Microalgal-based bioremediation of emerging contaminants: Mechanisms and challenges.

Emerging contaminants (ECs) in different ecosystems have consistently been acknowledged as a global issue due to toxicity, human health implications, and potential role in generating and disseminating antimicrobial resistance. The existing wastewater treatment system is incompetent at eliminating ECs since the effluent water contains significant concentrations of ECs, viz., antibiotics (0.03-13.0 µg L-1), paracetamol (50 µg L-1), and many others in varying concentrations. Microalgae are considered as a prospective and sustainable candidate for mitigating of ECs owing to some peculiar features. In addition, the microalgal-based processes also offer cost and energy-efficient solutions for the bioremediation of ECs than conventional treatment systems. It is pertinent that, microalgal-based processes also provides waste valorization benefits as microalgal biomass obtained after ECs treatment can be potentially applied to generate biofuels. Moreover, microalgae can effectively utilize alternative metabolic (cometabolism) routes for enhanced degradation of ECs. Additionally, the ECs removal via the microalgal biodegradation route is highly promising as it can transform the ECs into less toxic compounds. The present review comprehensively discusses different mechanisms involved in removing ECs and various factors that affect their removal. Also, the technoeconomic feasibility of microalgae than other conventional wastewater treatment methods is summarised. The review also highlighted the different molecular and genetic tools that can augment the activity and robustness of microalgae for better removal of organic contaminants. Finally, we have summarised the challenges and future research required towards microalgal-based bioremediation of emerging contaminants (ECs) as a holistic approach.

Nitrogen and phosphorus removals by the agar-immobilized Chlorella sacchrarophila with long-term preservation at room temperature.

Immobilized microalgae have great potential on the nutrient removal during wastewater treatment. However, their applications are challenged by how to cost-effectively maintain and preserve large number of viable and active microalgal cells. In this study, the cells of Chlorella sacchrarophila were immobilized in the agar containing with algal nutrient, encapsulated in a transparent package and preserved at room temperature. After the preservation for 200 days, microalgal cells with viability of 47-52% were maintained and could be quickly revived after the cultivation in fresh algal medium. Based on the agar-immobilized microalgae, the NH4+-N and PO43--P were efficiently removed from batch and continuous cultures, with the highest removal efficiencies ranging from 96% to 99% were observed. Even being recycled for eight times, the agar-immobilized microalgae were still able to remove 94% of NH4+-N and 66% of PO43--P. Moreover, more than 60% of the nutrient removal efficiency was determined even the agar-immobilized microalgae being preserved for 120 days at room temperature. This work provides a simple, cost-effective and practicable method for the long-term preservation of microalgae at room temperature, which makes the application of microalgal species on the nutrient removal during wastewater treatment more convenient and useful.

Efficient engineered probiotics using synthetic biology approaches: A review.

The uses of probiotics-based food supplements are getting emphasis due to their power to ensure better health conditions. Probiotics have diverse and significant applications in the health sector, so probiotic strains require an understanding of the genome level organizations. Probiotics elucidate various functional parameters that control their metabolic functions. In this review, we have compiled aspects of synthetic biology, which are used for the optimization of metabolic processes in probiotics for their use as a supplement in allopathic medicines. Synthetic biology approaches provide information about diverse biosynthetic pathways and also facilitate the novel metabolic engineering approaches for probiotics strain improvement. We have discussed the synthetic biology approaches for producing engineered probiotics via genetic circuits, expression systems, and genome editing tools like CRISPR-Cas and PEVLAB. This review also enlightens future challenges in the development of engineered probiotics.

Multilevel algorithms and evolutionary hybrid tools for enhanced production of arginine deiminase from Pseudomonas furukawaii RS3.

In the present study a high arginine deiminase (ADI) yielding bacterium was isolated from soil samples of Haryana, India and identified as Pseudomonas furukawaii. The specific enzyme activity was optimized to 1.420 IU/ml by OFAT and further enhanced to 2.708 IU/ml (an increase of 90.7%) with the help of statistical parametric optimization approaches using GA-ANN and GA-ANFIS. The obtained value of the coefficient of correlation (R = 0.88) for ANN and epoch error (0.12) for ANFIS, indicates the prediction accuracy and strength of these data training models. ADI production was improved significantly in simple super broth media supplemented with 1.5% fructose and 1.75% arginine at pH 7 at 37 °C using multilevel algorithms and evolutionary hybrid tools. The native enzyme was partially purified (ten-fold) up to a specific enzyme activity of 29.559 IU/mg.

Two-step statistical optimization for cold active ß-glucosidase production from Pseudomonas lutea BG8 and its application for improving saccharification of paddy straw.

ß-Glucosidase is an essential part of cellulase enzyme system for efficient and complete hydrolysis of biomass. Psychrotolerant Pseudomonas lutea BG8 produced ß-glucosidase with lower temperature optima and hence can play important role in bringing down the energy requirement for bioethanol production. To enhance ß-glucosidase production, two statistical tools: Taguchi and Box-Behnken designs were applied to reveal the most influential factors and their respective concentration for maximum production of ß-glucosidase under submerged fermentation. The optimal medium composition for maximum ß-glucosidase production were 2.99% (w/v) bagasse, 0.33% (w/v) yeast extract, 0.38% (w/v) Triton X-100, 0.39% (w/v) NaNO3 , and pH 8.0 at temperature 30 °C. Under optimized conditions, ß-glucosidase production increased up to 9.12-fold (17.52 ± 0.24 IU/g) in shake flask. Large-scale production in 7-L stirred tank bioreactor resulted in higher ß-glucosidase production (23.29 ± 0.23 IU/g) within 80 H of incubation, which was 1.34-fold higher than shake flask studies. Commercial cellulase (Celluclast® 1.5L) when supplemented with this crude ß-glucosidase resulted in improved sugar release (548.4 ± 2.76 mg/gds) from paddy straw at comparatively low temperature (40 °C) of saccharification. P. lutea BG8 therefore showed great potential for cold active ß-glucosidase production and can be used as accessory enzyme along with commercial cellulase to improve saccharification efficiency.