Factors affecting the fate of nanoencapsulates post administration.

Nanoencapsulation has found numerous applications in the food and nutraceutical industries. Micro and nanoencapsulated forms of bioactives have proven benefits in terms of stability, release, and performance in the body. However, the encapsulated ingredient is often subjected to a wide range of processing conditions and this is followed by storage, consumption, and transit along the gastrointestinal tract. A strong understanding of the fate of nanoencapsulates in the biological system is mandatory as it provides valuable insights for ingredient selection, formulation, and application. In addition to their efficacy, there is also the need to assess the safety of ingested nanoencapsulates. Given the rising research and commercial focus of this subject, this review provides a strong focus on their interaction factors and mechanisms, highlighting their prospective biological fate. This review also covers various approaches to studying the fate of nanoencapsulates in the body. Also, with emphasis on the overall scope, the need for a new advanced integrated common methodology to evaluate the fate of nanoencapsulates post-administration is discussed.

Effect of different drying methods on the functional properties of probiotics encapsulated using prebiotic substances.

Probiotics and prebiotics together work synergistically as synbiotics and confer various health benefits. Many studies on synbiotic foods only focus on the survival of probiotics but fail to evaluate their functional properties. The impact on functional properties should be explored to better understand its therapeutic efficacy. In this work, probiotics (Lactiplantibacillus plantarum NCIM 2083) were encapsulated with prebiotics (fructooligosaccharide + whey protein + maltodextrin) using spray-drying (SD), freeze-drying (FD), spray-freeze-drying (SFD), and refractance window-drying (RWD) techniques. Aggregation, intestinal adhesion, antagonistic activity, and bile salt hydrolase (BSH) activity of probiotics were studied before and after the encapsulation process. The SFD probiotics showed better aggregation ability (79% at 24-h incubation), on par with free cells (FC) (81% at 24-h incubation). The co-aggregation ability of encapsulated probiotics has drastic variations with each pathogenic strain. The adhesion ability of probiotics in chicken intestinal mucus was assessed by the crystal violet method, indicating no significant variations between FC and SFD probiotics. Also, encapsulated probiotics exhibit antagonistic activity (zone of inhibition in mm) against gut pathogens E. coli (11.33 to 17.34), S. faecalis (8.83 to 15.32), L. monocytogenes (13.67 to 18), S. boydii (12.17 to 15.5), and S. typhi (2.17 to 6.86). Overall, these studies confirm the significance and impact of various drying techniques on the functionality of encapsulated probiotics in synbiotic powders. KEY POINTS: • Understanding the relevance of processing effects on the functionality of probiotics. • Spray-freeze-dried probiotics showed superior functional properties. • The encapsulation process had no significant impact on bile salt hydrolase activity.

Development of iron-vitamin multilayer encapsulates using 3 fluid nozzle spray drying.

Considering the growing concern of iron and folic acid deficiency, encapsulation of these nutrients and fortification into foods is emerging as an effective counter-strategy. The present work focuses on a scalable approach for the production of iron, ascorbic acid, and folic acid core-shell encapsulates using novel 3-fluid nozzle (3FN) spray drying with whey protein as core and either pectin or hydroxypropyl methylcellulose (HPMC) as shell polymers. The effect of shell formation was observed by comparing core-shell encapsulates with conventional 2-fluid nozzle (2FN) encapsulates. Also, the effect of pH of whey protein on the color of encapsulates is noteworthy; reducing the pH to 4.0 significantly improved the lightness value (52.91 ± 0.13) when compared with the encapsulates with native pH (38.91 ± 0.58). Furthermore, sample with pectin as shell polymer exhibited fair flowability with lowest values of Hausner ratio (1.25 ± 0.04) and Carr's index (20.06 ± 2.71) and highest encapsulation efficiency for folic acid (86.07 ± 5.24%). Whereas, encapsulates having HPMC as shell polymer showed highest lightness value (60.80 ± 0.32) and highest encapsulation efficiency for iron (87.28 ± 4.15%). The formation of core-shell structure was confirmed by evaluation of the surface composition which showed reduced amine bonds and increased aliphatic and carbonyl bonds in the encapsulates prepared by 3FN spray drying. The encapsulates prepared without adjusting whey protein pH showed the least release (∼51 % in 24 h) and bioaccessibility (∼56%) of iron indicating the iron-whey protein complex formation. Based on appearance, smooth surface morphology, flowability, and release behavior, a combination of whey protein and pectin is recommended for co-encapsulation of iron, folic acid and ascorbic acid.

A dynamic in vitro oral mastication system to study the oral processing behavior of soft foods.

A bolus-oriented artificial oral mastication system was developed to simulate the dynamics of food mastication in the human mouth. The system consists of a chewing unit, a bolus forming unit, and provisions for the dynamic incorporation of saliva during mastication. The system performance was validated with in vivo trials (n = 25) considering time-dependent changes in particle size, textural attributes and rheological behavior of the bolus. Idli, a fermented and steamed black gram-rice-based Indian food was considered the model soft food for all trials measured in triplicates. The mastication dynamics were evaluated by analyzing bolus properties during every 3 s of mastication. Large strain shear rheology tests revealed that the viscosity of the sample decreased over time. Results of in vivo trials follow close trends in particle size and rheological behavior and have no significant change in correlation with in vitro mastication results. Similar observations were made in the half softening time of idli during mastication as determined using the relative change in hardness (hardness ratio (Ht/H0)) values fitted to the Weibull model. Also, a model to simulate the time-dependent changes in bolus adhesiveness was developed.

Food waste valorisation via gasification - A review on emerging concepts, prospects and challenges.

Disposing of the enormous amounts of food waste (FW) produced worldwide remains a great challenge, promoting worldwide research on the utilization of FW for the generation of value-added products. Gasification is a significant approach for decomposing and converting organic waste materials into biochar, bio-oil, and syngas, which could be adapted for energy (hydrogen (H2) and heat) generation and environmental (removal of pollutants and improving the soil quality) applications. Employment of FW matrices for syngas production through gasification is one of the effective methods of energy recovery. This review explains different gasification processes (catalytic and non-catalytic) used for the decomposition of unutilized food wastes and the effect of operating parameters on H2-rich syngas generation. Also, potential applications of gasification byproducts such as biochar and bio-oil for effective valorization have been discussed. Besides, the scope of simulation to optimize the gasification conditions for the effective valorization of FW is elaborated, along with the current progress and challenges in the research to identify the feasibility of gasification technology for FW. Overall, this review concludes the sustainable route for conversion of unutilized food into hydrogen-enriched syngas production.

3D printed MCT oleogel as a co-delivery carrier for curcumin and resveratrol.

Designing a suitable matrix to protect sensitive bioactive compounds is an important stage in nutraceutical development. In this study, emulsion templated medium-chain triglycerides (MCT) oleogel was developed as co-delivery carriers for synergistic nutraceuticals, curcumin, and resveratrol and to 3D print in customized shapes for personalized nutrition. To obtain the stable emulsion, gelatin and gellan gum were added such that their protein-polysaccharide interaction helps in the structuring of the oil phase. Increasing the amount of gellan gum had a positive effect on stabilizing the emulsion but became the critical parameter during 3D printing. Hence, gellan gum of 1.5% (w/v) and gelatin at 10% (w/v) of water were considered optimum to produce a stable 30% O/W emulsion for 3D printing. Upon analyzing the in-vitro digestion behavior of the oleogel, it was observed that the bioactives were protected under oral and gastric conditions and allowed intestinal targeted delivery. The total bioaccessible fraction increased up to 1.13-fold and 1.2-fold for curcumin and resveratrol respectively compared to control (MCT oil). The FFAs release profile also indicated that gelators play an important role in lipase activity. Also, the ex-vivo everted gut sac analysis showed enhanced permeation of about 1.83 times and 1.13 times for curcumin and resveratrol respectively. Thus, this study provides useful insights into the 3D printing of emulsion templated oleogel as personalized nutraceutical carriers.

Co-electrospun-electrosprayed ethyl cellulose-gelatin nanocomposite pH-sensitive membrane for food quality applications.

Anthocyanin from red cabbage is an important biomolecule suitable for pH sensing due to its oxidoreduction potential that leads to a color change at various pH conditions. The pH-sensitive anthocyanin compound was extracted from red cabbage (1785 ± 235 mg/L) and encapsulated with gelatin as the wall material at the nanoscale (350 nm) through electrospraying. By using a simultaneous electrospraying and spinning process at 20 kV, nanoencapsulated anthocyanin was immobilized on ethyl cellulose (EC) nanofibers and formed as a nanocomposite membrane. The surface morphology of developed nanocomposites has shown complex nonwoven nanofiber formation and the immobilized nano encapsulates captured inside the nanofibrous membrane. The pH sensitivity was significantly stable up to 7 days of storage at room temperature. Total color difference has been observed to be statistically significant at different pH conditions of 2 to 12. Also, the application of the nanocomposite strips in pH sensing during milk spoilage was studied and reported.

Resource recovery from fish waste: Prospects and the usage of intensified extraction technologies.

Globally, the valorization of fish biowaste as a feedstock to recover valuable components is an emerging research and commercial interest area to achieve the SDG goals by 2030. Fish waste-derived biomolecules are increasingly finding diverse applications in food and other biotechnological fields due to their excellent chemical, structural and functional properties. The focus of this review is to highlight the conventional valorization routes and recent advancements in extraction technologies for resource recovery applications, primarily focusing on green processes. Biointensified processes involving ultrasound, microwave, sub- and supercritical fluids, pulsed electric field, high-pressure processing, and cold plasma are extensively explored as sustainable technologies for valorizing fish discards and found numerous applications in the production of functional and commercially important biomaterials. With challenges in recovering intracellular bioactive compounds, selectivity, and energy requirement concerns, conventional approaches are being relooked continuously in the quest for process intensification and sustainable production practices. Nonetheless, in the context of 'zero waste' and 'biorefinery for high-value compounds', there is immense scope for technological upgradation in these emerging alternative approaches. This work details such attempts, providing insights into the immense untapped potential in this sector.

Novel powder-XRD method for detection of acrylamide in processed foods.

Acrylamide (AA) is a toxin in food systems associated with neurotoxicity, carcinogenicity, genotoxicity, and cardiotoxicity. While several approaches exist, this work details the first-of-its-kind application of Powder X-Ray Diffraction (PXRD) for the detection of AA in food systems. A new methodology was validated as per ICH guidelines. Various x-ray tube combinations such as current (25-45 mA), voltage (25-45 kV), and scanning parameters such as step size (0.006-0.10°) and time-per-step (50-150 s) were experimented on 10-80° angle 2theta (2θ). In this investigation, more diffracted peaks were detected on time per step 151.210 [s], 0.0135[°] step size @ 35 mA/45 kV through phase quantification. The developed method was successfully applied to real processed food samples for qualitative analysis of AA. In addition, the influence of oil frying temperature-time conditions on AA content was studied. Overall, this work highlights the prospects of PXRD-based food quality and safety evaluation.

Conventional and emerging approaches for reducing dietary intake of salt.

High dietary intake of sodium is associated with several non-communicable diseases and depleting immunity. In recent years, with emergent regulations and increasing awareness about the role of diet on human health, there has been significant research and commercial interest in salt reduction approaches. Nevertheless, lowering the level of salt while maintaining the physicochemical properties, sensory attributes and microbial stability of food products is a challenging task for the food industry. This review analyses scientific literature and details the various techniques that have been used for the effective reduction of sodium levels in food products. These include various physical and chemical modifications, apart from psychological approaches, and novel non-thermal processing techniques. The work presents a comprehensive understanding of recent advancements in lowering salt content, with implications on their effects on the properties and consumer acceptance of foods. Some approaches have been proven to be realistic and sustainable. Importantly, a combination of different strategies can overcome the drawbacks of conventional methods and facilitate the development of high-quality foods. However, numerous challenges need to be addressed for the production of shelf-stable low-salt products. Future research works must consider these novel concepts and focus on commercial-level applications.

Medium chain triglycerides (MCT): State-of-the-art on chemistry, synthesis, health benefits and applications in food industry.

Medium chain triglycerides (MCT) are esters of fatty acids with 6 to 12 carbon atom chains. Naturally, they occur in various sources; their composition and bioactivity are source and extraction process-linked. The molecular size of MCT oil permits unique metabolic pathways and energy production rates, making MCT oil a high-value functional food. This review details the common sources of MCT oil, presenting critical information on the various approaches for MCT oil extraction or synthesis. Apart from conventional techniques, non-thermal processing methods that show promising prospects are analyzed. The biological effects of MCT oil are summarized, and the range of need-driven modification approaches are elaborated. A section is devoted to highlighting the recent trends in the application of MCT oil for food, nutraceuticals, and allied applications. While much is debated about the role of MCT oil in human health and wellness, there is limited information on daily requirements, impact on specific population groups, and effects of long-term consumption. Nonetheless, several studies have been conducted and continue to identify the most effective methods for MCT oil extraction, processing, handling, and storage. A knowledge gap exists and future research must focus on technology packages for scalability and sustainability.

Impact of processing techniques on the glycemic index of rice.

Rice is an important starchy staple food and generally, rice varieties are known to have a higher glycemic index (GI). Over the years, the significance of GI on human health is being better understood and is known to be associated with several lifestyle disorders. Apart from the intrinsic characteristics of rice, different food processing techniques are known to have implications on the GI of rice. This work details the effect of domestic and industrial-level processing techniques on the GI of rice by providing an understanding of the resulting physicochemical changes. An attempt has been made to relate the process-dependent digestion behavior, which in turn reflects on the GI. The role of food constituents is elaborated and the various in vitro and in vivo approaches that have been used to determine the GI of foods are summarized. Considering the broader perspective, the effect of cooking methods and additives is explained. Given the significance of the cereal grain, this work concludes with the challenges and key thrust areas for future research.

Determining the glycaemic responses of foods: conventional and emerging approaches.

A low-glycaemic diet is crucial for those with diabetes and cardiovascular diseases. Information on the glycaemic index (GI) of different ingredients can help in designing novel food products for such target groups. This is because of the intricate dependency of material source, composition, food structure and processing conditions, among other factors, on the glycaemic responses. Different approaches have been used to predict the GI of foods, and certain discrepancies exist because of factors such as inter-individual variation among human subjects. Besides other aspects, it is important to understand the mechanism of food digestion because an approach to predict GI must essentially mimic the complex processes in the human gastrointestinal tract. The focus of this work is to review the advances in various approaches for predicting the glycaemic responses to foods. This has been carried out by detailing conventional approaches, their merits and limitations, and the need to focus on emerging approaches. Given that no single approach can be generalised to all applications, the review emphasises the scope of deriving insights for improvements in methodologies. Reviewing the conventional and emerging approaches for the determination of GI in foods, this detailed work is intended to serve as a state-of-the-art resource for nutritionists who work on developing low-GI foods.

Mucilages: sources, extraction methods, and characteristics for their use as encapsulation agents.

The increasing interest in the use of natural ingredients has driven keen research and commercial interest in the use of mucilages for a range of applications. Typically, mucilages are polysaccharide hydrocolloids with distinct physicochemical and structural diversity, possessing characteristic functional and health benefits. Apart from their role as binding, thickening, stabilizing, and humidifying agents, they are valued for their antimicrobial, antihypertensive, antioxidant, antiasthmatic, hypoglycemic, and hypolipidemic activities. The focus of this review is to present the range of mucilages that have been explored as encapsulating agents. Encapsulation of food ingredients, nutraceutical, and pharmaceutical ingredients is an attractive technique to enhance the stability of targeted compounds, apart from providing benefits on delivery characteristics. The most widely adopted conventional and emerging extraction and purification methods are explained and supplemented with information on the key criteria involved in characterizing the physicochemical and functional properties of mucilages. The unique traits and benefits of using mucilages as encapsulation agents are detailed with the different methods used by researchers to encapsulate different food and bioactive compounds.

Targeted Delivery of Probiotics: Perspectives on Research and Commercialization.

Considering the significance of the gut microbiota on human health, there has been ever-growing research and commercial interest in various aspects of probiotic functional foods and drugs. A probiotic food requires cautious consideration in terms of strain selection, appropriate process and storage conditions, cell viability and functionality, and effective delivery at the targeted site. To address these challenges, several technologies have been explored and some of them have been adopted for industrial applicability. Encapsulation of probiotics has been recognized as an effective way to stabilize them in their dried form. By conferring a physical barrier to protect them from adverse conditions, the encapsulation approach renders direct benefits on stability, delivery, and functionality. Various techniques have been explored to encapsulate probiotics, but it is noteworthy that the encapsulation method itself influences surface morphology, viability, and survivability of probiotics. This review focuses on the need to encapsulate probiotics, trends in various encapsulation techniques, current research and challenges in targeted delivery, the market status of encapsulated probiotics, and future directions. Specific focus has been given on various in vitro methods that have been explored to better understand their delivery and performance.

Prediction of in-vitro glycemic responses of biscuits in an engineered small intestine system.

The Glycemic Index (GI) indicates the effect of a food product on the increase/decrease in postprandial blood glucose levels. Monitoring the glycemic response is important, as it is associated with the human nutrition absorption. However, human or animal blood sampling involves financial, ethical, and other challenges. Accordingly, in the recent past, many human digestion models have been developed to understand the intricate changes that occur during human digestion and absorption processes. Dynamic models help in studying the digestion mechanics and hypotheses related to the health effects of food ingredients in controlled conditions. The objective of this study is to predict the GI of different biscuit composition using an engineered small intestine system, considering a mass transfer approach and in-silico simulation. Different GI was predicted for biscuits: 62 for plain biscuits (N) containing only refined wheat flour; 57 for finger millet flour biscuits (F); 51 for wheat bran fortified biscuits (W). The results were close to human in-vivo values; GI of 76 for plain biscuits (N); 58 for finger millet biscuits (F) and 41 for wheat bran fortified (W) biscuits, respectively (with no significant difference in the GI values of Engineered model and human glucose blood sampling results) which was confirmed with Bland-Altmann statistics. This finding will help in predicting the glycemic response of new biscuit products serving as an alternative approach to human blood sampling for GI analysis.

Effect of conductive hydro-drying on physiochemical and functional properties of two pulse protein extracts: Green gram (Vigna radiata) and black gram (Vigna mungo).

In this study, the effect of two drying methods (conductive hydro-drying - CHD and freeze-drying - FD) on the physical and functional properties of green gram (GG) and black gram (BG) protein powders was investigated. CHD dried protein powder showed excellent powder characteristics with moisture contents ranging from 3 to 6%, water activity of ~0.4 and Carr index ≤10. The CHD samples were dried in 210 min; with higher drying rates, CHD samples showed no significant changes in powder characteristics, color value, and water and oil absorption indices. The solubility of both proteins were found to be lower at pH 4 to 7 and higher at pH 1, 2, 8, 9 and 10; at certain pH, the solubility of CHD protein was higher than that of the FD counterparts. No significant differences were observed in the oil absorption capacity, surface hydrophobicity, protein gel formation, pasting and thermal properties. XRD and FTIR analyses were used to explain changes in protein structure and the presence of both α-helix and ß-sheet was observed, with higher ß-sheet levels in both pulses dried using CHD. Results confirmed that CHD, a variant of the refractance window drying (RWD) offered protein quality in par with FD.

Total conjugated linoleic acid content of ruminant milk: The world status insights.

Conjugated linoleic acid (CLA) content of ruminant milk reported in published research papers (n = 65) from January 1995 to March 2020 around the world were analyzed to estimate the overall mean CLA value. The CLA content of ruminant milk samples was grouped according to geographical regions (Europe, South America, North America, Oceania, Asia, and Africa). The total CLA content of milk samples from cows, sheep, goats, yaks, and llama retrieved from the collected data ranged between 0.06 and 2.96% of total fatty acids. There is a wide variation of pooled estimated mean content of CLA in milk among the study regions and were highest in Oceania with 1.33% (95% confidence interval (CI): 1.16 - 1.49%) of total fatty acids. Though several factors have been reported to influence the CLA content of milk, the effect of the "geographical origin" was only considered in the present manuscript as one of the main factors in this respect.

Synergistic potential of nutraceuticals: mechanisms and prospects for futuristic medicine.

Nutraceuticals are valued for their therapeutic effects and numerous health benefits. In recent years, several studies have demonstrated their superior performances when co-delivered; the concept of synergism has been established for various bioactives. Apart from improvements in the bioavailability of partnering compounds, this approach can protect the radical scavenging potential and biological effects of individual compounds. In this review, the intricate mechanisms that promote synergistic effects when bioactive compounds are co-delivered are detailed. Importantly, a range of potential medical applications that have been established through such synergistic effects is presented, emphasizing recent developments in this field. Also, a section has been devoted to highlighting perspectives on co-encapsulation at the nanoscale for improved synergistic benefits. While prospects for the treatment of chronic diseases are well-demonstrated, several challenges and safety concerns remain, and these have been discussed, providing recommendations for future research.

Utilization of food waste streams for the production of biopolymers.

Uncontrolled decomposition of agro-industrial waste leads to extensive contamination of water, land, and air. There is a tremendous amount of waste from various sources which causes serious environmental problems. The concern in the disposal problems has stimulated research interest in the valorization of waste streams. Valorization of the wastes not only reduces the volume of waste but also reduces the contamination to the environment. Waste from food industries has great potential as primary or secondary feedstocks for biopolymer production by extraction or fermentation with pre-treatment or without pre-treatment by solid-state fermentation to obtain fermentable sugars. Various types of waste can be used as substrates for the production of biomaterials but recently more focus has been observed on the agro-industrial wastes which have a high rate of production worldwide. This review collates in detail the different food wastes used for biopolymer, technologies for the production and characterization of the biopolymers, and their economic/technical viability.