RESUMEN
Seven previously undescribed trichothecenes, named trichothecrotocins M-S (1-7), along with five known compounds, were isolated from rice cultures of the potato-associated fungus Trichothecium crotocinigenum. Their structures and absolute configurations were determined through spectroscopic methods, single-crystal X-ray diffraction, and quantum chemistry calculations on ECD. Compound 1 possesses a rare 6,11-epoxy moiety in the trichothecene family. Compound 6 exhibited strong cytotoxic activity against MCF-7 cancer cell lines with an IC50 value of 2.34 ± 0.45 µM. It promoted apoptosis induction in MCF-7 cells. Moreover, cell cycle analysis showed cell cycle arrest caused by compound 6 at the G2/M phase which resulted to cell proliferation inhibition and pro-apoptotic activity. Further quantitative real-time PCR (qRT-PCR) analysis confirmed that the G2/M arrest was accompanied by upregulation of p21 and down regulation of cyclins B1 in 6-treated MCF-7 cells.
Asunto(s)
Antineoplásicos/farmacología , Hypocreales/química , Solanum tuberosum/química , Tricotecenos/farmacología , Antineoplásicos/química , Antineoplásicos/metabolismo , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Hypocreales/metabolismo , Células MCF-7 , Simulación del Acoplamiento Molecular , Estructura Molecular , Solanum tuberosum/metabolismo , Relación Estructura-Actividad , Tricotecenos/química , Tricotecenos/metabolismoRESUMEN
Agricultural commodities, particularly cereals can be contaminated with mycotoxins during the pre- and post-harvest stage. The main goal of this study was to evaluate the efficacy of magnetic zeolite nanocomposite (MZNC) as an adsorbent for the reduction of mycotoxins in barley flour. The MZNC is synthesised using an eco-friendly and efficient procedure and characterised by zeta potential, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The adsorbent amount that affects the adsorption capacity was optimised. Low amounts of the nanocomposite removed >99% of aflatoxins, 50% of ochratoxin A, 22% of zearalenone, and 1.8% of the deoxynivalenol from the contaminated sample and adsorption by MZNC was better than the natural zeolite; this phenomenon is related to the wide surface of nanocomposites. Results provide new insights into possible future research that could overcome the challenges of using nanotechnology to eliminate mycotoxins from agricultural products. It can be hoped that the presence of cheap and eco-friendly mycotoxin binders such as the MZNC that is synthesised and utilised in this research will help to produce secure food and feed products.
Asunto(s)
Aflatoxinas/química , Hordeum/efectos de los fármacos , Micotoxinas/química , Nanotecnología/métodos , Ocratoxinas/química , Tricotecenos/química , Zearalenona/química , Adsorción , Centaurea , Grano Comestible/química , Fenómenos Magnéticos , Microscopía Electrónica de Rastreo , Extractos Vegetales/química , Polvos , ZeolitasRESUMEN
The effect of fermented wheat germ extract (FWGE) (Immunovet®) was evaluated with cotreatments with deoxynivalenol (DON) and T-2 toxin (T-2). These mycotoxins are produced by Fusarium mold species. The effects of FWGE on IPEC-J2 with DON and T-2 have not been studied until now. The IPEC-J2 porcine, nontumorigenic cell line was selected to investigate the outcome of the individually and simultaneously added compounds, as it has in vivo-like properties. The cells were treated for 24 h with the selected solutions; then, the IPEC-J2 cells were allowed to regenerate in a culture medium for an additional 24 h. In our results, DON and T-2 significantly increased the adverse impacts on cell viability and integrity of the cell monolayer. To elucidate the extent of oxidative stress, extracellular H2O2 concentrations and intracellular reactive oxygen species (ROS) were measured. FWGE appeared to be beneficial to IPEC-J2 cells given the separately and significantly decreased ROS levels. 1% and 2% FWGE could significantly reduce mycotoxin-induced oxidative stress. In conclusion, the results demonstrate that FWGE exerted protective effects to counteract the oxidative stress-provoking properties of applied fusariotoxins in the nontumorigenic IPEC-J2 cell line.
Asunto(s)
Células Epiteliales/efectos de los fármacos , Micotoxinas/toxicidad , Extractos Vegetales/farmacología , Tricotecenos/toxicidad , Animales , Línea Celular , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Supervivencia Celular/efectos de los fármacos , Impedancia Eléctrica , Células Epiteliales/metabolismo , Fluoresceínas/metabolismo , Espacio Intracelular/metabolismo , Micotoxinas/química , Especies Reactivas de Oxígeno/metabolismo , Porcinos , Tricotecenos/químicaRESUMEN
Fusarium rot of muskmelon is a common and frequently-occurring postharvest disease, which leads to quality deterioration and neosolaniol (NEO) contamination. New strategies to control postharvest decay and reduce NEO contamination are of paramount importance. The effects of acetylsalicylic acid (ASA) treatment on the growth of Fusarium sulphureum in vitro, and Fusarium rot development and NEO accumulation in fruits inoculated with F. sulphureum in vivo were investigated. The results showed that ASA inhibited the growth of F. sulphureum, evident morphological and major cellular changes were observed under the microscope. In vivo testing showed that 3.2â¯mg/mL ASA significantly suppressed Fusarium rot development and NEO accumulation after 6 and 8â¯d of pathogen inoculation. Meanwhile, Tri gene expressions involved in NEO biosynthesis were down-regulated after treatment. Taken together, ASA treatment not only reduced Fusarium rot development by inhibiting the growth of F. sulphureum, but decreased NEO accumulation by suppressing NEO biosynthesis pathway.
Asunto(s)
Aspirina/farmacología , Cucurbitaceae/química , Tricotecenos/metabolismo , Cucurbitaceae/metabolismo , Cucurbitaceae/microbiología , Frutas/química , Frutas/metabolismo , Frutas/microbiología , Fusarium/efectos de los fármacos , Fusarium/crecimiento & desarrollo , Fusarium/metabolismo , Fusarium/ultraestructura , Enfermedades de las Plantas/prevención & control , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , ARN de Planta/genética , ARN de Planta/metabolismo , Tricotecenos/químicaRESUMEN
Chromatographic separation of extracts from the fungal biomass of a plant pathogenic fungus, Myrothecium roridum, yielded 8 trichothecene toxins including 6 type D trichothecenes (1: -6: ) and 2 type A trichothecenes (7: -8: ). 6',12'-Epoxymyrotoxin A (1: ) and 7'-hydroxymytoxin B (2: ) were new macrocyclic trichothecenes, while the other trichothecenes were identified as myrotoxin B (3: ), myrotoxin D hydrate (4: ), 2',3'-epoxymyrothecine A (5: ), miotoxin A (6: ), and 2 trichothecenes lacking the macrocyclic lactone system, roridin L-2 (7: ) and trichoverritone (8: ). The structures of these mycotoxins were characterized using spectroscopic methods. The absolute configurations of 1: and 2: were determined by NOESY and a comparison of their experimental and calculated ECD spectra. Most of these mycotoxins (1: -4: and 6: ) exhibited highly potent antimalarial activity against Plasmodium falciparum. They also showed strong cytotoxicity towards KB and NCI-H187 cell lines (IC50 0.60â-â112.28 nM), as well as the Vero cell line (IC50 1.50â-â46.51 nM).
Asunto(s)
Antimaláricos/farmacología , Antineoplásicos/farmacología , Hypocreales/química , Micotoxinas/farmacología , Tricotecenos/química , Animales , Antimaláricos/química , Antineoplásicos/química , Línea Celular Tumoral , Chlorocebus aethiops , Evaluación Preclínica de Medicamentos , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Estructura Molecular , Micotoxinas/química , Plasmodium falciparum/efectos de los fármacos , Relación Estructura-Actividad , Tricotecenos/farmacología , Células VeroRESUMEN
Six new (1-6), together with seven known (7-13), trichothecenes were isolated from the soil-derived Trichoderma brevicompactum PSU-RSPG27. Their structures were established using spectroscopic data. The structure of 1 was confirmed by X-ray data. Trichodermin (7) exhibited the most potent activity against Plasmodium falciparum (K1 strain) with an IC50 value of 0.1 µM, while other trichothecenes (1, 8, 9, and 12) were much less active, with IC50 values in the range of 7.1-9.6 µM. Compound 7 displayed activity against noncancerous Vero cells with an IC50 value of 0.4 µM. The remaining compounds showed moderate to weak activity, with IC50 values in the range of 6.9-15.3 µM. Compounds 7 and 12 were active against human oral carcinoma (KB) cells with IC50 values of 2.4 and 3.7 µM, respectively. Additionally, compounds 7 and 12 displayed antifungal activity against Candida albicans with the respective MIC values of 1 and 2 µg/mL and were active against Cryptococcus neoformans with equal MIC values of 4 µg/mL.
Asunto(s)
Microbiología del Suelo , Trichoderma/química , Tricotecenos/aislamiento & purificación , Espectroscopía de Resonancia Magnética con Carbono-13 , Línea Celular Tumoral , Evaluación Preclínica de Medicamentos , Humanos , Concentración 50 Inhibidora , Pruebas de Sensibilidad Microbiana , Plasmodium falciparum/efectos de los fármacos , Espectroscopía de Protones por Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray , Tricotecenos/química , Tricotecenos/farmacologíaRESUMEN
Animal feed is prone to becoming infected with molds during production and storage, resulting in secondary metabolite mycotoxins, such as aflatoxin B1 (AFB1), T-2 toxins, deoxynivalenol (DON), and ochratoxin A (OTA), which are harmful to humans and animals. Selenium is an essential trace element for humans and animals, and it is also an effective antioxidant. Many studies have shown that selenium can reduce the damage caused by mycotoxins in animals. This article reviews the current literature on the antagonistic effects of selenium on AFB1, T-2, DON, and OTA toxicity.
Asunto(s)
Alimentación Animal/análisis , Micotoxinas/química , Selenio/química , Aflatoxina B1/química , Ocratoxinas/química , Tricotecenos/químicaRESUMEN
Five new botryane sesquiterpenes (1: -5: ), one new cyclopentadepsipeptide (9: ), and two new xanthones (11: â-â12: ), together with 11 known compounds, were isolated from Trichoderma oligosporum. The structures of the new compounds were identified by comprehensive spectroscopic methods including nuclear magnetic resonance and mass spectrometry. The cytotoxicity of 1: -19: was evaluated against K562, A549, and ASPC cell lines. Compounds 5, 8, 11, 17: , and 18: showed cytotoxicity against the K562 cell line with more than 50% inhibition at 12.5 µM. As to A549 cell line, compound 8: showed the strongest cytotoxicity with approximately 50% inhibition at 25.0 µM. No compounds showed cytotoxicity against the ASPC cell line.
Asunto(s)
Péptidos Cíclicos/análisis , Terpenos/análisis , Trichoderma/química , Tricotecenos/análisis , Xantonas/análisis , Células A549/efectos de los fármacos , Antibacterianos/farmacología , Antineoplásicos/farmacología , Cristalografía por Rayos X , Escherichia coli/efectos de los fármacos , Humanos , Células K562/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Péptidos Cíclicos/química , Péptidos Cíclicos/aislamiento & purificación , Péptidos Cíclicos/farmacología , Staphylococcus aureus/efectos de los fármacos , Terpenos/química , Terpenos/aislamiento & purificación , Terpenos/farmacología , Tricotecenos/química , Tricotecenos/aislamiento & purificación , Tricotecenos/farmacología , Xantonas/química , Xantonas/aislamiento & purificación , Xantonas/farmacologíaRESUMEN
Zeolites are often used as adsorbents materials and their loaded cations can be exchanged with metal ions in order to add antimicrobial properties. The aim of this study was to use the 4A zeolite and its derived ion-exchanged forms with Zn2+, Li+, Cu2+ and Co2+ in order to evaluate their antifungal properties against Fusarium graminearum, including their capacity in terms of metal ions release, conidia germination and the deoxynivalenol (DON) adsorption. The zeolites ion-exchanged with Li+, Cu2+, and Co2+ showed an excellent antifungal activity against F. graminearum, using an agar diffusion method, with a zone of inhibition observed around the samples of 45.3 ± 0.6 mm, 25.7 ± 1.5 mm, and 24.7 ± 0.6 mm, respectively. Similar results using agar dilution method were found showing significant growth inhibition of F. graminearum for ion-exchanged zeolites with Zn2+, Li+, Cu2+, and Co2+. The fungi growth inhibition decreased as zeolite-Cu2+>zeolite-Li+>zeolite-Co2+>zeolite-Zn2+. In addition, the conidia germination was strongly affected by ion-exchanged zeolites. With regard to adsorption capacity, results indicate that only zeolite-Li+ were capable of DON adsorption significantly (P < 0.001) with 37% at 2 mg mL-1 concentration. The antifungal effects of the ion-exchanged zeolites can be ascribed to the interactions of the metal ions released from the zeolite structure, especially for zeolite-Li+, which showed to be a promising agent against F. graminearum and its toxin.
Asunto(s)
Fungicidas Industriales/farmacología , Fusarium/efectos de los fármacos , Tricotecenos/química , Zeolitas/química , Zeolitas/farmacología , Adsorción , Evaluación Preclínica de Medicamentos/métodos , Fungicidas Industriales/química , Fusarium/crecimiento & desarrollo , Litio/química , Litio/farmacología , Metales/químicaRESUMEN
Trichothecene mycotoxins are a type of sesquiterpenoid produced by various kinds of plantpathogenic fungi. In this study, two trichothecene toxins, namely, a novel cytotoxic epiroridin acid and a known trichothecene, mytoxin B, were isolated from the endophytic fungus Myrothecium roridum derived from the medicinal plant Pogostemon cablin. The two trichothecene mytoxins were confirmed to induce the apoptosis of HepG-2 cells by cytomorphology inspection, DNA fragmentation detection, and flow cytometry assay. The cytotoxic mechanisms of the two mycotoxins were investigated by quantitative real time polymerase chain reaction, western blot, and detection of mitochondrial membrane potential. The results showed that the two trichothecene mycotoxins induced the apoptosis of cancer cell HepG-2 via activation of caspase-9 and caspase-3, up-regulation of bax gene expression, down-regulation of bcl-2 gene expression, and disruption of the mitochondrial membrane potential of the HepG-2 cell. This study is the first to report on the cytotoxic mechanism of trichothecene mycotoxins from M. roridum. This study provides new clues for the development of attenuated trichothecene toxins in future treatment of liver cancer.
Asunto(s)
Apoptosis/efectos de los fármacos , Hypocreales/química , Micotoxinas/administración & dosificación , Tricotecenos/administración & dosificación , Caspasas/biosíntesis , Proliferación Celular/efectos de los fármacos , Fragmentación del ADN/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Células Hep G2 , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Micotoxinas/química , Neoplasias/tratamiento farmacológico , Pogostemon/microbiología , Sesquiterpenos/administración & dosificación , Sesquiterpenos/química , Tricotecenos/químicaRESUMEN
A new cytotoxic roridin-type trichothecene macrolide named epiroridin acid (1) and two known compounds epiroridin E (2) and mytoxin B (3) were isolated from the liquid culture of Myrothecium roridum A553, which was isolated from the medicinal plant Pogostemon cablin. The structure of the new macrolide (1) was elucidated by extensive spectroscopic measurements (UV, IR, MS, and 1D and 2D NMR) analyses. All isolated compounds (1-3) were evaluated for their cytotoxic activities against SF-268, MCF-7, NCI-H460, and HepG-2 tumor cell lines. The new compound (1) exhibited well cytotoxicity against the four selected tumor cell lines.
Asunto(s)
Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Hypocreales/química , Macrólidos/aislamiento & purificación , Macrólidos/farmacología , Tricotecenos/aislamiento & purificación , Tricotecenos/farmacología , Antibacterianos , Antineoplásicos/química , Línea Celular Tumoral , Endófitos , Humanos , Macrólidos/química , Estructura Molecular , Tricotecenos/químicaRESUMEN
The cytotoxicity of the extract obtained from Myrothecium roridum M10 and a characteristic (1)H signal at δH â¼8 led to the assumption that verrucarin/roridin-type compounds were present. Upscaling on rice medium led to the isolation of four new metabolites: verrucarins Y (1) and Z (6) (macrocyclic trichothecenes), bilain D (12) (a diketopiperazine derivative), and hamavellone C (14) (an unusual cyclopropyl diketone). In addition, nine known trichothecenes [verrucarin A (3), 16-hydroxyverrucarin A (5), verrucarin B (7), 16-hydroxyverrucarin B (8), verrucarin J (2), verrucarin X (4), roridin A (9), roridin L-2 (10), and trichoverritone (11)] and a bicyclic lactone [myrotheciumone A (15)] were identified. Their structures and configurations were determined by spectroscopic methods, published data, Mosher's method, and considering biosyntheses. Some trichothecenes showed motility inhibition followed by lysis of the zoospores against devastating Phytophthora nicotianae within 5 min. Compounds 2, 3, 7, and 9 also exhibited potent activities against Candida albicans and Mucor miehei.
Asunto(s)
Hypocreales/química , Phytophthora/efectos de los fármacos , Extractos Vegetales/farmacología , Esporas/efectos de los fármacos , Tricotecenos/farmacología , Verduras/química , Estructura Molecular , Phytophthora/citología , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Esporas/citología , Tricotecenos/química , Tricotecenos/aislamiento & purificaciónRESUMEN
Trichodiene is the first and only volatile intermediate in the biosynthesis of Fusarium mycotoxins and its detection in the gas-phase might therefore be of potential interest as a marker for food safety analysis. We herein present an improved diastereoselective synthesis of trichodiene which can be used as an analytical standard for a headspace gas chromatography / mass spectrometry method to be developed.
Asunto(s)
Ciclohexenos/química , Ciclohexenos/síntesis química , Micotoxinas/química , Sesquiterpenos/química , Sesquiterpenos/síntesis química , Tricotecenos/química , Estructura MolecularRESUMEN
Propolis cinnamic acid derivatives have a number of biological activities including anti-oxidant and anti-cancer ones. In this study, we aimed to elucidate the mechanism of the anti-cancer activity of 3 representative propolis cinnamic acid derivatives, i.e., Artepilin C, Baccharin and Drupanin in human colon cancer cell lines. Our study demonstrated that these compounds had a potent apoptosis-inductive effect even on drug-resistant colon cancer cells. Combination treatment of human colon cancer DLD-1 cells with 2 of these compounds, each at its IC20 concentration, induced apoptosis by stimulating both intrinsic and extrinsic apoptosis signaling pathways. Especially, Baccharin plus Drupanin exhibited a synergistic growth-inhibitory effect by strengthening both intrinsic and extrinsic apoptotic signaling transduction through TRAIL/DR4/5 and/or FasL/Fas death-signaling loops and by increasing the expression level of miR-143, resulting in decreased expression levels of the target gene MAPK/Erk5 and its downstream target c-Myc. These data suggest that the supplemental intake of these compounds found in propolis has enormous significance with respect to cancer prevention.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Cinamatos/farmacología , Neoplasias del Colon/tratamiento farmacológico , Regulación Neoplásica de la Expresión Génica , MicroARNs/genética , Própolis/farmacología , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Apoptosis/efectos de los fármacos , Inhibidores de Caspasas/farmacología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cinamatos/química , Cinamatos/aislamiento & purificación , Sinergismo Farmacológico , Proteína Ligando Fas/genética , Femenino , Fluorouracilo/farmacología , Humanos , Medicina Tradicional , Modelos Biológicos , Compuestos Organoplatinos/farmacología , Oxaliplatino , Fenilpropionatos/química , Fenilpropionatos/aislamiento & purificación , Fenilpropionatos/farmacología , Própolis/análogos & derivados , Própolis/química , Própolis/aislamiento & purificación , Transducción de Señal/efectos de los fármacos , Ligando Inductor de Apoptosis Relacionado con TNF/genética , Tricotecenos/química , Tricotecenos/aislamiento & purificación , Tricotecenos/farmacología , Regulación hacia ArribaRESUMEN
A reliable and sensitive method for rapid simultaneous determination of two type A (T-2 and diacetoxyscirpenol) and two type B (3-acetyldeoxynivalenol and Fusarenon X) trichothecenes was developed and successfully applied for detecting trichothecenes in potato tubers by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry. The established method was further evaluated by determining the linearity (R ≥ 0.9995), recovery (113.28-77.97%), precision (relative standard deviation ≤ 5.89), and sensitivity (limit of detection, 0.002-0.005 µg/g; limit of quantitation, 0.005-0.015 µg/g). The method proved to be suitable for simultaneous determination of T-2, diacetoxyscirpenol, 3-acetyldeoxynivalenol, and Fusarenon X in potato tubers inoculated with Fusarium sulphureum . In addition, it was found that T-2, diacetoxyscirpenol, 3-acetyldeoxynivalenol, and Fusarenon X could be predominantly detected in the lesion, and the toxin could also be identified in tubers without any disease symptoms. The experimental results also indicated that the concentration of toxin in the susceptible cultivar (Longshu No. 3) was significantly higher than that in the resistant cultivar (Longshu No. 6).
Asunto(s)
Contaminación de Alimentos , Inspección de Alimentos/métodos , Fusarium/metabolismo , Tubérculos de la Planta/química , Solanum tuberosum/química , Tricotecenos/análisis , Cromatografía Líquida de Alta Presión , Límite de Detección , Inmunidad de la Planta , Tubérculos de la Planta/inmunología , Tubérculos de la Planta/microbiología , Solanum tuberosum/inmunología , Solanum tuberosum/microbiología , Espectrometría de Masa por Ionización de Electrospray , Toxina T-2/análisis , Toxina T-2/química , Toxina T-2/metabolismo , Espectrometría de Masas en Tándem , Tricotecenos/química , Tricotecenos/metabolismoRESUMEN
Brazilian green propolis is a popular health supplement because of its various biological properties. The ethanol extract of Brazilian green propolis (EEBP) is characteristic for its herb-like smell and unique pungent taste. However, the ingredients responsible for its pungency have not yet been identified. This study provides the first evidence that artepillin C is the main pungent ingredient in EEBP and that it potently activates human transient receptor potential ankyrin 1 (TRPA1) channels. EEBP was fractionated using column chromatography with a step gradient elution of an ethanol-water solution, and the fractions having the pungent taste were determined by sensory tests. HPLC analysis revealed that the pungent fraction was composed primarily of artepillin C, a prenylated derivative of cinnamic acid. Artepillin C was also identified as the pungent compound of EEBP by organoleptic examiners. Furthermore, the effects of artepillin C and other cinnamic acids found in EEBP on TRPA1 channels were examined by calcium imaging and plate reader-based assays in human TRPA1-expressing cells to investigate the molecular mechanisms underlying their pungent tastes. Artepillin C and baccharin activated the TRPA1 channel strongly, whereas drupanin caused a slight activation and p-coumaric acid showed no activation. Because the EC(50) values of artepillin C, baccharin, and allyl isothiocyanate were 1.8 µM, 15.5 µM, and 6.2 µM, respectively, artepillin C was more potent than the typical TRPA1 agonist allyl isothiocyanate. These findings strongly indicate that artepillin C is the main pungent ingredient in EEBP and stimulates a pungent taste by activating TRPA1 channels.
Asunto(s)
Canales de Calcio/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Fenilpropionatos/análisis , Gusto , Canales de Potencial de Receptor Transitorio/metabolismo , Calcio/metabolismo , Línea Celular , Química Farmacéutica/métodos , Cromatografía Líquida de Alta Presión/métodos , Cinamatos/farmacología , Ácidos Cumáricos/análisis , Suplementos Dietéticos , Relación Dosis-Respuesta a Droga , Diseño de Fármacos , Humanos , Isotiocianatos/química , Modelos Químicos , Fenilpropionatos/química , Extractos Vegetales/farmacología , Propionatos , Própolis/metabolismo , Canal Catiónico TRPA1 , Transfección , Tricotecenos/químicaRESUMEN
The current experiment was conducted to determine the effect of mycotoxin-contaminated diets with aflatoxin (AFLA) and deoxynivalenol (DON) and dietary inclusion of deactivation compound on layer hen performance during a 10-wk trial. The experimental design consisted of a 4 × 2 factorial with 4 toxin levels: control, low (0.5 mg/kg AFLA + 1.0 mg/kg DON), medium (1.5 mg/kg AFLA + 1.5 mg/kg DON), and high (2.0 mg/kg AFLA + 2.0 mg/kg DON) with or without the inclusion of deactivation compound. Three hundred eighty-four 25-wk-old laying hens were randomly assigned to 1 of the 8 treatment groups. Birds were fed contaminated diets for a 6-wk phase of toxin administration followed by a 4-wk recovery phase, when all birds were fed mycotoxin-free diets. Twelve hens from each treatment were subjected to necropsy following each phase. Relative liver and kidney weights were increased (P < 0.05) at the medium and high toxin levels following the toxin phase, but the deactivation compound reduced (P < 0.05) relative liver and kidney weights following the recovery period. The high toxin level decreased (P < 0.05) feed consumption and egg production during the toxin period, whereas the deactivation compound increased (P < 0.05) egg production during the first 2 wk of the toxin phase. Egg weights were reduced (P < 0.05) in hens fed medium and high levels of toxin. An interaction existed between toxin level and deactivation compound inclusion with regard to feed conversion (g of feed/g of egg). High inclusion level of toxins increased feed conversion compared with the control diet, whereas deactivation compound inclusion reduced feed conversion to a level comparable with the control. These data indicate that deactivation compound can reduce or eliminate adverse effects of mycotoxicoses in peak-performing laying hens.
Asunto(s)
Aflatoxinas/toxicidad , Alimentación Animal/análisis , Pollos/fisiología , Dieta/veterinaria , Contaminación de Alimentos , Tricotecenos/toxicidad , Aflatoxinas/antagonistas & inhibidores , Aflatoxinas/química , Animales , Suplementos Dietéticos , Esquema de Medicación , Femenino , Riñón/anatomía & histología , Riñón/efectos de los fármacos , Hígado/anatomía & histología , Hígado/efectos de los fármacos , Bazo/anatomía & histología , Bazo/efectos de los fármacos , Tricotecenos/antagonistas & inhibidores , Tricotecenos/químicaRESUMEN
An experiment was conducted to determine the effect of dietary inclusion of Mycofix Select (Biomin GmbH, Herzogenburg, Austria) on discrete egg parameters and quality characteristics of hens fed mycotoxin-contaminated diets (aflatoxin; AFLA) and deoxynivalenol (DON)) during a 10-wk trial. A 4 × 2 factorial design was used with 4 contamination levels: control, low (0.5 mg/kg of AFLA + 1.0 mg/kg of DON), medium (1.5 mg/kg of AFLA + 1.5 mg/kg of DON), and high (2.0 mg/kg of AFLA + 2.0 mg/kg of DON) with or without the inclusion of mycotoxin deactivating compound. Three hundred and eighty-four 25-wk-old laying hens were housed 3 per cage. Birds were fed contaminated diets for a 6-wk phase of toxin administration followed by a 4-wk recovery phase, when all birds were fed mycotoxin-free diets. Parameters evaluated included egg weight, Haugh unit value, specific gravity, eggshell thickness, egg shape index, and relative albumen and yolk weights. Albumen height and Haugh unit value were depressed (P < 0.05) at the high mycotoxin level 2 wk postinclusion. Egg weight was significantly reduced (P < 0.05) with the high toxins level by the third week of toxin administration and remained throughout the study during toxin administration. Egg shape index indicated a variation (P < 0.05) in shape with all toxin levels compared with the control. Relative yolk weight was decreased (P < 0.05) by the high toxin level. An interaction existed between the deactivating compound inclusion and toxins level with regard to specific gravity. Following the toxin phase, the deactivating compound inclusion increased (P < 0.05) egg specific gravity in the control and low toxin groups whereas a decrease (P < 0.05) was observed at the high toxin level. These data indicate that mycotoxins present in feed can reduce egg quality, size, yolk weight, and alter egg shape and that inclusion of a mycotoxin deactivating compound can ameliorate some of the negative effects of mycotoxin consumption.
Asunto(s)
Aflatoxinas/toxicidad , Alimentación Animal/análisis , Pollos/fisiología , Dieta/veterinaria , Contaminación de Alimentos , Tricotecenos/toxicidad , Aflatoxinas/antagonistas & inhibidores , Aflatoxinas/química , Animales , Suplementos Dietéticos , Esquema de Medicación , Cáscara de Huevo/efectos de los fármacos , Femenino , Tricotecenos/antagonistas & inhibidores , Tricotecenos/químicaRESUMEN
It is recognized that mycotoxins can cause a variety of adverse health effects in animals, including altered gastrointestinal barrier function. It is the aim of the present study to determine whether mycotoxin-contaminated diets can alter the oral bioavailability of the antibiotics doxycycline and paromomycin in pigs, and whether a mycotoxin adsorbing agent included into diets interacts with those antibiotics. Experiments were conducted with pigs utilizing diets that contained blank feed, mycotoxin-contaminated feed (T-2 toxin or deoxynivalenol), mycotoxin-contaminated feed supplemented with a glucomannan mycotoxin binder, or blank feed supplemented with mycotoxin binder. Diets with T-2 toxin and binder or deoxynivalenol and binder induced increased plasma concentrations of doxycycline administered as single bolus in pigs compared to diets containing blank feed. These results suggest that complex interactions may occur between mycotoxins, mycotoxin binders, and antibiotics which could alter antibiotic bioavailability. This could have consequences for animal toxicity, withdrawal time for oral antibiotics, or public health.
Asunto(s)
Antibacterianos/farmacocinética , Doxiciclina/farmacocinética , Mananos/administración & dosificación , Paromomicina/farmacocinética , Toxina T-2/administración & dosificación , Tricotecenos/administración & dosificación , Adsorción , Alimentación Animal , Animales , Antibacterianos/sangre , Disponibilidad Biológica , Dieta , Doxiciclina/sangre , Mananos/química , Paromomicina/sangre , Porcinos , Toxina T-2/química , Tricotecenos/químicaRESUMEN
Unground wheat kernels contaminated with 2.09 mg deoxynivalenol (DON) per kg dry matter were stored for up to 56 days at moisture contents of 15, 17.5 and 20% to study the alterations of DON concentration when the wheat was stored either unsupplemented or supplemented with 5 g sodium metabisulfite (Na2S2O5, SBS), 10 g propionic acid (PA) or 5 g SBS plus 10 g PA per kg. SBS addition alone or in combination with PA reduced the DON contamination to 1.2-4.3% of the initial DON concentration while DON concentration of unsupplemented and wheat batches supplemented only with PA varied inconsistently or remained unchanged. The SBS-related DON reduction was paralleled by a concomitant increase in the concentration of the non-toxic reaction product DON sulfonate. In contrast to the unsupplemented wet-stored controls, SBS addition prevented the growth of moulds and yeasts when added alone or in combination with PA. In conclusion, for the conditions examined, the wet preservation of DON-contaminated wheat with SBS seems to be promising as an on-farm detoxification measure.