-3 PUFA supplementation and coronary heart disease in the context of what is known about fatty acid desaturase (FADS) gene-by-dietary PUFA interactions. Given the dramatic differences in the frequencies of FADS variants that impact the efficiency of n-3 and n-6 PUFA biosynthesis, and their downstream signaling products among global and admixture populations, we conclude that large clinical trials utilizing "one size fits all" n-3 PUFA supplementation approaches are unlikely to show effectiveness. However, evidence discussed in this review suggests that n-3 PUFA supplementation may represent an important opportunity where precision interventions can be focused on those populations that will benefit the most from n-3 PUFA supplementation.


Subject(s)
Clinical Trials as Topic , Diet , Dietary Supplements , Fatty Acids, Omega-3/pharmacology , Heart Diseases/genetics , Heart Diseases/therapy , Genetic Variation , Humans
3.
Mol Biol Rep ; 48(1): 743-761, 2021 Jan.
Article in English | MEDLINE | ID: mdl-33275195

ABSTRACT

Obesity is growing at an alarming rate, which is characterized by increased adipose tissue. It increases the probability of many health complications, such as diabetes, arthritis, cardiac disease, and cancer. In modern society, with a growing population of obese patients, several individuals have increased insulin resistance. Herbal medicines are known as the oldest method of health care treatment for obesity-related secondary health issues. Several traditional medicinal plants and their effective phytoconstituents have shown anti-diabetic and anti-adipogenic activity. Adipose tissue is a major site for lipid accumulation as well as the whole-body insulin sensitivity region. 3T3-L1 cell line model can achieve adipogenesis. Adipocyte characteristics features such as expression of adipocyte markers and aggregation of lipids are chemically induced in the 3T3-L1 fibroblast cell line. Differentiation of 3T3-L1 is an efficient and convenient way to obtain adipocyte like cells in experimental studies. Peroxisome proliferation activated receptor γ (PPARγ) and Cytosine-Cytosine-Adenosine-Adenosine-Thymidine/Enhancer-binding protein α (CCAAT/Enhancer-binding protein α or C/EBPα) are considered to be regulating adipogenesis at the early stage, while adiponectin and fatty acid synthase (FAS) is responsible for the mature adipocyte formation. Excess accumulation of these adipose tissues and lipids leads to obesity. Thus, investigating adipose tissue development and the underlying molecular mechanism is important in the therapeutical approach. This review describes the cellular mechanism of 3T3-L1 fibroblast cells on potential anti-adipogenic herbal bioactive compounds.


Subject(s)
Anti-Obesity Agents/therapeutic use , Arthritis/prevention & control , Diabetes Mellitus/prevention & control , Heart Diseases/prevention & control , Neoplasms/prevention & control , Obesity/drug therapy , Phytochemicals/therapeutic use , 3T3-L1 Cells , Adipogenesis/drug effects , Adipogenesis/genetics , Adiponectin/genetics , Adiponectin/metabolism , Adipose Tissue/drug effects , Adipose Tissue/metabolism , Adipose Tissue/pathology , Animals , Anti-Obesity Agents/chemistry , Arthritis/etiology , Arthritis/genetics , Arthritis/pathology , CCAAT-Enhancer-Binding Proteins/genetics , CCAAT-Enhancer-Binding Proteins/metabolism , Diabetes Mellitus/etiology , Diabetes Mellitus/genetics , Diabetes Mellitus/pathology , Fatty Acid Synthases/genetics , Fatty Acid Synthases/metabolism , Gene Expression Regulation , Heart Diseases/etiology , Heart Diseases/genetics , Heart Diseases/pathology , Humans , Insulin Resistance , Mice , Neoplasms/etiology , Neoplasms/genetics , Neoplasms/pathology , Obesity/complications , Obesity/genetics , Obesity/pathology , PPAR gamma/genetics , PPAR gamma/metabolism , Phytochemicals/chemistry
4.
Toxicol Lett ; 336: 39-49, 2021 Jan 01.
Article in English | MEDLINE | ID: mdl-33166663

ABSTRACT

Regorafenib (RGF) has a great success in the treatment of colorectal cancer, gastrointestinal stromal tumours and hepatocellular carcinoma by inhibiting angiogenic, stromal and oncogenic kinases. However, RGF can induce life-threatening cardiotoxicity including hypertension and cardiac ischemia/infarction. The molecular mechanism of the adverse effects has not been elucidated. Mitochondrial dysfunction is one of the major causes of cardiac diseases since cardiac cells highly need ATP for their contractility. Therefore, we aimed to investigate molecular mechanisms of RGF-induced cardiac adverse effects using H9c2 cell model by focusing on mitochondria. Cells were treated with 0-20 µM RGF for 48 and 72 h. According to our results, RGF inhibited cell proliferation and decreased the ATP content of the cells depending on the exposure time and concentration. Loss of mitochondrial membrane potential was also observed at high dose. Mitochondrial fusion/fission genes and antioxidant SOD2 (superoxide dismutase) gene expression levels increased at high doses in both treatments. Mitochondrial DNA content decreased as exposure time and concentration increased. Also, protein expression levels of mitochondrial complex I and V have reduced and stress protein HSP70 level has increased following RGF treatment. Structural abnormalities in mitochondria was seen with transmission electron microscopy at the applied higher doses. Our findings suggest that RGF-induced cardiotoxicity may be associated with mitochondrial damage in cardiac cells.


Subject(s)
Antineoplastic Agents/toxicity , Heart Diseases/chemically induced , Mitochondria, Heart/drug effects , Mitochondrial Dynamics/drug effects , Myocytes, Cardiac/drug effects , Phenylurea Compounds/toxicity , Pyridines/toxicity , Adenosine Triphosphate/metabolism , Animals , Cardiotoxicity , Cell Line , Cell Proliferation/drug effects , DNA, Mitochondrial/genetics , DNA, Mitochondrial/metabolism , Dose-Response Relationship, Drug , HSP70 Heat-Shock Proteins/metabolism , Heart Diseases/genetics , Heart Diseases/metabolism , Heart Diseases/pathology , Membrane Potential, Mitochondrial/drug effects , Mitochondria, Heart/genetics , Mitochondria, Heart/metabolism , Mitochondria, Heart/pathology , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Rats , Time Factors
5.
J Inherit Metab Dis ; 44(3): 740-750, 2021 05.
Article in English | MEDLINE | ID: mdl-33145772

ABSTRACT

Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disorder caused by mutations in the IDUA gene, that codifies the alpha-L-iduronidase enzyme, which deficiency leads to storage of glycosaminoglycans, with multiple clinical manifestations. One of the leading causes of death in MPS I patients are cardiac complications such as cardiac valve thickening, conduction abnormalities, myocardial dysfunction, and cardiac hypertrophy. The mechanism leading to cardiac dysfunction in MPS I is not entirely understood. In a previous study, we have demonstrated that losartan and propranolol improved the cardiac function in MPS I mice. Thus, we aimed to investigate whether the pathways influenced by these drugs may modulate the cardiac remodeling process in MPS I mice. According to our previous observation, losartan and propranolol restore the heart function, without altering valve thickness. MPS I mice presented reduced activation of AKT and ERK1/2, increased activity of cathepsins, but no alteration in metalloproteinase activity was observed. Animals treated with losartan showed a reduction in cathepsin activity and restored ERK1/2 activation. While both losartan and propranolol improved heart function, no mechanistic evidence was found for propranolol so far. Our results suggest that losartan or propranolol could be used to ameliorate the cardiac disease in MPS I and could be considered as adjuvant treatment candidates for therapy optimization.


Subject(s)
Heart Diseases/pathology , Losartan/pharmacology , MAP Kinase Signaling System/drug effects , Mucopolysaccharidosis I/drug therapy , Ventricular Remodeling/drug effects , Animals , Disease Models, Animal , Echocardiography , Female , Heart Diseases/drug therapy , Heart Diseases/genetics , Iduronidase/genetics , MAP Kinase Signaling System/genetics , Male , Mice , Mice, Inbred C57BL , Mucopolysaccharidosis I/genetics , Mucopolysaccharidosis I/pathology , Mutation
6.
Toxicol Lett ; 318: 1-11, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31618665

ABSTRACT

Triptolide (TP), a principal bioactive component extracted from traditional Chinese medicine Tripterygium wilfordii Hook. F. (TWHF), has attracted wide attention of its therapeutic effects on inflammation and autoimmune diseases. However, the therapeutic application of TP is hindered by severe cardiomyocyte toxicity and narrow therapeutic window. We previously identified that the p53 was an indispensable contributor in TP-induced myocardial injury. p53 has an inhibitory effect on IKKß-NF-κB pathway that regulates glucose transporters (GLUT) expression. Based on these evidences, we speculate that p53 mediates TP-disturbed glucose uptake by blocking IKKß-NF-κB signaling. This study focused on the effect of TP on cardiac glucose uptake and the role of p53 in glucose metabolism in cardiomyocytes, and p53 -/- mice. TP treatment depressed glucose consumption and ATP production resulting in myocardial damage. Incubation with ATP (5 mM) remarkably decreased the cellular damage. Immunoblotting and immunofluorescence identified that TP suppressed glucose uptake by restricting IKKß-NF-κB signaling activation, GLUT1 and GLUT4 expression. p53 inhibition alleviated the cell damage and the compromise of glucose uptake. Mechanistically, p53 antagonist PFTα abolished TP-induced the inhibition of IKKß, IκBα phosphorylation, p65 nuclear translocation, and GLUT1, GLUT4 expression. Consistently, in acute heart injury models, p53 deficiency upregulated IKKß-NF-κB activation and GLUT1, GLUT4 protein levels which was also indicated as amelioration of heart histological injury after 1.2 mg kg-1 TP administration. The present findings indicate that TP-induced p53 overactivation suppresses glucose uptake by inhibiting IKKß-NF-κB pathway and downregulating NF-κB-dependent GLUT1 and GLUT4 expression.


Subject(s)
Diterpenes/toxicity , Glucose/metabolism , Heart Diseases/chemically induced , I-kappa B Kinase/metabolism , Myocytes, Cardiac/drug effects , NF-kappa B/metabolism , Phenanthrenes/toxicity , Tumor Suppressor Protein p53/metabolism , Animals , Apoptosis/drug effects , Cardiotoxicity , Cell Line , Energy Metabolism/drug effects , Epoxy Compounds/toxicity , Glucose Transporter Type 1/genetics , Glucose Transporter Type 1/metabolism , Glucose Transporter Type 4/genetics , Glucose Transporter Type 4/metabolism , Heart Diseases/genetics , Heart Diseases/metabolism , Heart Diseases/pathology , Mice, Knockout , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Rats, Sprague-Dawley , Signal Transduction/drug effects , Tumor Suppressor Protein p53/deficiency , Tumor Suppressor Protein p53/genetics
7.
J Ethnopharmacol ; 246: 112210, 2020 Jan 10.
Article in English | MEDLINE | ID: mdl-31479707

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: Compound Danshen tablet, an herbal preparation consisting of salviae miltiorrhizae, notoginseng and borneolum, is extensively employed clinically to treat angina pectoris, coronary arteriosclerosis and significantly improve microcirculation. AIM OF THE STUDY: To reveal the potential underlying cardioprotective mechanism(s) in isoproterenol-induced myocardial injury in high-fat-diet fed mice. MATERIALS AND METHODS: Cardiac transcriptomics was analyzed by Illumina mRNA-Seq sequencing. The restored cardiovascular diseases (CVD)-related genes by Compound Danshen tablet were validated by quantitative real time polymerase chain reaction (qRT-PCR). Furthermore, Cardiac metabolomics were also performed using gas chromatography-mass spectrometry. RESULTS: From the transcriptomics study, we found the levels of 24 up-regulated and 44 down-regulated genes in the control compared to model groups. Among them, seven gene levels were restored by treatment of Compound Danshen tablet. Four CVD-related genes at the mRNA level (Sprr1a, Ppp1r3c, Bmp10 and Hspa1b) were validated successfully by qRT-PCR. From the metabolomics study, 37 differentially expressed metabolites were identified between the control and model groups. Among them, 21 metabolites were restored by treatment of Compound Danshen tablet. These altered metabolites are involved in glucose metabolism, fatty acid metabolism and amino acid metabolism. CONCLUSION: These genes and metabolites might provide clues for further molecular mechanistic study of Compound Danshen tablet.


Subject(s)
Cardiotonic Agents/pharmacology , Cardiotonic Agents/therapeutic use , Drugs, Chinese Herbal/pharmacology , Drugs, Chinese Herbal/therapeutic use , Heart Diseases/genetics , Heart Diseases/metabolism , Animals , Camphanes , Diet, High-Fat , Gene Expression Profiling , Heart Diseases/chemically induced , Heart Diseases/drug therapy , Isoproterenol , Male , Metabolomics , Mice, Inbred C57BL , Myocardium/metabolism , Panax notoginseng , Salvia miltiorrhiza , Tablets , Transcriptome/drug effects
8.
Dis Markers ; 2020: 6645588, 2020.
Article in English | MEDLINE | ID: mdl-33425072

ABSTRACT

BACKGROUND: Breast cancer treatment is associated with the occurrence of various cardiac adverse events. One of the mechanisms associated with cardiotoxicity is oxidative stress, against which cells are protected by antioxidative enzymes. Genetic variability of antioxidative enzymes can affect enzyme activity or expression, which modifies the ability of cells to defend themselves against oxidative stress and could consequently contribute to the occurrence of treatment-related cardiotoxicity. Our aim was to evaluate the association of common polymorphisms in antioxidative genes with cardiotoxicity after adjuvant radiotherapy (RT) in HER2-positive breast cancer patients. METHODS: Our retrospective study included 101 HER2-positive early breast cancer patients who received trastuzumab and adjuvant RT. We isolated DNA from buccal swabs and used competitive allele-specific PCR for genotyping of PON1 rs854560 and rs662, GSTP1 rs1138272 and rs1695, SOD2 rs4880, CAT rs1001179, and HIF1 rs1154965 polymorphisms. N-terminal pro B-type natriuretic peptide (NT-proBNP), left ventricular ejection fraction, and NYHA class were used as markers of cardiotoxicity. We used logistic regression to evaluate the association of genetic factors with markers of cardiotoxicity. RESULTS: Carriers of at least one polymorphic PON1 rs854560 allele were less likely to have increased NT-proBNP (OR = 0.34; 95% CI = 0.15-0.79; P = 0.012), even after adjustment for age (OR = 0.35; 95% CI = 0.15-0.83; P = 0.017). Carriers of at least one polymorphic PON1 rs662 allele were more likely to have increased NT-proBNP (OR = 4.44; 95% CI = 1.85-10.66; P = 0.001), even after adjustment for age (OR = 5.41; 95% CI = 2.12-13.78; P < 0.001). GSTP1 rs1695 was also associated with decreased NT-proBNP in the multivariable analysis (P = 0.026), while CAT rs1001179 was associated with NYHA class in the univariable (P = 0.012) and multivariable analysis (P = 0.023). CONCLUSION: In our study, polymorphisms PON1 rs662 and rs854560, CAT rs1001179, and GSTP1 rs1695 were significantly associated with the occurrence of cardiac adverse events after adjuvant RT and could serve as biomarkers contributing to treatment personalization.


Subject(s)
Aryldialkylphosphatase/genetics , Breast Neoplasms/radiotherapy , Catalase/genetics , Glutathione S-Transferase pi/genetics , Heart Diseases/genetics , Polymorphism, Single Nucleotide , Adult , Antineoplastic Agents/adverse effects , Antineoplastic Agents/therapeutic use , Antineoplastic Agents/toxicity , Breast Neoplasms/drug therapy , Cardiotoxicity/blood , Cardiotoxicity/etiology , Cardiotoxicity/genetics , Female , Heart Diseases/blood , Heart Diseases/etiology , Humans , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , Middle Aged , Natriuretic Peptide, Brain/blood , Radiotherapy, Adjuvant/adverse effects , Receptor, ErbB-2/genetics , Superoxide Dismutase/genetics , Trastuzumab/adverse effects , Trastuzumab/therapeutic use , Trastuzumab/toxicity
9.
J Food Biochem ; 43(8): e12952, 2019 08.
Article in English | MEDLINE | ID: mdl-31368573

ABSTRACT

This study compared the effect of low-fat diet (LFD) and high-fat diet rich in corn oil (HFD-CO) on left ventricular (LV) fibrosis in rats and examined their effect of angiotensin II (ANG II), JAK/STAT, and TGF-1ß/smad3 pathways. As compared to LFD which didn't affect any of the measured parameters, HFD-CO-induced type 2 diabetes phenotype and increased LV collagen synthesis. Mechanistically, it increased LV levels of ROS, ANG II, ACE, IL-6, s-IL-6Rα, TGF-ß1, Smad-3, and activities of JAK1/2 and STAT1/3. AG490, a JAK2 inhibitor, partially ameliorated these effect while Losartan, an AT1 inhibitor completely abolished collagen synthesis. However, with both treatments, levels of ANG II, IL-6, and s-IL-6Rα, and activity of JAK1/STAT3 remained high, all of which were normalized by co-administration of NAC or IL-6 neutralizing antibody. In conclusion: HFD-CO enhances LV collage synthesis by activation of JAK1/STAT3/ANG II/TGF-1ß/smad3 pathway. PRACTICAL APPLICATIONS: We report that chronic consumption of a high-fat diet rich in corn oil (HFD-CO) induces diabetes mellitus phenotype 2 associated with left ventricular (LV) cardiac fibrosis in rats. The findings of this study show that HFD-CO, and through the increasing generation of ROS and IL-6 levels and shedding, could activate LV JAK1/2-STAT1/3  and  renin-angiotensin system (RAS) signaling pathways, thus creating a positive feedback between the two which ultimately leads to activation of TGF-1ß/Smad3 fibrotic pathway. Herein, we also report a beneficial effect of the antioxidant, NAC, or IL-6 neutralizing antibody in preventing such adverse effects of such HFD-CO. However, this presents a warning message to the current sudden increase in idiopathic cardiac disorders, especially with the big shift in our diets toward n-6 PUFA.


Subject(s)
Corn Oil/adverse effects , Diet, High-Fat/adverse effects , Fibrosis/metabolism , Heart Diseases/metabolism , Reactive Oxygen Species/metabolism , Angiotensin II/genetics , Angiotensin II/metabolism , Animals , Corn Oil/metabolism , Fibrosis/etiology , Fibrosis/genetics , Heart Diseases/etiology , Heart Diseases/genetics , Heart Ventricles/metabolism , Humans , Interleukin-6/genetics , Interleukin-6/metabolism , Janus Kinase 2/genetics , Janus Kinase 2/metabolism , Male , Rats , Rats, Wistar , STAT3 Transcription Factor/genetics , STAT3 Transcription Factor/metabolism , Signal Transduction , Smad3 Protein/genetics , Smad3 Protein/metabolism , Transforming Growth Factor beta1/genetics , Transforming Growth Factor beta1/metabolism
10.
Circulation ; 140(7): 595-610, 2019 08 13.
Article in English | MEDLINE | ID: mdl-31403841

ABSTRACT

Inheritable cardiac disorders, which may be associated with cardiomyopathic changes, are often associated with increased risk of sudden death in the young. Early linkage analysis studies in Mendelian forms of these diseases, such as hypertrophic cardiomyopathy and long-QT syndrome, uncovered large-effect genetic variants that contribute to the phenotype. In more recent years, through genotype-phenotype studies and methodological advances in genetics, it has become evident that most inheritable cardiac disorders are not monogenic but, rather, have a complex genetic basis wherein multiple genetic variants contribute (oligogenic or polygenic inheritance). Conversely, studies on genes underlying these disorders uncovered pleiotropic effects, with a single gene affecting multiple and apparently unrelated phenotypes. In this review, we explore these 2 phenomena: on the one hand, the evidence that variants in multiple genes converge to generate one clinical phenotype, and, on the other, the evidence that variants in one gene can lead to apparently unrelated phenotypes. Although multiple conditions are addressed to illustrate these concepts, the experience obtained in the study of long-QT syndrome, Brugada syndrome, and arrhythmogenic cardiomyopathy, and in the study of functions related to SCN5A (the gene coding for the α-subunit of the most abundant sodium channel in the heart) and PKP2 (the gene coding for the desmosomal protein plakophilin-2), as well, is discussed in more detail.


Subject(s)
Genetic Pleiotropy/genetics , Genetic Variation/genetics , Heart Diseases/diagnosis , Heart Diseases/genetics , Cardiomyopathy, Hypertrophic/diagnosis , Cardiomyopathy, Hypertrophic/genetics , Humans , Long QT Syndrome/diagnosis , Long QT Syndrome/genetics , Multifactorial Inheritance/genetics , NAV1.5 Voltage-Gated Sodium Channel/genetics , Plakophilins/genetics
11.
Clin Ther ; 41(6): 1097-1109, 2019 06.
Article in English | MEDLINE | ID: mdl-31053296

ABSTRACT

PURPOSE: The combination of warfarin and compound Danshen dripping pill (CDDP) is helpful for patients with both coronary heart disease (CHD) and atrial fibrillation (AF). The main adverse drug reaction of warfarin is bleeding because of its narrow therapeutic index. The safety of a combination therapy with warfarin and CDDP is always a concern. Our previous research showed that the combination of warfarin and CDDP improved the quality of life for patients with both CHD and AF. This study describes the changes in dose and concentration of warfarin necessary and evaluates bleeding risk when warfarin is given concomitantly with CDDP. METHODS: An ultra-performance liquid chromatography-MS/MS method with a chiral column was developed to assay the concentration of S-warfarin and R-warfarin in human plasma simultaneously. The method was applied to compare the concentration of warfarin in patients taking warfarin combined with CDDP and without CDDP. International normalized ratio (INR) values were monitored to evaluate bleeding risk. Paired t tests were then used to compare the dose and the concentration in 2 periods. Moreover, patients with VKORC1, CYP2C9*3, CYP4F2, EPHX1, and PROC gene polymorphisms were evaluated to determine interactions. FINDINGS: The results indicate that the dose of warfarin had no significant change with or without CDDP. Also, the peak concentrations of S-warfarin and total warfarin were significantly different in CYP4F2 C/C patients, but there was no significant difference identified in other genetic groups. No bleeding occurred in the study. IMPLICATIONS: The dose of warfarin would be sustainable when combined with CDDP, because CDDP did not affect concentration of warfarin significantly in most patients and the change of INR was not significant. CHINA CLINICAL TRIAL REGISTRY IDENTIFIER: ChiCTR-ONRC-13003523.


Subject(s)
Drugs, Chinese Herbal/pharmacokinetics , Heart Diseases/genetics , Polymorphism, Genetic/genetics , Warfarin , Camphanes , Cytochrome P450 Family 4/genetics , Heart Diseases/drug therapy , Hemorrhage/chemically induced , Hemorrhage/genetics , Herb-Drug Interactions , Humans , Panax notoginseng , Salvia miltiorrhiza , Warfarin/administration & dosage , Warfarin/adverse effects , Warfarin/pharmacokinetics , Warfarin/therapeutic use
12.
Pharmacol Res ; 141: 104-113, 2019 03.
Article in English | MEDLINE | ID: mdl-30579974

ABSTRACT

Dysregulated immune system has been implicated in the pathogenesis of various cardiovascular diseases. Therefore, development of pharmacological interventions targeting the immune system is promising. However, therapy with most common anti-inflammatory and immunomodulatory agents has proved challenging in the clinical translation. It has been proved that many herbal ingredients display definite therapeutic effects on preventing excessive inflammatory and immune responses. Here, we aim to systemically explore the immunomodulatory ability of herbal ingredients on the human heart tissue-specific immune dysfunction through a network pharmacology based approach. The approach matches gene expression data between herbal ingredients and human heart phenotype based on their immunological similarities. Firstly, 608 immunological signatures were produced from 304 transcriptional profiles of immunological cell state changes. Then, the immunological features of 28 human heart phenotypes and 102 herbal ingredients were constructed by calculating the enrichments of each immune signature in the transcriptional profiles of heart phenotypes and herbal ingredients, respectively. Finally, the likelihood that an herbal drug affects the immune system in a heart phenotype was qualified by calculating the immunological similarity between the herbal drug and the heart phenotype. This strategy integrating different types of OMICs data is expected to help create new opportunities for development of drugs targeting the immune dysfunction in heart disease.


Subject(s)
Heart Diseases/genetics , Heart Diseases/immunology , Immunologic Factors/pharmacology , Myocardium/immunology , Plant Preparations/pharmacology , Transcriptome/drug effects , Humans , Phenotype
13.
J Stroke Cerebrovasc Dis ; 28(2): 441-449, 2019 Feb.
Article in English | MEDLINE | ID: mdl-30409744

ABSTRACT

BACKGROUND: Cerebrovascular stroke is a common critical complication of sickle cell disease (SCD). Angiotensinogen (AGT) M235T gene polymorphism is associated with risk of ischemic stroke and cardiovascular disease. AIM: We investigated the potential association between angiotensinogen M235T gene polymorphism and susceptibility to cerebrovascular and cardiopulmonary complications in adolescents with SCD. METHODS: Forty-six patients with SCD in steady state were studied stressing on history of stroke, hydroxyurea/chelation therapy, hematological profile, and echocardiographic findings. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to detect AGT M235T gene polymorphism. Fifty sex- and age-matched healthy controls were enrolled for assessment of M235T gene polymorphism pattern. RESULTS: The distribution of AGT M235T gene polymorphism was similar between SCD patients and healthy controls. The frequency of T allele of AGT M235T gene polymorphism (TT and MT genotypes) was significantly higher among patients with history of manifest stroke (P < .001). Patients with TT and MT genotypes had higher incidence of cardiopulmonary complications (P = .041) as well as higher percentage of HbS (P < .001) and lower hemoglobin level (P = .008) compared with those with MM genotype. Serum ferritin, liver iron concentration, and cardiac T2* were not related to T alleles or genotypes. Logistic regression analysis revealed that M235T genotype was a significant independent factor related to the occurrence of stroke among patients with SCD (Odds Ratio 14.05, 95% confidence interval 3.82-28.91; P = .001). CONCLUSION: AGT M235T gene polymorphism may represent a genetic modifier to vascular morbidities in Egyptian patients with SCD.


Subject(s)
Anemia, Sickle Cell/genetics , Angiotensinogen/genetics , Cerebrovascular Disorders/genetics , Genes, Modifier , Heart Diseases/genetics , Lung Diseases/genetics , Polymorphism, Genetic , Adolescent , Age Factors , Anemia, Sickle Cell/diagnosis , Anemia, Sickle Cell/epidemiology , Case-Control Studies , Cerebrovascular Disorders/diagnosis , Cerebrovascular Disorders/epidemiology , Egypt/epidemiology , Female , Gene Frequency , Genetic Association Studies , Genetic Predisposition to Disease , Heart Diseases/diagnosis , Heart Diseases/epidemiology , Humans , Lung Diseases/diagnosis , Lung Diseases/epidemiology , Male , Phenotype , Risk Factors , Young Adult
14.
Toxicol Appl Pharmacol ; 348: 1-13, 2018 06 01.
Article in English | MEDLINE | ID: mdl-29653124

ABSTRACT

Doxorubicin (DOX) is a widely used anticancer drug that could be even more effective if its clinical dosage was not limited because of delayed cardiotoxicity. Beating stem cell-derived cardiomyocytes are a preferred in vitro model to further uncover the mechanisms of DOX-induced cardiotoxicity. Our objective was to use cultured induced-pluripotent stem cell(iPSC)-derived mouse cardiomyocytes (Cor.At) to investigate the effects of DOX on cell and mitochondrial metabolism, as well as on stress responses. Non-proliferating and beating Cor.At cells were treated with 0.5 or 1 µM DOX for 24 h, and morphological, functional and biochemical changes associated with mitochondrial bioenergetics, DNA-damage response and apoptosis were measured. Both DOX concentrations decreased ATP levels and SOD2 protein levels and induced p53-dependent caspase activation. However, differential effects were observed for the two DOX concentrations. The highest concentration induced a high degree of apoptosis, with increased nuclear apoptotic morphology, PARP-1 cleavage and decrease of some OXPHOS protein subunits. At the lowest concentration, DOX increased the expression of p53 target transcripts associated with mitochondria-dependent apoptosis and decreased transcripts related with DNA-damage response and glycolysis. Interestingly, cells treated with 0.5 µM DOX presented an increase in PDK4 transcript levels, accompanied by an increase in phospho-PDH and decreased PDH activity. This was accompanied by an apparent decrease in basal and maximal oxygen consumption rates (OCR) and in basal extracellular acidification rate (ECAR). Cells pre-treated with the PDK inhibitor dichloroacetate (DCA), with the aim of restoring PDH activity, partially recovered OCR and ECAR. The results suggest that the higher DOX concentration mainly induces p53-dependent apoptosis, whereas for the lower DOX concentration the cardiotoxic effects involve bioenergetic failure, unveiling PDH as a possible therapeutic target to decrease DOX cardiotoxicity.


Subject(s)
Antibiotics, Antineoplastic/toxicity , Doxorubicin/toxicity , Energy Metabolism/drug effects , Heart Diseases/chemically induced , Induced Pluripotent Stem Cells/drug effects , Myocytes, Cardiac/drug effects , Tumor Suppressor Protein p53/metabolism , Adenosine Triphosphate/metabolism , Animals , Apoptosis/drug effects , Cardiotoxicity , Cell Differentiation , Cell Line , DNA Damage , Dose-Response Relationship, Drug , Heart Diseases/genetics , Heart Diseases/metabolism , Heart Diseases/pathology , Induced Pluripotent Stem Cells/metabolism , Induced Pluripotent Stem Cells/pathology , Mice , Mitochondria, Heart/drug effects , Mitochondria, Heart/metabolism , Mitochondria, Heart/pathology , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Poly (ADP-Ribose) Polymerase-1/metabolism , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Pyruvate Dehydrogenase Acetyl-Transferring Kinase , Pyruvate Dehydrogenase Complex/metabolism , Superoxide Dismutase/metabolism , Tumor Suppressor Protein p53/genetics
15.
Circ Res ; 116(12): 1989-2004, 2015 Jun 05.
Article in English | MEDLINE | ID: mdl-26044252

ABSTRACT

Sudden cardiac death is a common cause of death in patients with structural heart disease, genetic mutations, or acquired disorders affecting cardiac ion channels. A wide range of platforms exist to model and study disorders associated with sudden cardiac death. Human clinical studies are cumbersome and are thwarted by the extent of investigation that can be performed on human subjects. Animal models are limited by their degree of homology to human cardiac electrophysiology, including ion channel expression. Most commonly used cellular models are cellular transfection models, which are able to mimic the expression of a single-ion channel offering incomplete insight into changes of the action potential profile. Induced pluripotent stem cell-derived cardiomyocytes resemble, but are not identical, adult human cardiomyocytes and provide a new platform for studying arrhythmic disorders leading to sudden cardiac death. A variety of platforms exist to phenotype cellular models, including conventional and automated patch clamp, multielectrode array, and computational modeling. Induced pluripotent stem cell-derived cardiomyocytes have been used to study long QT syndrome, catecholaminergic polymorphic ventricular tachycardia, hypertrophic cardiomyopathy, and other hereditary cardiac disorders. Although induced pluripotent stem cell-derived cardiomyocytes are distinct from adult cardiomyocytes, they provide a robust platform to advance the science and clinical care of sudden cardiac death.


Subject(s)
Death, Sudden, Cardiac , Induced Pluripotent Stem Cells/cytology , Myocytes, Cardiac/physiology , Animals , Cardiovascular Agents/pharmacology , Cell Differentiation/drug effects , Cells, Cultured , Clinical Trials as Topic/methods , Computer Simulation , Death, Sudden, Cardiac/etiology , Disease Models, Animal , Drug Evaluation, Preclinical/methods , Electrophysiology/methods , Forecasting , Heart Diseases/complications , Heart Diseases/genetics , Heart Diseases/pathology , Humans , Ion Channels/physiology , Long QT Syndrome/genetics , Long QT Syndrome/pathology , Long QT Syndrome/physiopathology , Models, Cardiovascular , Myocytes, Cardiac/cytology , Myocytes, Cardiac/drug effects , Organ Culture Techniques , Patch-Clamp Techniques/methods , Tachycardia, Ventricular/genetics , Tachycardia, Ventricular/pathology , Tachycardia, Ventricular/physiopathology
16.
Congenit Heart Dis ; 10(3): 219-25, 2015.
Article in English | MEDLINE | ID: mdl-25059817

ABSTRACT

OBJECTIVE: Recurrence risk of congenital heart disease (CHD) in families with an affected first-degree relative is increased as compared with the general population. Advances in genetic testing and evidence that preconceptual folic acid supplementation may decrease risk of CHD warrant preventative counseling for at-risk families. Our goal was to document patterns of preconceptual folic acid supplementation and recurrence risk counseling in at-risk families in order to identify opportunities for improved preventative care. DESIGN: Mothers referred for a fetal echocardiogram were prospectively enrolled. Cases were defined as mothers deemed to be at higher risk of having an affected fetus with CHD given an affected parent or affected previous pregnancy with CHD. Controls were defined as mothers with no prenatal risk factors. Mothers completed a validated questionnaire assessing use of folic acid supplementation and receipt of recurrence risk counseling. Chi-square analyses were performed to analyze questionnaire responses and demographic data. RESULTS: A total of 314 subjects participated (controls = 216, cases = 98). Cases took preconceptual folic acid supplementation more often than controls (P < .001), but only 55% started preconceptually. Maternal advanced education and counseling (P < .001) were associated with preconceptual supplementation, whereas complexity of CHD in the relative was not. While 70% of cases received some recurrence risk counseling, those with advanced education and complex CHD in the affected relative were more likely to receive counseling. Few at-risk cases interacted with genetic services (19%). CONCLUSIONS: At-risk mothers with lower education are less likely to take preconceptual folic acid supplementation or receive recurrence risk counseling. Health care providers should proactively provide this information to all at-risk patients and develop collaborations with genetic services.


Subject(s)
Directive Counseling , Folic Acid/therapeutic use , Heart Defects, Congenital/prevention & control , Heart Diseases/congenital , Heart Diseases/prevention & control , Preconception Care , Vitamin B Complex/therapeutic use , Adult , Female , Heart Defects, Congenital/genetics , Heart Diseases/genetics , Humans , Prospective Studies , Risk Assessment
17.
Am J Chin Med ; 41(5): 1125-36, 2013.
Article in English | MEDLINE | ID: mdl-24117073

ABSTRACT

Heart disease (HD) is associated with estrogen and therefore gender and menopausal status. In addition, clinical evidence shows that increased serum norepinephrine is found in patients with HD. Therefore, this study aimed to investigate the cardio-protective effect of genistein, a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, image data and results from western blotting shown that ISO treatment was capable of inducing cellular apoptosis, especially the mitochondrial dependent pathway. Treatment of genistein could suppress the expression of mitochondrial pro-apoptotic proteins including Bad, caspase-8, caspase-9, and caspase-3 in H9c2 treated with ISO. By contrast, several survival proteins were expressed in H9c2 treated with genistein, such as phosphor (p)-Akt, p-Bad, and p-Erk1/2. Furthermore, we confirmed that the protective role of genistein was partially mediated through the expression of Erk1/2, Akt, and NF κ B proteins by adding several pathway inhibitors. These in vitro data suggest that genistein may be a safe and natural SERM alternative to hormone therapy in cardio-protection.


Subject(s)
Apoptosis/drug effects , Apoptosis/genetics , Cardiotonic Agents , Down-Regulation/drug effects , Gene Expression/drug effects , Genistein/pharmacology , Isoproterenol/adverse effects , JNK Mitogen-Activated Protein Kinases/genetics , MAP Kinase Signaling System/genetics , MAP Kinase Signaling System/physiology , Mitogen-Activated Protein Kinase 3/genetics , Myocytes, Cardiac/pathology , NF-kappa B/genetics , Selective Estrogen Receptor Modulators , Up-Regulation/drug effects , p38 Mitogen-Activated Protein Kinases/genetics , Animals , Caspases/genetics , Caspases/metabolism , Cells, Cultured , Estrogens/physiology , Genistein/therapeutic use , Heart Diseases/drug therapy , Heart Diseases/genetics , Heart Diseases/prevention & control , JNK Mitogen-Activated Protein Kinases/physiology , Mitochondria/genetics , Mitochondria/pathology , Mitogen-Activated Protein Kinase 3/physiology , Molecular Targeted Therapy , NF-kappa B/physiology , Phytotherapy , Rats , Glycine max , bcl-Associated Death Protein/genetics , bcl-Associated Death Protein/metabolism , p38 Mitogen-Activated Protein Kinases/physiology
18.
Int J Biol Markers ; 28(4): e336-47, 2013 Dec 17.
Article in English | MEDLINE | ID: mdl-23999848

ABSTRACT

BACKGROUND: Anthracyclines are among the most active drugs against breast cancer, but can exert cardiotoxic effects eventually resulting in congestive heart failure (CHF). Identifying breast cancer patients at high risk of developing cardiotoxicity after anthracycline therapy would be of value in guiding the use of these agents. AIMS: We determined whether polymorphisms in the renin-angiotensin-aldosterone system (RAAS) and in the glutathione S-transferase (GST) family of phase II detoxification enzymes might be useful predictors of left ventricular ejection fraction (LVEF) kinetics and risk of developing CHF. We sought correlations between the development of cardiotoxicity and gene polymorphisms in 48 patients with early breast cancer treated with adjuvant anthracycline chemotherapy. METHODS: We analyzed the following polymorphisms: p.Met235Thr and p.Thr174Met in angiotensinogen (AGT), Ins/Del in angiotensin-converting enzyme (ACE), A1166C in angiotensin II type-1 receptor (AGTR1A), c.-344T>C in aldosterone synthase (CYP11B2), p.Ile105Val in GSTP1. Additionally, we analyzed the presence or absence of the GSTT1 and GSTP1 genes. A LVEF <50% was detected at least once during the 3 years of follow-up period in 13 out of 48 patients (27.1%). CONCLUSION: RAAS gene polymorphisms were not significantly associated with the development of cardiotoxicity. GSTM1may be useful as a biomarker of higher risk of cardiotoxicity, as demonstrated in our cohort of patients (p=0.147).


Subject(s)
Anthracyclines/adverse effects , Breast Neoplasms/drug therapy , Breast Neoplasms/genetics , Glutathione Transferase/genetics , Heart Diseases/chemically induced , Heart Diseases/genetics , Renin-Angiotensin System/genetics , Adult , Aged , Angiotensinogen/genetics , Anthracyclines/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/adverse effects , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Breast Neoplasms/enzymology , Breast Neoplasms, Male/drug therapy , Breast Neoplasms, Male/genetics , Chemotherapy, Adjuvant , Cyclophosphamide/administration & dosage , Cyclophosphamide/adverse effects , Epirubicin/administration & dosage , Epirubicin/adverse effects , Female , Fluorouracil/administration & dosage , Fluorouracil/adverse effects , Heart Diseases/enzymology , Humans , Isoenzymes/genetics , Male , Middle Aged , Polymorphism, Single Nucleotide , Prospective Studies
19.
Zhen Ci Yan Jiu ; 38(2): 87-92, 2013 Apr.
Article in Chinese | MEDLINE | ID: mdl-23819208

ABSTRACT

OBJECTIVE: To observe the effect of electroacupuncture (EA) on the expression of myocardial 1-phosphatidylinositol 3-kinase (PI 3 K), hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in rats with cerebral-cardiac syndrome (CCS), so as to reveal its mechanism underlying reducing ischemic myocardial injury. METHODS: Forty SD rats were randomly and equally divided into sham-operation, model, EA and non-acupoint (the lateral-superior side of the hip) groups (10 rats/group). CCS model was established by injection of collagenase (1 U/microL) and heparin (7 U/microL) into the right caudate nucleus. Following modeling, EA (1.5 mA, 2 Hz, 20 min) was applied to "Shuigou" (GV 26), "Fengfu" (GV 16), "Neiguan" (PC 6) and "Xinshu" (BL 15) acupoints, once daily for three consecutive days. The expression levels of PI 3 K,HIF-1alpha and VEGF in the myocardium were detected by immunohistochemistry. RESULTS: Compared with the sham-operation group, the expression levels of myocardial PI 3 K, HIF-1a and VEGF proteins were significantly increased in the model group (P<0.01). While in comparison with the model group, there were little increase in the non-acupoint group (P>0.05) and considerable increase in the expression levels of the 3 myocardial proteins in the EA group (P<0.05). CONCLUSION: EA intervention has a function in upregulating the expression of myocardial VEGF, HIF-1alpha and PI 3 K proteins in CCS rats, which maybe contribute to its protective effect on ischaemic myocardial injury.


Subject(s)
Cerebellar Diseases/genetics , Cerebellar Diseases/therapy , Electroacupuncture , Heart Diseases/genetics , Heart Diseases/therapy , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , Phosphatidylinositol 3-Kinase/genetics , Vascular Endothelial Growth Factor A/genetics , Acupuncture Points , Animals , Cerebellar Diseases/metabolism , Female , Heart Diseases/metabolism , Humans , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Male , Phosphatidylinositol 3-Kinase/metabolism , Rats , Rats, Sprague-Dawley , Vascular Endothelial Growth Factor A/metabolism
20.
Gac Med Mex ; 149(2): 212-9, 2013.
Article in Spanish | MEDLINE | ID: mdl-23652188

ABSTRACT

Heart development consists in a group of complex and specific morfogenetic interactions, that requires the proper activity of each factor implicated in this process. Congenital heart defects (CHD) are a group of multifactorial complex diseases with environmental and genetic factors playing important roles. There is not an exact relation between molecular mechanisms and morphological defects in CHD, because in most of the cases the proper development of an anatomical structure implies the adequate function of several pathways that may depend of the action of different genes. This review summarizes the genetic factors implied in the normal heart development and the most common gene mutations associated with CHD.


Subject(s)
Heart Defects, Congenital/genetics , Heart Diseases/congenital , Heart Diseases/genetics , Heart/anatomy & histology , Humans
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