RESUMEN
Human footprints at White Sands National Park, New Mexico, USA, reportedly date to between ~23,000 and 21,000 years ago according to radiocarbon dating of seeds from the aquatic plant Ruppia cirrhosa. These ages remain controversial because of potential old carbon reservoir effects that could compromise their accuracy. We present new calibrated 14C ages of terrestrial pollen collected from the same stratigraphic horizons as those of the Ruppia seeds, along with optically stimulated luminescence ages of sediments from within the human footprint-bearing sequence, to evaluate the veracity of the seed ages. The results show that the chronologic framework originally established for the White Sands footprints is robust and reaffirm that humans were present in North America during the Last Glacial Maximum.
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Evolución Biológica , Hominidae , Animales , Humanos , Luminiscencia , América del Norte , Datación Radiométrica/métodos , New Mexico , Parques Recreativos , Polen , Alismatales , Radioisótopos de Carbono , SemillasRESUMEN
Since the banning of tributyltin, the addition of inorganic (metal oxides) and organic (pesticides, herbicides) biocides in antifouling paint has represented an unavoidable step to counteract biofouling and the resulting biodeterioration of submerged surfaces. Therefore, the development of new methods that balance antifouling efficacy with environmental impact has become a topic of great importance. Among several proposed strategies, natural extracts may represent one of the most suitable alternatives to the widely used toxic biocides. Posidonia oceanica is one of the most representative organisms of the Mediterranean Sea and contains hundreds of bioactive compounds. In this study, we prepared, characterized, and assessed a hydroalcoholic extract of P. oceanica and then compared it to three model species. Together, these four species belong to relevant groups of biofoulers: bacteria (Aliivibrio fischeri), diatoms (Phaeodactylum tricornutum), and serpulid polychaetes (Ficopomatus enigmaticus). We also added the same P. oceanica extract to a PDMS-based coating formula. We tested this coating agent with Navicula salinicola and Ficopomatus enigmaticus to evaluate both its biocidal performance and its antifouling properties. Our results indicate that our P. oceanica extract provides suitable levels of protection against all the tested organisms and significantly reduces adhesion of N. salinicola cells and facilitates their release in low-intensity waterflows.
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Alismatales , Incrustaciones Biológicas , Diatomeas , Desinfectantes , Herbicidas , Desinfectantes/toxicidad , Incrustaciones Biológicas/prevención & control , Extractos VegetalesRESUMEN
Understanding species-specific trait responses under future global change scenarios is of importance for conservation efforts and to make informed decisions within management projects. The combined and single effects of seawater acidification and warmer average temperature were investigated by means of the trait responses of Cymodocea serrulata, a tropical seagrass, under experimental conditions. After a 35 d exposure period, biochemical, morphological, and photo-physiological trait responses were measured. Overall, biochemical traits mildly responded under the individual exposure to high temperature and increasing pCO2 values. The response of C. serrulata was limited to a decrease in %C and an increase in the sucrose content in the rhizome under the high temperature treatment, 32 °C. This suggests that this temperature was lower than the maximum tolerance limit for this species. Increasing pCO2 levels increased %C in the rhizome, and also showed a significant increase in leaf δ13C values. The effects of all treatments were sublethal; however, small changes in their traits could affect the ecosystem services they provide. In particular, changes in tissue carbon concentrations may affect carbon storage capacity, one key ecosystem service. The simultaneous study of different types of trait responses contributes to establish a holistic framework of seagrass ecosystem health under climate change.
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Alismatales , Agua de Mar , Agua de Mar/química , Ecosistema , Alismatales/fisiología , Calor , Temperatura , Carbono , Dióxido de Carbono , Concentración de Iones de HidrógenoRESUMEN
Posidonia oceanica (L.) Delile is an endemic Mediterranean marine plant of extreme ecological importance. Previous in vitro and in vivo studies have demonstrated the potential antidiabetic properties of P. oceanica leaf extract. Intestinal glucose transporters play a key role in glucose homeostasis and represent novel targets for the management of diabetes. In this study, the ability of a hydroalcoholic P. oceanica leaf extract (POE) to modulate intestinal glucose transporters was investigated using Caco-2 cells as a model of an intestinal barrier. The incubation of cells with POE significantly decreased glucose uptake by decreasing the GLUT2 glucose transporter levels. Moreover, POE had a positive effect on the barrier integrity by increasing the Zonulin-1 levels. A protective effect exerted by POE against oxidative stress induced by chronic exposure to high glucose concentrations or tert-butyl hydroperoxide was also demonstrated. This study highlights for the first time the effect of POE on glucose transport, intestinal barrier integrity, and its protective antioxidant effect in Caco-2 cells. These findings suggest that the P. oceanica phytocomplex may have a positive impact by preventing the intestinal cell dysfunction involved in the development of inflammation-related disease associated with oxidative stress.
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Alismatales , Células CACO-2 , Glucosa , Humanos , Estrés Oxidativo , Extractos Vegetales/farmacologíaRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Zexie Tang (ZXT), only two consists with Alismatis Rhizoma (AR) and Atractylodes macrocephala Rhizoma (AM), a classical Chinese medicine formula from Synopsis of the Golden Chamber with a history of 2000 years. Clinical observation in recent years has found that ZXT has excellent lipid-lowering effect. AIM OF THE STUDY: To explore the potential mechanism of ZXT ameliorates hyperlipidemia based on FKBP38/mTOR/SREBPs pathway. MATERIALS AND METHODS: WD-induced hyperlipidemia mice and oleic acid induced cell lipid accumulation model were used to investigate pharmacodynamic. The effect of ZXT on the transcriptional activity of SREBPs was detected by reporter gene assay. Proteins and downstream genes of mTOR/SREBPs pathway were detected in vivo and in vitro. Combined with network pharmacology and HPLC-Q-TOF/MS, the active ingredients were screened and identified. The interaction between active compounds of ZXT and FKBP38 protein were analyzed by docking analysis. RESULTS: ZXT decreased TC, TG and LDL-c levels in blood of WD-induced hyperlipidemia mouse model, and improved insulin resistance in vivo. ZXT also reduced TC, TG and lipid accumulation in cells line, and inhibited SREBPs luciferase activity, protein and its target genes expression such as FASN, HMGCR, etc. Meanwhile, ZXT inhibited protein expression levels of p-mTOR, p-S6K, etc in vitro and in vivo. Combined with network pharmacology and HPLC-Q-TOF/MS, 16 active ingredients were screened and identified. Docking results showed that active compounds of ZXT binding to FKBP38 and formed hydrogen bond. CONCLUSION: Our findings highlighted that ZXT ameliorates hyperlipidemia, in which FKBP/mTOR/SREBPs pathway might be the potential regulatory mechanism.
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Hiperlipidemias/patología , Lípidos/sangre , Extractos Vegetales/farmacología , Proteínas de Unión a los Elementos Reguladores de Esteroles/efectos de los fármacos , Serina-Treonina Quinasas TOR/efectos de los fármacos , Proteínas de Unión a Tacrolimus/efectos de los fármacos , Alismatales , Animales , Atractylodes , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Masculino , Ratones , Ratones Endogámicos C57BL , Simulación del Acoplamiento Molecular , Farmacología en RedRESUMEN
The marine environment is a generous source of biologically active compounds useful for human health. In 50 years, about 25,000 bioactive marine compounds have been identified, with an increase of 5% per year. Peculiar feature of algae and plants is the production of secondary metabolites, such as polyphenols, synthesized as a form of adaptation to environmental stress. Posidonia oceanica is a Mediterranean endemic and dominant seagrass and represents a biologically, ecologically and geologically important marine ecosystem. Within this study, methanolic and ethanolic extracts were generated from fresh and dried Posidonia oceanica leaves, with the aim to employ and valorize the beach cast leaves. The best yield and antioxidant activity (polyphenols content equal to 19.712 ± 0.496 mg GAE/g and DPPH IC50 of 0.090 µg/µL.) were recorded in 70% ethanol extracts (Gd-E4) obtained from leaves dried for two days at 60 °C and ground four times. HPLC analyses revealed the presence of polyphenols compounds (the most abundant of which was chicoric acid) with antioxidant and beneficial properties. Bioactive properties of the Gd-E4 extracts were evaluated in vitro using fibroblast cells line (HS-68), subjected to UV induced oxidative stress. Pre-treatment of cells with Gd-E4 extracts led to significant protection against oxidative stress and mortality associated with UV exposure, thus highlighting the beneficial properties of antioxidants compounds produced by these marine plants against photo damage, free radicals and associated negative cellular effects. Beach cast leaves selection, processing and extraction procedures, and the in vitro assay results suggested the potentiality of a sustainable approach for the biotechnological exploitation of this resource and could serve a model for other marine resources.
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Alismatales , Antioxidantes/farmacología , Extractos Vegetales/farmacología , Antioxidantes/química , Organismos Acuáticos , Compuestos de Bifenilo , Ecosistema , Fibroblastos/efectos de los fármacos , Humanos , Mar Mediterráneo , Estrés Oxidativo/efectos de los fármacos , Picratos , Extractos Vegetales/química , Hojas de la PlantaRESUMEN
Neuroblastoma (NB) is a common cancer in childhood, and lethal in its high-risk form, primarily because of its high metastatic potential. Targeting cancer cell migration, and thus preventing metastasis formation, is the rationale for more effective cancer therapy against NB. Previous studies have described the leaf extract from Posidonia oceanica marine plant (POE) as an antioxidant, anti-inflammatory agent and inhibitor of cancer cell migration. This study aims to examine the POE anti-migratory role in human SH-SY5Y neuroblastoma cells and the underlying mechanisms of action. Wound healing and gelatin zymography assays showed that POE at early times inhibits cell migration and reduces pro-MMP-2 release into culture medium. By monitoring expression level of key autophagy markers by Western blot assay, a correlation between POE-induced cell migration inhibition and autophagy activation was demonstrated. Cell morphology and immunofluorescence analyses showed that POE induces neurite formation and neuronal differentiation at later times. These results suggest POE might act against cell migration by triggering early nontoxic autophagy. The POE-induced cellular morphological change toward cell differentiation might contribute to prolonging the phytocomplex anti-migratory effect to later times. Overall, these results encourage future in vivo studies to test POE applicability in neuroblastoma treatment.
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Alismatales , Antineoplásicos/farmacología , Línea Celular Tumoral/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Antineoplásicos/química , Antineoplásicos/uso terapéutico , Organismos Acuáticos , Movimiento Celular/efectos de los fármacos , Humanos , Neuroblastoma/diagnóstico por imagen , Fitoterapia , Extractos Vegetales/química , Extractos Vegetales/uso terapéuticoRESUMEN
Posidonia oceanica (L.) Delile is a Mediterranean-endemic angiosperm often described for its great ecological importance. Despite evidence of a millennia-old relationship between P. oceanica and humans, as well as traditional medicine applications, the potential benefits of P. oceanica for human health have been documented only recently. This review aims to compile newly acquired knowledge on P. oceanica bioactive properties that allow the scientific community to look at this plant as a promising source of natural therapeutical products for human health. Experimental investigations conducted in both in vitro cellular-based and in vivo animal models pave the way for new research projects aiming at the development of alternative and complementary therapeutic strategies based on P. oceanica against a wide range of pathological conditions.
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Alismatales , Productos Biológicos/uso terapéutico , Animales , Ecosistema , Humanos , Medicina Tradicional , Mar Mediterráneo , Hojas de la PlantaRESUMEN
A lignin fraction (LF) was extracted from the sea balls of Posidonia oceanica (egagropili) and extensively dialyzed and characterized by FT-IR and NMR analyses. LF resulted water soluble and exhibited a brownish-to-black color with the highest absorbance in the range of 250-400 nm, attributed to the chromophore functional groups present in the phenylpropane-based polymer. LF high-performance size exclusion chromatography analysis showed a highly represented (98.77%) species of 34.75 kDa molecular weight with a polydispersity index of 1.10 and an intrinsic viscosity of 0.15. Quantitative analysis of carbohydrates indicated that they represented 28.3% of the dry weight of the untreated egagropili fibers and 72.5% of that of LF. In particular, eight different monosaccharides were detected (fucose, arabinose, rhamnose, galactose, glucose, xylose, glucosamine and glucuronic acid), glucuronic acid (46.6%) and rhamnose (29.6%) being the most present monosaccharides in the LF. Almost all the phenol content of LF (113.85 ± 5.87 mg gallic acid eq/g of extract) was water soluble, whereas around 22% of it consisted of flavonoids and only 10% of the flavonoids consisted of anthocyanins. Therefore, LF isolated from egagropili lignocellulosic material could be defined as a water-soluble lignin/carbohydrate complex (LCC) formed by a phenol polymeric chain covalently bound to hemicellulose fragments. LCC exhibited a remarkable antioxidant activity that remained quite stable during 6 months and could be easily incorporated into a protein-based film and released from the latter overtime. These findings suggest egagropili LCC as a suitable candidate as an antioxidant additive for the reinforcement of packaging of foods with high susceptibility to be deteriorated in aerobic conditions.
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Alismatales/química , Antioxidantes/química , Lignina/química , Monosacáridos/química , Extractos Vegetales/química , Ácido Gálico/química , Glucosamina/química , Fenoles/química , Proteínas/química , ViscosidadRESUMEN
Posidonia oceanica (L.) Delile is traditionally used for its beneficial properties. Recently, promising antioxidant and anti-inflammatory biological properties emerged through studying the in vitro activity of the ethanolic leaves extract (POE). The present study aims to investigate the anti-inflammatory and analgesic role of POE in mice. Inflammatory pain was modeled in CD-1 mice by the intraplantar injection of carrageenan, interleukin IL-1ß and formalin. Pain threshold was measured by von Frey and paw pressure tests. Nociceptive pain was studied by the hot-plate test. POE (10-100 mg kg-1) was administered per os. The paw soft tissue of carrageenan-treated animals was analyzed to measure anti-inflammatory and antioxidant effects. POE exerted a dose-dependent, acute anti-inflammatory effect able to counteract carrageenan-induced pain and paw oedema. Similar anti-hyperalgesic and anti-allodynic results were obtained when inflammation was induced by IL-1ß. In the formalin test, the pre-treatment with POE significantly reduced the nocifensive behavior. Moreover, POE was able to evoke an analgesic effect in naïve animals. Ex vivo, POE reduced the myeloperoxidase activity as well as TNF-α and IL-1ß levels; further antioxidant properties were highlighted as a reduction in NO concentration. POE is the candidate for a new valid strategy against inflammation and pain.
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Alismatales , Analgésicos/uso terapéutico , Antiinflamatorios/uso terapéutico , Mediadores de Inflamación/antagonistas & inhibidores , Dolor/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Analgésicos/aislamiento & purificación , Analgésicos/farmacología , Animales , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Mediadores de Inflamación/metabolismo , Ratones , Dolor/metabolismo , Dimensión del Dolor/efectos de los fármacos , Dimensión del Dolor/métodos , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Hojas de la Planta , Resultado del TratamientoRESUMEN
OBJECTIVES: The study aimed to explore the anti-inflammatory effect, underlying mechanism, and chemistry of Halodule pinifolia extract. METHODS: The ethyl acetate (EHP) and methanol (MHP) extracts of Halodule pinifolia were screened for pro-inflammatory cytokine inhibition effect under various in vitro (LPSand crystal-induced inflammation) and in vivo models (LPS-induced endotoxaemia model, carrageenan-induced paw oedema model, and oxalate-induced renal nephropathy model of inflammation). The effect of EHP on the expression of inflammatory markers using western blot analysis (in vitro) was investigated. Chemical constituents of bioactive EHP were isolated through chromatography and characterised using NMR spectroscopy. Furthermore, EHP was standardised for rosmarinic acid, vanillic acid, and ethyl protocatechuate using HPLC. Also, total phytosterols, phenolic, and flavonoid content of EHP were determined by UV spectroscopy. KEY FINDINGS: EHP was comparatively more effective than MHP in inhibiting cytokines secretions under LPS-induced in vitro models. Furthermore, EHP was screened under endotoxaemia in vivo model, EHP (250 mg/kg) reduced plasma IL-6, TNF-α, and IL-1ß levels by 88.3%, 78.2%, and 74.5%, respectively. In the carrageenan-induced oedema model, EHP (200 mg/kg) reduced paw volume and release of TNF-α (69.3%) and IL-1ß (43.1%). EHP (200 mg/kg) further controlled renal nephropathy by inhibiting plasma IL-1ß and BUN levels. Also, a significant reduction of mRNA expressions of TNF-α and IL-1ß and KIM-1 in renal tissues was observed. Through western blot, EHP was identified to regulate the expression of pro-form as well as mature-form of IL-1ß and caspase-1. EHP constituted rosmarinic acid (RA), vanillic acid (VA), ethyl protocatechuate (EP), sitosterol, stigmasterol, campesterol, and dihydrobrassicasterol. It was determined that 4.6 mg/g of RA, 2.92 mg/g of VA, 0.76 mg/g of EP, 21.7 mg/g of total phenolics, 29.8 mg/g of total flavonoids, and 48.2 mg/g of total phytosterols were present in dry EHP. The presence of anti-inflammatory constituents such as RA, VA, and PE in EHP corroborated the in vitro and in vivo anti-inflammatory activity of EHP. CONCLUSION: The anti-inflammatory property of EHP and its action through attenuation of pan-cytokines suggest that it can be developed into an oral pharmaceutical drug.
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Alismatales/química , Antiinflamatorios/farmacología , Inflamación/tratamiento farmacológico , Extractos Vegetales/farmacología , Acetatos/química , Animales , Antiinflamatorios/administración & dosificación , Antiinflamatorios/aislamiento & purificación , Carragenina , Citocinas/metabolismo , Modelos Animales de Enfermedad , Edema/tratamiento farmacológico , Inflamación/patología , Lipopolisacáridos , Masculino , Metanol/química , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Extractos Vegetales/administración & dosificaciónRESUMEN
Recent developments in breast cancer therapy have significantly improved patient survival rate; however, recurrence remains a major problem. Systemic treatment of breast cancer with available therapies is not curative. Natural products can be potentially used for treating cancer. Recently, a wide range of pharmacological activities has been reported for Alismatis Rhizoma, a popular traditional Chinese medicine. However, the mechanisms via which its compounds act on breast cancer remain unclear. The present study aimed to investigate the potential of natural therapeutic agents from Alismatis Rhizoma for treating breast cancer. Human breast cancer MDAMB231 cells were treated with four main protostane triterpenes from Alismatis Rhizoma, including alisol A, alisol A 24acetate, alisol B and alisol B 23acetate. Among these, alisol A significantly inhibited cell viability. Alisol A induced cell apoptosis, G1 phase cell cycle arrest, autophagy, and intracellular reactive oxygen species (ROS) generation in MDAMB231 cells. The number of APE1/γH2AX/LC3II positive cells was also significantly higher compared with that of negative control cells. All these results were dosedependent. Cleaved caspase3, cleaved caspase 9, Bcl2, and pp38 expression indicated cell apoptosis after alisol A treatment. The changes in cyclin A and cyclin D1 expression was associated with cell cycle arrest upon alisol A treatment. Furthermore, LC3II expression upon alisol A treatment was indicative of autophagy. Alisol A treatment can induce autophagydependent apoptosis in human breast cancer cells via induction of ROS and DNA damage. Thus, Alisol A might serve as a new therapeutic agent against breast cancer.
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Neoplasias de la Mama/tratamiento farmacológico , Colestenonas/farmacología , Alismatales/química , Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Autofagia/efectos de los fármacos , Neoplasias de la Mama/genética , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Daño del ADN , Femenino , Puntos de Control de la Fase G1 del Ciclo Celular/efectos de los fármacos , Humanos , Especies Reactivas de Oxígeno/metabolismo , Rizoma/químicaRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: The marine plant Posidonia oceanica (L.) Delile is traditionally used by villagers of the west coast of Anatolia as a remedy for diabetes and hypertension. AIM OF THE STUDY: The aim of this study was to explore the role of the P. oceanica hydroalcoholic leaves extract (POE) against human serum albumin glycation. MATERIAL AND METHODS: Advanced glycation end products (AGEs) were obtained with the albumin-glucose in vitro assay. The AGEs intrinsic fluorescence intensity and the electrophoretic migration under native conditions allowed us to verify the effective glycation of albumin. The presence of POE during glycation process was intended to evaluate its anti-glycation role. RESULTS: POE exhibited a strong in vitro anti-glycation ability which occurred independently from its known antioxidant property. CONCLUSIONS: Overall, the antidiabetic, antioxidant, anti-inflammatory and anti-glycation properties of POE could be exploited as an effective tool against diabetes and related complications.
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Alismatales , Productos Finales de Glicación Avanzada/antagonistas & inhibidores , Hipoglucemiantes/farmacología , Extractos Vegetales/farmacología , Albúmina Sérica Humana/antagonistas & inhibidores , Alismatales/química , Antiinflamatorios/farmacología , Antioxidantes/farmacología , Hipoglucemiantes/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Hojas de la PlantaRESUMEN
PREMISE: Water-pollination (hydrophily) is a rare but important pollination mechanism that has allowed angiosperms to colonize marine and aquatic habitats. Hydrophilous plants face unique reproductive challenges, and many have evolved characteristic pollen traits and pollination strategies that may have downstream consequences for pollen performance. However, little is known about reproductive development in the life history stage between pollination and fertilization (the progamic phase) in hydrophilous plants. The purpose of this study was to characterize reproductive ecology and postpollination development in water-pollinated Ruppia maritima L. METHODS: Naturally pollinated inflorescences of R. maritima were collected from the field. Experimental pollinations using both putatively outcross and self pollen were conducted in the greenhouse and inflorescences were collected at appropriate intervals after pollination. Pollen reception, pollen germination, pollen tube growth, and carpel morphology were characterized. RESULTS: Ruppia maritima exhibits incomplete protogyny, allowing for delayed selfing. Pollen germinated within 15 min after pollination. The average shortest possible pollen tube pathway was 425 µm and pollen tubes first reached the ovule at 45 min after pollination. The mean adjusted pollen tube growth rate was 551 µm/h. CONCLUSIONS: Ruppia pollen is adapted for rapid pollen germination, which is likely advantageous in an aquatic habitat. Small effective pollen loads suggest that pollen competition intensity is low. Selection for traits such as a long period of stigma receptivity, fast pollen germination, and carpel morphology likely played a larger role in shaping postpollination reproductive development in Ruppia than evolution in pollen tube growth rates.
Asunto(s)
Alismatales , Magnoliopsida , Polen , Polinización , ReproducciónRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Posidonia oceanica (L.) Delile is an endemic seagrass of the Mediterranean Sea whose use has been documented as a traditional herbal remedy for diabetes and hypertension. Our recently described Posidonia oceanica leaves extract is a phytocomplex endowed with interesting bioactivities, including the inibitory property on human cancer cell migration. AIM OF THE STUDY: The aim of this study was to investigate the anti-inflammatory effects of P. oceanica extract underlying its mechanism of action. MATERIALS AND METHODS: We explored the anti-inflammatory effects of P. oceanica extract on RAW264.7 murine macrophages activated by LPS. We investigated the reactive oxygen species (ROS) and nitric oxide (NO) production and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Then, we examined P. oceanica extract role on the regulation of NF-κB signaling pathway. RESULTS: P. oceanica phytocomplex exhibited a strong ability to inhibit oxidative stress by affecting the production of both ROS and NO and to reduce iNOS and COX-2 levels. In addition, it was evidenced its anti-inflammatory role via inhibiting NF-κB signaling pathway through modulation of ERK1/2 and Akt intracellular cascades. CONCLUSIONS: Our results recognize an anti-inflammatory role of P. oceanica phytocomplex particularly emphasizing its cell safe mechanism of action. In conclusion, the marine plant P. oceanica may be of great interest for scientific research as a source of promising molecules for designing alternative strategies to the conventional treatment of inflammatory diseases.
Asunto(s)
Alismatales/química , Antiinflamatorios/farmacología , Organismos Acuáticos/química , Extractos Vegetales/farmacología , Animales , Antiinflamatorios/aislamiento & purificación , Ciclooxigenasa 2/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/inmunología , Mar Mediterráneo , Ratones , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/inmunología , Extractos Vegetales/aislamiento & purificación , Células RAW 264.7 , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Historically, extensive seagrass meadows were common throughout the Indian River Lagoon (IRL) in east-central Florida, USA. Between 2011 and 2017, widespread catastrophic seagrass losses (~95%) occurred in the IRL following unprecedented harmful algal blooms (HABs), including persistent brown tides (Aureoumbra lagunensis). Little is known about how dissolved nutrients and chlorophyll a are related to light limitation or how biochemical factors, such as the elemental composition (C:N:P) and stable isotope signatures (δ13C, δ15N), of seagrasses within the IRL relate to coverage. Accordingly, we conducted a survey from 2013 to 2015 at 20 sites to better understand these relationships. Results showed a negative correlation between DIN and salinity, indicating freshwater inputs as a DIN source. Seawater N:P ratios and chlorophyll a concentrations were higher in the urbanized, poorly-flushed northern IRL segments. Kd values were higher in the wet season and often exceeded seagrass light requirements (0.8â¯m-1) for restoration, demonstrating light limitation. Species distribution varied by location. Halodule wrightii was ubiquitous, whereas Syringodium filiforme was not found in the northernmost segments. Thalassia testudinum was only present in the two southernmost segments that had the lowest TDN and highest light availability (Kd). Blade %N and %P also frequently exceeded critical values of 1.8% and 0.2%, respectively, especially in the northern segments. Further, δ15N was positively correlated with ammonium, suggesting wastewater as a major N source. The δ13C values indicated a trend of increasing light limitation from south to north, which helps explain the recent catastrophic loss of seagrasses in the northern IRL. Overall, elemental composition reflected high N-availability and seagrass species distributions were relatable to spatial trends in N and light limitation. For effective restoration, resource managers must reduce N-loading to the IRL to diminish HABs and increase light availability. Regular biochemical monitoring of seagrass tissue should also be implemented during restoration efforts.
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Ecosistema , Estuarios , Ríos , Contaminantes Químicos del Agua/análisis , Alismatales , Clorofila A , Monitoreo del Ambiente , Florida , Nitrógeno/análisis , Fósforo/análisis , Agua de Mar , UrbanizaciónRESUMEN
The marine environment is constantly at risk of pollution by hydrocarbon spills that requires its cleanup to protect the environment and human health. Posidonia oceanica (L.) (PO) beach balls, which are characteristic of the Mediterranean Sea and abundant on the beaches, are used as biosorbent to remove hydrocarbons from the sea. The impact of several factors such as oil concentration, time sorption, and weight sorbent was investigated to determine the oil and water sorption capacity for raw and milled P. oceanica fibers. The study of kinetic models for initial crude oil concentration of 2.5, 5, 8.8, 10, 15, 20, 30, and 40 g/L revealed that crude uptake followed the pseudo-first-order model while, for isotherm models, the crude uptake onto the P. oceanica tended to fit the Langmuir model. Experiments were performed according to two systems: a pure oil and pure water system and a mixed oil/water system. For the dry system (pure oil and pure water), the maximum oil and water sorption capacity of raw and milled fibers was found to be 5.5 g/g and 14 g/g for oil and 14.95 g/g and 15.84 g/g for water, respectively, whereas, in the mixed oil/water system, the maximum oil and water sorption capacity was estimated as 4.74 g/g, 12.80 g/g and 7.41 g/g, 8.31 g/g, respectively. The results showed that, in spite of their absorbency of a lot of water, the milled fibers with grain size ranging between 0.5 mm and 1 mm might be the relevant sorbent for the elimination of crude oil from seawater thanks to its efficient sorption capacity and low cost.
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Alismatales/metabolismo , Biodegradación Ambiental , Contaminación por Petróleo , Petróleo , Agua de Mar/química , Cinética , Mar Mediterráneo , Tamaño de la Partícula , Petróleo/análisis , Petróleo/metabolismoRESUMEN
The present study is to establish a quantitative analysis of multi-components by single marker(QAMS) for determining contents of seven compositions in Alismatis Rhizoma, alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B. Six relative correction factors(RCFs) of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B and 11-deoxy-alisol B were established in the UPLC method with alisol B 23-acetate as the internal standard, which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in Alismatis Rhizoma was calculated by the external standard method(ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Within the linear range, the RCFs of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, 11-deoxy-alisol B were 0.946, 4.183, 0.915, 1.039, 0.923 and 1.244, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. Then, QAMS method was applied to determination of the different degree Alismatis Rhizoma from different areas. As a result, the concentrations of 7 components have differences in different areas, but no significant differences in different grades. The QAMS method is feasible and accurate for the determination of the seven chemical compositions, and which can be used for quality control of Alismatis Rhizoma.
Asunto(s)
Alismatales/química , Medicamentos Herbarios Chinos/análisis , Fitoquímicos/análisis , Rizoma/químicaRESUMEN
Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated. Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble). Moreover, a conventional protocol with organic solvents was applied, yielding E3 extracts. Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates. All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides. All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus. These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
Asunto(s)
Alismatales/química , Antiinfecciosos/farmacología , Antioxidantes/farmacología , Fitoquímicos/farmacología , Extractos Vegetales/farmacocinética , Animales , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Biomasa , Infecciones por Caliciviridae/tratamiento farmacológico , Infecciones por Caliciviridae/virología , Calicivirus Felino/efectos de los fármacos , Gatos , Etanol/química , Enfermedades Transmitidas por los Alimentos/microbiología , Enfermedades Transmitidas por los Alimentos/prevención & control , Humanos , Lípidos/química , Lípidos/aislamiento & purificación , Lípidos/farmacología , Ratones , Pruebas de Sensibilidad Microbiana , Hongos Mitospóricos/efectos de los fármacos , Norovirus/efectos de los fármacos , Fenoles/química , Fenoles/aislamiento & purificación , Fenoles/farmacología , Fitoquímicos/química , Fitoquímicos/aislamiento & purificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Células RAW 264.7 , Solventes/química , Agua/químicaRESUMEN
BACKGROUND: The present investigation looks at the most likely possibilities of usage of a naturally occurring photosynthetic pigment, Pheophytin a, from the seagrass, Syringodium isoetifolium, for plausible use as human TSPO ligand. METHODS: Pheophytin a isolated in our laboratory previously was administered to A549 cell lines in vitro to examine its effects on cell migrations, DNA, cell cycle, Mitochondrial Membrane Potential and gene expressions. In silico tools were used to predict the nature of the compound and target binding. RESULTS: Pheophytin a hadIC50 values of 22.9⯱â¯5.8⯵M for cancerous A549 cell lines, whilst not targeting non-cancerous vero cells [IC50: 183.6⯱â¯1.92⯵M]. Pheophytin a hindered cellular migration, fragmented DNA, arrested cell cycle precisely at S phase, reduced ∆ψmit and directed mRNA expressions toward apoptosis. In silico tools indicate that the compound binds to TSPO with high effectiveness to collapse ∆ψmit(which is proved using wet lab experiments) to promote mitophagy. CONCLUSION: Hence Pheophytin a could be seen as a possible TSPO ligand for targeting metastatic alveolar cancers like A549 via intrinsic apoptotic pathway. GENERAL SIGNIFICANCE: Given the inherent non-toxic nature of the compound and easy extractability from almost all autotrophic eukaryotes, one could be confident to testing in animal models.