RESUMEN
Functional core/shell particles are highly sought after in analytical chemistry, especially in methods suitable for single-particle analysis such as flow cytometry because they allow for facile multiplexed detection of several analytes in a single run. Aiming to develop a powerful bead platform of which the core particle can be doped in a straightforward manner while the shell offers the highest possible sensitivity when functionalized with (bio)chemical binders, polystyrene particles were coated with different kinds of mesoporous silica shells in a convergent growth approach. Mesoporous shells allow us to obtain distinctly higher surface areas in comparison with conventional nonporous shells. While assessing the potential of narrow- as well as wide-pore silicas such as Mobil composition of matter no. 41 (MCM-41) and Santa Barbara amorphous material no. 15 (SBA-15), especially the synthesis of the latter shells that are much more suitable for biomolecule anchoring was optimized by altering the pH and both, the amount and type of the mediator salt. Our studies showed that the best performing material resulted from a synthesis using neutral conditions and MgSO4 as an ionic mediator. The analytical potential of the particles was investigated in flow cytometric DNA assays after their respective functionalization for individual and multiplexed detection of short oligonucleotide strands. These experiments revealed that a two-step modification of the silica surface with amino silane and succinic anhydride prior to coupling of an amino-terminated capture DNA (c-DNA) strand is superior to coupling carboxylic acid-terminated c-DNA to aminated core/shell particles, yielding limits of detection (LOD) down to 5 pM for a hybridization assay, using labeled complementary single-stranded target DNA (t-DNA) 15mers. The potential of the use of the particles in multiplexed analysis was shown with the aid of dye-doped core particles carrying a respective SBA-15 shell. Characteristic genomic sequences of human papillomaviruses (HPV) were chosen as the t-DNA analytes here, since their high relevance as carcinogens and the high number of different pathogens is a relevant model case. The title particles showed a promising performance and allowed us to unequivocally detect the different high- and low-risk HPV types in a single experimental run.
Asunto(s)
ADN Viral/análisis , Citometría de Flujo/métodos , Microplásticos/química , Poliestirenos/química , Dióxido de Silicio/química , Alphapapillomavirus/química , Compuestos de Boro/química , ADN de Cadena Simple/análisis , ADN de Cadena Simple/genética , ADN Viral/genética , Fluoresceínas/química , Colorantes Fluorescentes/química , Límite de Detección , Hibridación de Ácido Nucleico , Oligonucleótidos/química , Oligonucleótidos/genética , PorosidadRESUMEN
Many polymer decorated/modified 2D nanomaterials have been developed as enhanced drug delivery systems and photothermal theranostic nanoagents. However, few reports describe the use of these novel nanomaterials as nanoplatforms for biomolecule sensing. Herein, we used calcium-cation-doped polydopamine-modified (PDA-modified) 2D black phosphorus (BP) nanosheets (BP@PDA) as a sensing nanoplatform for the detection of nucleic acids and proteins in complex biological samples. Fluorescent-dye-labeled single-strand DNA aptamer/probes are adsorbed by the Ca2+-doped BP@PDA mediated by calcium-cation coordination. The PDA coating enhances the stability of the inner BP, provides binding sites to DNA nucleobases, and quenches fluorescence. Without any chemical conjugation, this sensing nanoplatform selectively and specifically detects protein (human thrombin, linear range: 10-25 nM, detection limit: 0.02 nM), single-strand DNA (linear range: 1-10 nM, detection limit: 0.52 nM) in 1% serum diluted samples, and senses intracellular mRNAs (C-myc, and actin) in living cells. The nanoplatform exhibits the advantages of both the 2D nanomaterial (BP) and the coating polymer (PDA), naturally enters living cells unaided by transfection agents, resists enzymatic lysis and shows high biocompatibility. This nanoplatform design contributes towards future biomolecule analytical method development based on polymer decorated/modified 2D nanomaterials.
Asunto(s)
Calcio/química , Indoles/química , Nanoestructuras/química , Fósforo/química , Polímeros/química , Espectrometría de Fluorescencia/métodos , Trombina/análisis , Cationes/química , Supervivencia Celular/efectos de los fármacos , Sondas de ADN/química , Sondas de ADN/metabolismo , ADN de Cadena Simple/análisis , ADN de Cadena Simple/química , Colorantes Fluorescentes/química , Células Hep G2 , Humanos , Límite de Detección , Microscopía Confocal/métodos , Nanoestructuras/toxicidad , Proteínas Proto-Oncogénicas c-myc/genética , ARN Mensajero/análisisRESUMEN
The aim of this study is to develop a methodology in which Surface Plasmon Resonance (SPR), Ellipsometer (EM) and Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry (MALDI-MS) will be used together for detection of single-strand oligodeoxynucleotides (ssODNs) targets. A selected target-ssODNs, and its complementary, the probe-ssODNs carrying a -SH end group, a spacer arm (HS-(CH2)6-(T)15, and a non-complementary ssODNs were used. Silicone based stamps with 16 regions were prepared and used for micro-contact printing (µCP) of the probe-ssODNs on the gold coated surfaces homogeneously. A modulator-spacer molecule (6-mercapto-1-hexanol) was co-immobilized to control surface probe density, to orientate the probe-ssODNs, and to eliminate the nonspecific interactions. SPR was used successfully to follow the hybridization of the target-ssODNs with the immobilized probe-ssODNs on the platform surfaces. Complete hybridizations were achieved in 100 min. It was obtained that there was a linear relationship between relative change in delta and target concentration below 1 µm. Using imaging version of ellipsometer (IEM) allowed imaging of the surfaces and supported extra datum for the SPR results. After a very simple dehybridization protocol, MALDI-MS analysis allowed detection of the target-ssODNs hybridized on the sensor/array platforms.
Asunto(s)
Técnicas Biosensibles , ADN de Cadena Simple/análisis , Hibridación de Ácido Nucleico , Resonancia por Plasmón de Superficie , Técnicas Biosensibles/instrumentación , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/instrumentación , Resonancia por Plasmón de Superficie/instrumentaciónRESUMEN
A novel enhanced photoelectrochemical (PEC) DNA biosensor, based on a compact heterojunction g-C3N4/MoS2 and co-sensitization effect with CdSe quantum dots (QDs), was first proposed for simple and accurate analysis of a short ssDNA. In this work, the g-C3N4/MoS2 was successfully synthesized and used as the electrode matrix material to construct PEC biosensor. 2D/2D heterojunction was formed between g-C3N4 and MoS2, which could promote the separation of photogenerated electron-hole pairs resulting in an enhanced photocurrent. In the presence of target DNA, CdSe QDs labeled reporter DNA was complementary pairing with target DNA which was specific recognized by capture DNA loading on self-assembled CdS QDs film, leading to close contact between CdSe QDs and g-C3N4/MoS2 modified electrode surface, thereby resulting in the enhanced photocurrent intensity due to the co-sensitization effect. Under the optimal operating conditions, the photoelectrochemical biosensor demonstrated favorable accuracy and could respond to 0.32 pM (S/Nâ¯=â¯3) with a linear concentration range from 1.0 pM to 2.0⯵M. Moreover, the proposed PEC DNA biosensor exhibits high sensitivity, excellent specificity, acceptable reproducibility and accuracy, showing a promising potential in DNA bioanalysis and other relative fields.
Asunto(s)
Técnicas Biosensibles/métodos , ADN de Cadena Simple/análisis , Técnicas Electroquímicas/métodos , Fotoquímica/métodos , Puntos Cuánticos/química , Compuestos de Cadmio/química , Compuestos de Cadmio/efectos de la radiación , ADN de Cadena Simple/genética , Disulfuros/química , Electrodos , Luz , Límite de Detección , Molibdeno/química , Nitrilos/química , Hibridación de Ácido Nucleico , Puntos Cuánticos/efectos de la radiación , Compuestos de Selenio/química , Compuestos de Selenio/efectos de la radiaciónRESUMEN
Combined separation and detection of biomolecules has the potential to speed up and improve the sensitivity of disease detection, environmental testing, and biomolecular analysis. In this work, we synthesized magnetic particles coated with spiky nanostructured gold shells and used them to magnetically separate out and detect oligonucleotides using SERS. The distance dependence of the SERS signal was then harnessed to detect DNA hybridization using a Raman label bound to a hairpin probe. The distance of the Raman label from the surface increased upon complementary DNA hybridization, leading to a decrease in signal intensity. This work demonstrates the use of the particles for combined separation and detection of oligonucleotides without the use of an extrinsic tag or secondary hybridization step.
Asunto(s)
Técnicas Biosensibles , ADN de Cadena Simple/análisis , ADN/análisis , Nanopartículas de Magnetita/química , Nanoestructuras/química , Espectrometría Raman/normas , Cloruros/química , Sondas de ADN/síntesis química , Sondas de ADN/química , Compuestos Férricos/química , Oro/química , Humanos , Secuencias Invertidas Repetidas , Nanopartículas de Magnetita/ultraestructura , Nanoestructuras/ultraestructura , Hibridación de Ácido Nucleico , Oligonucleótidos/química , Sensibilidad y Especificidad , Dióxido de Silicio/química , SolucionesRESUMEN
Since its discovery in the 1970s, surface-enhanced Raman scattering (SERS) has been primarily associated with substrates composed of nanostructured noble metals. Here we investigate chemically synthesized nanocrystal aggregates of aluminum, an inexpensive, highly abundant, and sustainable metal, as SERS substrates. Al nanocrystal aggregates are capable of substantial near-infrared SERS enhancements, similar to Au nanoparticles. The intrinsic nanoscale surface oxide of Al nanocrystals supports molecule-substrate interactions that differ dramatically from noble metal substrates. The preferential affinity of the single-stranded DNA (ssDNA) phosphate backbone for the Al oxide surface preserves both the spectral features and nucleic acid cross sections relative to conventional Raman spectroscopy, enabling quantitative ssDNA detection and analysis.
Asunto(s)
Aluminio/química , ADN de Cadena Simple/análisis , Nanopartículas del Metal/química , Óxidos/química , Óxido de Aluminio/química , Secuencia de Bases , Medios de Contraste/química , Tamaño de la Partícula , Fenómenos Físicos , Espectrometría Raman , Propiedades de SuperficieRESUMEN
Despite the potential for nanopores to be a platform for high-bandwidth study of single-molecule systems, ionic current measurements through nanopores have been limited in their temporal resolution by noise arising from poorly optimized measurement electronics and large parasitic capacitances in the nanopore membranes. Here, we present a complementary metal-oxide-semiconductor (CMOS) nanopore (CNP) amplifier capable of low noise recordings at an unprecedented 10 MHz bandwidth. When integrated with state-of-the-art solid-state nanopores in silicon nitride membranes, we achieve an SNR of greater than 10 for ssDNA translocations at a measurement bandwidth of 5 MHz, which represents the fastest ion current recordings through nanopores reported to date. We observe transient features in ssDNA translocation events that are as short as 200 ns, which are hidden even at bandwidths as high as 1 MHz. These features offer further insights into the translocation kinetics of molecules entering and exiting the pore. This platform highlights the advantages of high-bandwidth translocation measurements made possible by integrating nanopores and custom-designed electronics.
Asunto(s)
ADN de Cadena Simple/análisis , Nanoporos , Semiconductores , NanotecnologíaRESUMEN
This paper concerns the development of genosensors based on redox-active monolayers incorporating (dipyrromethene)2Cu(II) and (dipyrromethene)2Co(II) complexes formed step by step on a gold electrode surface. They were applied for electrochemical determination of oligonucleotide sequences related to avian influenza virus (AIV) type H5N1. A 20-mer probe (NH2-NC3) was covalently attached to the gold electrode surface via a reaction performed in the presence of ethyl(dimethylaminopropyl)carbodiimide / N-hydroxysuccinimide (EDC/NHS) between the amine group present in the probe and carboxylic groups present on the surface of the redox-active layer. Each modification step has been controlled with Osteryoung square-wave voltammetry. The genosensor incorporating the (dipyrromethene)2Cu(II) complex was able to detect a fully complementary single-stranded DNA target with a detection limit of 1.39 pM. A linear dynamic range was observed from 1 to 10 pM. This genosensor displays good discrimination between three single-stranded DNA targets studied: fully complementary, partially complementary (with only six complementary bases), and totally noncomplementary to the probe. When the (dipyrromethene)2Co(II) complex was applied, a detection limit of 1.28 pM for the fully complementary target was obtained. However, this genosensor was not able to discriminate partially complementary and totally noncomplementary oligonucleotide sequences to the probe. Electrochemical measurements, using both types of genosensors in the presence of different supporting electrolytes, were performed in order to elaborate a new mechanism of analytical signal generation based on an ion barrier "switch-off" system.
Asunto(s)
Cobalto/química , Cobre/química , ADN de Cadena Simple/análisis , Técnicas Electroquímicas/métodos , Subtipo H5N1 del Virus de la Influenza A/aislamiento & purificación , Gripe Aviar/virología , Porfobilinógeno/análogos & derivados , Animales , Técnicas Biosensibles/métodos , Aves , Complejos de Coordinación/química , ADN de Cadena Simple/genética , Electrodos , Oro/química , Subtipo H5N1 del Virus de la Influenza A/genética , Gripe Aviar/diagnóstico , Límite de Detección , Hibridación de Ácido Nucleico/métodos , Porfobilinógeno/químicaRESUMEN
Amplification-free (referring to enzymatic amplification step) detection methodologies are increasing in biosensor development due to the need of faster and simpler protocols. However, for maintaining sensitivity without this step, highly detectable molecules or very sensitive detection techniques are required. The nanostructuration of transducer surfaces with carbon nanotubes (CNTs), gold nanoparticles (AuNPs) or both in nanohybrid configurations has been employed in this work for DNA hybridisation sensing purposes. Methylene blue (MB), covalently attached to single stranded DNA, (ssDNA) was incubated with a complementary sequence immobilized on nanostructured screen-printed electrodes (AuSPEs). Although CNTs can increase notoriously the signal of the marker, adsorptive properties should also be considered when bioassays are performed because non-specific adsorption (NSA) phenomena are magnified. In this work, strategies for decreasing NSA were thoroughly evaluated for the detection of Mycoplasma pneumoniae (MP) on CNTs-nanostructured screen-printed electrodes. Among them, the employ of UV-radiation or long incubation times (72h) allowed obtaining higher signals for the complementary strand with respect to the non-complementary one. The use of CNTs/AuNPs nanohybrids, together with the use of streptavidin-biotin (ST-B) interaction allows the higher differentiation (with a 3.5 ratio) in the genosensing of M. pneumoniae.
Asunto(s)
Técnicas Biosensibles , ADN Bacteriano/análisis , ADN de Cadena Simple/análisis , Mycoplasma pneumoniae/genética , Biotina/química , ADN Bacteriano/química , ADN de Cadena Simple/química , Electrodos , Oro/química , Nanopartículas del Metal/química , Azul de Metileno/química , Nanotubos de Carbono , Hibridación de Ácido Nucleico , Estreptavidina/químicaRESUMEN
Manganese dioxide (MnO2) nanosheets have recently been demonstrated to be particularly attractive for fluorescent sensing and imaging; however, almost all MnO2 nanosheets-based fluorescent assays have been developed with emissive nanoparticles as the probes. In this study, we developed a novel strategy to use organic dyes, instead of emissive nanoparticles, as the probe to construct a platform for biosensing with excellent analytical properties. With 5-carboxyfluorescein (FAM) as a model organic dye, we firstly investigate the effect of MnO2 nanosheets on the fluorescence of FAM and find that the fluorescence intensity of FAM is considerably suppressed by MnO2 nanosheets based on the inner filter effect (IFE). To demonstrate that the MnO2 nanosheets-based fluorescence sensing platform can easily achieve a high selectivity with organic dyes as the probe, we use single-stranded DNA (ssDNA) oligonucleotide as a typical biorecognition unit, which is labeled with the FAM probe to form FAM-ssDNA. The fluorescent intensity of FAM-ssDNA is first suppressed by MnO2 nanosheets through the combination of IFE and Förster resonant energy transfer (FRET), and then recovered with subsequent hybridization with the complementary DNA oligonucleotide. To demonstrate the potential applications of the MnO2 nanosheets-based fluorescence sensing platform with organic dyes as the probes, we developed methods for simple but effective microRNA and thrombin assays. With the platform demonstrated here, the limits of detection for miR124a and thrombin are 0.8 nM and 11 nM, respectively. Moreover, the fluorescent sensing assay for thrombin exhibits high selectivity. This study essentially demonstrates a new 2D nanostructure-based fluorescent sensing platform that is robust, technically simple, and easily manipulated to achieve high selectivity and sensitivity for practical applications.
Asunto(s)
Fluoresceínas/química , Colorantes Fluorescentes/química , Compuestos de Manganeso/química , Nanoestructuras/química , Óxidos/química , Espectrometría de Fluorescencia/métodos , Secuencia de Bases , ADN de Cadena Simple/análisis , ADN de Cadena Simple/química , ADN de Cadena Simple/genéticaRESUMEN
Isolating a particular strand of DNA from a double stranded DNA duplex is an important step in aptamer generation as well as many other biotechnology applications. Here we describe a microfluidic, flow-through, dialysis device for isolating single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA). The device consists of two channels fabricated in polydimethylsiloxane (PDMS) separated by a track etched polycarbonate membrane (800 nm pore size). To isolate ssDNA, dual-biotin labelled dsDNA was immobilized onto streptavidin-coated polystyrene beads. Alkaline treatment was used to denature dsDNA, releasing the non-biotinylated ssDNA. In the flow-through dialysis device the liberated ssDNA was able to cross the membrane and was collected in an outlet channel. The complementary sequence bound to the bead was unable to cross the membrane and was directed to a waste channel. The effect of NaOH concentration and flow rate on purity and yield were compared. >95% ssDNA purity was achieved at 25 mM NaOH. However, lower flow rates were necessary to achieve ssDNA yields approaching the 50% theoretical maximum of the concurrent-flow device. Under optimized conditions the microfluidic isolation achieved even higher purity ssDNA than analogous manual procedures.
Asunto(s)
ADN de Cadena Simple/aislamiento & purificación , Técnicas Analíticas Microfluídicas/métodos , ADN de Cadena Simple/análisis , Electroforesis Capilar/métodosRESUMEN
A light addressable potentiometric sensor (LAPS) is a kind of silicon based semiconductor sensor, and surface modification is a fundamental problem for its application in biological fields. Graphene oxide (GO) based biochemically activated LAPS were proposed, called GO-LAPS. The GO-LAPS were applied to monitoring single strand DNA (ssDNA) probe immobilization and its hybridization with complementary ssDNA molecules of different chain lengths (30, 21 and 14 base pairs, respectively). It was discovered that the curves of LAPS' currents versus analyte concentrations for ssDNA probe binding and the target ssDNA hybridization were different. Explanations were proposed based on the semiconductor's surface-electric-field-effect and the electrical properties of ssDNA molecule. Moreover, comparisons between GO-LAPS and LAPS without GO modification were carried out. Enhanced response currents of GO-LAPS were reported experimentally and analyzed theoretically based on X-ray photoelectron spectroscopy (XPS) of GO-LAPS. The limitation of target ssDNA monitoring was 1 pM to 10 nM, which suggested that this LAPS based platform could be developed as a sensitive means for short chain ssDNA detection.
Asunto(s)
ADN de Cadena Simple/análisis , Grafito/química , Luz , Óxidos/química , Potenciometría/instrumentación , Secuencia de Bases , ADN de Cadena Simple/química , ADN de Cadena Simple/genética , Electroquímica , Hibridación de Ácido Nucleico , SemiconductoresRESUMEN
Using variothermal polymer micro-injection molding, disposable arrays of eight polymer micro-cantilevers each 500 µm long, 100 µm wide and 25 µm thick were fabricated. The present study took advantage of an easy flow grade polypropylene. After gold coating for optical read-out and asymmetrical sensitization, the arrays were introduced into the Cantisens(®) Research system to perform mechanical and functional testing. We demonstrate that polypropylene cantilevers can be used as biosensors for medical purposes in the same manner as the established silicon ones to detect single-stranded DNA sequences and metal ions in real-time. A differential signal of 7 nm was detected for the hybridization of 1 µM complementary DNA sequences. For 100 nM copper ions the differential signal was found to be (36 ± 5) nm. Nano-mechanical sensing of medically relevant, nanometer-size species is essential for fast and efficient diagnosis.
Asunto(s)
Técnicas Biosensibles/métodos , Sistemas Microelectromecánicos , Polipropilenos/química , Polipropilenos/metabolismo , ADN de Cadena Simple/análisis , Humanos , Iones/análisis , Metales/análisis , Hibridación de Ácido NucleicoRESUMEN
The evaluation of novel electrochemically active label for electrochemical detection of DNA hybridization is presented. Metallacarborane units modified with iron, cobalt or chromium were investigated. The value of redox potential and relatively strong current signal facilitate usage of Fe-carborane as marker covalently attached to the ssDNA. In electrochemical genosensor the sequence complementary to UL55 gene was labeled and used as a target for biosensor device. Interactions were investigated using electrochemical and piezoelectric methods. Obtained results confirm usefulness of the designed label in electrochemical detection of DNA hybridization.
Asunto(s)
Boranos/química , Citomegalovirus/genética , ADN de Cadena Simple/análisis , Genes Virales , Hierro/química , Proteínas Virales/química , Cromo/química , Cobalto/química , Citomegalovirus/química , Sondas de ADN/química , Sondas de ADN/genética , Técnicas Electroquímicas , Humanos , Hibridación de Ácido Nucleico , Oxidación-Reducción , Proteínas del Envoltorio ViralRESUMEN
A major component of green tea, a widely consumed beverage, is (-)-epigallocatechin gallate (EGCG), which has strong antioxidant properties. Our previous study has indicated that free radical production following rat traumatic brain injury (TBI) induces neural degeneration. In this study, we investigated the effects of EGCG on cerebral function and morphology following TBI. Six-week-old male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG ad libitum, received TBI with a pneumatic controlled injury device at 10 weeks of age. Immunohistochemistry and lipid peroxidation studies revealed that at 1, 3 and 7 days post-TBI, the number of 8-hydroxy-2'-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells, and the levels of malondialdehyde (MDA) around the damaged area after TBI, significantly decreased in the EGCG treatment group compared with the water group (P < 0.05). Most ssDNA-positive cells in the water group co-localized with neuronal cells. However, in the EGCG treatment group, few ssDNA-positive cells co-localized with neurons. In addition, there was a significant increase in the number of surviving neuronal cells and an improvement in cerebral dysfunction after TBI in the EGCG treatment group compared with the water group (P < 0.05). These results indicate that consumption of water containing EGCG pre- and post-TBI inhibits free radical-induced neuronal degeneration and apoptotic cell death around the damaged area, resulting in the improvement of cerebral function following TBI. In summary, consumption of green tea may be an effective therapy for TBI patients.
Asunto(s)
Apoptosis/efectos de los fármacos , Lesiones Encefálicas/tratamiento farmacológico , Catequina/análogos & derivados , Corteza Cerebral/efectos de los fármacos , Trastornos del Conocimiento/tratamiento farmacológico , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/uso terapéutico , Animales , Catequina/uso terapéutico , Corteza Cerebral/lesiones , ADN de Cadena Simple/análisis , Peroxidación de Lípido/efectos de los fármacos , Masculino , Malondialdehído/análisis , Ratas , Ratas Wistar , Té/químicaRESUMEN
The backside contact, silicon-based transducers with vacuum-deposited gold layer (BSC) are evaluated as the base for electrochemical biosensors construction. Their comparison with commercially available transducers with screen printed gold and traditional gold disc electrode is reported. To determine the advantages and disadvantages of each of gold surfaces mentioned above, the 6-(ferrocenyl)-hexanethiol was used as the indicator. The results revealed the usefulness of BSC chips for the formation of stable self-assembled monolayers (SAMs). After the preliminary analysis, the SAM of thiol-ssDNA was formed on the BSC transducers as recognition layer. The electrochemical analysis in methylene blue solution was carried out after ssDNA immobilization and DNA-DNA hybridization. It is shown that prepared sensors are able to recognize complementary DNA sequence, based on the change in height and the potential shifts of reduction peaks of methylene blue. Obtained results are in full agreement with literature data. The compact size of silicone-based transducers allows to significantly reduce the required volume of tested solutions.
Asunto(s)
Técnicas Biosensibles/instrumentación , ADN/análisis , Oro/química , Silicio/química , Transductores , Técnicas Biosensibles/métodos , ADN/química , ADN/genética , ADN de Cadena Simple/análisis , ADN de Cadena Simple/química , ADN de Cadena Simple/genética , Electroquímica , Electrodos , Azul de Metileno/química , Hibridación de Ácido Nucleico , Análisis de Secuencia de ADN , Soluciones/química , Compuestos de Sulfhidrilo/químicaRESUMEN
A novel and sensitive electrochemical DNA biosensor has been developed for the detection of DNA hybridization. The biosensor was proposed by using copper(II) complex of Luteolin C(30)H(18)CuO(12) (CuL(2)) as an electroactive indicator based on silver nanoparticles and multi-walled carbon nanotubes (Ag/MWCNTs) modified glassy carbon electrode (GCE). In this method, the 4-aminobenzoic acid (4-ABA) and Ag nanoparticles were covalently grafted on MWCNTs to form Ag/4-ABA/MWCNTs. The proposed method dramatically increased DNA attachment quantity and complementary ssDNA detection sensitivity for its large surface area and good charge-transport characteristics. DNA hybridization detection was performed using CuL(2) as an electroactive indicator. The CuL(2) was synthesized and characterized using elemental analysis (EA) and IR spectroscopy. Cyclic voltammetry (CV) and fluorescence spectroscopy were used to investigate the interaction between CuL(2) and ds-oligonucleotides (dsDNA). It was revealed that CuL(2) presented high electrochemical activity on GCE, and it could be intercalated into the double helices of dsDNA. The target ssDNA of the human hepatitis B virus (HBV) was quantified in a linear range from 3.23x10(-12) to 5.31x10(-9) M (r=0.9983). A detection limit of 6.46x10(-13) M (3sigma, n=11) was achieved.
Asunto(s)
Técnicas Biosensibles/métodos , Cobre/química , ADN de Cadena Simple/análisis , Luteolina/química , Nanopartículas del Metal/química , Nanotubos de Carbono/química , Plata/química , Ácido 4-Aminobenzoico/química , Electrodos , Colorantes Fluorescentes/química , Virus de la Hepatitis B/genética , Humanos , Hibridación de Ácido Nucleico , Espectrometría de FluorescenciaRESUMEN
A flat microdevice which incorporates a thin-film amorphous silicon (a-Si:H) photodetector with an upper layer of functionalized SiO2 is used to quantify the density of both immobilized and hybridized DNA oligonucleotides labeled with a fluorophore. The device is based on the photoconductivity of hydrogenated amorphous silicon in a coplanar electrode configuration. Excitation, with near UV/blue light, of a single-stranded DNA molecule tagged with the fluorophore 1-(3-(succinimidyloxycarbonyl)benzyl)-4-(5-(4-methoxyphenyl)oxazol-2-yl) pyridinium bromide (PyMPO), results in the emission of visible light. The emitted light is then converted into an electrical signal in the photodetector, thus allowing the optoelectronic detection of the DNA molecules. The detection limit of the present device is of the order of 1 x 10(12) molecules/cm2 and is limited by the efficiency of the filtering of the excitation light. A surface density of 33.5 +/- 4.0 pmol/cm2 was measured for DNA covalently immobilized to the functionalized SiO2 thin film and a surface density of 3.7 +/- 1.5 pmol/cm2 was measured for the complementary DNA hybridized to the bound DNA. The detection concept explored can enable on-chip electronic data acquisition, improving both the speed and the reliability of DNA microarrays.
Asunto(s)
Emparejamiento Base , Sondas de ADN/análisis , Análisis de Secuencia por Matrices de Oligonucleótidos/instrumentación , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Secuencia de Bases , Calibración , Sondas de ADN/química , Sondas de ADN/genética , ADN de Cadena Simple/análisis , ADN de Cadena Simple/química , ADN de Cadena Simple/genética , Electrónica , Colorantes Fluorescentes/análisis , Luz , Hibridación de Ácido Nucleico , Reproducibilidad de los Resultados , Semiconductores , Sensibilidad y Especificidad , SilicioRESUMEN
This paper describes fabrication of serial microchamber arrays within the channels of a microfluidic device. The chambers are defined using a combination of weirs and UV-cross-linked hydrogel plugs (poly(ethylene glycol) diacrylates). This approach permits the microchambers to be addressed by pump-driven pressure in one dimension and by electrophoresis in the other. The function of the device is demonstrated by detecting DNA targets. Single-strand DNA (ssDNA) probes labeled with biotin were immobilized onto microbeads coated with streptavidin. The DNA-functionalized microbeads were packed into each of three microchambers by injection through inlet wells. Three oligonucleotides were designed as probes and four as targets. Hybridization reactions were performed by moving the targets across the array of probe-containing microchambers by electrophoresis. The hybridization of fluorescein-labeled ssDNA targets to complementary probes was observed by fluorescence microscopy. These studies resulted in four key observations: (1) there was no detectable binding of targets to noncomplementary probes; (2) hybridization was 90% complete within 1 min; (3) once captured, the targets could be independently released and recovered from the microbeads by treatment with 0.1 N NaOH; (4) multiple analyses could be performed using a single bead set, but there was degradation in performance after each capture/release cycle.
Asunto(s)
ADN de Cadena Simple/análisis , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Hidrogeles , Microscopía Fluorescente/métodos , Hibridación de Ácido Nucleico/métodos , Oligodesoxirribonucleótidos/análisis , Polietilenglicoles , Polímeros , Rayos UltravioletaRESUMEN
An optical sensor system based on evanescent field excitation of fluorophore-labeled DNA-targets specifically binding to immobilized DNA probes has been developed, thus enabling for real-time analysis of hybridization events. Oligonucleotide probes are directly immobilized on the surface of the disposable sensor chip via biotin/neutravidin linkage and hybridize to complementary Cy5-labeled target DNA in the sample; this is recorded as an increase in the fluorescence signal. Under optimized conditions the hybridization rate was constant and directly proportional to the target concentration. When an 18mer oligonucleotide was used as a probe a linear calibration curve was obtained for a 56mer single-stranded DNA target derived from the neomycin phosphotransferase gene, a selection marker in a variety of genetically modified plants, with an estimated lower limit of detection of 0.21 nmol L(-1). No cross-hybridization to a 51mer actin DNA target was observed and even a single-nucleotide mismatch led to a negligible signal. A shutter in the readout device enabled separate detection of targets hybridizing to probes immobilized at the inlet and outlet sides, respectively, of the flow channel. This opens a route toward a real-time DNA array format with analysis times as short as 1-2 min. As a realistic sample a Cy5-labeled 56 bp PCR product was measured after separation of the double-stranded DNA by simple heat denaturation with a detection limit clearly lower than that of traditional gel electrophoresis.