RESUMEN
Clinical evaluation of a young woman with subcutaneous fibrotic nodules, progressive distal joint contractures and marfanoid stature revealed a previously unrecognized fibrotic disorder characterized by several unique phenotypic features and some features overlapping with known disorders. Mutational analysis of the FBN1 and FBN2 genes excluded Marfan syndrome and congenital contractural arachnodactyly. MMP2 gene sequence analysis excluded multicentric osteolysis, nodulosis and arthropathy. The lack of mutations within the MAGP2 gene also excluded an MAGP2-associated disorder. In order to establish the mechanistic basis for the severe skin pathology noted in this patient, we performed transcriptional profiling of dermal fibroblasts, and candidate gene expression studies in conjunction with immunocytochemistry and cell-based and functional assays. Data from these experiments have further excluded any previously recognized fibrotic disorder and identified a unique pattern of gene expression in this patient consistent with progressive fibrosis. The pathogenic mechanisms included persistent proliferation of dermal fibroblasts in coexistence with a disarray of the microfibrillar network. Collagen accumulation, moreover, could be linked to extensive crosslinking resulting from increased activities of lysyl oxidases (LOX and LOXL), and lack of remodelling due to deficiencies in collagenolytic matrix metalloproteinases. The disorder may represent a novel syndrome in which transforming growth factor-ß1-independent dermal fibrosis, unlike known microfibrillar disorders caused by single gene deficiencies, associates with a disarray of the microfibrillar network.
Asunto(s)
Dermis/patología , Fibroblastos/metabolismo , Fibroblastos/patología , Fibrosis/diagnóstico , Fibrosis/genética , Adulto , Biopsia , Proliferación Celular , Proteínas Contráctiles/genética , Proteínas Contráctiles/metabolismo , Citocinas/metabolismo , Análisis Mutacional de ADN , Dermis/ultraestructura , Regulación hacia Abajo , Proteínas de la Matriz Extracelular/metabolismo , Femenino , Fibrilina-1 , Fibrilina-2 , Fibrilinas , Fibrosis/metabolismo , Perfilación de la Expresión Génica , Glicoproteínas/genética , Humanos , Inmunohistoquímica , Péptidos y Proteínas de Señalización Intercelular , Metaloproteinasa 2 de la Matriz/genética , Proteínas de Microfilamentos/genética , Reacción en Cadena de la Polimerasa/métodos , Factores de Empalme de ARN , Análisis de Secuencia de ADN , Adulto JovenRESUMEN
ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Drosophila gene dMRP/CG6214 as an orthologue to the human multidrug-resistance associated proteins MRP1, MRP2, MRP3 and MRP6. To study evolutionarily conserved roles of MRPs we have initiated a characterization of dMRP. In situ hybridization and Northern analysis indicate that dMRP is expressed throughout development and appears to be head enriched in adults. Functional studies indicate that DMRP is capable of transporting a known MRP1 substrate and establishes DMRP as a high capacity ATP-dependent, vanadate-sensitive organic anion transporter.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Drosophila melanogaster/genética , Perfilación de la Expresión Génica , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética , Filogenia , Transportadoras de Casetes de Unión a ATP/metabolismo , Animales , Northern Blotting , Western Blotting , Análisis por Conglomerados , Cartilla de ADN , Proteínas de Drosophila , Humanos , Hibridación in Situ , Proteínas de la Membrana/metabolismo , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/metabolismo , Conformación Proteica , Especificidad de la EspecieRESUMEN
We have identified two elastin gene (ELN) mutations located in cis in two related families with supravalvular aortic stenosis (SVAS). These mutations included an in-frame duplication in exon 18 (1034-1057dup) and a single base substitution in exon 26 (1829G-->A) predicted to result in the amino acid substitution R610Q. Haplotype analysis in one of the families identified an individual with a recombination between exon 18 and 26 of the elastin gene. This individual was unaffected and carried the exon 18 insertion mutation but not 1829G-->A. Skin fibroblasts were established from this recombinant normal individual and from an affected individual carrying both of the mutations. Reverse transcription/polymerase chain reaction (RT-PCR) analysis indicated that the expression of the mutant allele was reduced to 12%-27% of the normal allele in the affected but not in the unaffected individual. RNA-blot hybridization and immunoprecipitation experiments revealed reduced steady-state elastin mRNA levels and tropoelastin synthesis in the affected individual. RT-PCR analysis of the mRNA rescued by cycloheximide treatment indicated that mutation 1829G-->A created a cryptic donor splice site within exon 26, resulting in the deletion of four nucleotides at the 3'-end of exon 26 and a frameshift in the mRNA. This frameshift mutation generated a premature termination codon in the domain encoded by exon 28, clearly resulting in nonsense-mediated decay (NMD) of this frameshift RNA product. Despite considerable variability in the molecular nature of mutations responsible for SVAS, the unifying mechanism appears to be the generation of null alleles by NMD leading to elastin haploinsufficiency.
Asunto(s)
Estenosis Aórtica Supravalvular/genética , Elastina/genética , Mutación Missense , Alelos , Secuencia de Aminoácidos , Secuencia de Bases , ADN , Cartilla de ADN , Exones , Femenino , Frecuencia de los Genes , Humanos , Masculino , Datos de Secuencia Molecular , Linaje , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
To better understand the pathogenetics of pseudoxanthoma elasticum (PXE), we performed a mutational analysis of ATP-binding cassette subfamily C member 6 (ABCC6) in 122 unrelated patients with PXE, the largest cohort of patients yet studied. Thirty-six mutations were characterized, and, among these, 28 were novel variants (for a total of 43 PXE mutations known to date). Twenty-one alleles were missense variants, six were small insertions or deletions, five were nonsense, two were alleles likely to result in aberrant mRNA splicing, and two were large deletions involving ABCC6. Although most mutations appeared to be unique variants, two disease-causing alleles occurred frequently in apparently unrelated individuals. R1141X was found in our patient cohort at a frequency of 18.8% and was preponderant in European patients. ABCC6del23-29 occurred at a frequency of 12.9% and was prevalent in patients from the United States. These results suggested that R1141X and ABCC6del23-29 might have been derived regionally from founder alleles. Putative disease-causing mutations were identified in approximately 64% of the 244 chromosomes studied, and 85.2% of the 122 patients were found to have at least one disease-causing allele. Our results suggest that a fraction of the undetected mutant alleles could be either genomic rearrangements or mutations occurring in noncoding regions of the ABCC6 gene. The distribution pattern of ABCC6 mutations revealed a cluster of disease-causing variants within exons encoding a large C-terminal cytoplasmic loop and in the C-terminal nucleotide-binding domain (NBD2). We discuss the potential structural and functional significance of this mutation pattern within the context of the complex relationship between the PXE phenotype and the function of ABCC6.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Mutación/genética , Seudoxantoma Elástico/genética , Transportadoras de Casetes de Unión a ATP/química , Alelos , Elementos Alu/genética , Secuencia de Bases , Estudios de Cohortes , Análisis Mutacional de ADN , Frecuencia de los Genes/genética , Haplotipos/genética , Humanos , Datos de Secuencia Molecular , Proteínas Asociadas a Resistencia a Múltiples Medicamentos , Mutación Missense/genética , Fenotipo , Seudogenes/genética , Eliminación de Secuencia/genéticaRESUMEN
High-resolution solid-state (13)C NMR spectra are presented for samples of alpha-elastin prepared from the aorta of normal and copper-deficient pigs. Chemical shifts of the various peaks indicate that both the normal and undercross-linked peptides have similar overall structures. However, (13)C T(1), (13)C T(1 rho), and (1)H T(1 rho) measurements indicate that the alpha-elastin peptides obtained from the abnormal elastic fibers samples exhibit altered mobilities, particularly in their side chains. Results from spectra taken with a range of contact times and from dipolar dephasing experiments are consistent with conclusions reached with the relaxation measurements. Namely, the loss of function associated with the undercross-linked sample is correlated to a small but measurable difference in relative mobility.
Asunto(s)
Elastina/química , Animales , Biopolímeros/química , Biopolímeros/aislamiento & purificación , Isótopos de Carbono , Cobre/deficiencia , Reactivos de Enlaces Cruzados , Elastina/aislamiento & purificación , Técnicas In Vitro , Espectroscopía de Resonancia Magnética , PorcinosRESUMEN
Previous histopathologic results have suggested that one mechanism whereby hyperglycemia (HG) leads to exaggerated ischemic damage involves fragmentation of DNA. DNA fragmentation in normoglycemia (NG) and HG rats subjected to 30 minutes of forebrain ischemia was studied by terminal deoxynucleotidyl transferase mediated DNA nick-labeling (TUNEL) staining, by pulse-field gel electrophoresis (PFGE), and by ligation-mediated polymerase chain reaction (LM-PCR). High molecular weight DNA fragments were detected by PFGE, whereas low molecular weight DNA fragments were detected using LM-PCR techniques. The LM-PCR procedure was performed on DNA from test samples with blunt (without Klenow polymerase) and 3'-recessed ends (with Klenow polymerase). In addition, cytochrome c release and caspase-3 activation were studied by immunocytochemistry. Results show that HG causes cytochrome c release, activates caspase-3, and exacerbates DNA fragments induced by ischemia. Thus, in HG rats, but not in control or NGs, TUNEL-stained cells were found in the cingulate cortex, neocortex, thalamus, and dorsolateral crest of the striatum, where neuronal death was observed by conventional histopathology, and where both cytosolic cytochrome c and active caspase-3 were detected by confocal microscopy. In the neocortex, both blunt-ended and stagger-ended fragments were detected in HG, but not in NG rats. Electron microscopy (EM) analysis was performed in the cingulate cortex, where numerous TUNEL-positive neurons were observed. Although DNA fragmentation was detected by TUNEL staining and electrophoresis techniques, EM analysis failed to indicate apoptotic cell death. It is concluded that HG triggers a cell death pathway and exacerbates DNA fragmentation induced by ischemia.
Asunto(s)
Fragmentación del ADN , Hiperglucemia/patología , Ataque Isquémico Transitorio/patología , Animales , Apoptosis , Caspasa 3 , Caspasas/metabolismo , Cuerpo Estriado/patología , Grupo Citocromo c/metabolismo , Giro Dentado/patología , Activación Enzimática , Hipocampo/patología , Hiperglucemia/fisiopatología , Etiquetado Corte-Fin in Situ , Ataque Isquémico Transitorio/fisiopatología , Masculino , Microscopía Electrónica , Neocórtex/patología , Neuronas/patología , Ratas , Ratas Wistar , Tálamo/patologíaRESUMEN
PXE International, a disease advocacy group, plays a central role as a catalyst in several research initiatives focused on the biology, epidemiology, and genetics of pseudoxanthoma elasticum (PXE). These initiatives accelerate research on PXE and provide a basis for several productive collaborative partnerships, including a particularly successful professional relationship between PXE International and the Laboratory of Matrix Pathobiology at the University of Hawaii. This partnership was critical in discovering the PXE gene and in beginning the elucidation of the pathobiology of this genetic disorder. Examination of some of the elements of this partnership may be useful for other lay advocacy/professional collaborations.
Asunto(s)
Conducta Cooperativa , Defensa del Paciente/tendencias , Seudoxantoma Elástico/genética , Investigación , Comunicación , Confidencialidad , Ética , Ética Clínica , Hawaii , Humanos , Consentimiento Informado , Cooperación Internacional , Mutación , Seudoxantoma Elástico/fisiopatología , Investigación/economía , Investigación/organización & administración , Investigación/tendencias , UniversidadesRESUMEN
Serial Analysis of Gene Expression (SAGE) is an innovative technique that offers the potential of cataloging both the identity and relative frequencies of mRNA transcripts in a given poly(A(+)) RNA preparation. Although it is a very effective approach for determining the expression of mRNA populations, there are significant biases in the observed results that are inherent in the experimental process. These are caused by sampling error, sequencing error, nonuniqueness, and nonrandomness of tag sequences. The quantitative information desired from SAGE experiments consists of estimates of the number of genes and the frequency distribution of transcript copy numbers. Of additional concern is the extent to which a given tag sequence can be assumed to be unique to its gene. The present study takes these mathematical biases into account and presents a basis for maximum likelihood estimation of gene number and transcript copy frequencies given a set of experimental results. These estimates of the true state of genomic expression are markedly different from those based directly on the observations from the underlying experiments. It also is shown that while in many cases it is probable that a given tag sequence is unique within the genome, in larger genomes this cannot be safely assumed.
Asunto(s)
Expresión Génica , ARN Mensajero/biosíntesis , Análisis de Secuencia de ADN/métodos , Simulación por Computador , Sondas de ADN , Dosificación de Gen , Proyectos de Investigación , Análisis de Secuencia de ADN/estadística & datos numéricos , Lugares Marcados de Secuencia , Procesos EstocásticosRESUMEN
We have used single-strand conformation and heteroduplex analyses of genomic amplimers to identify point mutations within the elastin gene (ELN) in patients with non-syndromic supravalvular aortic stenosis (SVAS) from a total of eight unrelated families. Six novel point mutations were identified. We have collected detailed clinical information on mutation carriers and demonstrated significant non-penetrance in some of the families. Together with the new mutations described here, 14 point mutations have been reported in SVAS patients, and 10 of these result in premature stop codons (PTCs). We have analyzed the expression of ELN alleles in skin fibroblasts from one SVAS patient and shown that PTC mutations indeed result in selective elimination of mutant transcripts. Inhibition of the nonsense-mediated decay mechanism by cycloheximide resulted in the stabilization of mutant elastin mRNA. Allelic inactivation by the ELN mutation in this patient led to an overall decrease of the steady state levels of elastin mRNA. Finally, we have demonstrated reduced synthesis and secretion of tropoelastin by skin fibroblasts from the same SVAS patient. We conclude that PTC mutations in ELN result in nonsense-mediated decay of mutant mRNA in this patient. Given the predominance of PTC mutations in SVAS, we suggest that functional haploinsufficiency may be a pathomechanism underlying most cases of non-syndromic SVAS.
Asunto(s)
Estenosis de la Válvula Aórtica/genética , Elastina/genética , Mutación Puntual/genética , Adolescente , Adulto , Anciano , Alelos , Estenosis de la Válvula Aórtica/diagnóstico , Células Cultivadas , Niño , Preescolar , Cicloheximida/farmacología , Elastina/biosíntesis , Femenino , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fibroblastos/metabolismo , Frecuencia de los Genes , Silenciador del Gen , Tamización de Portadores Genéticos , Pruebas Genéticas , Humanos , Lactante , Masculino , Persona de Mediana Edad , Penetrancia , Polimorfismo Genético , Procesamiento Postranscripcional del ARN/genética , ARN Mensajero/metabolismoRESUMEN
Pseudoxanthoma elasticum (PXE) is a heritable disorder characterized by calcification of elastic fibres in skin, arteries and retina that results in dermal lesions with associated laxity and loss of elasticity, arterial insufficiency and retinal haemorrhages leading to macular degeneration. PXE is usually found as a sporadic disorder, but examples of both autosomal recessive and autosomal dominant forms of PXE have been observed. Partial manifestations of the PXE phenotype have also been described in presumed carriers in PXE families. Linkage of both dominant and recessive forms of PXE to a 5-cM domain on chromosome 16p13.1 has been reported (refs 8,9). We have refined this locus to an 820-kb region containing 6 candidate genes. Here we report the exclusion of five of these genes and the identification of the first mutations responsible for the development of PXE in a gene encoding a protein associated with multidrug resistance (ABCC6).
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Mutación/genética , Seudoxantoma Elástico/genética , Transportadoras de Casetes de Unión a ATP/química , Secuencia de Aminoácidos , Secuencia de Bases , Cromosomas Humanos Par 16/genética , Estudios de Cohortes , Consanguinidad , Análisis Mutacional de ADN , Exones/genética , Femenino , Fibroblastos/metabolismo , Ligamiento Genético/genética , Genotipo , Humanos , Masculino , Repeticiones de Microsatélite/genética , Proteínas Asociadas a Resistencia a Múltiples Medicamentos , Linaje , Fenotipo , Polimorfismo de Nucleótido Simple/genética , Conformación Proteica , Seudoxantoma Elástico/patología , ARN Mensajero/genética , ARN Mensajero/metabolismoAsunto(s)
Enfermedades del Tejido Conjuntivo/metabolismo , Tejido Elástico/metabolismo , Transporte Biológico , Enfermedades del Tejido Conjuntivo/enzimología , Enfermedades del Tejido Conjuntivo/genética , Enfermedades del Tejido Conjuntivo/patología , Tejido Elástico/enzimología , Tejido Elástico/patología , Tejido Elástico/fisiopatología , Gangliosidosis GM1/enzimología , Gangliosidosis GM1/genética , Gangliosidosis GM1/metabolismo , Gangliosidosis GM1/patología , Humanos , Síndrome de Marfan/genética , Síndrome de Marfan/metabolismo , Síndrome de Marfan/patología , Síndrome del Pelo Ensortijado/genética , Síndrome del Pelo Ensortijado/metabolismo , Síndrome del Pelo Ensortijado/patología , Mucopolisacaridosis I/genética , Mucopolisacaridosis I/metabolismo , Mucopolisacaridosis I/patología , Mucopolisacaridosis IV/enzimología , Mucopolisacaridosis IV/genética , Mucopolisacaridosis IV/metabolismo , Mucopolisacaridosis IV/patología , Síndrome , Síndrome de Williams/genética , Síndrome de Williams/metabolismo , Síndrome de Williams/patología , beta-Galactosidasa/deficiencia , beta-Galactosidasa/genética , beta-Galactosidasa/metabolismoRESUMEN
Williams syndrome (WS) is a complex developmental disorder with multisystem involvement known to be the result of a microdeletion in the q11.23 region of chromosome 7. This deletion involves several genes, including the elastin gene. Although elastic fibers are important constituents of skin, little is known about the skin phenotype in WS patients. We have therefore studied the skin of four WS patients in which we've shown the deletion of one copy of the elastin gene. Physical examination and indirect immunofluorescent microscopy of elastin did not detect any major phenotypic or morphologic changes in the skin. We were able, however, to show subtle textural changes in skin and, by electron microscopy, that the amorphous component of elastic fibers in WS patients was consistently reduced when compared to normal controls. These findings indicate that deletion of one copy of the elastin gene results in reduced deposition of elastin in dermal elastic fibers, an altered elastic fiber ultrastructure, and a subclinical dermal phenotype in the children and young adult patients analyzed in this study.
Asunto(s)
Tejido Elástico/patología , Elastina/genética , Eliminación de Gen , Piel/patología , Síndrome de Williams/genética , Adulto , Niño , Preescolar , Tejido Elástico/ultraestructura , Femenino , Humanos , Masculino , Fenotipo , Síndrome de Williams/diagnósticoRESUMEN
We have performed linkage analysis on 21 families with pseudoxanthoma elasticum (PXE) using 10 polymorphic markers located on chromosome 16p13.1. The gene responsible for the PXE phenotype was localized to an 8-cM region of 16p13.1 between markers D16S500 and D16S3041 with a maximum lod score of 8.1 at a recombination fraction of 0.04 for marker D16S3017. The lack of any locus heterogeneity suggests that the major predisposing allele for the PXE phenotype is located in this region. Haplotype studies of a total of 36 PXE families identified several recombinations that further confined the PXE gene to a region (< 1 cM) between markers D16S3060 and D16S79. This PXE locus was identified within a single YAC clone and several overlapping BAC recombinants. From sequence analysis of these BAC recombinants, it is clear that the distance between markers D16S3060 and D16S79 is about 820 kb and contains a total of nine genes including three pseudogenes. We predict that mutations in one of the expressed genes in the locus will be responsible for the PXE phenotype in these families.
Asunto(s)
Cromosomas Humanos Par 16/genética , Seudoxantoma Elástico/genética , Alelos , Mapeo Cromosómico , Cromosomas Artificiales de Levadura , Intercambio Genético , Femenino , Genes , Haplotipos/genética , Humanos , Escala de Lod , Masculino , SeudogenesRESUMEN
Two known glycosides and a novel trisaccharide fatty acid ester were isolated from the n-butanol-soluble fraction of the fruits of Morinda citrifolia (noni). Structure determination was carried out by spectral techniques such as MS, IR, NMR, and 2D-NMR. The novel trisaccharide fatty acid ester was elucidated as 2, 6-di-O-(beta-D-glucopyranosyl)-1-O-octanoyl-beta-D-glucopyranose. The known compounds were identified as rutin and asperulosidic acid.
Asunto(s)
Citrus/química , Ácidos Grasos/aislamiento & purificación , Trisacáridos/aislamiento & purificación , Monoterpenos Ciclopentánicos , Ésteres , Ácidos Grasos/química , Glucósidos/análisis , Glucósidos/química , Humanos , Espectroscopía de Resonancia Magnética , Piranos/análisis , Piranos/química , Rutina/análisis , Rutina/química , Espectrometría de Masa Bombardeada por Átomos Veloces , Espectrofotometría Infrarroja , Trisacáridos/químicaRESUMEN
Previous evidence has demonstrated the absence of exons 34 and 35 within the 3' end of the human tropoelastin (ELN) gene. These exons encode conserved polypeptide domains within tropoelastin and are found in the ELN gene in vertebrate species ranging from chickens to rats to cows. We have analyzed the ELN gene in a variety of primate species to determine whether the absence of exons 34 and 35 in humans either is due to allelic variation within the human population or is a general characteristic of the Primates order. An analysis of the 3' end of the ELN gene in several nonhuman primates and in 546 chromosomes from humans of varying ethnic background demonstrated a sequential loss of exons 34 and 35 during primate evolution. The loss of exon 35 occurred at least 35-45 million years ago, when Catarrhines diverged from Platyrrhines (New World monkeys). Exon 34 loss, in contrast, occurred only about 6-8 million years ago, when Homo separated from the common ancestor shared with chimpanzees and gorillas. Loss of both exons was probably facilitated by Alu-mediated recombination events and possibly conferred a functional evolutionary advantage in elastic tissue.
Asunto(s)
Evolución Molecular , Primates/genética , Tropoelastina/genética , Alelos , Animales , Secuencia de Bases , Bovinos , ADN/genética , Cartilla de ADN/genética , Exones , Variación Genética , Humanos , Datos de Secuencia Molecular , Papio/genética , Reacción en Cadena de la Polimerasa , Ratas , Eliminación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Factores de TiempoRESUMEN
We have screened the elastin gene for mutations responsible for supravalvular aortic stenosis (SVAS) in two large, independently collected families with isolated (nonsyndromic) SVAS. By single-strand conformation polymorphism and heteroduplex analysis, we have identified a C to G transversion within the acceptor splice site of exon 16 in SVAS patients from both families. This mutation segregates in both families with high penetrance of SVAS, and all affected individuals carry the mutation. Haplotype analysis by using closely linked polymorphisms, including a previously unreported BfaI restriction fragment length polymorphism within the 3'-UTR of the elastin gene, indicates that the mutations found in the two apparently non-overlapping kindreds are identical by descent. To study the effect of the mutation on the expression of the mutant allele, we have established a primary skin fibroblast culture from one of the affected individuals. Reverse transcription/polymerase chain reaction analysis of elastin mRNA species indicates that the mutation results in two abnormal elastin mRNA species. One mutant elastin mRNA is generated by the activation of a cryptic splice site that lies within intron 15 and that adds 44 bp of intronic sequence to the sequence encoded by exon 16. This insertion creates a frame shift that results in a 59-amino-acid-long abnormal protein sequence and leads to a termination codon in the mRNA sequence encoded by exon 17. The smaller abnormal mRNA species arises as a consequence of the skipping of exon 16. This study demonstrates, for the first time, the expression of mutant alleles of the elastin gene in patients with isolated SVAS.
Asunto(s)
Empalme Alternativo , Estenosis de la Válvula Aórtica/genética , Elastina/genética , Mutación , Sitios de Unión , Femenino , Regulación de la Expresión Génica , Humanos , Masculino , Linaje , Empalme del ARNRESUMEN
We have reported in this paper the complete cDNA sequence, gene structure, and tissue-specific expression of LOXL2, a new amine oxidase and a member of an emerging family of human lysyl oxidases. The predicted amino acid sequence, from several overlapping cDNA clones isolated from placenta and spleen cDNA libraries, shared extensive sequence homology with the conserved copper-binding and catalytic domains of both lysyl oxidase (LOX) and the lysyl oxidase-like (LOXL) protein. These conserved domains are encoded by five consecutive exons within the LOX, LOXL, and LOXL2 genes that also maintained exon-intron structure conservation. In contrast, six exons encoding the amino-terminal domains diverged both in sequence and structure. Exon 1 of the LOXL2 gene does not encode a signal sequence that is present in LOX and LOXL, suggesting a different processing and intracellular localization for this new protein. Expression of the LOXL2 gene was detected in almost all tissues with the highest steady state mRNA levels in the reproductive tissues, placenta, uterus and prostate. In situ hybridization identified placental syncytial and cytotrophoblasts responsible for the synthesis of LOXL2 mRNA and demonstrated a spatial and temporal expression pattern unique to the LOXL2 gene.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Expresión Génica , Placenta/enzimología , Secuencia de Bases , Clonación Molecular , ADN Complementario , Exones , Humanos , Intrones , Datos de Secuencia Molecular , Regiones Promotoras Genéticas , Transcripción GenéticaRESUMEN
The elasticity of tissues subjected to repeated deformation is provided by the presence of elastic fibers in the extracellular matrix (ECM). The most abundant component of elastic fibers is elastin, whose soluble precursor is tropoelastin. To establish the role elastin plays in the bladder, this study describes the biosynthetic, histologic, and physiologic consequences of expression of an isoform of rat tropoelastin in transgenic mouse bladder. The polymerase chain reaction (PCR) was used to determine expression of a rat tropoelastin minigene in transgenic mice. Histochemical methods were used to demonstrate changes in elastic fibers in frozen sections of bladder. Cystometric analysis was carried out in transgenic and non-transgenic mice, prior to and after 3 weeks of partial outlet obstruction. The PCR assay demonstrated that bladder tissue of transgenic mice expressed rat tropoelastin mRNA, whereas non-transgenes did not. Increased deposition of elastic fibers was demonstrated with the Verhoeff-van Gieson stain. Bladders of transgenic animals were more compliant than bladders of their non-transgenic littermates. Partial outlet obstruction resulted in increased bladder volume and more compliant bladders in non-transgenic mice. In contrast, the bladder volume and compliance in transgenes was almost unchanged by obstruction. This study demonstrates that normal elastic fiber assembly is prerequisite for the compliant properties of the bladder wall. Moreover, the response of the bladder to obstruction is critically influenced by elastin synthesis.
Asunto(s)
Tropoelastina/fisiología , Obstrucción del Cuello de la Vejiga Urinaria/fisiopatología , Vejiga Urinaria/fisiología , Animales , Adaptabilidad , Cartilla de ADN/química , Femenino , Expresión Génica , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Músculo Liso/citología , Músculo Liso/metabolismo , Mutación , Fenotipo , ARN Mensajero/biosíntesis , Ratas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Vejiga Urinaria/citología , Obstrucción del Cuello de la Vejiga Urinaria/patologíaRESUMEN
We have isolated a mouse lysyl oxidase-like (LOXL) cDNA from a mouse embryo cDNA library and used this cDNA to measure changes in steady state levels of LOXL mRNA during the development of carbon tetrachloride-induced liver fibrosis in adult mice. These results revealed the coincident appearance of increased steady state levels of LOXL mRNA and type III procollagen mRNA early in the development of liver fibrosis. In contrast, steady state levels of lysyl oxidase mRNA increased throughout the onset of hepatic fibrosis and appeared in parallel with the increased steady state levels of pro-alphaI (I) collagen mRNA. These findings suggest that the LOXL protein (possibly an isoform of lysyl oxidase) is involved in the development of lysine-derived cross-links in collagenous substrates. Moreover, the substrate specificity of the LOXL protein may be different to that of lysyl oxidase and this difference may be collagen-type specific.