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Métodos Terapéuticos y Terapias MTCI
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1.
Int J Biol Macromol ; 108: 916-926, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29113895

RESUMEN

Hydrocolloids from seaweeds (phycocolloids) have interesting functional properties like antiproliferative activity. Marine algae consumptions are linked to law cancer incidences in countries that traditionally consume marine products. In this study, we have investigated water-soluble sulfated polysaccharides isolated from the red seaweed Laurencia papillosa and determined their chemical characteristics and biological activities on the human breast cancer cell line MCF-7. Total polysaccharides were extracted and fractionated from L. papillosa and characterized using FTIR-ATR and NMR spectrometry. In addition, their approximate molar mass was determined by GPC method. The chemical characterization of purified polysaccharides reveals the presence of sulfated polysaccharides differentially dispersed in the algal cell wall. They are the three types of carrageenan, kappa, iota and lambda carrageenans, named LP-W1, -W2 and -W3 respectively. Biological effects and cytotoxicity of the identified of the three sulfated polysaccharide fractions were evaluated in MCF-7 cell line. Our results showed a significant inhibition of MCF-7 cell viability by dose-dependent manner for cells exposed to LP-W2 and LP-W3 polysaccharides for 24h. The mechanistic of LP fractions-mediated apoptosis in MCF-7 cells was demonstrated. The biological effects of L. papillosa SPs indicate that it may be a promising candidate for breast cancer prevention and therapy.


Asunto(s)
Antineoplásicos/química , Antineoplásicos/farmacología , Coloides/química , Coloides/farmacología , Laurencia/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Antineoplásicos/aislamiento & purificación , Apoptosis/efectos de los fármacos , Biomarcadores , Neoplasias de la Mama , Proliferación Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Coloides/aislamiento & purificación , Citometría de Flujo , Humanos , Células MCF-7 , Espectroscopía de Resonancia Magnética , Peso Molecular , Extractos Vegetales/aislamiento & purificación , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Espectroscopía Infrarroja por Transformada de Fourier , Sulfatos/química
2.
Food Res Int ; 99(Pt 3): 1066-1083, 2017 09.
Artículo en Inglés | MEDLINE | ID: mdl-28865618

RESUMEN

Edible seaweeds are a good source of antioxidants, dietary fibers, essential amino acids, vitamins, phytochemicals, polyunsaturated fatty acids, and minerals. Many studies have evaluated the gelling, thickening and therapeutic properties of seaweeds when they are used individually. This review gives an overview on the nutritional, textural, sensorial, and health-related properties of food products enriched with seaweeds and seaweed extracts. The effect of seaweed incorporation on properties of meat, fish, bakery, and other food products were highlighted in depth. Moreover, the positive effects of foods enriched with seaweeds and seaweed extracts on different lifestyle diseases such as obesity, dyslipidemia, hypertension, and diabetes were also discussed. The results of the studies demonstrated that the addition of seaweeds, in powder or extract form, can improve the nutritional and textural properties of food products. Additionally, low-fat products with less calories and less saturated fatty acids can be prepared using seaweeds. Moreover, the addition of seaweeds also affected the health properties of food products. The results of these studies demonstrated that the health value, shelf-life and overall quality of foods can be improved through the addition of either seaweeds or seaweed extracts.


Asunto(s)
Dieta Saludable , Suplementos Dietéticos , Conservación de Alimentos/métodos , Conservantes de Alimentos/farmacología , Almacenamiento de Alimentos/métodos , Preparaciones de Plantas/farmacología , Algas Marinas/química , Animales , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Restricción Calórica , Coloides/aislamiento & purificación , Coloides/farmacología , Dieta con Restricción de Grasas , Conservantes de Alimentos/aislamiento & purificación , Humanos , Valor Nutritivo , Preparaciones de Plantas/aislamiento & purificación , Factores de Tiempo
3.
Int J Mol Sci ; 16(8): 19291-307, 2015 Aug 14.
Artículo en Inglés | MEDLINE | ID: mdl-26287178

RESUMEN

Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEON(LA-BSA), which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEON(LA-BSA) particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEON(LA-BSA) changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic hyperthermia is greatly enhanced from 44.4 °C up to 64.9 °C by the concentration of the particles up to 16.9 mg/mL total iron. Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.


Asunto(s)
Ácidos Láuricos/química , Nanopartículas de Magnetita/química , Albúmina Sérica Bovina/química , Ultrafiltración/métodos , Animales , Bovinos , Coloides/química , Coloides/aislamiento & purificación , Humanos , Hipertermia Inducida , Células Jurkat , Ácidos Láuricos/aislamiento & purificación , Magnetismo , Albúmina Sérica Bovina/aislamiento & purificación , Propiedades de Superficie
4.
Int J Biol Macromol ; 61: 142-9, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23817104

RESUMEN

Microwave-assisted extraction (MAE) technique was employed to extract the hydrocolloid from okra pods (OPH). The optimal conditions for microwave-assisted extraction of OPH were determined by response surface methodology. A central composite rotatable design (CCRD) was applied to evaluate the effects of three independent variables (microwave power (X1: 100-500 W), extraction time (X2: 30-90 min), and extraction temperature (X3: 40-90 °C)) on the extraction yield of OPH. The correlation analysis of the mathematical-regression model indicated that quadratic polynomial model could be employed to optimize the microwave extraction of OPH. The optimal conditions to obtain the highest recovery of OPH (14.911±0.27%) were as follows: microwave power, 395.56 W; extraction time, 67.11 min and extraction temperature, 73.33 °C. Under these optimal conditions, the experimental values agreed with the predicted ones by analysis of variance. It indicated high fitness of the model used and the success of response surface methodology for optimizing OPH extraction. After method development, the DPPH radical scavenging activity of the OPH was evaluated. MAE showed obvious advantages in terms of high extraction efficiency and radical scavenging activity of extract within the shorter extraction time.


Asunto(s)
Abelmoschus/química , Fraccionamiento Químico/métodos , Coloides/química , Coloides/aislamiento & purificación , Frutas/química , Microondas , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Coloides/farmacología , Modelos Teóricos , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Reproducibilidad de los Resultados , Temperatura , Factores de Tiempo
5.
Se Pu ; 27(3): 379-81, 2009 May.
Artículo en Chino | MEDLINE | ID: mdl-19803151

RESUMEN

The removal of the colloidal impurities in the purification of salvianolic acid B (SB) was carried out on a macroporous adsorption resin column with a modified elution method on the principle of combining frontal chromatography with displacement chromatography. Two serially connected columns were packed with macroporous adsorption resin in a certain proportion. The latter column was eluted with 50% methanol, when the colloidal impurities reached saturation adsorption in the former column. Comparative study suggested that the SB product was superior to that from the routine method with the weighted average purity risen from 59.6% to 64.5%, and the recovery from 69.75% to 80.0%. The ethanol concentration was lowered in the eluent. The method is simple and can be used for the production of SB in large scale.


Asunto(s)
Benzofuranos/análisis , Benzofuranos/aislamiento & purificación , Cromatografía/métodos , Coloides/aislamiento & purificación , Tecnología Farmacéutica/métodos , Adsorción , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Fenantrolinas/química , Porosidad , Resinas Sintéticas/química , Salvia miltiorrhiza
6.
Water Sci Technol ; 52(10-11): 185-92, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-16459791

RESUMEN

This work presents a thorough fractionation of COD in raw sewage, followed by pilot plant coagulation tests with low-dosage lime (pH 9). Through a physical separation (sieving and crossflow filtration) total COD in the raw sewage was partitioned among eight size fractions in the range of 150-0.02 microm. In addition, respirometric tests were performed to measure the biodegradability of the different size fractions. More than 60% of COD was associated with settleable and supracolloidal particles (size > 1 microm), which are characterised by slow biodegradability. Coagulation with lime increased COD removal efficiencies in the primary treatment from typical 30-35%, up to 65-70%, suggesting that lime may induce the almost complete removal of the slowly settling, slowly biodegradable supracolloidal particles in the primary treatment. On the basis of these results a non-conventional sewage treatment scheme is proposed, considering that there is plenty of space for improving primary treatment efficiency through sewage coagulation. Higher primary treatment efficiency may present several advantages, including lower aeration energy in the subsequent biological unit and higher energy recovery from sludge digestion.


Asunto(s)
Compuestos de Calcio/farmacología , Óxidos/farmacología , Aguas del Alcantarillado/análisis , Eliminación de Residuos Líquidos/métodos , Contaminantes del Agua/aislamiento & purificación , Biodegradación Ambiental , Coloides/química , Coloides/aislamiento & purificación , Coloides/metabolismo , Filtración , Concentración de Iones de Hidrógeno , Oxígeno/química , Oxígeno/metabolismo , Tamaño de la Partícula , Aguas del Alcantarillado/química , Aguas del Alcantarillado/microbiología , Factores de Tiempo
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