RESUMO
Using DNA comet assay we found that polysaccharides from Tussilago farfara L. reduced the intensity of polychemotherapy-induced apoptosis and DNA damage in bone marrow cells and small intestinal epithelium of C57Bl/6 mice, which attested to genoprotective properties of these polysaccharides.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica/antagonistas & inibidores , Células da Medula Óssea/efeitos dos fármacos , DNA/química , Mucosa Intestinal/efeitos dos fármacos , Polissacarídeos/farmacologia , Tussilago/química , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Protocolos de Quimioterapia Combinada Antineoplásica/toxicidade , Apoptose/efeitos dos fármacos , Células da Medula Óssea/citologia , Células da Medula Óssea/metabolismo , Cisplatino/antagonistas & inibidores , Cisplatino/toxicidade , Ensaio Cometa , DNA/metabolismo , Duodeno/citologia , Duodeno/efeitos dos fármacos , Duodeno/metabolismo , Feminino , Injeções Intraperitoneais , Mucosa Intestinal/citologia , Mucosa Intestinal/metabolismo , Irinotecano/antagonistas & inibidores , Irinotecano/toxicidade , Camundongos , Camundongos Endogâmicos C57BL , Paclitaxel/antagonistas & inibidores , Paclitaxel/toxicidade , Extratos Vegetais/química , Polissacarídeos/isolamento & purificaçãoRESUMO
Effectiveness of the granulocyte colony-stimulating factor immobilized by using electronbeam synthesis nanotechnology was investigated on the model of experimental testicular failure caused by the toxic effect on stem spermatogonia. Administration of the drug to experimental paclitaxel-treated animals increased the number of sources of the proliferative pool of spermatogenesis and its productivity. The effectiveness of immobilized granulocyte colony-stimulating factor was based on its ability to stimulate reparative regeneration of the spermatogenic tissue, which manifested in a decrease in spermatogenic layer maturity and increase in the number of microenvironment cells. Effectiveness of the immobilized form of the drug was superior to that of non-immobilized form.