Unraveling the potential of bacterial phytases for sustainable management of phosphorous.

Phosphorous actively participates in numerous metabolic and regulatory activities of almost all living organisms including animals and humans. Therefore, it is considered as an essential macronutrient required supporting their proper growth. On contrary, phytic acid (PA), an antinutritional substance, is widely known for its strong affinity to chelate essential mineral ions including PO4 3- , Ca2+ , Fe2+ , Mg2+ , and Zn2+ . Being one the major reservoir of PO4 3- ions, PA has great potential to bind PO4 3- ions in diverse range of foods. Once combined with P, PA transforms into an undigested and insoluble complex namely phytate. Produced phytate leads to a notable reduction in the bioavailability of P due to negligible activity of phytases in monogastric animals and humans. This highlights the importance and consequent need of enhancement of phytase level in these life forms. Interestingly, phytases, catalyzing the breakdown of phytate complex and recycling the phosphate into ecosystem to its available form, have naturally been reported in a variety of plants and microorganisms over past few decades. In pursuit of a reliable solution, the focus of this review is to explore the keynote potential of bacterial phytases for sustainable management of phosphorous via efficient utilization of soil phytate. The core of the review covers detailed discussion on bacterial phytases along with their widely reported applications viz. biofertilizers, phosphorus acquisition, and plant growth promotion. Moreover, meticulous description on fermentation-based strategies and future trends on bacterial phytases have also been included.

Effect of particle size and weight fraction of SiC on the mechanical, tribological, morphological, and structural properties of Al-5.6Zn-2.2Mg-1.3Cu composites using RSM: fabrication, characterization, and modelling.

Stir-casting was employed to create Al-5.6Zn-2.2Mg-1.3Cu composites with particle sizes ranging from 30 to 90 µm and a weight fraction of 5-15 SiC articles. The mechanical and wear properties of the material have been assessed. The wear-behaviour of Al-5.6Zn-2.2Mg-1.3Cu composites was investigated using dry pin-on-disc wear testing. Various loads (20 N-60 N), speeds (2 m/s-6 m/s), and sliding-distances were used in the sliding wear experiments (2000 m-4000 m). In the experimental process, XRD, SEM, and EDX were used to characterize the microstructures and materials of diverse composites. Uniform dispersion of the SiC particles is clearly observed in the SEM image. The micro hardness of SiC particles increases by 13% when the weight percent of SiC particles is increased from 5% to 15%. SiC particles outperform tiny SiC particles in terms of wear-resistance. With increasing load, the particular wear-rate showed an increasing trend (20-60 N). The wear-rate of the composite lowers as the weight percentage reinforcement increases (wt. 5% to wt. 15%), and the wear-rate of the composite increases when the particle-size (30 µm-90 µm) increases. The results demonstrated that composites supplemented with coarse SiC particles outperform tiny SiC particles in terms of wear resistance.

Neurotoxic effects of environmental contaminants-measurements, mechanistic insight, and environmental relevance.

Pollution is a significant and growing concern for any population regardless of age because these environmental contaminants exhibit different neurodegenerative effects on persons of different ages. These environmental contaminants are the products of human welfare projects like industry, automobile exhaust, clinical and research laboratory extrudes, and agricultural chemicals. These contaminants are found in various forms in environmental matrices like nanoparticles, particulate matter, lipophilic vaporized toxicants, and ultrafine particulate matter. Because of their small size, they can easily cross blood-brain barriers or use different cellular mechanisms for assistance. Other than this, these contaminants cause an innate immune response in different cells of the central nervous system and cause neurotoxicity. Considering the above critiques and current needs, this review summarizes different protective strategies based on bioactive compounds present in plants. Various bioactive compounds from medicinal plants with neuroprotective capacities are discussed with relevant examples. Many in vitro studies on clinical trials have shown promising outcomes using plant-based bioactive compounds against neurological disorders.

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment.

Marine environments accommodating diverse assortments of life constitute a great pool of differentiated natural resources. The cumulative need to remedy unpropitious effects of anthropogenic activities on estuaries and coastal marine ecosystems has propelled the development of effective bioremediation strategies. Marine bacteria producing biosurfactants are promising agents for bio-remediating oil pollution in marine environments, making them prospective candidates for enhancing oil recovery. Molecular omics technologies are considered an emerging field of research in ecological and diversity assessment owing to their utility in environmental surveillance and bioremediation of polluted sites. A thorough literature review was undertaken to understand the applicability of different omic techniques used for bioremediation assessment using marine bacteria. This review further establishes that for bioremediation of environmental pollutants (i.e. heavy metals, hydrocarbons, xenobiotic and numerous recalcitrant compounds), organisms isolated from marine environments can be better used for their removal. The literature survey shows that omics approaches can provide exemplary knowledge about microbial communities and their role in the bioremediation of environmental pollutants. This review centres on applications of marine bacteria in enhanced bioremediation, using the omics approaches that can be a vital biological contrivance in environmental monitoring to tackle environmental degradation. The paper aims to identify the gaps in investigations involving marine bacteria to help researchers, ecologists and decision-makers to develop a holistic understanding regarding their utility in bioremediation assessment.

Evaluation of cytotoxicity and genotoxicity effects of refractory pollutants of untreated and biomethanated distillery effluent using Allium cepa.

Environmental pollution caused by the discharge of raw and partly treated distillery effluent has become a serious and threatening problem due to its high pollution load. The aim of the present study was to assess the physicochemical load in alcohol distillery effluent before and after biomethanation treatment and the cyto- and genotoxicity effects of refractory pollutants emanated in raw/untreated and biomethanated distillery effluent on the ultrastructural and biochemical responses of Allium cepa root tip cells. Physicochemical analysis revealed high biochemical oxygen demand (BOD: 47840-36651 mg L-1), chemical oxygen demand (COD: 93452-84500 mg L-1) and total dissolved solids (TDS: 64251-74652 mg L-1) in raw and biomethanated effluent along with metal(loid)s (Fe: 456.152-346.26; Zn: 1.654-1.465; Cu: 0.648-0.562; Ni: 1.012-0.951, and Pb: 0.264 mg L-1) which were beyond the safe discharge values prescribed by the environmental regulatory agencies. The UV-Visible and Fourier transform infrared spectrophotometry analyses confirmed the high levels of organic, inorganic, and mixed contaminants discharged in raw and biomethanated distillery effluents. Furthermore, GC-MS analysis characterised chemical contaminants, such as hexadecanoic acid, butanedioic acid, bis(trimethylsilyl) ester; hexadecane, 2,6,11,15-tetramethyl, stigmasterol, and ß-sitosterol trimethylsilyl ether that have been reported as androgenic-mutagenic, and endocrine disrupting chemicals by the United States Environmental Protection Agency (U.S. EPA). The cytotoxicity measured by A. cepa showed dose depended inhibition root growth inhibition and simultaneous reduction in mitotic index in tested effluents. The chromosomal aberrations studies resulted in laggard chromosomes, sticky chromosomes, vagrant chromosomes, chromosome loss, c-mitosis, chromosome bridge, abnormal metaphase, and disturbed anaphase as found in a dose-dependent manner. Furthermore, dose-dependent enhancement in the levels of malondialdehyde, hydrogen peroxide, and antioxidative enzymes, such as superoxide dismutase, ascorbate peroxidase, and catalase were found to be higher in raw effluents treated root cells compared to biomethanated distillery effluent. Analysis of ultrastructural changes in root tip cells by TEM analysis revealed dramatic changes in the morphology of cell organelles and accumulation of metallic elements in and on the surface tissues. The results concluded that the discharged distillery effluents retained certain toxic pollutants which imposed cytotoxic and genotoxic hazards to A. cepa. Thus, for the sake of environmental protection, the raw as well as the disposed biomethanated effluent must be efficiently treated before its dumping into the terrestrial ecosystem.

Detection and characterization of refractory organic and inorganic pollutants discharged in biomethanated distillery effluent and their phytotoxicity, cytotoxicity, and genotoxicity assessment using Phaseolus aureus L. and Allium cepa L.

The color effluent discharged by alcohol distilleries comprises very high pollution loads due to the plethora of refractory chemicals even after anaerobic treatment and causing adverse effects to the environment. The present study aimed to examine the phytotoxic, cytotoxic, and genotoxic potential of the identified refractory organic and inorganic pollutants discharged in bio-methanated distillery effluent (BMDE). Physico-chemical analyses revealed that BMDE retains high BOD, COD, TDS along with heavy metals like Fe (572.64 mg L-1), Mn (4.269 mg L-1), Cd (1.631 mg L-1), Zn (2.547 mg L-1), Pb (1.262 mg L-1), (Cr 1.257 mg L-1), and Ni (0.781 mg L-1) beyond the permissible limits for effluent discharge. GC-MS analysis revelaed the presence of hexadecanoic acid, TMS ester; octadecanoic acid, TMS ester; 2,3 bis[(TMS)oxy]propyl ester; stigmasterol TMS ether; ß-sitosterol TMS ester; hexacosanoic acid; and tetradecanoic acid, TMS ester as major refractory organic pollutants, which are listed as potential endocrine disruptor chemicals (EDCs) as per USEPA. Furthermore, phytotoxicity assessment with Phaseolus aureus L. showed the toxic nature of BMDE as it inhibited various seedling growth parameters, seed germination, and suppression of α-amylase activity in seed germination experiment. Moreover, genotoxicity and cytotoxicity evaluation of the discharged BMDE evidenced in root-tip meristematic cells of Allium cepa L. where chromosomal aberration such as disturbed metaphase, c-mitosis, laggard chromosomes, sticky chromosomes, prolonged prophase, polyploid cells, and apoptotic bodies etc. were observed. Thus, this study's results suggested that BMDE discharged without adequate treatment poses potential risk to environment and may cause a variety of serious health threats in living beings upon exposure.

A unique polysaccharide containing 3-O-methylarabinose and 3-O-methylgalactose from Tinospora sinensis.

Tinospora sinensis (Lour.) Merrill is of great therapeutic significance in Indian traditional medicine. Crude polysaccharides were isolated from methanol pre-extracted stems of dried material by successive extractions with cold water, hot water and NaOH (0.25 mol/L) in 0.98, 0.55 and 0.70 % yields respectively. Cold water soluble polysaccharides (CWSP) were purified and fractionated by ion exchange chromatography on DEAE-Sephacel. Neutral polysaccharides were further fractionated on Sepharose CL6B to yield three fractions TW1, TW2, TW3. The study further focuses on structural elucidation of TW1. TW1 was obtained in 0.8 % yield relative to CWSP, with MW of 1.6 × 105 Da. It was composed of 3-O-methyl-arabinose, 3-O-methyl-galactose and galactose in molar ratio of 1.0:6.3:0.9 respectively. Based on per-deuteromethylation, NMR and ESI-MS analyses, TW1 was composed of 1,4-linked 3-O-methyl-ß-d-galactopyranose and ß-d-galactopyranose backbone with branching at O-6 of 3-O-methyl-ß-d-galactosyl residues by 1,5-linked 3-O-methyl-α-l-arabinofuranoside chains. 3-O-methyl-arabinose and 3-O-methyl-galactose have first ever been reported in any polysaccharide and Tinospora genus, respectively.

Affected energy metabolism under manganese stress governs cellular toxicity.

Excessive manganese exposure is toxic, but a comprehensive biochemical picture of this assault is poorly understood. Whether oxidative stress or reduced energy metabolism under manganese exposure causes toxicity is still a debate. To address this, we chose Δmnt P Escherichia coli, a highly manganese-sensitive strain, in this study. Combining microarray, proteomics, and biochemical analyses, we show that the chronic manganese exposure rewires diverse regulatory and metabolic pathways. Manganese stress affects protein and other macromolecular stability, and envelope biogenesis. Most importantly, manganese exposure disrupts both iron-sulfur cluster and heme-enzyme biogenesis by depleting cellular iron level. Therefore, the compromised function of the iron-dependent enzymes in the tricarboxylic acid cycle, and electron transport chain impede ATP synthesis, leading to severe energy deficiency. Manganese stress also evokes reactive oxygen species, inducing oxidative stress. However, suppressing oxidative stress does not improve oxidative phosphorylation and cell growth. On the contrary, iron supplementation resumed cell growth stimulating oxidative phosphorylation. Therefore, we hypothesize that affected energy metabolism is the primal cause of manganese toxicity.

Monosaccharide composition of acidic gum exudates from Indian Acacia tortilis ssp. raddiana (Savi) Brenan.

Acacia tortilis ssp. raddiana (Savi) Brenan commonly known as Israeli Babool has contributed immensely for sand dunes management in Indian desert leading to wind erosion control and increased biological productivity. The species is extensively used in traditional medicine system for a number of therapeutic applications and as nutraceutical. The polysaccharide was isolated in 43.6% yield from gum exudates. The monosaccharides, L-arabinose, D-galactose D-glucose, L-rhamnose and D-mannose were determined in molar ratio of 78.1%, 18.64%, 0.60%, 1.71% and 0.74% respectively. The molar ratio of uronic acids was studied using diverse spectrophotometric methods and compared with GLC. The content of D-galacturonic acid and D-glucuronic was determined as 3.88% and 4.35% respectively by GLC. The results were compared with the spectrophotometric methods. The results using DMP as chromogenic reagent are closer to that obtained by GLC. Structural analysis of the polysaccharide may provide scientific basis for nutraceutical, pharmaceutical and biological applications of gum exudates from A. tortilis, which is extensively planted in India.

Quantitative transporter proteomics by liquid chromatography with tandem mass spectrometry: addressing methodologic issues of plasma membrane isolation and expression-activity relationship.

To predict transporter-mediated drug disposition using physiologically based pharmacokinetic models, one approach is to measure transport activity and relate it to protein expression levels in cell lines (overexpressing the transporter) and then scale these to via in vitro to in vivo extrapolation (IVIVE). This approach makes two major assumptions. First, that the expression of the transporter is predominantly in the plasma membrane. Second, that there is a linear correlation between expression level and activity of the transporter protein. The present study was conducted to test these two assumptions. We evaluated two commercially available kits that claimed to separate plasma membrane from other cell membranes. The Qiagen Qproteome kit yielded very little protein in the fraction purported to be the plasma membrane. The Abcam Phase Separation kit enriched the plasma membrane but did not separate it from other intracellular membranes. For the Abcam method, the expression level of organic anion-transporting polypeptides (OATP) 1B1/2B1 and breast cancer resistance protein (BCRP) proteins in all subcellular fractions isolated from cells or human liver tissue tracked that of Na⁺-K⁺ ATPase. Assuming that Na⁺-K⁺ ATPase is predominantly located in the plasma membrane, these data suggest that the transporters measured are also primarily located in the plasma membrane. Using short hairpin RNA, we created clones of cell lines with varying degrees of OATP1B1 or BCRP expression level. In these clones, transport activity of OATP1B1 or BCRP was highly correlated with protein expression level (r² > 0.9). These data support the use of transporter expression level data and activity data from transporter overexpressing cell lines for IVIVE of transporter-mediated disposition of drugs.

Workplace spirituality as a moderator in relation between stress and health: An exploratory empirical assessment.

The present study explores the role of workplace spirituality in moderating the relationship between occupational stress and the health of managerial personnel in India. A sample of 150 managers working in different public and private organizations was used to measure workplace spirituality, occupational stress, and health using the Spirituality at Work scale, the Occupational Stress Index and the 28-item General Health Questionnaire, respectively. The findings reveal that workplace spirituality moderates the negative relationship of stress and health. The study also found that stress has a negative impact on health while workplace spirituality positively correlated with health. The findings also support the practical importance of spirituality in the workplace for improving health conditions by providing a healthy atmosphere and meaningful work for employees. This exploratory study encourages future research to understand the role of spirituality in the workplace.

Biosurfactant stabilized anticancer biomolecule-loaded poly (D,L-lactide) nanoparticles.

PLA nanoparticles (NPs) were prepared via green route using turmeric (Curcuma longa) extract (TE) as biostabiliser/biosurfactant. Of the 29 formulations, two formulations of TE synthesized PLA NPs were evaluated for encapsulation and controlled release of well known antioxidant and less bioavailable molecules curcumin and quercetin. Size of curcumin loaded PLA NPs synthesized using 0.8 mg/ml PLA (C-En-D) and 0.1 mg/ml PLA (C-En-P) were 203±77 and 110±44 nm, respectively. However, quercetin loaded PLA NPs synthesized at 0.8 mg/ml (Q-En-D) and 0.1mg/ml (Q-En-P) PLA concentrations were 170±95 and 102±31 nm, respectively. The encapsulation efficiency of curcumin loaded PLA (C-En-D and C-En-P) NPs as well as quercetin loaded PLA (Q-En-D and Q-En-P) NPs was found ∼95%. In vitro release study of C-En-D, C-En-P, Q-En-D and Q-En-P NPs showed initial burst release followed by slow and sustained release. C-En-D NPs and Q-En-D NPs showing maximum in vitro release (∼100%) were evaluated for cytotoxicity. Blank PLA NPs, C-En-D and Q-En-D NPs were found to be safe against normal human leukocytes up to 2 mg/ml dose. Both C-En-D and Q-En-D NPs showed anticancer activity against A549 cell line. But Q-En-D NPs showed better anticancer activity than C-En-D NPS on A549 cells. While blank PLA NPs did not possess anticancer activity. TE extract stabilized PLA NPs were non-toxic, biocompatible and safe to normal human leukocytes. Such technology will be better, effective and safer in use for anticancer as well as other biological application.

Aegle marmelos fruit pectin for food and pharmaceuticals: Physico-chemical, rheological and functional performance.

Pectin is used in a number of foods as a gelling agent, thickener, texturizer, emulsifier and stabilizer. Bael fruit, obtained from Aegle marmelos, is a rich source of pectin. Bael fruit pectin (BFP) was extracted from ripe Bael fruits. The process yielded 15% (w/w) pure BFP. The swelling index decreased in the following order: water>pH 7.4>pH 6.8>pH 1.2>HCl (0.1N). Galacturonic acid content of 87.8%, degree of esterification of 47.2%, 17.3% methoxy groups, 0.29% acetyl groups and equivalent weight of 1209.5, indicate it to be a good gelling agent and easily amenable to derivatization. BFP exhibited a significant concentration-dependent prolongation of prothrombin time. The absence of hemagglutinating activity and antinutritional factors coupled with the activity to confer better emulsion capacity, stability and antimicrobial activity gives BFP a clear edge over commercial citrus pectin (CP) for exploitation as an additive in food and pharmaceuticals.

Molecular weight determination and correlation analysis of Dalbergia sissoo polysaccharide with constituent oligosaccharides.

INTRODUCTION: Mucilaginous polysaccharide extracted from Dalbergia sissoo Roxb. leaves has a number of medicinal applications. Molecular weight studies and correlation analysis of the structure of polysaccharide with oligosaccharides can be helpful for further utilisation, modification and structure-activity relationship for biological applications. OBJECTIVE: To determine molecular weight of medicinally important polysaccharide. To establish an unequivocal correlation of the polysaccharide monosugars with constituting oligosaccharides and glucuronic acid content based on gas-liquid chromatography (GLC) with the spectrophotometric method. METHODOLOGY: Complete and partial hydrolytic studies of pure polysaccharide yielded constituting monosugars and oligosaccharides. The ratio of sugars in polysaccharide and oligosaccharides was studied by preparation of alditol acetates and analysed using GLC. The uronic acid content was studied by GLC analysis and spectrophotometry. Molecular weight of the polysaccharide was determined using the viscometric method. RESULTS: Dalbergia sissoo leaves yielded 14.0% pure polysaccharide, containing 15.7% of glucuronic acid. Complete hydrolysis and GLC analysis of alditol acetate derivatives of reduced and unreduced monosugars indicated the presence of L-rhamnose, D-glucuronic acid, D-galactose and D-glucose in 1.00:1.00:2.00:2.33 molar ratios. Partial hydrolysis followed by monosugar analysis of oligosaccharides established the monosugar ratio in complete agreement with polysaccharide, thereby corroborating the sugar ratio. Similar uronic acid content was obtained by GLC and spectrophotometry. The polysaccharide had an average molecular weight of 1.5 × 105 Da. CONCLUSION: The study has established an obvious correlation of the structure of polysaccharide with oligosaccharides, leading to unambiguous identification of monosaccharides, which normally is not studied conclusively while reporting the polysaccharide structure. The molecular weight of the polysaccharide was determined.

An insight into the properties of Aegle marmelos pectin-chitosan cross-linked films.

Pectin of Aegle marmelos (AP) ripe fruits processed in equal proportion with chitosan (CH) formed films that exhibited minimum swelling index and volume index on exposure to buffers of acidic and alkaline pH. Highest contact angle and spreading coefficient coupled with lowest work of adhesion in all buffers for this film suggested availability of limited number of functional groups for interaction with water molecules due to optimum cross-linking between -NH(3)(+) groups of CH and -COO(-) groups of AP. This contention was substantiated by the presence of almost negligible charge on this film. The endothermic transition ΔH characteristic of -NH(3)(+)-COO(-) cross-linking between groups in this film was observed to decrease by only 1% after its sequential exposure to pH 1.2 (3 h) and pH 7.4 (6 h). Furthermore, the absence of pores or erosion in the scanning electron photomicrograph suggested the versatility of this film due to its resistance to acidic and alkaline pH.

A self-guided relaxation module for telepsychiatric services: development, usefulness, and feasibility.

OBJECTIVE: A modified form of the standard autogenic relaxation exercise was developed as part of a telepsychiatry project, which aims to deliver mental healthcare to remote areas through the net, and through the agency of non-specialists. This study describes the development and evaluation of a brief and simpler form of a relaxation technique, the Self-Guided Relaxation (SGR). METHOD: SGR is a systematic technique to focus on muscle groups and feel the heaviness and relaxation through suggestions to induce relaxation in the body. The SGR was taught to 37 patients with different psychiatric disorders over two sessions a week apart. Measures of change used were scores on the Hamilton Anxiety Rating Scale (HAM-A) and a visual analog scale. Its usefulness and feasibility was examined over a follow-up period of one month. Additionally a group of non-specialists was trained in SGR supplemented by audiovisual aids and printed instructions. These therapists rated the ease in learning and administering SGR in patients. RESULTS: There was a consistent reduction in the HAM-A scores over the follow-up period among patients. Improvements were also noted in positive experiences, adherence, depth of relaxation achieved, perceived benefit, and patient satisfaction. Majority of the patients, relatives, and therapists reported that they could administer or practice SGR after having learned the technique in a single session lasting about 30 minutes. The use of audio-video aids and manual was helpful in making the process of learning easy. CONCLUSIONS: Preliminary findings of usefulness, feasibility, and acceptance of the SGR were encouraging. These findings pave way for larger, randomized controlled study and for testing applicability of the SGR as a net-based psychological intervention.

Plant extract synthesized PLA nanoparticles for controlled and sustained release of quercetin: a green approach.

BACKGROUND: Green synthesis of metallic nanoparticles (NPs) has been extensively carried out by using plant extracts (PEs) which have property of stabilizers/emulsifiers. To our knowledge, there is no comprehensive study on applying a green approach using PEs for fabrication of biodegradable PLA NPs. Conventional methods rely on molecules like polyvinyl alcohol, polyethylene glycol, D-alpha-tocopheryl poly(ethylene glycol 1000) succinate as stabilizers/emulsifiers for the synthesis of such biodegradable NPs which are known to be toxic. So, there is urgent need to look for stabilizers which are biogenic and non-toxic. The present study investigated use of PEs as stabilizers/emulsifiers for the fabrication of stable PLA NPs. Synthesized PLA NPs through this green process were explored for controlled release of the well known antioxidant molecule quercetin. METHODOLOGY/PRINCIPAL FINDINGS: Stable PLA NPs were synthesized using leaf extracts of medicinally important plants like Syzygium cumini (1), Bauhinia variegata (2), Cedrus deodara (3), Lonicera japonica (4) and Eleaocarpus sphaericus (5). Small and uniformly distributed NPs in the size range 70±30 nm to 143±36 nm were formed with these PEs. To explore such NPs for drugs/ small molecules delivery, we have successfully encapsulated quercetin a lipophilic molecule on a most uniformly distributed PLA-4 NPs synthesized using Lonicera japonica leaf extract. Quercetin loaded PLA-4 NPs were observed for slow and sustained release of quercetin molecule. CONCLUSIONS: This green approach based on PEs mediated synthesis of stable PLA NPs pave the way for encapsulating drug/small molecules, nutraceuticals and other bioactive ingredients for safer cellular uptake, biodistribution and targeted delivery. Hence, such PEs synthesized PLA NPs would be useful to enhance the therapeutic efficacy of encapsulated small molecules/drugs. Furthermore, different types of plants can be explored for the synthesis of PLA as well as other polymeric NPs of smaller size.