RESUMEN
OBJECTIVE: To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity. METHODS: The crude polysaccharides were extracted by hot water and precipitated with ethanol, then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography. After determined by chemicophysical analysis, the polished polysaccharides were selenium treated and bio-assayed by MT and CPE methods. RESULTS: The crude extraction from Caulerpa taxifolia was a kind of sulfated polysaccharides in which the average content of polysaccharides and sulfate were 27. 9% and 11.5%, respectively. The recovery rate of the polished polysaccharides was 66.3% after the 2-column purification. The IC50 and TI of the purified polysaccharide SCpl1 were 2.2 mg/mL and 3057.0, respectively. After treated with selenium, the average diameter of the nanoparticle was 28.6 nm, and its bioactivity and TI index were enhanced significantly although with a higher cytotoxicity. CONCLUSION: The polysaccharides from Caulerpa taxifolia and its selenium particles was a kind of bioactive substance for anti-virus drug-candidate development.
Asunto(s)
Caulerpa/química , Enterovirus Humano B/efectos de los fármacos , Polisacáridos , Selenio/química , Tecnología Farmacéutica/métodos , Antivirales/aislamiento & purificación , Antivirales/farmacología , Células HeLa , Humanos , Nanoestructuras , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Selenio/farmacologíaRESUMEN
OBJECTIVE: To determine the activity of anti-Herpes simplex II virus (HSV-2) of amylose extracted from Grateloupia filicina. METHODS: Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex II virus was observed on cell level in three aspects: the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2. RESULTS: Grateloupia filicina had prominence anti-Herpes simplex II virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.80 microg/ml. CONCLUSION: It suggest that the antivirus activity happen in the stage of HSV-2 binding, adsorption and ingression with Vero cell.
Asunto(s)
Amilosa/aislamiento & purificación , Amilosa/farmacología , Antivirales/farmacología , Herpesvirus Humano 2/efectos de los fármacos , Rhodophyta/química , Animales , Células Cultivadas , Chlorocebus aethiops , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Etanol , Plantas Medicinales/química , Células Vero , Replicación Viral/efectos de los fármacos , AguaRESUMEN
To isolate polysaccharides with hot water from the caudex of Undaria pinnatifida, and precipitate with ethanol. The protein in polysaccharides was removed by sevage way. DEAE-52 and Sephadex G-200 column chromatography were used to isolate and purification polysaccharides, three purified polysaccharides F2, F3 and F4 were obtained. It was identified that they were homogeneity. The ultraviolet spectrum showed there was no proteins and nucleic acids in F2, F3 and F4. Through thin-layer chromatography analysis, F2, F3 and F4 were mainly composed of Gal, Fuc, Man and Glu acid. F2 also contained Glu and Rha.