Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
1.
EBioMedicine ; 53: 102677, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32114389

RESUMO

BACKGROUND: Endothelial cells (ECs) function as an instructive platform to support haematopoietic stem cell (HSC) homeostasis. Our recent studies found that impaired bone marrow (BM) ECs are responsible for the defective haematopoiesis in patients with poor graft function (PGF), which is characterised by pancytopenia post-allotransplant. Although activated autophagy was reported to benefit ECs, whether EC autophagy plays a critical role in supporting HSCs and its effect on PGF patients post-allotransplant remain unclear. METHODS: To evaluate whether the autophagy status of ECs modulates their ability to support haematopoiesis, human umbilical vein endothelial cells (HUVECs) and primary BM ECs derived from healthy donors were subjected to knockdown or overexpression of Beclin-1 (an autophagy-related protein). Moreover, BM ECs derived from PGF patients were studied. FINDINGS: Beclin-1 knockdown significantly reduced the haematopoiesis-supporting ability of ECs by suppressing autophagy, which could be restored by activating autophagy via Beclin-1 upregulation. Moreover, autophagy positively regulated haematopoiesis-related genes in HUVECs. Subsequently, a prospective case-control study demonstrated that defective autophagy reduced Beclin-1 expression and the colony-forming unit (CFU) plating efficiency in BM ECs from PGF patients compared to matched patients with good graft function. Rapamycin, an autophagy activator, quantitatively and functionally improved BM ECs from PGF patients in vitro and enhanced their ability to support HSCs by activating the Beclin-1 pathway. INTERPRETATION: Our results suggest that the autophagy status of ECs modulates their ability to support haematopoiesis by regulating the Beclin-1 pathway. Defective autophagy in BM ECs may be involved in the pathogenesis of PGF post-allotransplant. Rapamycin provides a promising therapeutic approach for PGF patients. FUNDING: Please see funding sources.


Assuntos
Autofagia , Endotélio Vascular/metabolismo , Hematopoese , Pancitopenia/metabolismo , Proteína Beclina-1/genética , Proteína Beclina-1/metabolismo , Transfusão de Sangue Autóloga/efeitos adversos , Células Cultivadas , Endotélio Vascular/citologia , Células-Tronco Hematopoéticas/citologia , Células-Tronco Hematopoéticas/metabolismo , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Monócitos/citologia , Monócitos/metabolismo , Pancitopenia/etiologia
2.
Microb Ecol ; 80(1): 1-13, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31838570

RESUMO

Antimicrobial resistance (AMR) in the aquatic environment has received increasing attention in recent years, and growing eutrophication problems may contribute to AMR in aquatic ecosystems. To evaluate whether and how eutrophication affects AMR, 40 surface water samples were collected from the Minjiang River, Fujian Province, China. Total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (CODMn) were measured as eutrophication factors. Additionally, enterococci species were isolated and their resistance to six common antibiotics was tested. Eutrophication generally showed a trend of increasing with the flow direction of the Minjiang River, with 25 sites (62.5%) having a TN/TP value over the Redfield value (16:1), which indicated that eutrophication in this region was of phosphorus limitation. High nutrition sites were in or near urban areas. Poor quality water was found in the middle and lower reaches of the Minjiang River system. The resistance frequency of 40 enterococci isolates to the six antibiotics tested was as follows: oxytetracycline > erythromycin > ciprofloxacin > chloramphenicol > ampicillin > vancomycin (70, 50, 17.5, 12.5, 2.5, 0%), and the multi-resistant rate reached 50% with eight resistance phenotypes. AMR also increased along the direction of water flow downstream, and most of the sites with the highest AMR were in or near urban areas, as was true for nutrition levels. Positive correlations between AMR and eutrophication factors (TN, TP, and CODMn) were identified using the Pearson's correlation coefficient, and TN/TP generally was negatively related to AMR. These results indicated that eutrophication may induce or selective for resistance of water-borne pathogens to antibiotics, with a high resistance level and a wide resistance spectrum.


Assuntos
Resistência Microbiana a Medicamentos , Enterococcus/efeitos dos fármacos , Eutrofização , Rios/microbiologia , Análise da Demanda Biológica de Oxigênio , China , Enterococcus/isolamento & purificação , Enterococcus/fisiologia , Nitrogênio/análise , Fósforo/análise , Rios/química
3.
Eur J Haematol ; 96(3): 297-308, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26010204

RESUMO

This study investigated the prognostic factors and clinical outcomes of preemptive chemotherapy followed by granulocyte colony-stimulating factor-primed donor leukocyte infusion (Chemo-DLI) according to minimal residual disease (MRD) status in patients with acute leukemia and myelodysplastic syndromes who received allogeneic hematopoietic stem cell transplantation (HSCT) (n = 101). Patients received immunosuppressive drugs to prevent graft-vs.-host disease (GVHD) after Chemo-DLI. The 3-yr cumulative incidences of relapse, non-relapse mortality, and disease-free survival (DFS) after HSCT were 39.5%, 9.6%, and 51.7%, respectively. The cumulative incidences of relapse and DFS were significantly poorer in patients who exhibited early-onset MRD. Forty-four patients turned MRD negative 1 month after Chemo-DLI; their cumulative incidences of relapse and DFS were significantly better than those with persistent MRD 1 month after preemptive Chemo-DLI (relapse: 19.8% vs. 46.8%, P = 0.001; DFS: 69.6% vs. 46.4%, P = 0.004). The cumulative incidences of relapse and DFS after HSCT were significantly better in patients with chronic GVHD (cGVHD) than those without cGVHD (relapse: 19.6% vs. 63.7%, P < 0.001; DFS: 74.4% vs. 23.8%, P < 0.001). Early-onset MRD, persistent MRD after Chemo-DLI, and non-cGVHD after Chemo-DLI, which were associated with increased relapse and impaired DFS, suggest unsatisfactory response to preemptive Chemo-DLI.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Doença Enxerto-Hospedeiro/prevenção & controle , Leucemia/terapia , Transfusão de Leucócitos , Síndromes Mielodisplásicas/terapia , Doença Aguda , Adolescente , Adulto , Transfusão de Sangue Autóloga , Criança , Pré-Escolar , Feminino , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Antígenos HLA/genética , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Humanos , Leucemia/diagnóstico , Leucemia/mortalidade , Masculino , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/diagnóstico , Síndromes Mielodisplásicas/mortalidade , Neoplasia Residual/diagnóstico , Prognóstico , Recidiva , Terapia de Salvação , Análise de Sobrevida , Condicionamento Pré-Transplante , Transplante Homólogo , Resultado do Tratamento , Adulto Jovem
4.
Guang Pu Xue Yu Guang Pu Fen Xi ; 31(3): 820-2, 2011 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-21595247

RESUMO

The method for the determination of 5 kinds of trace heavy metal elements (Cu, As, Hg, Cd and Pb) in Fructus Aurantii by inductively coupled plasma-mass (ICP-MS) with HNO3-H2O2 microwave digestion was established. The recoveries of the elements detected were in the range of 85%-109% and the relative standard deviations (RSD) was in the range of 3.6%-5.4%. It indicates that the method is rapid, sensitive and accurate. It was suitable for the determination of the contents of 5 trace heavy metal elements in Fructus Aurantii. The dissolution characteristics of the 5 heavy metal elements in different extraction methods (microwave-assisted extraction, ultrasonic extraction and decocting extraction, respectively) were studied. The results showed that the concentrations of trace elements As and Pb obtained by microwave-assisted extraction were relatively lower than that by ultrasonic extraction and decocting extraction. The dissolving concentrations of the 5 trace heavy metal elements (Cu, As, Hg, Cd and Pb) in Fructus Aurantii in different extracting methods were all lower than the limits of Chinese Pharmacopoeia and Green Trade Standard for Importing and Exporting Medicinal Plant and Preparation. Microwave-assisted extraction for effective constituent was rapid, effective and safe.


Assuntos
Espectrometria de Massas/métodos , Metais Pesados/análise , Rutaceae/química
5.
J Exp Bot ; 61(6): 1827-38, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20231328

RESUMO

Gamma-linolenic acid (gamma-linolenic acid, GLA; C18:3 Delta(6, 9, 12)) belongs to the omega-6 family and exists primarily in several plant oils, such as evening primrose oil, blackcurrant oil, and borage oil. Delta(6)-desaturase is a key enzyme involved in the synthesis of GLA. There have been no previous reports on the genes encoding Delta(6)-desaturase in blackcurrant (Ribes nigrum L.). In this research, five nearly identical copies of Delta(6)-desaturase gene-like sequences, named RnD8A, RnD8B, RnD6C, RnD6D, and RnD6E, were isolated from blackcurrant. Heterologous expression in Saccharomyces cerevisiae and/or Arabidopsis thaliana confirmed that RnD6C/D/E were Delta(6)-desaturases that could use both alpha-linolenic acids (ALA; C18:3 Delta(9,12,15)) and linoleic acid (LA; C18:2 Delta(9,12)) precursors in vivo, whereas RnD8A/B were Delta(8)-sphingolipid desaturases. Expression of GFP tagged with RnD6C/D/E showed that blackcurrant Delta(6)-desaturases were located in the mitochondrion (MIT) in yeast and the endoplasmic reticulum (ER) in tobacco. GC-MS results showed that blackcurrant accumulated GLA and octadecatetraenoic acids (OTA; C18:4 Delta(6,9,12,15)) mainly in seeds and a little in other organs and tissues. RT-PCR results showed that RnD6C and RnD6E were expressed in all the tissues at a low level, whereas RnD6D was expressed at a high level only in seeds, leading to the accumulation of GLA and OTA in seeds. This research provides new insights to our understanding of GLA synthesis and accumulation in plants and the evolutionary relationship of this class of desaturases, and new clues as to the amino acid determinants which define precise enzyme activity.


Assuntos
Ácidos Graxos Dessaturases/metabolismo , Plantas Geneticamente Modificadas/enzimologia , Ribes/enzimologia , Sequência de Aminoácidos , Ácidos Graxos Dessaturases/química , Ácidos Graxos Dessaturases/genética , Cromatografia Gasosa-Espectrometria de Massas , Dados de Sequência Molecular , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Ribes/genética , Ribes/metabolismo , Homologia de Sequência de Aminoácidos , Nicotiana/enzimologia , Nicotiana/genética , Nicotiana/metabolismo
6.
Guang Pu Xue Yu Guang Pu Fen Xi ; 28(9): 2173-6, 2008 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-19093587

RESUMO

The Se in the blood and tissue of animal samples was determined by ICP-MS instrument. The method was not only proved simple and rapid, but also showed satisfying precision and low detection limit. The optimum conditions of ICP-MS for the method were investigated. Results by the digestion method, the isotopes selection, and the difference between the normal ICP-MS without ORS (Octopole Reaction System) and the ICP-MS with ORS were compared, which afford reference to the determination of the same kinds of sample. The standard materials were determined to proved the reliability of the normal ICP-MS and the ORS-ICP-MS methods. The ORS-ICP-MS can effectively remove the interference based on polyatomic ions, so the the detection limits were lowed, and the accuracy was improved. The detection limits of the method for normal ICP-MS is 0.024 ng x g(-1) and that for ORS-ICP-MS is 0.004 6 ng x g(-1), the RSDs are between 1.8% and 5.5%, and the recoveries of the method are in the range of 90.8%-107.2%.


Assuntos
Selênio/sangue , Selênio/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Animais , Cobaias , Metais Pesados/sangue , Metais Pesados/metabolismo , Reprodutibilidade dos Testes
7.
J Clin Immunol ; 28(3): 276-83, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18204965

RESUMO

We evaluated the safety and efficacy of donor lymphocyte infusion (DLI) with granulocyte colony-stimulating factor priming and short-term immunosuppressive agents for prophylaxis of relapse in patients with advanced leukemia after human leukocyte antigen (HLA)-mismatched T cell-replete hematopoietic stem cell transplantation (HCT). Twenty-nine patients received prophylactic DLI at a median 75 (33-120) days after HCT. Acute graft-vs-host disease (GVHD) grades 3-4 occurred in six patients, and all cases were controlled. Eleven patients were alive and relapse-free with a probability of leukemia-free survival (LFS) of 37.3 +/- 9.6% at 3 years. Chronic GVHD was associated with a lower relapse rate and higher probability of LFS. Prophylactic-modified DLI is feasible in patients with advanced leukemia to prevent relapse after HLA-mismatched HCT.


Assuntos
Antígenos HLA/genética , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade , Leucemia Mieloide Aguda/prevenção & controle , Transfusão de Linfócitos , Recidiva Local de Neoplasia/prevenção & controle , Linfócitos T/transplante , Adolescente , Adulto , Transfusão de Sangue Autóloga , Intervalo Livre de Doença , Feminino , Doença Enxerto-Hospedeiro , Humanos , Leucemia Mieloide Aguda/imunologia , Depleção Linfocítica , Masculino , Pessoa de Meia-Idade , Linfócitos T/imunologia
8.
Zhong Yao Cai ; 31(10): 1562-4, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19230417

RESUMO

OBJECTIVE: To study on ursolic acid extraction rate from leaves of Paulownia fortunei by conventional powder and ultramicro powder. METHODS: We spent different time extracting ursolic acid from coventional powder and ultramicro powder, then determined the content by HPLC and compared. RESULTS: In ultramicro powder the extraction of ursolic acid was nearly twice of conventional powder; The time reaching the maximum extraction of ultramicro powder was 5 minutes, but that of conventional powder was 20 minutes. CONCLUSIONS: The extraction of ursolic acid from leaves of Paulownia fortunei by ultramicro smashing is more efficient.


Assuntos
Plantas Medicinais/química , Scrophulariaceae/química , Tecnologia Farmacêutica/métodos , Triterpenos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Tamanho da Partícula , Folhas de Planta/química , Pós , Reprodutibilidade dos Testes , Fatores de Tempo , Triterpenos/análise , Ácido Ursólico
9.
Guang Pu Xue Yu Guang Pu Fen Xi ; 26(12): 2326-9, 2006 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-17361742

RESUMO

A method was studied for the analysis of 11 kinds of trace elements(Cr, Mn, Ni, Co, Cu, Zn, As, Se, Mo, Cd and Pb) in root-like and rootstalk-like dried medicinal herbs by microwave digestion-ICP-MS. A digestion method was developed for trace elements analysis for two types of herbs. The Microwave digestion condition was optimized in terms of digestion system, proportion of acids, and digestion procedure, which affords reference to the preparation of the same kinds of dried medicinal herbs. The detection limits of the method for all sample elements were in the range of 0. 001-0. 260 microg x g(-1), the RSDs were between 0. 4%-3. 1% and the recoveries were between 90% and 110%.


Assuntos
Medicamentos de Ervas Chinesas/química , Espectrometria de Massas , Micro-Ondas , Oligoelementos/análise
10.
Beijing Da Xue Xue Bao Yi Xue Ban ; 37(3): 231-5, 2005 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-15968308

RESUMO

OBJECTIVE: To explore the optimal time of allogeneic hematopoietic stem cell transplantation (allo-HSCT) applied in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL), and the optimum order of donor selection. METHODS: From Mar 2000 to Jul 2004, 32 patients with Ph+ ALL were treated by Allo-HSCT, and the follow up ended at Dec 30.2004 with medium of 13 months. Of whom, 24 were male, and 8 were female. Twenty-three patients have been transplanted in CR1, 9 patients beyond CR1 (1 in CR2, 8 in refractory or relapse status). Twelve cases received HSCT from identical sibling donor, 4 unrelated Cord Blood Transplantation (CBT), 3 HSCT from matched unrelated donor (MUD), and 13 HSCT from mismatched related donor (MMRD), of which, 6 cases were M(BCR/ABL) subtype, and 20 m(BCR/ABL) subtype. The Kaplan-Meier method was used to estimate the probabilities of leukemia-free survival (LFS), overall survival (OS) and relapse incidence (RI), and the factors were compared by means of the Log-rank test. Simultaneous effect of multiple covariates were estimated with Cox model. RESULTS: Of all the 32 cases engrafted, 4-year OS was 57.19%, LFS 37.09%, and RI 56.36%. In univariate prognostic analysis model, the OS was higher in CR1 group pre-HSCT than that in non-CR1 group (74.50% vs 22.22%, P=0.0046), LFS was higher (49.06% vs 11.11%, P=0.0057), RI was lower (44.80% vs 84.76%, P=0.0157); the OS was higher in M(BCR/ABL) group than that in m(BCR/ABL) group (100% vs 40.91%, P=0.0318), LFS was higher (75% vs 17.72%, P=0.0057), RI was lower (25% vs 77.88, P=0.0116); OS was similar in HLA MM RD group to that in HLA identical group (52.65% vs 55.56%, P=0.6247), LFS was similar (45.12% vs 30.00%, P=0.8315), and RI was also similar (50.77% vs 60.62%, P=0.8217). In multiple covariate analysis model, the BCR/ABL type was the risk factor of LFS [P=0.005, Exp(B)=9.971] and RI (P=0.006, Exp(B)=9.488), the status of disease pre-HSCT and BCR/ABL subtype was the risk factor of OS [P was 0.010 and 0.038, Exp(B) was 4.532 and 37.537 respectively]. CONCLUSION: We had better do HSCT in CR1 to treat Ph+ ALL, if patient is refractory to chemotherapy, we can try STI571, and HSCT should be done as soon as possible if the patients get CR. Mismatched related donor is considered as regular donor for Ph+ ALL patients without identical donors. We should pay more attention to monitoring and prophylaxis of relapse in m(BCR/ABL) patients after HSCT.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Adolescente , Adulto , Criança , Feminino , Seguimentos , Proteínas de Fusão bcr-abl/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Transplante Homólogo
11.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 24(3): 213-5, 2004 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15074087

RESUMO

OBJECTIVE: To evaluate the effect of Xuesaitong injection (XSTI, a preparation of Panax Notoginseng) as auxilliary treatment of severe craniocerebral injury. METHODS: Eighty-seven patients with severe craniocerebral injury were selected and randomly divided into the treated group (n = 44) and the control group (n = 43), they were treated with conventional treatment, and XSTI was given additionally to the treated group. Intracranial pressure (ICP) and Glasgow coma score (GCS) of all patients were measured after 1 or 2-week treatment, and Glasgow outcome scale (GOS) of them was determined 3 months later. Then the therapeutic effect in the two groups were compared. RESULTS: After treatment, the ICP was lower, GCS higher and GOS better in the treated group than those in the control group significantly, all showing statistical significance (P < 0.05). CONCLUSION: XSTI has marked clinical therapeutic effect in treating patients with severe craniocerebral injury.


Assuntos
Traumatismos Craniocerebrais/tratamento farmacológico , Panax , Fitoterapia , Adulto , Lesões Encefálicas/tratamento farmacológico , Lesões Encefálicas/cirurgia , Traumatismos Craniocerebrais/cirurgia , Quimioterapia Combinada , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Escala de Coma de Glasgow , Escala de Resultado de Glasgow , Humanos , Masculino , Fármacos Neuroprotetores/uso terapêutico , Panax/química , Saponinas/administração & dosagem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA