RESUMO
We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4 +, CEM-SS cells infected with HIV/1, strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of approximately 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity.
Assuntos
Fármacos Anti-HIV/farmacologia , Linfócitos T CD4-Positivos/virologia , Avaliação Pré-Clínica de Medicamentos/métodos , HIV-1/efeitos dos fármacos , Membranas Artificiais , Resinas Acrílicas , Animais , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/transplante , Sobrevivência Celular , Efeito Citopatogênico Viral , Proteína do Núcleo p24 do HIV/análise , Transcriptase Reversa do HIV/metabolismo , HIV-1/crescimento & desenvolvimento , HIV-1/metabolismo , Humanos , Camundongos , Permeabilidade , Polímeros , Cloreto de Polivinila , Próteses e Implantes , Zidovudina/farmacologiaRESUMO
The disruption of the viral coat of human immunodeficiency virus by Triton X-100, a nonionic detergent, is a time-dependent process which requires incubation times of 30 min or longer. Conditions for the production of a noninfectious sample from a viral pellet that can be used to measure reverse transcriptase activity were determined.