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1.
FASEB J ; 37(10): e23173, 2023 10.
Article in English | MEDLINE | ID: mdl-37665572

ABSTRACT

The poor prognosis of immunotherapy in patients with colorectal cancer (CRC) necessitates a comprehensive understanding of the immunosuppressive mechanisms within tumor microenvironment (TME). Undoubtedly, the anti-tumor immune cells play an indispensable role in immune tolerance. Therefore, it is imperative to investigate novel immune-related factors that have the capacity to enhance anti-tumor immunity. Here, we employed bioinformatic analysis using R and Cytoscape to identify the hub gene chemokine (C-X-C motif) ligand 8 (CXCL8), which is overexpressed in CRC, in the malignant progression of CRC. However, its specific role of CXCL8 in CRC immunity remains to be elucidated. For this purpose, we evaluated how tumor-derived CXCL8 promotes M2 macrophage infiltration by in vivo and in vitro, which can be triggered by IL-1ß within TME. Mechanistically, CXCL8-induced polarization of M2 macrophages depends on the activation of the STAT3 signaling. Finally, immunohistochemistry and multiplexed immunohistochemistry analysis identified that CXCL8 not only enhances PD-L1+ M2 macrophage infiltration but also attenuates the recruitment of PD-1+ CD8+ T cells in murine CRC models. Together, these findings emphasize the critical role for CXCL8 in promoting M2 macrophage polarization and inhibiting CD8+ T cell infiltration, thereby links CXCL8 to the emergency of immunosuppressive microenvironment facilitating tumor evasion. Overall, these findings may provide novel strategy for CRC immunotherapy.


Subject(s)
CD8-Positive T-Lymphocytes , Colorectal Neoplasms , Interleukin-8 , Animals , Humans , Mice , Computational Biology , Immunosuppressive Agents , Macrophages , Tumor Microenvironment , Interleukin-8/genetics
2.
Opt Express ; 31(17): 27407-27419, 2023 Aug 14.
Article in English | MEDLINE | ID: mdl-37710817

ABSTRACT

In this paper, various hollow structured optical fields are generated by skillfully adjusting the number and positions of multiple off-axis vortices loaded in a Gaussian beam. The focal-field characteristics of the generated hollow structured optical fields after passing through an ordinary lens are studied based on the scalar diffraction theory. Firstly, a variety of hollow structured optical fields are theoretically simulated by adjusting the number and positions of multiple off-axis vortices loaded in the Gaussian beam. The focal-field characteristics of the hollow structured optical fields after passing through a lens are theoretically analyzed. On this basis, the experiments are implemented in the built optical system for multi-off-axis vortex beam focusing through an ordinary lens. In the experiments, various hollow structured optical fields are detected in CCD which are consistent with the theoretical results. The manipulations of size and rotation direction of the hollow structured optical fields are realized. We believe that this study will contribute to extending the potential applications of off-axis vortex beams in fields such as optical field shaping, optical manipulation and laser processing.

3.
Chemistry ; 27(52): 13123-13127, 2021 Sep 15.
Article in English | MEDLINE | ID: mdl-34369008

ABSTRACT

An effective and condition-controlled way to synthesize with high selectivity a variety of functionalized indoles with potent biological properties has been developed. Notably, 2,4-dialkynyl indole products were obtained by direct double C-H bond alkynylation, whereas alkynyl at the C4 position could convert to carbonyl to generate 2-alkynyl-3,4-diacetyl indoles fast and effectively. Additionally, a one-pot relay catalytic reaction led to 2,5-di-alkynyl-3,4-diacetyl indoles when using a carbonyl group as the directing group and by controlling the type and quantity of additives. A possible mechanism was proposed based on many studies including deuterium-exchange experiments, the necessary conditions of product conversion, and the effect of water on the reaction.


Subject(s)
Indoles , Catalysis
4.
Fish Shellfish Immunol ; 73: 84-91, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29191796

ABSTRACT

The yellow catfish, Pelteobagrus fulvidraco, has been recognized as an important freshwater aquaculture species in Eastern and Southeast Asia. To gain a better understanding of the immune response in P. fulvidraco, we analyzed its transcriptome following stimulation with lipopolysaccharide (LPS). Phosphate buffer saline (PBS) was used as control. Following assembly and annotation, 72,152 unigenes with an average length of 1090 bp were identified. A total of 370 differentially expressed genes (DEGs) in the P. fulvidraco were observed at 12 h post LPS treatment, including 197 up-regulated genes and 173 down-regulated genes. Clusters of Orthologous Groups of proteins (KOG/COG) annotation demonstrated that a total of 18,819 unigenes classified into 26 categories. Gene ontology (GO) analysis revealed 20 biological process subcategories, 7 cellular component subcategories and 20 molecular function subcategories. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified immune responses pathways. Quantitative reverse transcription polymerase chain reaction measured the expression of 18 genes involved in the immune response. CXCL2-like chemokine (CXCL2), goose-type lysozyme (LYZ G), and cathepsin K (CTSK) were significantly up-regulated. This study enriches the P. fulvidraco transcriptome database and provides insight into the immune response of P. fulvidraco against infection.


Subject(s)
Catfishes/genetics , Catfishes/immunology , Fish Proteins/genetics , Gene Expression Regulation/immunology , Immunity, Innate/genetics , Lipopolysaccharides/pharmacology , Transcriptome , Animals , Fish Proteins/metabolism , Liver/drug effects , Liver/immunology , Reverse Transcriptase Polymerase Chain Reaction/veterinary
5.
Genomics ; 2017 Oct 03.
Article in English | MEDLINE | ID: mdl-28982639

ABSTRACT

The mitochondrial genome (mitogenome) provides important information for phylogenetic analysis and understanding evolutionary origins. Herein, we sequenced, annotated, and characterised the mitogenome of the crab Helice wuana to better understand its molecular evolution and phylogeny. The 16,359bp mitogenome includes 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and one control region. The genome composition is highly A+T biased 68.42%, and exhibits a negative AT-skew (-0.036) and GC-skew (-0.269) among Brachyura crabs. Gene rearrangements were detected, as was tandem duplication followed by random loss, which explains the translocation of mitochondrial genes. Phylogenetic analysis showed that H. wuana and H. tientsinensis clustered on one branch with high nodal support values. These results confirm that the placement of H. wuana within the Varunidae family of Thoracotrematan crabs. This study will provided a better understanding for gene rearrangements and crab evolution in the further.

6.
Biochem Biophys Res Commun ; 489(2): 149-154, 2017 07 22.
Article in English | MEDLINE | ID: mdl-28546004

ABSTRACT

Gene arrangements in the mitochondrial genomes (mitogenomes) of insects are conserved across the major lineages, but can be rearranged within derived groups and may provide valuable phylogenetic characters. In this study, we sequenced the entire mitogenome of Parasa consocia, a moth of the family Limacodidae (Lepidoptera: Zygaenoidea). Compared with other lepidopterans and ancestral insects, the P. consocia mitogenome features a transfer RNA gene arrangement novel among lepidopterans between the ND3 and ND5 genes: RANSEF (the underline signifies an inverted gene), which differs from the ARNSEF arrangement of ancestral insects. This rearrangement can be explained by the tandem duplication-random loss model. We inferred a phylogenetic hypothesis for the lepidopteran superfamily based on mitochondrial amino-acid sequences using the Bayesian-inference and maximum-likelihood methods. Our results showed that P. consocia belongs to the Zygaenoidea superfamily and supported the following phylogenetic relationship: Yponomeutoidea + (Tortricoidea + Zygaenoidea + (Papilionoidea + (Pyraloidea + (Noctuoidea + (Geometroidea + Bombycoidea)))))). Comparative analyses indicated that mitogenomes are a useful phylogenetic tool at the subfamily level within the order Lepidoptera. Our findings also suggest that mitogenomes are likely to represent a valuable tool for systematics in other groups of lepidopterans.


Subject(s)
Gene Order/genetics , Genome, Mitochondrial/genetics , RNA, Transfer/genetics , Animals , Moths , Phylogeny
7.
Fish Shellfish Immunol ; 60: 247-254, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27815205

ABSTRACT

Hepcidin is a small, cysteine-rich antimicrobial peptide with a highly conserved ß-sheet structure that plays a vital role in innate host immunity against pathogenic organisms. In this study, a hepcidin gene was identified in Pelteobagrus fulvidraco, an economically important freshwater fish in China. The gene is named PfHep. The complete PfHep cDNA was 723 bp, including a 5'-untranslated region (UTR) of 102 bp, a 3'-UTR of 339 bp and an open reading frame of 282 bp encoding a polypeptide of 93 amino acids, which includes a predicted signal peptide and the Hepcidin domain. The predicted mature, cationic PfHep protein has a typical hepcidin RX (K/R)R motif and eight conserved cysteine residues. The deduced PfHep protein sequence has 70%, 54% and 39% percent identity with hepcidins from Ictalurus punctatus, Danio rerio, and Homo sapiens, respectively. The predicted tertiary structure of PfHep is very similar to that of hepcidin in other animals. Phylogenetic analysis revealed that PfHep is closely related to the hepcidins of I. punctatus and I. furcatus. Real-time quantitative reverse transcription-PCR showed that the PfHep gene was expressed most in liver of healthy P. fulvidraco, and expressed to some extent in all the tissues tested. After challenge with lipopolysaccharide and polyriboinosinic:polyribocytidylic acid (poly I:C), respectively, the expression levels of PfHep were markedly upregulated in liver, spleen, head kidney and blood at different time points. Together these results imply that PfHep may be an important component of the innate immune system and be involved in immune defense against invading pathogens.


Subject(s)
Catfishes/genetics , Fish Proteins/genetics , Gene Expression Regulation , Hepcidins/genetics , Immunity, Innate , Amino Acid Sequence , Animals , Base Sequence , Catfishes/immunology , Catfishes/metabolism , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/metabolism , Fish Proteins/chemistry , Fish Proteins/metabolism , Gene Expression Profiling , Hepcidins/chemistry , Hepcidins/metabolism , Lipopolysaccharides/pharmacology , Phylogeny , Poly I-C/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Real-Time Polymerase Chain Reaction/veterinary , Sequence Alignment/veterinary
8.
Fish Shellfish Immunol ; 68: 395-403, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28732769

ABSTRACT

Yellow catfish (Pelteobagrus fulvidraco) is one of the most important economic freshwater species in China. However, infection by bacterial pathogenic diseases has caused high mortality and great economic loss in aquaculture. It is necessary for disease control to know more about the P. fulvidraco immune system and its related genes in response to bacterial or viral infections. In this study, the transcriptomic profiles of liver from P. fulvidraco stimulated by polyriboinosinic polyribocytidylic acid (poly I:C) was analyzed using high-throughput sequencing method. After assembly and annotation, total 67,447 unigenes were acquired, with an average length of 1091 bp. Under the infection of poly I:C, 522 differentially expressed genes (DEGs) were identified, including 307 up-regulated genes and 215 down-regulated genes. To further investigate the immune-related DEGs, Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were performed. The result of GO enrichment indicated gene response to external stimulus, regulation of response to stimulus, cellular response to stimulus, immune response and immune system progress. Significant KEGG enrichment analysis identified major immune related pathways. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that 13 immune response genes were identified to be up-regulated after 12 h of poly I:C stimulation compared to controls. Taken together, the results of our study are beneficial for better understanding of the immune system and defense mechanisms of yellow catfish in response to poly I:C infection.


Subject(s)
Catfishes/genetics , Catfishes/immunology , Fish Proteins/genetics , Liver/drug effects , Poly I-C/pharmacology , Transcriptome , Adjuvants, Immunologic/pharmacology , Animals , Catfishes/metabolism , Fish Proteins/metabolism , Gene Expression Profiling/veterinary , High-Throughput Nucleotide Sequencing/veterinary
9.
Fish Shellfish Immunol ; 63: 297-303, 2017 Apr.
Article in English | MEDLINE | ID: mdl-28232280

ABSTRACT

Ferritin plays important roles in iron storage, detoxification, and immune response. Here, a ferritin gene (PcFer) was identified in Procambarus clarkii, an economically important freshwater crayfish. Full-length PcFer cDNA was 1022-bp, including a 135-bp 5'-untranslated region (UTR) with a typical iron responsive element, a 374-bp 3'-UTR, and a 513-bp open reading frame encoding a polypeptide of 170 amino acids which contained the Ferritin domain. PcFer has ion binding sites, a ferrihydrite nucleation center, and an iron ion channel. PcFer is phylogenetically closely-related to Pacifastacus leniusculus and Eriocheir sinensis ferritins. Real-time quantitative reverse-transcription PCR analysis showed that PcFer was expressed in all tested P. clarkii tissues, and expressed most in hepatopancreas. After challenge with various heavy metals and lipopolysaccharide, respectively, the hepatopancreatic expression levels of PcFer were markedly upregulated. These results suggest that expression of PcFer might be involved in immune defense and protection of P. clarkii against heavy metal stress.


Subject(s)
Arthropod Proteins/genetics , Astacoidea/genetics , Ferritins/genetics , Lipopolysaccharides/pharmacology , Metals, Heavy/toxicity , Water Pollutants, Chemical/toxicity , Amino Acid Sequence , Animals , Arthropod Proteins/chemistry , Arthropod Proteins/metabolism , Astacoidea/immunology , Base Sequence , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/metabolism , Ferritins/chemistry , Ferritins/metabolism , Immunity, Innate , Phylogeny , Polymerase Chain Reaction/veterinary , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Alignment , Tissue Distribution
10.
Genome ; 59(1): 37-49, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26701149

ABSTRACT

The mitochondrial (mt) genome can provide important information for the understanding of phylogenetic relationships. The complete mt genome of Plodia interpunctella (Lepidoptera: Pyralidae) has been sequenced. The circular genome is 15 287 bp in size, encoding 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The AT skew of this mt genome is slightly negative, and the nucleotide composition is biased toward A+T nucleotides (80.15%). All PCGs start with the typical ATN (ATA, ATC, ATG, and ATT) codons, except for the cox1 gene which may start with the CGA codon. Four of the 13 PCGs harbor the incomplete termination codon T or TA. All the tRNA genes are folded into the typical clover-leaf structure of mitochondrial tRNA, except for trnS1 (AGN) in which the DHU arm fails to form a stable stem-loop structure. The overlapping sequences are 35 bp in total and are found in seven different locations. A total of 240 bp of intergenic spacers are scattered in 16 regions. The control region of the mt genome is 327 bp in length and consisted of several features common to the sequenced lepidopteran insects. Phylogenetic analysis based on 13 PCGs using the Maximum Likelihood method shows that the placement of P. interpunctella was within the Pyralidae.


Subject(s)
Genes, Insect , Genome, Mitochondrial , Lepidoptera/genetics , Animals , Base Sequence , Codon , DNA, Intergenic , Genes, Overlapping , Locus Control Region , Phylogeny , RNA, Ribosomal/genetics , RNA, Transfer
11.
Fish Shellfish Immunol ; 58: 423-428, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27645907

ABSTRACT

Lysozymes, innate immunity molecules, play a vital role in immune response to pathogens. The yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. The aim of this study was to quantify expression of the P. fulvidraco LysG gene (a g-type lysozyme) in response to pathogen-associated molecular patterns (PAMP) challenge. First, the P. fulvidraco LysG gene (PfLysG) was cloned and characterized. The full-length cDNA of PfLysG is 1323 bp, including a 5'-untranslated region (UTR) of 131 bp, a 3'-UTR of 634 bp, and an open reading frame of 558 bp encoding a polypeptide of 185 amino acids, which contains a transglycosylase SLT domain (Pfam01464). The predicted molecular weight of the protein is 20.52 kDa with a pI of 9.08. Two catalytic residues and seven N-acetyl-D-glucosamine binding sites are conserved in the sequence and there is no predicted signal peptide. The deduced PfLysG protein sequence has 84%, 76% and 69% percent identity with the LysGs from Ictalurus furcatus, Danio rerio, and Salmo salar, respectively. The predicted tertiary structure of PfLysG is very similar to that from other animals. Phylogenetic analysis showed that PfLysG is closely related to those from Teleostei. Real-time quantitative reverse transcription-PCR (qPCR) analysis showed that PfLysG was expressed in all examined tissues and most highly expressed in head kidney, spleen, and intestine. After simulated pathogen challenge with lipopolysaccharide and polyriboinosinic polyribocytidylic acid, respectively, the mRNA expression of PfLysG was upregulated significantly at different time points. The results suggest that the identified g-type lysozyme of P. fulvidraco is involved in innate immune responses.


Subject(s)
Catfishes/genetics , Fish Proteins/genetics , Gene Expression Regulation, Enzymologic , Muramidase/genetics , Amino Acid Sequence , Animals , Base Sequence , Catfishes/immunology , Catfishes/metabolism , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/metabolism , Enzyme Activation/drug effects , Fish Proteins/chemistry , Fish Proteins/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Lipopolysaccharides/pharmacology , Muramidase/chemistry , Muramidase/metabolism , Pathogen-Associated Molecular Pattern Molecules/pharmacology , Phylogeny , Poly I-C/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Alignment/veterinary
12.
Fish Shellfish Immunol ; 49: 16-23, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26706223

ABSTRACT

The adenine nucleotide translocases (ANTs) play a vital role in energy metabolism via ADP/ATP exchange in eukaryotic cells. Apostichopus japonicus (Echinodermata: Holothuroidea) is an important economic species in China. Here, a cDNA representing an ANT gene of A. japonicus was isolated and characterized from respiratory tree and named AjANT. The full-length AjANT cDNA is 1924 bp, including a 5'-untranslated region (UTR) of 38 bp, 3'-UTR of 980 bp and an open reading frame (ORF) of 906 bp encoding a polypeptide of 301 amino acids. The protein contains three homologous repeat Mito_carr domains (Pfam00153). The deduced AjANT protein sequence has 49-81% in comparison to ANT proteins from other individuals. The predicted tertiary structure of AjANT protein is highly similar to animal ANT proteins. Phylogenetic analysis showed that the AjANT is closely related to Holothuroidea ANT genes. Real-time quantitative reverse transcription-PCR (qPCR) analysis showed that AjANT expression is higher in the respiratory tree than in other examined tissues. After thermal stress or LPS challenge, expression of AjANT was significantly fluctuant compared to the control. These results suggested that changes in the expression of ANT gene might be involved in immune defense and in protecting A. japonicus against thermal stress.


Subject(s)
Gene Expression Regulation/drug effects , Hot Temperature , Immunity, Innate/drug effects , Lipopolysaccharides/pharmacology , Mitochondrial ADP, ATP Translocases/genetics , Stichopus/genetics , Stichopus/immunology , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA, Complementary/genetics , DNA, Complementary/metabolism , Gene Expression Profiling , Mitochondrial ADP, ATP Translocases/chemistry , Mitochondrial ADP, ATP Translocases/metabolism , Molecular Sequence Data , Phylogeny , Protein Structure, Tertiary , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Alignment , Stichopus/metabolism , Tissue Distribution
13.
Fish Shellfish Immunol ; 56: 278-285, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27368543

ABSTRACT

The yellow catfish, Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. However, genomic research and resources on this species are largely unavailable and still in infancy. In the present study, we constructed a cDNA library following poly I:C injection to screen for immune response genes in the spleens of P. fulvidraco using suppression subtractive hybridization (SSH). A total of 420 putative expressed sequence tag (EST) clones were identified at 24 h post-injection, which contain 103 genes consisting of 25 immune response genes, 12 cytoskeleton genes, 7 cell cycle and apoptosis genes, 7 respiration and energy metabolism genes, 7 transport genes, 26 metabolism genes, 10 stress response genes, 9 translational regulation genes, and 71 unknown genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) results revealed that a set of randomly selected immune response genes were identified to be up-regulated after 24 h of poly I:C stimulation compared to controls. Our study provides an annotation of immune genes in detail and insight into fish immunity.


Subject(s)
Catfishes/genetics , Fish Proteins/genetics , Immunity, Innate , Poly I-C/pharmacology , Animals , Catfishes/immunology , Catfishes/metabolism , Fish Proteins/metabolism , Gene Library , Real-Time Polymerase Chain Reaction/veterinary , Sequence Analysis, DNA/veterinary , Spleen/immunology , Spleen/metabolism , Subtractive Hybridization Techniques/veterinary , Up-Regulation
14.
Fish Shellfish Immunol ; 56: 248-254, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27235365

ABSTRACT

Fish are considered an excellent model for studies in comparative immunology as they are a representative population of lower vertebrates linked to invertebrate evolution. To gain a better understanding of the immune response in fish, we constructed a subtractive cDNA library from the head kidney of lipopolysaccharide-stimulated yellow catfish (Pelteobagrus fulvidraco) using suppression subtractive hybridization (SSH). A total of 300 putative EST clones were identified which contained 95 genes, including 27 immune-related genes, 7 cytoskeleton-related genes, 3 genes involved in the cell cycle and apoptosis, 9 respiration and energy metabolism-related genes, 7 genes related to transport, 24 metabolism-related genes, 10 genes involved in stress responses, seven genes involved in regulation of transcription and translation and 59 unknown genes. Using real-time quantitative reverse transcription PCR, a subset of randomly selected genes involved in the immune response to lipopolysaccharide challenge were investigated to verify the reliability of the SSH data which identified 16 up-regulated genes. The genes identified in this study provide novel insight into the immune response in fish.


Subject(s)
Catfishes/genetics , Catfishes/immunology , Fish Proteins/genetics , Immunity, Innate , Lipopolysaccharides/pharmacology , Animals , Catfishes/metabolism , DNA, Complementary/genetics , DNA, Complementary/metabolism , Fish Proteins/metabolism , Gene Library
15.
Org Lett ; 23(6): 2052-2056, 2021 03 19.
Article in English | MEDLINE | ID: mdl-33630600

ABSTRACT

The ruthenium-catalyzed remote ε-C-H alkylation of phosphines with tertiary alkyl halides has been developed. This novel PIII-directed C-H activation strategy tolerated various functional groups and delivered a wide variety of modified phosphines with excellent meta-site selectivity. Preliminary mechanistic studies indicated that a PIII-assisted ortho-cyclometalation/remote σ-activation pathway might be involved in this methodology.

16.
Org Lett ; 22(5): 2060-2063, 2020 Mar 06.
Article in English | MEDLINE | ID: mdl-32101014

ABSTRACT

The first example of Ir(III)-catalyzed C-H activation/cyclization with N-alkoxyamides as amidation reagents to simultaneously form functionalized thiadiazine 1-oxide derivatives was developed. This one-pot cascade protocol tolerated diverse functional groups and readily constructed various heterocyclic frameworks in moderate to good yield.

17.
Int J Biol Macromol ; 107(Pt A): 121-128, 2018 Feb.
Article in English | MEDLINE | ID: mdl-28866019

ABSTRACT

This study was performed to better understand the phylogenetic relationships within the lepidopteran superfamily Noctuoidea. The mitochondrial genome (mitogenome) has been extensively used for studying phylogenetic relationships at different taxonomic levels. In this study, the complete mitogenome of Spilarctia subcarnea (Noctuoidea: Erebidae) was sequenced and annotated. The mitogenome is 15,441bp in length, containing 13 typical protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a noncoding control region (CR). The order and orientation of genes of S. subcarnea mitogenome with the order trnM-trnI-trnQ-nad2 is different from the ancestral insects in which trnM is located between trnQ and nad2 (trnI-trnQ-trnM-nad2). The phylogenetic relationships based on mitochondrial sequences using Bayesian inference and Maximum likelihood methods showed that S. subcarnea was closely related to Lemyra melli, supporting that S. subcarnea belongs to Erebidae. These analyses confirm that Lymantriidae should be included as subfamilies within Erebidae. The Erebidae was sister to (Nolidae+(Euteliidae+Noctuidae)); Notodontidae is sister to the other families of Noctuoidea in our study.


Subject(s)
DNA, Mitochondrial/genetics , Genome, Mitochondrial/genetics , Lepidoptera/genetics , Phylogeny , Animals , Molecular Sequence Annotation , RNA, Ribosomal/genetics , RNA, Transfer/genetics
18.
Int J Biol Macromol ; 112: 1199-1207, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29454956

ABSTRACT

Antheraea pernyi is not only an important economic insect, it is increasingly employed as a model organism due to a variety of advantages, including ease of rearing and experimental manipulation compared with other Lepidoptera. Peptidoglycan (PGN) is a major component of the bacterial cell wall, and interactions between PGN and A. pernyi cause a series of physiological changes in the insect. In the present study, we constructed cDNA libraries from a A. pernyi PGN-infected group and a control group stimulated with phosphate-buffered saline (PBS). The transcriptome was de novo assembled using the Trinity platform, and 1698 differentially expressed genes (DEGs) were identified, comprising 894 up-regulated and 804 down-regulated genes. To further investigate immune-related DEGs, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were performed. GO analysis identified major immune-related GO terms and KEGG enrichment indicated gene responses to three pathways related to the insect immune system. Several homologous genes related to the immune response of the A. pernyi fat body post-PGN infection were identified and categorised. Taken together, the results provide insight into the complex molecular mechanisms of the responses to bacterial infection at the transcriptional level.


Subject(s)
Gene Expression Profiling , Gene Expression Regulation/drug effects , Moths/genetics , Peptidoglycan/pharmacology , Transcriptome/genetics , Animals , Base Sequence , Gene Ontology , Immunity/drug effects , Immunity/genetics , Molecular Sequence Annotation , Moths/drug effects , Moths/immunology
19.
PLoS One ; 12(6): e0179800, 2017.
Article in English | MEDLINE | ID: mdl-28622362

ABSTRACT

Mitochondrial genome (mitogenome) is very important to understand molecular evolution and phylogenetics. Herein, in this study, the complete mitogenome of Sesarmops sinensis was reported. The mitogenome was 15,905 bp in size, and contained 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region (CR). The AT skew and the GC skew are both negative in the mitogenomes of S. sinensis. The nucleotide composition of the S. sinensis mitogenome was also biased toward A + T nucleotides (75.7%). All tRNA genes displayed a typical mitochondrial tRNA cloverleaf structure, except for the trnS1 gene, which lacked a dihydroxyuridine arm. S. sinensis exhibits a novel rearrangement compared with the Pancrustacean ground pattern and other Brachyura species. Based on the 13 PCGs, the phylogenetic analysis showed that S. sinensis and Sesarma neglectum were clustered on one branch with high nodal support values, indicating that S. sinensis and S. neglectum have a sister group relationship. The group (S. sinensis + S. neglectum) was sister to (Parasesarmops tripectinis + Metopaulias depressus), suggesting that S. sinensis belongs to Grapsoidea, Sesarmidae. Phylogenetic trees based on amino acid sequences and nucleotide sequences of mitochondrial 13 PCGs using BI and ML respectively indicate that section Eubrachyura consists of four groups clearly. The resulting phylogeny supports the establishment of a separate subsection Potamoida. These four groups correspond to four subsections of Raninoida, Heterotremata, Potamoida, and Thoracotremata.


Subject(s)
Brachyura/genetics , Gene Rearrangement/physiology , Genes, Mitochondrial/physiology , Genome, Mitochondrial/physiology , Phylogeny , Animals , Arthropod Proteins/genetics , Mitochondrial Proteins/genetics , RNA/genetics , RNA, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/genetics
20.
Gene ; 627: 307-314, 2017 Sep 05.
Article in English | MEDLINE | ID: mdl-28636881

ABSTRACT

The mitochondrial genome (mt genome) provides important information for understanding molecular evolution and phylogeny. The further understand the molecular evolution and phylogeny of Helice tientsinensis, the complete mt genome was determined. It is 16,212bp long and includes 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes and a control region. The genome composition of H. tientsinensis was highly A+T biased 69.0% and showed negative AT skew (-0.017) and GC skew (-0.289). One PCG, all rRNAs and 12 of the tRNAs appeared to be rearranged with respect to the pancrustacean ground pattern gene order. Tandem duplication, followed by random deletion, is widely considered to explain translocation of mitochondrial genes. Consecutive recombinations events could explain inversions of genes. The phylogenetic analyses showed that H. tientsinensis has close relationships with Eriocheir japonica sinensis, E. j. hepuensis and E. j. japonica; this indicated that H. tientsinensis belongs in the Grapsoidea, part of the Varunidae family. This study provides evidence for a better understanding of gene rearrangements, as well as the evolutionary status of H. tientsinensis and related species.


Subject(s)
Brachyura/genetics , Gene Rearrangement , Genome, Mitochondrial , Phylogeny , Animals , Brachyura/classification
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