RESUMEN
A new method involving gut microbiota biotransformation, spectrum-effect relationship analysis and metabolomics analysis was developed to study the antitussive and expectorant microbial metabolites of platycosides fraction (MPFs) of Platycodonis Radix. Furthermore, their possible metabolic mechanisms were studied for the first time. The findings showed that the antitussive and expectorant effects of the platycosides fraction (PF) were significantly enhanced by the gut microbiota biotransformation. 11 active antitussive microbial metabolites and 12 active expectorant microbial metabolites, which shared 8 components, were successfully screened out via spectrum-effect relationship analysis. The prototypes of the active microbial metabolites could be reversely traced according to the gut microbiota biotransformation pathways. It was found out that one platycoside could produce several active microbial metabolites and several different platycosides could produce the same active microbial metabolite. In addition, the metabolomics analysis showed that both the PF and its active microbial metabolites could regulate the same metabolomic pathways of Linoleic acid metabolism, Arachidonic acid metabolism and Glycerophospholipid metabolism to exert antitussive activity, and regulate the same metabolomic pathway of Arachidonic acid metabolism to exert expectorant activity. These findings suggested the microbial metabolites may be the active forms of the platycosides. Overall, the proposed approach was useful in screening the active microbial metabolites; this work explained the in vivo antitussive and expectorant metabolic mechanisms of multi-constituents, multi-targets and synergistic effects of PF of Platycodonis Radix.
Asunto(s)
Antitusígenos , Expectorantes , Metaboloma/efectos de los fármacos , Extractos Vegetales , Platycodon , Animales , Antitusígenos/química , Antitusígenos/farmacología , Cromatografía Liquida , Expectorantes/química , Expectorantes/farmacología , Microbioma Gastrointestinal , Metabolómica , Ratones , Ácido Oleanólico/análogos & derivados , Extractos Vegetales/química , Extractos Vegetales/farmacología , Platycodon/química , SaponinasRESUMEN
Naringenin (NRG) is a natural flavonoid compound abundantly present in citrus fruits and has the potential to treat respiratory disorders. However, the clinical therapeutic effect of NRG is limited by its low bioavailability due to poor solubility. To enhance the solubility, naringenin nanosuspensions (NRG-NSps) were prepared by applying tocopherol polyethylene glycol succinate (TPGS) as the nanocarrier via the media-milling method. The particle size, morphology, and drug-loading content of NRG-NSps were examined, and the stability was evaluated by detecting particle size changes in different physiological media. NRG-NSps exhibited a flaky appearance with a mean diameter of 216.9 nm, and the drug-loading content was 66.7%. NRG-NSps exhibited good storage stability and media stability. NRG-NSps presented a sustainable release profile, and the cumulative drug-release rate approached approximately 95% within 7 d. NRG-NSps improved the antitussive effect significantly compared with the original NRG, the cough frequency was decreased from 22 to 15 times, and the cough incubation period was prolonged from 85.3 to 121.6 s. Besides, NRG-NSps also enhanced expectorant effects significantly, and phenol red secretion was increased from 1.02 to 1.45 µg/mL. These results indicate that NRG-NSps could enhance the bioavailability of NRG significantly and possess a potential clinical application.
Asunto(s)
Antitusígenos , Expectorantes , Flavanonas/farmacología , Animales , Antitusígenos/síntesis química , Antitusígenos/química , Antitusígenos/farmacología , Antitusígenos/uso terapéutico , Disponibilidad Biológica , Tos/tratamiento farmacológico , Tos/patología , Modelos Animales de Enfermedad , Sistemas de Liberación de Medicamentos , Evaluación Preclínica de Medicamentos , Liberación de Fármacos , Expectorantes/síntesis química , Expectorantes/química , Expectorantes/farmacología , Expectorantes/uso terapéutico , Flavanonas/síntesis química , Flavanonas/química , Flavanonas/uso terapéutico , Ratones , Nanopartículas , Tamaño de la Partícula , Solubilidad , SuspensionesRESUMEN
This study aimed to improve the mucus permeating properties of self-emulsifying drug delivery systems (SEDDS) by anchoring lipidized bromelain, papain and trypsin using palmitoyl chloride. SEDDS containing enzyme-palmitate conjugates were characterized regarding droplet size and zeta potential. Their mucus permeating properties were evaluated by Transwell diffusion and rotating tube method using fluorescein diacetate (FDA) as marker. Degree of substitution of modified enzymes was 35.3%, 47.8% and 38.5% for bromelain-palmitate, papain-palmitate and trypsin-palmitate, respectively. SEDDS as control and SEDDS containing enzyme-palmitate conjugates displayed a droplet size less than 50nm and 180-312nm as well as a zeta potential of -3 to -4 and -4 to -5mV, respectively. The highest percentage of permeation was achieved by introducing 5% papain-palmitate into SEDDS. It could enhance the mucus permeation of SEDDS in porcine intestinal mucus 4.6-fold and 2-fold as evaluated by Transwell diffusion and rotating tube method, respectively. It is concluded that mucus permeation of SEDDS can be strongly improved by incorporation of enzyme-palmitate conjugates.
Asunto(s)
Sistemas de Liberación de Medicamentos/métodos , Emulsionantes/administración & dosificación , Expectorantes/metabolismo , Moco/metabolismo , Animales , Bromelaínas/química , Bromelaínas/metabolismo , Emulsionantes/química , Emulsionantes/farmacocinética , Expectorantes/química , Mucosa Intestinal/metabolismo , Lípidos/química , Palmitatos/metabolismo , Papaína/química , Papaína/metabolismo , Tamaño de la Partícula , Permeabilidad , Porcinos , Tripsina/química , Tripsina/metabolismoRESUMEN
Licorice has been used as an antitussive and expectorant herbal medicine for a long history. This work evaluated the activities of 14 major compounds and crude extracts of licorice, using the classical ammonia-induced cough model and phenol red secretion model in mice. Liquiritin apioside (1), liquiritin (2), and liquiritigenin (3) at 50â¯mg/kg (i.g.) could significantly decrease cough frequency by 30-78% (pâ¯<â¯.01). The antitussive effects could be partially antagonized by the pretreatment of methysergide or glibenclamide, but not naloxone. Moreover, compounds 1-3 showed potent expectorant activities after 3â¯days treatment (pâ¯<â¯.05). The water and ethanol extracts of licorice, which contain abundant 1 and 2, could decrease cough frequency at 200â¯mg/kg by 25-59% (pâ¯<â¯.05), and enhance the phenol red secretion (pâ¯<â¯.05), while the ethyl acetate extract showed little effect. These results indicate liquiritin apioside and liquiritin are the major antitussive and expectorant compounds of licorice. Their antitussive effects depend on both peripheral and central mechanisms.
Asunto(s)
Antitusígenos/farmacología , Tos/tratamiento farmacológico , Expectorantes/farmacología , Glycyrrhiza/química , Fenolsulfonftaleína/metabolismo , Extractos Vegetales/farmacología , Administración Oral , Amoníaco , Animales , Antitusígenos/antagonistas & inhibidores , Antitusígenos/química , Tos/inducido químicamente , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Expectorantes/química , Expectorantes/aislamiento & purificación , Gliburida/farmacología , Masculino , Metisergida/farmacología , Ratones , Ratones Endogámicos ICR , Estructura Molecular , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Relación Estructura-ActividadRESUMEN
OBJECTIVE: This study aimed to design and characterize an inhalable dry powder of ciprofloxacin or levofloxacin combined with the mucolytics acetylcysteine and dornase alfa for the management of pulmonary infections in patients with cystic fibrosis. METHODS: Ball milling, homogenization in isopropyl alcohol and spray drying processes were used to prepare dry powders for inhalation. Physico-chemical characteristics of the dry powders were assessed via thermogravimetric analysis, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry and scanning electron microscopy. The particle size distribution, dissolution rate and permeability across Calu-3 cell monolayers were analyzed. The aerodynamic parameters of dry powders were determined using the Andersen cascade impactor (ACI). RESULTS: After the micronization process, the particle sizes of the raw materials significantly decreased. X-ray and DSC results indicated that although ciprofloxacin showed no changes in its crystal structure, the structure of levofloxacin became amorphous after the micronization process. FT-IR spectra exhibited the characteristic peaks for ciprofloxacin and levofloxacin in all formulations. The dissolution rates of micro-homogenized and spray-dried ciprofloxacin were higher than that of untreated ciprofloxacin. ACI results showed that all formulations had a mass median aerodynamic diameter less than 5 µm; however, levofloxacin microparticles showed higher respirability than ciprofloxacin powders did. The permeability of levofloxacin was higher than those of the ciprofloxacin formulations. CONCLUSION: Together, our study showed that these methods could suitably characterize antibiotic and mucolytic-containing dry powder inhalers.
Asunto(s)
Ciprofloxacina/administración & dosificación , Ciprofloxacina/uso terapéutico , Fibrosis Quística/tratamiento farmacológico , Desoxirribonucleasa I/química , Expectorantes/química , Levofloxacino/administración & dosificación , Levofloxacino/uso terapéutico , Polvos/administración & dosificación , Administración por Inhalación , Rastreo Diferencial de Calorimetría , Química Farmacéutica , Ciprofloxacina/química , Fibrosis Quística/fisiopatología , Desoxirribonucleasa I/administración & dosificación , Inhaladores de Polvo Seco , Expectorantes/farmacocinética , Humanos , Levofloxacino/química , Microscopía Electrónica de Rastreo , Tamaño de la Partícula , Polvos/química , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
As aplicações da biodiversidade brasileira em saúde são abrangentes, mas ainda não têm sido bem exploradas oficialmente, considerando o pequeno número de espécies vegetais listadas em compêndios oficiais para uso medicinal. Das espécies conhecidas como assa-peixe, empregadas comumente pela população há muitos anos para diversos fins, sobretudo nas afecções respiratórias, Vernonia polyanthes (Spreng.) Less. é a única regulamentada para utilização no Brasil, indicada como expectorante no Formulário de Fitoterápicos da Farmacopeia Brasileira. Seu cultivo é também praticado em Arranjos Produtivos Locais (APLs) de plantas medicinais, os quais possuem importante papel na conservação e sustentabilidade dos recursos naturais e na economia das famílias que dependem dessa parceria. Esta difusão de uso é característica de diversas espécies que compõem a Relação Nacional de Plantas Medicinais de Interesse ao SUS, mas ainda sem monografias que respaldem os critérios de qualidade para seu uso seguro, conforme o preconizado pela Organização Mundial de Saúde para plantas medicinais. O objetivo deste trabalho foi apresentar uma visão geral do conhecimento científico desta espécie, com foco em sua utilização na fitoterapia, identificação química e atividades farmacológicas, na expectativa de subsidiar a elaboração de monografias de Vernonia polyanthes, agregando valor e qualidade nas atividades dos APLs a longo prazo.(AU)
The applications of the Brazilian biodiversity in health are comprehensive, but still officially underused, since the number of medicinal plants listed in official compendia is minimal. Of several species popularly known as assa-peixe, which have been in common use by the population for many years with diverse pharmacological actions, particularly that related to respiratory affections, Vernonia polyanthes (Spreng.) Less. is the only one listed for use by Herbal Formulary of the Brazilian Pharmacopoeia, indicated as expectorant. It is also cultivated by Local Productive Arrangements (LPA) of medicinal plants, which have an important role in the conservation and sustainability of natural resources, as well as for the economic status of families that depend on this partnership. Several species widely used in folk medicine that compose the National List of Medicinal Plants of Interest to Unified Health System have no monograph that support the quality criteria for the safe use of medicinal plants, following the recommendations of World Health Organization. The present study aims at providing an overview of the scientific knowledge about this species, focusing on its use in herbal medicine, phytochemical identification, and pharmacological activities. This overview can help the elaboration of monographs of Vernonia polyanthes, adding value and quality in the LPA activities at long term.(AU)
Asunto(s)
Humanos , Expectorantes/química , Fitoterapia , Vernonia/químicaRESUMEN
Descurainia sophia is widely distributed in China and is one of the most troublesome annual weeds. It has diverse medicinal usage. D. sophia has abundant oil, making it an important oil plant in China. The main goal of this study was to obtain the maximum yield of the oil by an optimal selection of supercritical fluid extraction parameters. According to the central composite design and response surface methodology for supercritical fluid extraction method, a quadratic polynomial model was used to predict the yield of D. sophia seed oil. A series of runs was performed to assess the optimal extraction conditions. The results indicated that the extraction pressure had the greatest impact on oil yield within the range of the operating conditions studied. A total of approximately 67 compounds were separated in D. sophia seed oil by GC-MS, of which 51 compounds represented 98.21% of the total oils, for the first time. This study was also aimed at evaluating the anti-asthmatic, anti-tussive and expectorant activities in vivo of D. sophia seed oil which supplied for further research on bioactive constituents and pharmacological mechanisms.
Asunto(s)
Antiasmáticos , Antitusígenos , Brassicaceae/química , Expectorantes , Aceites de Plantas/química , Semillas/química , Animales , Antiasmáticos/química , Antiasmáticos/aislamiento & purificación , Antiasmáticos/farmacología , Antitusígenos/química , Antitusígenos/aislamiento & purificación , Antitusígenos/farmacología , Evaluación Preclínica de Medicamentos , Expectorantes/química , Expectorantes/aislamiento & purificación , Expectorantes/farmacología , Femenino , Cobayas , Masculino , RatonesRESUMEN
Verticinone, the main active component in F. hupehensis, exhibits potent antitussive and expectorant effects. Here, a LC-MS method was developed and applied to study the pharmacokinetics, tissue distribution and excretion of verticinone in rats, and its plasma protein binding in vitro. A significant gender difference in the pharmacokinetics of verticinone in rats was observed, as its absolute oral bioavailability in male and female rats was 45.8% and 2.74%, respectively. The relative bioavailability of verticinone was significantly lower in female rats as compared to male, following intragastrical (i.g.) and intravenous (i.v.) administration. After successive i.g. administration of verticinone, accumulation was observed in female rats but not in the male ones. The tissue distribution study showed that verticinone had a good tissue penetrability and a high tissue affinity in most studied tissues, except brain. After a 2 mg/kg oral dose, less than 4% of the dose was excreted as unchanged parent compound in male rats, and less than 1% in female rats, which indicated that verticinone was metabolized more extensively in female rats than in male rats.
Asunto(s)
Cevanas/farmacocinética , Expectorantes/farmacocinética , Fritillaria/química , Extractos Vegetales/farmacocinética , Animales , Proteínas Sanguíneas/química , Cevanas/administración & dosificación , Cevanas/química , Evaluación Preclínica de Medicamentos , Expectorantes/administración & dosificación , Expectorantes/química , Femenino , Humanos , Masculino , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Ratas Sprague-Dawley , Distribución TisularRESUMEN
Three alkaloids, (3-chloro-2-hydroxypropyl) trimethylammonium chloride (1), p-(acetylamino)-phenol (2) and 4,4'-diacetamidodiphenyl ether (3), were isolated from Reineckia carnea herba. Their structures were determined by detailed analysis of their 1D and 2D NMR spectra and MS. Compounds 1 and 3 were new natural products. Compound 2 was isolated for the first time from the Reineckia genus. Compound 1 displayed significant in vivo antitussive and expectorant activities.
Asunto(s)
Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Aminofenoles/aislamiento & purificación , Aminofenoles/farmacología , Antitusígenos/aislamiento & purificación , Antitusígenos/farmacología , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Expectorantes/aislamiento & purificación , Expectorantes/farmacología , Liliaceae/química , Éteres Fenílicos/aislamiento & purificación , Éteres Fenílicos/farmacología , Propanoles/aislamiento & purificación , Propanoles/farmacología , Compuestos de Amonio Cuaternario/aislamiento & purificación , Compuestos de Amonio Cuaternario/farmacología , Alcaloides/química , Aminofenoles/química , Antitusígenos/química , Tos/tratamiento farmacológico , Medicamentos Herbarios Chinos/química , Expectorantes/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Éteres Fenílicos/química , Raíces de Plantas/química , Propanoles/química , Compuestos de Amonio Cuaternario/químicaRESUMEN
OBJECTIVE: To research the expectorant components in volatile oil from the root and rhizome of Aster tataricus. METHOD: GC-MS was applied to isolate and identify the compounds. In addition, TLC was used to isolate compound, and its structure was elucidated on the basis of spectral data analysis. At the same time, its expectorant effect was observed by method of the excretion quantity of phenol red in trachea of mice. RESULT: Seven compounds were isolated and identified by GC-MS, they were (R)(-)-p-menth-1-en-4-ol (1), 2-undecanone (2), n-decanoic acid (3), (-)-spathulenol (4), hexahydrofamrnesyl acetone (5), hexadecanoic acid (6), and cis-9, cis-12-octaecadienoic acid (7). A known compound 1-acetoxy-2-ene(E)-4,6- decandiyne was isolated from the root and rhizome of A. tataricus, and it was shown to have expectorant effect. CONCLUSION: 1-Acetoxy-2-ene(E) -4,6- decandiyne, a main compound in volatile oil, had been found to have expectorant effect.
Asunto(s)
Aster/química , Medicamentos Herbarios Chinos/química , Expectorantes/química , Aceites Volátiles/química , Raíces de Plantas/química , Rizoma/química , Animales , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Expectorantes/aislamiento & purificación , Expectorantes/farmacología , Cromatografía de Gases y Espectrometría de Masas , Espectroscopía de Resonancia Magnética , Masculino , RatonesRESUMEN
To search for potential drugs with potent antitussive, expectorant, antiasthmatic activities and low toxicity, a series of verticinone-bile acids salts were prepared based on the clearly elucidated antitussive, expectorant and antiasthmatic activities of verticinone in bulbs of Fritillaria and different bile acids in Snake Bile. The antitussive, expectorant and antiasthmatic activities of these verticinone-bile acid salts were then screened with different animal models. Ver-CA (verticinone-cholic acid salt) and Ver-CDCA (verticinone-chenodeoxycholic acid salt) showed much more potent activities than other compounds. The bioactivities of Ver-CA and Ver-CDCA are worthy to be intensively studied, and it is also deserved to pay much attention to their much more potent antitussive effects than codeine phosphate. In order to elucidate whether they have synergistic effect and attenuated toxicity, their activities will be continuously compared with single verticinone, cholic acid and chenodeoxycholic acid at the same doses on different animal models. The application of "combination principles" in traditional Chinese medicinal formulations may be a novel way in triditional Chinese medicine research and discovery.
Asunto(s)
Antiasmáticos/farmacología , Antitusígenos/farmacología , Ácidos y Sales Biliares/farmacología , Cevanas/farmacología , Expectorantes/farmacología , Animales , Antiasmáticos/química , Antitusígenos/química , Asma/prevención & control , Ácidos y Sales Biliares/química , Cevanas/química , Cevanas/aislamiento & purificación , Ácido Quenodesoxicólico/química , Ácido Quenodesoxicólico/farmacología , Ácido Cólico/química , Ácido Cólico/farmacología , Tos/prevención & control , Combinación de Medicamentos , Composición de Medicamentos/métodos , Sinergismo Farmacológico , Expectorantes/química , Femenino , Fritillaria/química , Cobayas , Masculino , Ratones , Plantas Medicinales/química , Distribución Aleatoria , SerpientesRESUMEN
OBJECTIVE: To establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC. METHOD: A C18 column with a solvent system of acetonitrile-0.02 mol x L(-1) sodium dihydrogen phosphate (0.2% triethylamine, phosphoric acid, at pH 3) (25:75) and UV detection 240 nm were used. The flow rate was 1.0 mL x min(-1). The column temperature was maintained at 40 degrees C. RESULT: There was a good linear relationship between the absorption value and the concentration in the range of 0.020 2-0.100 5 microg for papaverine. The average recovery rates were 99.1% (RSD 2.3%). CONCLUSION: The method is simple, accurate and can be used to determine the contents in Qiangli Pibalu.
Asunto(s)
Medicamentos Herbarios Chinos/química , Papaverina/análisis , Plantas Medicinales , Antitusígenos/química , Antitusígenos/farmacología , Cromatografía Líquida de Alta Presión/métodos , Combinación de Medicamentos , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Eriobotrya/química , Expectorantes/química , Expectorantes/farmacología , Papaver/química , Plantas Medicinales/química , Control de Calidad , Reproducibilidad de los ResultadosRESUMEN
Gels formed in situ following oral administration of dilute aqueous solutions of pectin (1.0 and 1.5%, w/v) to rats were evaluated as vehicles for the sustained release of the expectorant drug ambroxol hydrochloride. The solutions contained calcium ions in complexed form, which on release in the acidic environment of the stomach caused gelation of the pectin. In vitro studies demonstrated diffusion-controlled release of ambroxol from the gels over a period of 6 h. A bioavailability of ambroxol of approximately 64% of that of a commercially available formulation could be achieved from gels containing an identical dose of ambroxol formed in situ in the stomachs of rats, with appreciably lower peak plasma levels and a sustained release of drug over a period of at least 6 h. The influence of added sorbitol (17%, w/v) on the rheological and drug release properties of the formulations has been examined.
Asunto(s)
Ambroxol/administración & dosificación , Ambroxol/química , Expectorantes/administración & dosificación , Expectorantes/química , Pectinas/química , Ambroxol/farmacocinética , Animales , Área Bajo la Curva , Disponibilidad Biológica , Calcio/química , Cromatografía Líquida de Alta Presión , Preparaciones de Acción Retardada , Expectorantes/farmacocinética , Geles , Masculino , Ratas , Ratas Wistar , Solubilidad , Sorbitol/química , ViscosidadRESUMEN
This multicenter, placebo-controlled, double-blind, randomized parallel-group trial was conducted to investigate the efficacy and tolerability of myrtol standardized (MYS, Gelomyrtol forte, 3 x 300 mg) in the long-term treatment of patients with chronic bronchitis during the winter. 246 patients received the investigational treatments (MYS: 122, placebo: 124) for at least 1 month; 215 subjects (110 under MYS and 105 under placebo) were evaluable in terms of efficacy (exacerbation rate, the need for antibiotics, symptom scores and general well-being) for the protocol-defined 6 months of treatment. Statistically significantly (p < 0.01) more patients remained without acute exacerbation in the myrtol standardized group (72%) compared to the placebo group (53%). In the placebo group, there was an evident peak in the incidence of exacerbations during the third month of treatment, which was not observed in the active treatment group. In the MYS group, 51.6% of the patients with an acute exacerbation required antibiotics vs. 61.2% under placebo. 62.5% of the patients treated with antibiotics in the MYS group required them for < or = 7 days, whereas 76.7% of the patients in the placebo group treated with antibiotics for exacerbation needed antibiotics for > 7 days. Well-being (assessed in terms of general health and health impairment by cough and expectoration) was significantly better under treatment with MYS. The overall therapeutic efficacy evaluation scored higher for MYS. Therefore, it is concluded that long-term treatment with MYS is equally well tolerated as placebo but is clearly superior in efficacy in terms of protecting against acute exacerbations in patients with chronic bronchitis: it reduces the frequency and intensity of acute exacerbations, the need of antibiotics for them and the health impairment by cough and expectoration.
Asunto(s)
Bronquitis/tratamiento farmacológico , Expectorantes/uso terapéutico , Monoterpenos , Terpenos/uso terapéutico , Anciano , Antibacterianos/uso terapéutico , Bronquitis/fisiopatología , Enfermedad Crónica , Método Doble Ciego , Combinación de Medicamentos , Expectorantes/efectos adversos , Expectorantes/química , Femenino , Volumen Espiratorio Forzado , Humanos , Masculino , Persona de Mediana Edad , Terpenos/efectos adversos , Terpenos/químicaRESUMEN
An efficient method combining SEP-PAK C18 cartridge solid phase extraction (SPE) with reversed-phase high performance liquid chromatography for the quantitation of morphine in the Compound Liquorice Tablet is presented. The tablets have been used for making expectoration easy and relieving cough for years. The tablet powder (approximately 1 tablet) was added into a stoppered centrifuge tube, and vertically extracted with 5 mL of 0.5% HAc for 5 min. After centrifugation the supernatant was transferred to a beaker and the extraction was repeated twice with 4 mL and then 3 mL of 0.5% HAc. Five millilitres of carbonate buffer (pH 8.9) was added to the combined extracts. A SEP-PAK C18 cartridge was pretreated by passing methanol and distilled water, using a glass syringe. The mixture was applied on it and allowed to flow through. The cartridge was washed first with 5 mL of 10% methanol solution and then the morphine was eluted with 4 mL of 70% methanol solution into a 5 mL volumetric flask and was finally diluted to volume with 70% methanol solution. Analysis was performed on a mu-Bondapak C18 column(300 mm x 4.6 mm i.d., 10 microns) with 0.1 mol/L NaH2PO4-methanol(5:1) as the mobile phase and detection at 286 nm. The average recovery of morphine was (101.2 +/- 1.5)% and RSD was 1.5%. The method is simple, rapid, accurate, and reproducible, and can be used in drug control.
Asunto(s)
Antitusígenos/química , Medicamentos Herbarios Chinos/química , Expectorantes/química , Morfina/análisis , Composición de Medicamentos , ComprimidosRESUMEN
A new steroidal alkaloidal glucoside, C34H57NO7, mp 284-286 degrees C, named ningpeisinoside, was isolated from the bulb of Fritillaria ningguoensis S.C. Chen et S.F. Yin. Based on preparation of derivatives and IR, MS, 1HNMR, 13CNMR spectral studies, the structure of ningpeisinoside has been established as N-methyl-5 alpha-veratranine-6-oxo-3 beta-O-beta-D-glucoside(I).