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1.
PLoS One ; 16(5): e0252630, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34048486

RESUMO

AIM: Recently, the level of growth differentiation factor 15 (GDF-15) in blood, was proposed as biomarker to detect mitochondrial dysfunction. In the current study, we evaluate this biomarker in open-angle glaucoma (OAG), as there is increasing evidence that mitochondrial dysfunction plays a role in the pathophysiology of this disease. METHODS: Plasma GDF-15 concentrations were measured with ELISA in 200 OAG patients and 61 age-matched controls (cataract without glaucoma). The OAG patient group consisted of high tension glaucoma (HTG; n = 162) and normal tension glaucoma (NTG; n = 38). Groups were compared using the Kruskal-Wallis nonparametric test with Dunn's multiple comparison post-hoc correction. GDF-15 concentration was corrected for confounders identified with forward linear regression models. RESULTS: Before correcting for confounders, median plasma GDF-15 levels was significantly lower in the combined OAG group (p = 0.04), but not when analysing HTG and NTG patients separately. Forward linear regression analysis showed that age, gender, smoking and systemic hypertension were significant confounders affecting GDF-15 levels. After correction for these confounders, GDF-15 levels in OAG patients were no longer significantly different from controls. Subgroup analysis of the glaucoma patients did not show a correlation between disease severity and plasma GDF-15, but did reveal that for NTG patients, intake of dietary supplements, which potentially improve mitochondrial function, correlated with lower plasma GDF-15. CONCLUSION: The present study suggests that plasma GDF-15 is not suited as biomarker of mitochondrial dysfunction in OAG patients.


Assuntos
Glaucoma de Ângulo Aberto/patologia , Fator 15 de Diferenciação de Crescimento/sangue , Idoso , Estudos de Casos e Controles , Feminino , Glaucoma de Ângulo Aberto/sangue , Humanos , Pressão Intraocular , Estilo de Vida , Modelos Lineares , Glaucoma de Baixa Tensão/sangue , Glaucoma de Baixa Tensão/patologia , Masculino , Pessoa de Meia-Idade
2.
Tissue Eng Part B Rev ; 25(5): 412-428, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31088331

RESUMO

Glaucoma is a major eye disease characterized by a progressive loss of retinal ganglion cells (RGCs). Biomechanical forces as a result of hydrostatic pressure and strain play a role in this disease. Decreasing intraocular pressure is the only available therapy so far, but is not always effective and does not prevent blindness in many cases. There is a need for drugs that protect RGCs from dying in glaucoma; to develop these, we need valid glaucoma and drug screening models. Since in vivo models are unsuitable for screening purposes, we focus on in vitro and ex vivo models in this review. Many groups have studied pressure and strain model systems to mimic glaucoma, to investigate the molecular and cellular events leading to mechanically induced RGC death. Therefore, the focus of this review is on the different mechanical model systems used to mimic the biomechanical forces in glaucoma. Most models use either cell or tissue strain, or fluid- or gas-controlled hydrostatic pressure application and apply it to the relevant cell types such as trabecular meshwork cells, optic nerve head astrocytes, and RGCs, but also to entire eyes. New model systems are warranted to study concepts and test experimental compounds for the development of new drugs to protect vision in glaucoma patients. Impact Statement The outcome of currently developed models to investigate mechanically induced retinal ganglion cell death by applying different mechanical strains varies widely. This suggests that a robust glaucoma model has not been developed yet. However, a comprehensive overview of current developments is not available. In this review, we have therefore assessed what has been done before and summarized the available knowledge in the field, which can be used to develop improved models for glaucoma research.


Assuntos
Modelos Animais de Doenças , Glaucoma/tratamento farmacológico , Fármacos Neuroprotetores/farmacologia , Células Ganglionares da Retina/efeitos dos fármacos , Animais , Avaliação Pré-Clínica de Medicamentos , Glaucoma/patologia , Humanos , Pressão Intraocular , Células Ganglionares da Retina/citologia
3.
Br J Nutr ; 115(9): 1509-20, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-26983845

RESUMO

The aim of the present study was to assess the effects of dietary supplementation with epidermal growth factor (EGF)-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets. In total, forty piglets weaned at 21-26 d of age were assigned to one of the five groups that were provided basic diet (control group) or diet supplemented with S. cerevisiae expressing either empty-vector (INVSc1(EV) group), tagged EGF (T-EGF) (INVSc1-TE(-) group), extracellular EGF (EE-EGF) (INVSc1-EE(+) group) or intracellular EGF (IE-EGF) (INVSc1-IE(+) group). All treatments were delivered as 60·00 µg/kg body weight EGF/d. On 0, 7, 14 and 21 d, eight piglets per treatment were sacrificed to analyse the morphology, activities and mRNA expressions of digestive enzymes, as well as Ig levels (IgA, IgM, IgG) in duodenal mucosa. The results showed significant improvement on 7, 14 and 21 d, with respect to average daily gain (P<0·05), mucosa morphology (villus height and crypt depth) (P<0·05), Ig levels (P<0·01), activities and mRNA expressions of digestive enzymes (creatine kinase, alkaline phosphatase, lactate dehydrogenase and sucrase) (P<0·05) and the mRNA expression of EGF-receptor (P<0·01) in NVSc1-TE(-), INVSc1-EE(+) and INVSc1-IE(+) groups compared with control and INVSc1(EV) groups. In addition, a trend was observed in which the INVSc1-IE(+) group showed an improvement in Ig levels (0·05

Assuntos
Suplementos Nutricionais , Duodeno/efeitos dos fármacos , Fator de Crescimento Epidérmico/farmacologia , Mucosa Intestinal/efeitos dos fármacos , Saccharomyces cerevisiae/metabolismo , Fosfatase Alcalina/genética , Fosfatase Alcalina/metabolismo , Animais , Creatina Quinase/genética , Creatina Quinase/metabolismo , Duodeno/crescimento & desenvolvimento , Duodeno/metabolismo , Fator de Crescimento Epidérmico/administração & dosagem , Receptores ErbB/genética , Receptores ErbB/metabolismo , Imunoglobulinas/metabolismo , Mucosa Intestinal/crescimento & desenvolvimento , Mucosa Intestinal/metabolismo , L-Lactato Desidrogenase/genética , L-Lactato Desidrogenase/metabolismo , Lactococcus lactis , RNA Mensageiro/metabolismo , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacologia , Sacarase/genética , Sacarase/metabolismo , Suínos , Desmame
4.
Proc Natl Acad Sci U S A ; 110(50): 20206-11, 2013 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-24277820

RESUMO

Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes, and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ATP-binding cassette sub-family C member 6 (ABCC6), an ATP-dependent efflux transporter present mainly in the liver. Abcc6(-/-) mice have been instrumental in demonstrating that PXE is a metabolic disease caused by the absence of an unknown factor in the circulation, the presence of which depends on ABCC6 in the liver. Why absence of this factor results in PXE has remained a mystery. Here we report that medium from HEK293 cells overexpressing either human or rat ABCC6 potently inhibits mineralization in vitro, whereas medium from HEK293 control cells does not. Untargeted metabolomics revealed that cells expressing ABCC6 excrete large amounts of nucleoside triphosphates, even though ABCC6 itself does not transport nucleoside triphosphates. Extracellularly, ectonucleotidases hydrolyze the excreted nucleoside triphosphates to nucleoside monophosphates and inorganic pyrophosphate (PPi), a strong inhibitor of mineralization that plays a pivotal role in several mineralization disorders similar to PXE. The in vivo relevance of our data are demonstrated in Abcc6(-/-) mice, which had plasma PPi levels <40% of those found in WT mice. This study provides insight into how ABCC6 affects PXE. Our data indicate that the factor that normally prevents PXE is PPi, which is provided to the circulation in the form of nucleoside triphosphates via an as-yet unidentified but ABCC6-dependent mechanism.


Assuntos
Difosfatos/sangue , Doenças Metabólicas/genética , Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética , Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo , Pseudoxantoma Elástico/genética , Animais , Primers do DNA/genética , DNA Complementar/genética , Fosfatos de Dinucleosídeos/metabolismo , Células HEK293 , Humanos , Doenças Metabólicas/metabolismo , Doenças Metabólicas/patologia , Metabolômica , Camundongos , Camundongos Knockout , Mutagênese Sítio-Dirigida , Mutação/genética , Pseudoxantoma Elástico/metabolismo , Pseudoxantoma Elástico/patologia , Ratos
5.
J Mol Med (Berl) ; 89(11): 1125-35, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21725681

RESUMO

Pseudoxanthoma elasticum (PXE) is an autosomal recessive disorder in which calcification of connective tissue leads to pathology in skin, eye and blood vessels. PXE is caused by mutations in ABCC6. High expression of this transporter in the basolateral hepatocyte membrane suggests that it secretes an as-yet elusive factor into the circulation which prevents ectopic calcification. Utilizing our Abcc6 (-/-) mouse model for PXE, we tested the hypothesis that this factor is vitamin K (precursor) (Borst et al. 2008, Cell Cycle). For 3 months, Abcc6 (-/-) and wild-type mice were put on diets containing either the minimum dose of vitamin K required for normal blood coagulation or a dose that was 100 times higher. Vitamin K was supplied as menaquinone-7 (MK-7). Ectopic calcification was monitored in vivo by monthly micro-CT scans of the snout, as the PXE mouse model develops a characteristic connective tissue mineralization at the base of the whiskers. In addition, calcification of kidney arteries was measured by histology. Results show that supplemental MK-7 had no effect on ectopic calcification in Abcc6 ( -/- ) mice. MK-7 supplementation increased vitamin K levels (in skin, heart and brain) in wild-type and in Abcc6 (-/-) mice. Vitamin K tissue levels did not depend on Abcc6 genotype. In conclusion, dietary MK-7 supplementation increased vitamin K tissue levels in the PXE mouse model but failed to counteract ectopic calcification. Hence, we obtained no support for the hypothesis that Abcc6 transports vitamin K and that PXE can be cured by increasing tissue levels of vitamin K.


Assuntos
Calcinose/metabolismo , Pseudoxantoma Elástico/metabolismo , Vitamina K 2/análogos & derivados , Vitaminas/farmacologia , Vitaminas/farmacocinética , Animais , Calcinose/tratamento farmacológico , Calcinose/genética , Calcinose/patologia , Modelos Animais de Doenças , Hemostáticos/farmacocinética , Hemostáticos/farmacologia , Humanos , Camundongos , Camundongos Knockout , Pseudoxantoma Elástico/tratamento farmacológico , Pseudoxantoma Elástico/genética , Pseudoxantoma Elástico/patologia , Vitamina K 2/farmacocinética , Vitamina K 2/farmacologia
6.
J Mol Med (Berl) ; 88(5): 467-75, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20177653

RESUMO

Pseudoxanthoma elasticum (PXE) is a heritable disorder characterized by ectopic calcification of connective tissue in skin, Bruch's membrane of the eye, and walls of blood vessels. PXE is caused by mutations in the ABCC6 gene, but the exact etiology is still unknown. While observations on patients suggest that high calcium intake worsens the clinical symptoms, the patient organization PXE International has published the dietary advice to increase calcium intake in combination with increased magnesium intake. To obtain more data on this controversial issue, we examined the effect of dietary calcium and magnesium in the Abcc6(-/-) mouse, a PXE mouse model which mimics the clinical features of PXE. Abcc6(-/-) mice were placed on specific diets for 3, 7, and 12 months. Disease severity was measured by quantifying calcification of blood vessels in the kidney. Raising the calcium content in the diet from 0.5% to 2% did not change disease severity. In contrast, simultaneous increase of both calcium (from 0.5% to 2.0%) and magnesium (from 0.05% to 0.2%) slowed down the calcification significantly. Our present findings that increase in dietary magnesium reduces vascular calcification in a mouse model for PXE should stimulate further studies to establish a dietary intervention for PXE.


Assuntos
Vasos Sanguíneos/patologia , Calcinose/dietoterapia , Cálcio/metabolismo , Magnésio/metabolismo , Pseudoxantoma Elástico/dietoterapia , Transportadores de Cassetes de Ligação de ATP/genética , Animais , Calcinose/metabolismo , Calcinose/patologia , Suplementos Nutricionais , Deleção de Genes , Rim/irrigação sanguínea , Rim/patologia , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Associadas à Resistência a Múltiplos Medicamentos , Miocárdio/patologia , Pseudoxantoma Elástico/metabolismo , Pseudoxantoma Elástico/patologia
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