RESUMO
Agaricus blazei is a rare medicinal and edible fungus with a crispy taste and delicious flavor. Both fruiting body and mycelium are rich in polysaccharides, sterols, terpenoids, peptides, lipids, polyphenols, and other active ingredients, which have strong pharmacological activities such as anti-tumor, lipid-lowering, glucose-lowering, immunomodulation, optimization of intestinal flora, and anti-oxidation. Therefore, it is a kind of fungal resource with a great prospect of edible and medicinal development. Among the reported chemical components of A. blazei, blazeispirol is a series of sterol compounds unique to A. blazei, which has a spiral structure and is different from classical steroids. It is an important active ingredient found in the mycelium of A. blazei and has significant hepatoprotective activity. It can be used as a phylogenetic and chemotaxonomic marker of A. blazei strains and is considered an excellent lead compound for drug development. According to the skeleton structure characteristics, the 17 discovered blazeispirol compounds can be divided into two types: blazeispirane and problazeispirane. In order to further explore the resource of blazeispirol compounds of A. blazei, the discovery, isolation, structure, biological activity, and biosynthetic pathways of blazeispirol compounds of A. blazei were systematically reviewed. Besides, the metabolic regulation strategies related to the fermentation synthesis of blazeispirol A by A. blazei were discussed. This review could provide a reference for the efficient synthesis and development of blazeispirol compounds, the research and development of related drugs and functional foods, and the quality improvement of A. blazei and other medicinal and edible fungi resources and derivatives.
Assuntos
Agaricus , Neoplasias , Filogenia , Polissacarídeos , Esteroides , Agaricus/química , Agaricus/metabolismoRESUMO
Mushrooms are a good source of protein and phenolic compounds which provides health benefits for humans. The purpose of this study was to compare the content of eight metals, protein, and total phenolics (TPC) of 5 different species (Agaricus bisporus-white and brown mushrooms, Agaricus cupreobrunneus, Auricularia cornea, Hypsizgus tesselatus, and Pleurotus eryngii species-complex) of edible mushrooms available on the Romanian market. Agaricus bisporus and Agaricus cupreobrunneus were purchased and cultivated in Romania and the other species were cultivated in other countries (Turkey and China). The metal content determined by graphite atomic absorption spectrometry (GTAAS) varied in the order Cu > Pb > Ni > Fe > Cr > Mn > Co > Cd. Almost all the samples contained a greater quantity of metals in the stalk than in the cap. In addition, the levels of toxic metals were low. The protein content of analyzed samples ranged from 0.0926 to 0.2743%, the highest value being observed in Pleurotus eryngii species-complex mushroom. TPC of extracts increased over time but there was a variability in the concentration for each mushroom species (0.25-12.25 mg GAE/g). The investigated mushroom species possess no health risk and may be potential nutritional supplements for human diets due to their phenolic compounds, protein, and mineral content.
Assuntos
Agaricales , Agaricus , Pleurotus , Humanos , Agaricales/química , Agaricus/química , Metais/análise , Medição de RiscoRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Agaricus blazei Murill (AbM) is one of the main mushrooms used for medicinal purposes. The use of AbM in the preparation of teas is widespread mainly in Asian countries, while in Brazil it is used as a functional food to combat inflammatory diseases and cancer. AIM OF THE STUDY: The main focus of this study was the characterization of the chemical profile of the hydroalcoholic extract of Agaricus blazei Murill (AbE), as well as the evaluation of its cytotoxic and anti-inflammatory potential using human neutrophils. MATERIALS AND METHODS: The extract was prepared by dynamic maceration using a mixture of ethanol and water (70/30, v v-1) as solvent. The chemical profile characterization was carried out by 2D NMR and GC-MS techniques. The cytotoxicity of AbE was evaluated through studies of hemolytic potential, cell viability and membrane integrity. The anti-inflammatory activity was analyzed by a PMA-induced neutrophil degranulation assay. RESULTS: Chemical analysis of AbE revealed the presence of 28 metabolites in its composition, with mannitol as the major compound. AbE at 1-200 µg mL-1 and mannitol at 4-160 µg mL-1, showed low hemolytic and cytotoxic potential against human red blood cells and neutrophils. Furthermore, both were able to significantly reduce the release of myeloperoxidase. CONCLUSIONS: These results indicate that AbE is a promising natural product to be incorporated into pharmaceutical dosage forms intended for the adjuvant treatment of inflammatory diseases.
Assuntos
Agaricus , Antineoplásicos , Humanos , Neutrófilos , Cromatografia Gasosa-Espectrometria de Massas , Agaricus/química , ManitolRESUMO
Atmospheric cold plasma (ACP) is a novel nonthermal technology with potential applications in maintaining and improving food quality. The effect of ACP on the activity and structure of mushroom (Agaricus bisporus) polyphenol oxidase (PPO) was evaluated. Results demonstrated that the dielectric barrier discharge (DBD) based plasma technology could inactivate PPO (up to 69%) at 50 kV with the increased concentrations of H2O2 and NOx. An obvious enhancement of surface hydrophobicity was observed, whereas a gradual reduction of total sulfhydryl content was recorded with the increasing exposure time. Data from circular dichroism, atomic force microscopy, particle size distribution and fluorescence spectra displayed the rearrangement of secondary structure and disruption of the tertiary structure. Red shifts of fluorescence spectra showed positive correlations with the inactivation rate of PPO. Therefore, ACP treatment could be served as an alternative approach to inactivate undesirable enzymes to minimize the loss of food nutrition and quality.
Assuntos
Agaricus , Gases em Plasma , Agaricus/química , Catecol Oxidase/química , Peróxido de HidrogênioRESUMO
BACKGROUND: Button mushrooms with completely white appearance are popular with consumers. However, button mushrooms are susceptible to infection with Pseudomonas tolaasii, which results in browning. This study evaluates the effects of ultraviolet-C (UV-C) treatment on the inactivation of P. tolaasii in vitro and in vivo and on the physiological and chemical changes of button mushrooms during storage for 21 days at 4 °C. RESULTS: UV-C doses of 0.5 to 9.0 kJ m-2 resulted in 3.91-6.26 log CFU mL-1 reduction of P. tolaasii populations in vitro, and UV-C treatment reduced P. tolaasii populations inoculated on mushroom cap surfaces and browning severity. Moreover, P. tolaasii increased polyphenol oxidase (PPO) activity, and decreased phenylalanine ammonia-lyase (PAL) activity, the accumulation of phenolics and contents of brown melanin precursors, including γ-l-glutaminyl-4-hydroxybenzene (GHB), γ-l-glutaminyl-3,4-dihydroxybenzene (GDHB), and tyrosine in button mushrooms. UV-C treatment was found to reduce the negative changes due to P. tolaasii infection. CONCLUSION: These results indicated that the application of UV-C treatment inhibited browning, inactivated P. tolaasii and reduced P. tolaasii - associated chemical and enzymatic changes of button mushrooms. © 2021 Society of Chemical Industry.
Assuntos
Agaricus , Agaricus/química , Fenóis/química , PseudomonasRESUMO
BACKGROUND: Fresh mushrooms (Agaricus bisporus) are highly perishable and easily lose quality during storage. The packaging system can retard the deterioration of fresh mushrooms. However, water vapor transmission rates of commodity plastic films are lower than the transpiration rate of mushrooms, resulting in increased moisture condensation in the package and perishability of mushrooms due to microbial growth. A possible solution for controlling the humidity level in the package is to use a hygroscopic material. Acid leaching can improve the chemical and physical properties of expanded vermiculite (EV). The aim of this study was to develop a novel hygroscopic agent from acidified expanded vermiculite (AEV) and calcium chloride (CaCl2 ) that has a high moisture absorption capacity and maintains a powdered form in the packaging system for fresh mushrooms. RESULTS: Our findings revealed that leaching EV with hydrochloric acid increased porosity and hydrophilicity. The combination of AEV:CaCl2 at 6:4 (w/w, AEV/CS40) showed the highest moisture adsorption capacity at 1.724 ± 0.03 g water per gram of material. Then, 1.55 g of AEV/CS40 was applied to the mushroom packaging and stored at 4 °C. The results indicated that AEV/CS40 could delay mushroom deterioration in terms of color change, firmness, pH, total soluble solids, and microbial growth. CONCLUSION: The study findings indicate that the alternative solid hygroscopic material obtained by combining AEV with CaCl2 has potential for use as a hygroscopic material for preserving the quality of white button mushrooms. © 2021 Society of Chemical Industry.
Assuntos
Agaricus , Agaricus/química , Silicatos de Alumínio , Cloreto de Cálcio , TemperaturaRESUMO
AIDS, caused by HIV-1, is one of the most dangerous infectious diseases in the world. Therefore, it is necessary to develop new drugs with more potent bioactivities, less toxicity and higher tolerability for controlling the viral load, particularly by using the raw materials that are widely available. Agaricus blazei Murill (AbM), known in China as jisongrong, is of great importance as a food source and as a health-promoting supplement for immunomodulation. The polysaccharides of AbM exhibit various biological activities, such as regulating cellular immunity and providing anti-oxidative, anti-infective, and anti-inflammatory effects. At present, to our knowledge, no report has explored the chemically sulfated and anti-HIV-1 activity of AbM polysaccharides. Herein, the sulfated AbM polysaccharides with different sulfur contents were prepared by the chlorosulfonic acid-pyridine method. The characteristics of sulfated derivatives were established by the determination of the sulfur content, the relative molecular weight, and the Fourier-transform infrared spectroscopy. The anti-HIV activities of the sulfated AbM polysaccharides were evaluated by CCK-8 and the single-cycle pseudovirus infection (TZM-bl) assay. The sulfated AbM polysaccharides had strong antiviral properties, and the half-maximal inhibitory concentrations approached that of the positive control, azidothymidine. Sulfated modification of AbM polysaccharides can increase their anti-HIV pharmacological activity, which makes them promising alternative candidates as bioactive macromolecules for biomedical applications in HIV/AIDS.
Assuntos
Agaricus/química , Fármacos Anti-HIV/farmacologia , HIV/efeitos dos fármacos , Polissacarídeos/farmacologia , Sulfatos/química , Fármacos Anti-HIV/síntese química , Fármacos Anti-HIV/química , Configuração de Carboidratos , Testes de Sensibilidade Microbiana , Polissacarídeos/síntese química , Polissacarídeos/químicaRESUMO
The effect of Agaricus bisporus polysaccharides (ABPs) supplemented diet on growth rate, antioxidant capacity, innate-adaptive immune response, proinflammatory and antiinflammatory genes expression in Ctenopharyngodon idella against Aeromonas hydrophila is reported. In both normal and challenged groups fed with 1.0 and 1.5 mg kg-1 ABPs diets resulted in a significant weight gain and feed intake. The survival was 100% in normal fish fed without or with any ABPs diet; the challenged fish fed with 1.0 mg kg-1 ABPs diet had 98.6% survival. The RBC and WBC counts, Hb, and Hct levels were significant in both normal and challenged groups fed with 1.0 and 1.5 mg kg-1 ABPs diets. A significant increase in total protein and albumin level was observed in both groups fed with 1.0 and 1.5 mg kg-1 ABPs diets. Significant increase in GPx, ROS, GR, GSH, PC, and MnSOD activity was observed in HK of both groups fed with 1.0 and 1.5 mg kg-1 ABPs diets; similarly both groups when fed with the same ABPs diets showed significant Lz, C3, and C4 activity. However, both groups fed with 1.0 mg kg-1 ABPs diet showed significant ß-defensin, LEAP-2A, IL-6, and NF-κB P65 mRNA expression. Similarly, IFN-γ2, IL-10, and TNFα mRNA expressions were significant in both groups fed with 1.0 mg kg-1 ABPs diet. The results indicate that both normal and challenged C. idella fed with a 1.0 mg kg-1 ABPs diet had better growth, antioxidant status, immune response, and pro-anti-inflammatory gene modulation against A. hydrophila.
Assuntos
Agaricus/química , Ração Animal/análise , Carpas/metabolismo , Suplementos Nutricionais , Polissacarídeos/farmacologia , Imunidade Adaptativa/efeitos dos fármacos , Fenômenos Fisiológicos da Nutrição Animal , Animais , Anti-Inflamatórios/metabolismo , Antioxidantes/metabolismo , Dieta/veterinária , Regulação da Expressão Gênica/efeitos dos fármacos , Imunidade Inata/efeitos dos fármacos , Polissacarídeos/químicaRESUMO
Attempts to bio-enrich fungal biomass with an essential trace elements to produce dietary supplements have some tradition and an example is selenium. Lithium salts have medical applications, but safer forms are sought after, and lithiated foods and food supplements may be an alternative. This study evaluated the lithiation of white Agaricus bisporus mushrooms using commercial compost fortified with LiNO3 and investigated the effects on co-accumulation of trace elements. The fortifications at levels of 1.0, 5.0, 10, 50 and 100 mg·kg-1 dw, resulted in corresponding median increases in mushroom Li concentrations of 0.74, 5.0, 7.4, 19 and 21 mg kg-1 dw, respectively, relative to 0.031 mg kg-1 dw in control mushrooms. The bio-concentration potential for Li uptake decreased at higher levels of fortification, with saturation occurring at 100 mg·kg-1, and the level of 500 mg kg-1 mycelium failed to produce mushrooms. The compost fortification resulted in up to several hundred-fold enrichment of mushrooms compared to those grown on control compost, underlining their potential therapeutic use. At higher fortification levels, some effects were seen on the co-accumulation of other elements, such as Ag (stems), As, Cd, Cr, Cs, Cu, Hg (stems), Mn, Rb, Sr, U (stems) and Zn; 0.05 < p < 0.10), but no effects were seen for Ag (caps), Al, Ba, Co, Hg (caps) Ni, Tl, U (caps), and V (p > 0.05).
Assuntos
Agaricus/química , Compostos de Lítio/química , Lítio/análise , Nitratos/química , Oligoelementos/análise , Agaricus/metabolismo , Lítio/metabolismo , Oligoelementos/metabolismoRESUMO
Vitamin D deficiency has been linked to various diseases, but could be rectified via fortified food stuffs or supplementation. In this study 39 different hydrophobic deep eutectic solvents were evaluated for green extraction of ergosterol from mushroom. Extraction parameters (e.g. time, solvent volume) were optimized using response surface methodology (RSM) and a maximum extraction yield of 6995.00 µg ergosterol/g dry weight mushroom was attained with menthol: pyruvic acid. The extracted ergosterol was purified using a novel methodology and the extraction solvent was reused for six cycles, while retaining extraction efficiency (up to 28%). The ergosterol was exposed to ultra-violet radiation for conversion to ergocalciferol (vitamin D2) resulting in a yield of ergocalciferol that was equivalent to 2142.01 µg/g dry weight mushroom.
Assuntos
Agaricus/química , Ergosterol/isolamento & purificação , Mentol/química , Fracionamento Químico/métodos , Ergocalciferóis/química , Ergosterol/química , Indústria de Processamento de Alimentos/métodos , Interações Hidrofóbicas e Hidrofílicas , Solventes/química , Raios UltravioletaRESUMO
Selenoproteins in selenium (Se)-enriched vegetables play an important role in human health. In this study, three water-soluble selenoproteins PR-Se-1, PR-Se-2 and PR-Se-3 in Agaricus blazei Murrill (ABM) were isolated by anion exchange chromatography, gel filtration chromatography and SDS-PAGE. Sequence analyses performed by HPLC-MS/MS showed that PR-Se-1, a 114024 Da selenoprotein with 1019 amino acids (AAs), is an isoenzyme of isocitrate dehydrogenase. PR-Se-2, a 53983 Da selenoprotein with 508 AAs, is a kind of dihydrolipoyl dehydrogenase. PR-Se-3, a 47179 Da selenoprotein with 415 AAs, is a kind d-proline reductase. Se content is high at 26.1 µg/g, and selenocystine is the predominant Se unit in the three selenoproteins. Se content of ABM is 9.15 µg/g, and the organic form of Se accounts for ~81% of total Se content. ABM could be a promising source of Se in Se-poor regions.
Assuntos
Agaricus/química , Selênio/química , Selenoproteínas/análise , Agaricus/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Selênio/metabolismo , Selenoproteínas/isolamento & purificação , Espectrometria de Massas em Tandem , Água/químicaRESUMO
BACKGROUND: The potential of onion juice, as well as extracts of waste (tunic) (5%) and fleshy scale leaves (25%), to inhibit enzymatic browning of frozen Agaricus bisporus was investigated. The onion materials were used for blanching and their effectiveness in conserving integrity and appearance of mushroom fruiting bodies was compared with the currently accepted method of blanching in a sodium metabisulfite (SM) solution. RESULTS: It was observed that l-phenylalanine content may be a useful indicator of the changes in enzymatic activity during frozen storage, and l-tyrosine may be an indicator of a loss of lightness in color (parameter L*). The enzymes responsible for color changes were mainly monophenolase (MON) and, to a lesser degree, diphenolase (DIP). After being stored frozen for 8 months, these enzymes were detected at a 29:1 (DIP:MON) ratio in untreated mushrooms and a 2:1 (DIP:MON) ratio in mushrooms treated with onion juice. CONCLUSION: Onion products may be a good alternative to an SM solution. The most effective method to conserve the light color of fruiting bodies was blanching in juice or in an extract of the fleshy scale leaves. The least effective inhibitor of MON was tunic extract, which did, however, cause a favourable increase in the reducing capacity (total polyphenols) and flavonoids. Although the onion waste (tunic) extract changed the color of mushrooms from white to creamy orange, the color of these products was attractive and positively evaluated by panellists. © 2020 Society of Chemical Industry.
Assuntos
Agaricus/enzimologia , Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Proteínas Fúngicas/metabolismo , Cebolas/química , Extratos Vegetais/farmacologia , Agaricus/química , Agaricus/efeitos dos fármacos , Cor , Proteínas Fúngicas/química , Sulfitos/farmacologiaRESUMO
There is evidence from both in vitro and animal models that the consumption of edible mushrooms has beneficial effects on health. It is unclear whether similar effects exist in humans and which bioactive compounds are present. This review synthesises the evidence on the world's most commonly consumed mushroom, Agaricus bisporus to (i) examine its effect on human health outcomes; and (ii) determine the nutrient density of its bioactive compounds, which may explain their health effects. A systematic literature search was conducted on the consumption of A. bisporus, without date and study design limits. Bioactive compounds included ergosterol, ergothioneine, flavonoids, glucans and chitin. Two authors independently identified studies for inclusion and assessed methodological quality. Beneficial effects of A. bisporus on metabolic syndrome, immune function, gastrointestinal health and cancer, with the strongest evidence for the improvement in Vitamin D status in humans, were found. Ultraviolet B (UVB) exposed mushrooms may increase and maintain serum 25(OH)D levels to a similar degree as vitamin D supplements. A. bisporus contain beta-glucans, ergosterol, ergothioneine, vitamin D and an antioxidant compound usually reported as flavonoids; with varying concentrations depending on the type of mushroom, cooking method and duration, and UVB exposure. Further research is required to fully elucidate the bioactive compounds in mushrooms using vigorous analytical methods and expand the immunological markers being tested. To enable findings to be adopted into clinical practice and public health initiatives, replication of existing studies in different population groups is required to confirm the impact of A. bisporus on human health.
Assuntos
Agaricus , Produtos Biológicos/farmacologia , Agaricus/química , Animais , Produtos Biológicos/química , Produtos Biológicos/uso terapêutico , Ergosterol/química , Ergosterol/farmacologia , Ergosterol/uso terapêutico , Ergotioneína/química , Ergotioneína/farmacologia , Ergotioneína/uso terapêutico , Flavonoides/química , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Glucanos/química , Glucanos/farmacologia , Glucanos/uso terapêutico , HumanosRESUMO
Since the 1980s, medicinal effects have been documented in scientific studies with the related Basidiomycota mushrooms Agaricus blazei Murill (AbM), Hericium erinaceus (HE) and Grifola frondosa (GF) from Brazilian and Eastern traditional medicine. Special focus has been on their antitumor effects, but the mushrooms' anti-inflammatory and antiallergic properties have also been investigated. The antitumor mechanisms were either direct tumor attack, e.g., apoptosis and metastatic suppression, or indirect defense, e.g., inhibited tumor neovascularization and T helper cell (Th) 1 immune response. The anti-inflammatory mechanisms were a reduction in proinflammatory cytokines, oxidative stress and changed gut microbiota, and the antiallergic mechanism was amelioration of a skewed Th1/Th2 balance. Since a predominant Th2 milieu is also found in cancer, which quite often is caused by a local chronic inflammation, the three conditions-tumor, inflammation and allergy-seem to be linked. Further mechanisms for HE were increased nerve and beneficial gut microbiota growth, and oxidative stress regulation. The medicinal mushrooms AbM, HE and GF appear to be safe, and can, in fact, increase longevity in animal models, possibly due to reduced tumorigenesis and oxidation. This article reviews preclinical and clinical findings with these mushrooms and the mechanisms behind them.
Assuntos
Agaricus/química , Antialérgicos , Anti-Inflamatórios , Antineoplásicos , Basidiomycota/química , Produtos Biológicos/isolamento & purificação , Produtos Biológicos/farmacologia , Grifola/química , Hericium/química , Animais , Produtos Biológicos/uso terapêutico , Citocinas/metabolismo , Modelos Animais de Doenças , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos , Mediadores da Inflamação/metabolismo , Camundongos , Neoplasias/irrigação sanguínea , Neoplasias/tratamento farmacológico , Neovascularização Patológica , Estresse Oxidativo/efeitos dos fármacos , Ratos , Linfócitos T Auxiliares-Indutores/imunologiaRESUMO
Depression is the most common form of mental illness and the major cause of disability worldwide. Symptoms of depression, including feelings of intense sadness and hopelessness, may occur after a specific event or in response to a gradual decline in health and functional status, often associated with aging. Current therapies for treating these symptoms include antidepressant drugs, counseling and behavioral therapy. However, antidepressant drugs are associated with mild to severe adverse effects, which has prompted the need for better treatment options. Medicinal mushrooms are valuable sources of food and medicine and are increasingly being used as supplements or as alternative medicines in standard healthcare. Numerous studies have provided insights into the neuroprotective effects of medicinal mushrooms, which are attributed to their antioxidant, anti-neuroinflammatory, cholinesterase inhibitory and neuroprotective properties. In this review, we comprehensively examine the role of these medicinal mushrooms in the treatment of depression. However, to apply these natural products in clinical settings, the therapeutic agent needs to be properly evaluated, including the active ingredients, the presence of synergistic effects, efficient extraction methods, and stabilization of the active ingredients for delivery into the body as well as crossing the blood-brain barrier.
Assuntos
Agaricales/química , Agaricales/metabolismo , Agaricus/química , Agaricus/metabolismo , Animais , Antidepressivos/química , Antidepressivos/isolamento & purificação , Antidepressivos/farmacologia , Antidepressivos/uso terapêutico , Depressão/tratamento farmacológico , Depressão/patologia , Humanos , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Medicina Tradicional , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêuticoRESUMO
We investigated whether outdoor-cultivated Agaricus brasiliensis (KA21) could reduce the side effects caused by the anticancer medicine 5-fluorouracil (5-FU). The adverse effects of 5-FU were analyzed in mice by orally administering the drug every day for 5 days. Leukopenia, diarrhea, body weight loss, anorexia, kidney injury, gastrointestinal tract injury, and hair loss were evaluated as side effects. We determined whether these side effects were reduced by the intake of outdoor-cultivated A. brasiliensis. The side effects were reduced in mice receiving the outdoor-cultivated A. brasiliensis but not in those receiving the indoor-cultivated A. brasiliensis. These results suggest that outdoor-cultivated A. brasiliensis is beneficial in reducing the side effects of the anticancer medicine and might, therefore, be useful in improving the quality of life of patients with cancer. Furthermore, because antioxidants have been reported to contribute to the suppression of the side effects of anticancer medicines, the antioxidant activities of different mushrooms were determined. The antioxidant activity of the outdoor-cultivated A. brasiliensis was the highest among all the tested mushrooms. These findings make it clear that the cultivation conditions of A. brasiliensis are important in suppressing the side effects of anticancer medicines. Strong antioxidant activity might be one of the mechanisms through which this pharmacological activity is mediated.
Assuntos
Agaricus/química , Antineoplásicos/efeitos adversos , Antioxidantes/química , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/prevenção & controle , Agricultura , Animais , Feminino , Fluoruracila/efeitos adversos , Camundongos , Camundongos Endogâmicos BALB CRESUMO
The mushroom Agaricus bisporus secretes biologically active compounds and proteins with benefits for human health. Most reported proteins from A. bisporus are tyrosinases and lectins. Lectins are of therapeutic or pharmaceutical interest. To date, only limited information is available on A. bisporus lectins and lectin-like proteins. No therapeutic products derived from A. bisporus lectin (ABL) are available on the market despite its extensive exploration. Recently, A. bisporus mannose-binding protein (Abmb) was discovered. Its discovery enriches the information and increases the interest in proteins with therapeutic potential from this mushroom. Furthermore, the A. bisporus genome reveals the possible occurrence of other lectins in this mushroom that may also have therapeutic potential. Most of these putative lectins belong to the same lectin groups as ABL and Abmb. Their relationship is discussed. Particular attention is addressed to ABL and Abmb, which have been explored for their potential in medicinal or pharmaceutical applications. ABL and Abmb have anti-proliferative activities toward cancer cells and a stimulatory effect on the immune system. Possible scenarios for their use in therapy and modification are also presented.
Assuntos
Agaricus/química , Lectinas/genética , Lectina de Ligação a Manose/genética , Monofenol Mono-Oxigenase/genética , Agaricus/genética , Genoma Fúngico/genética , Humanos , Lectinas/uso terapêutico , Lectina de Ligação a Manose/química , Lectina de Ligação a Manose/uso terapêutico , Monofenol Mono-Oxigenase/químicaRESUMO
Agaricus bisporus mushrooms are well known for their nutritional and medicinal values. A. bisporus is a source of protein (about 40% on a dry basis), ergosterol, several minerals, carbohydrate, and fat. The present study was conducted to investigate the effect of A. bisporus S-II extracts on human pathogenic bacteria in-vitro condition. Totally, three human pathogenic bacterial strains (MTCC culture type) were procured from the Institute of Microbial Technology, India. Out of these three bacterial strains, one was Gram-negative (namely P. aeruginosa MTCC741), and the other two were Gram-positive (B. cereus MTCC9786 and S. aureus MTCC740). Microdilution assay was applied for the evaluation of the minimum inhibitory concentration (MIC). The highest antimicrobial activity was observed in methanol extract (26.5%) against S. aureus MTCC740, compared to ethanol extract (17%). Similar results were obtained for P. aeruginosa MTCC741 (21.8%) and B. cereus MTCC9786 (15%) in methanol extract. Least microbial growth inhibition observed for B. cereus MTCC9786 (13.82%) followed by P. aeruginosa MTCC741 (14%), compared to control in ethanol extract. The highest antimicrobial activity up to 17% with ethanolic extracts recorded against S. aureus MTCC740. The MIC results in microtitre plates showed the growth inhibition of P. aeruginosa MTCC741 and S. aureus MTCC740 at extract concentrations of 15 mg/ml and 20 mg/ml, respectively. However, no MIC detected for B. cereus MTCC9786 below 20 mg/ml extract concentration. Regarding minimum bactericidal concentration, the bactericidal value for P. aeruginosa MTCC741 and S. aureus MTCC740 was obtained at 10 mg/ml concentration. The present study indicated that the extracts of the A. bisporus S-II mushrooms had promising antimicrobial activities against the tested organisms.
Assuntos
Agaricus/química , Bacillus cereus/efeitos dos fármacos , Agentes de Controle Biológico/farmacologia , Extratos Vegetais/farmacologia , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Humanos , Testes de Sensibilidade MicrobianaRESUMO
Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively). UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 nm for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, 0.97 µg/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 µg/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 µg/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection.
Assuntos
Agaricus/química , Anti-Infecciosos/química , Basidiomycota/química , Polissacarídeos Fúngicos/química , Nanopartículas Metálicas/química , Prata/química , Anti-Infecciosos/farmacologia , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/crescimento & desenvolvimento , Prata/farmacologiaRESUMO
Agaricus bisporus is well known for its nutraceutical properties. To meet consumer market demand, there is an urgent need for new strains with disease resistance and a diverse nutrient profile for commercial cultivation. Wild germplasm resources provide a good source for the breeding of new strains for this purpose. In this study, we evaluated the physical, chemical, and structural properties of wild domesticated (CCMJ1351) and major commercially cultivated strains (CCMJ1013, CCMJ1028, and CCMJ1343) of A. bisporus from China. The results showed significant differences among the strains for all parameters measured. In terms of morphological characteristics, CCMJ1351 possessed the highest stipe thickness, fruiting body individual weight, cohesiveness, and springiness; CCMJ1013 demonstrated maximum pileus diameter and thickness; CCMJ1028 exhibited the highest textural hardness and color characteristics; and strain CCMJ1343 had the highest yield. CCMJ1351 ranked top among all the strains for proximate composition, rheological profile, and structural and mechanical properties, containing 21.93% crude protein and the highest dry matter, crude fat, and fiber contents. However, the bioactive chemical constituents present in the four strains were very similar, especially ß-(1â3)-glucan, according to Fourier transform infrared spectroscopy analysis, while some minimal peaks varied among the different strains. Therefore, in combination with previously identified high disease-resistance traits, the wild domesticated strain CCMJ1351 constitutes a good candidate for further exploitation in breeding programs and is suitable for fresh consumption as well as incorporation into various food products.