A novel method for analysis of single ion channel signal based on wavelet transform.

A single ion channel signal was analysed by the power distribution fraction constructed by a discrete wavelet transform. Average opening time and energy distribution of the signal can be obtained directly by this method. The method can also be used when the signal is corrupted by noise. By contrast, the conventional frequency domain analysis method--power spectral density--is less effective. Power distribution fraction will therefore give more useful information in analysis of experimental ion channel signals, principally by giving values of the mean channel opening time. The method may be applied to distinguish different ion channels more efficiently and to find their reactions to drugs.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: I. Site description and plant communities.

This paper describes the ecology of a coastal alluvial plain at Chichiriviche in northern Venezuela. The area supports a great diversity of plant communities, ranging from mangroves on the seaward edge of the plain to non-halophytic, fresh-water communities on the landward side. Small differences on topography result in a mosaic of saline and less-saline environments. Rainfall is strongly seasonal, causing superficial flooding of the alluvial plain in the rainy season and the creation of a hypersaline Substratum during the dry season. As a consequence, much of the plain is devoid or vegetation. Towards the landward side of the plain there are numerous small 'vegetation islands', fringed by halophilic succulent herbs, and made up of deciduous and semi-deciduous shrubs and trees together with non-halophytic CAM plants such as cacti and bromeliads. In subsequent papers the results of ecophysiological studies of these diverse plant species are presented.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: II. Cactaceae.

In an ecophysiological field investigation of plant communities of vegetation islands of an alluvial plain in northern Venezuela the members of the Cactaceae, Pereskia guamacho, and a columnar ceroid cactus, Subpilosocereus ottonis, were studied. The alluvial plain was flooded by fresh water in the rainy season in November/December 1985 and was dry and saline in the dry season in March/April 1986. The highly succulent leaves of P. guamacho were shed in the dry season. They performed C3 photosynthesis in the wet season and did not show signs of considerable salt accumulation. P. guamacho avoids stress due to drought and salinity by leaf-shedding. The columnar stem succulent ceroids are salt-excluding plants with crassulacean acid metabolism (CAM). Rapid die back and regeneration of absorptive roots, water parenchyma and CAM, with the possibility of nocturnal stomatal closure and CO2 recycling, are traits of adaptive value under fluctuating conditions between rainy and dry seasons on the alluvial plain.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: IV. Tillandsia flexuosa Sw. and Schomburgkia humboldtiana Reichb., epiphytic CAM plants.

A comparison of the performance of two epiphytes with crassulacean acid metabolism (CAM) was made during the rainy season and dry season at the Ciénega el Ostional, Chichiriviche in northern Venezuela. The epiphytic bromeliad, Tillandsia flexuosa has water-retaining tanks and leaf trichomes, and propagates mainly vegetatively to produce large populations in the shrubby island vegetation. The epiphytic orchid, Schomburgkia humboldtiana formed smaller populations, and had large succulent leaves with uniform chlorenchyma and no distinct water-storage parenchyma, unlike T. flexuosa. Both epiphytes were myrmecophilous. Leaf succulence (kg m-2 ) declined by ∼ 10% in the dry season for both plants. Both epiphytes showed reduced CO2 uptake during Phase I (dark period) and (dawn-dusk) titratable acidity (ΔH+ ) in the dry season. Water-use efficiency (WUE) was higher for S. humboldtiana (16.0 × 10-3 mol CO2 per mol H2 O compared with 5.0 × 10 -3 for T. flexuosa) although WUE remained constant during rainy and dry season for each species. Sixty to seventy per cent of the dawn dusk titratable acidity was derived internally from respiratory CO2 (recycling) for both species, and in absolute terms, recycling decreased in the dry season, in contrast to the expected progression under drought stress. Recycling is an important facet of carbon balance for both species in both rainy and dry seasons. Leaf Na+ concentration was higher than values quoted for terrestrial salt stressed CAM plants. Fructose and glucose declined in leaf bases of T. flexuosa during the dark period, but not in the more distal regions of the leaf. S. humboldtiana showed a decrease in sucrose at night, and mannitol was also an important constituent of the leaves. Xylem sap tension maxima increased from 0.38 ± 0.09 MPa (rainy season) to only 0.55 ± 0.06 MPa (dry season) for T. flexuosa, these values being much lower than those found for terrestrial shrubs and herbs at the same site, the two epiphytes use CAM in conjunction with differing morphological adaptations to maintain growth throughout the year at the Ciénega el Ostional, but it would seem that T. flexuosa has better physiological characteristics for maintenance of carbon acquisition during the dry season.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: VI. Water relations and gas exchange of mangroves.

Seasonal effects on leaf gas exchange and water relations were compared for Avicennia germinans, a true mangrove, and Conocarpus erectus, a mangrove associate, at coastal sites in northern Venezuela. On the Ciénega el Ostional at Chichiriviche, A. germinans was most abundant around lagoons on the seaward side of the vegetation-free alluvial sand plain. C. erectus was the dominant shrub in inland communities, but the two species co-occurred on vegetation islands at the landward edge of the alluvial plain. On the vegetation islands of the Ciénega el Ostional, gas exchange in A. germinans (a species with foliar salt glands) was less severely curtailed in the dry season compared with the rainy season than was gas exchange in C. erectus (a species lacking salt glands). Average rates of photosynthesis at near-saturating light intensities and total diurnal CO2 uptake were reduced in the dry season to 69 and 61%, respectively, of their values in the rainy for A. germinans, but to 48 and 30%, respectively, of their rainy-season values for C. erectus. Similarly, stomatal conductance and transpirational water loss were more reduced in the dry season for C. erectus than for A. germinans, with the result that C. erectus showed a 3.4-fold increase in water-use efficiency in the dry season compared with the rainy season. The importance of the soil environment in determining plant gas-exchange Patterns was evidenced by large seasonal shifts in dawn xylem tension for the two species (which increased from 1.34 MPa in the rainy season to 5.50 MPa in the dry season for A. germinans, and from 0.40 to 5.78 MPa for C. erectus). These values reflected changes in the soil environment caused by inundation of the upper soil layers by fresh water in the rainy season and a progressive increase in salt concentrations (to almost twice those in sea water) by evaporation from the soil in the dry season. Large changes in xylem tension were observed for both species during individual day-night cycles, reaching a maximum of 2.36 MPa for A. germinans. For C. erectus, the magnitude of these day-night changes was greatly reduced in the dry season, consistent with its very low transpiration rates at this time of year. Leaf-cell osmotic pressures also tended to be higher in A. germinans than C. erectus (attaining a maximum of 8.3 MPa for A. germinans in the dry season), and were related to the more seaward distribution of the true mangrove on the alluvial plain. Whereas leaves of A. germinans did not show any changes in succulence, leaf succulence in C. erectus increased with leafage and was slightly higher in the dry season than the rainy season. The more succulent leaves also had higher cell-sap osmotic pressures and NaCl concentrations. The most succulent leaves of C. erectus were observed for exposed shrubs growing on the shoreline. During the dry season, these shoreline plants showed high rates of gas exchange and low values for dawn xylem tension (0.89 MPa), indicating that they had access to relatively non-saline water from the shallow water table. On individual plants, exposed shoots had more succulent leaves and higher osmotic pressure and NaCl concentrations than sheltered leaves, demonstrating the importance of foliar absorption of salt borne in sea spray for the ionic relations of C. erectus. Thus, although the distribution of C. erectus is centred on brackish-water zones, this species can apparently extend from habitats with permanent access to a shallow water table through to areas where it is seasonally exposed to low soil water potentials and high salt concentrations in the substratum.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: III. Bromelia humilis Jacq., a terrestrial CAM bromeliad.

The terrestrial CAM plant Bromelia humilis was examined in the salinas of the Ciénega el Ostional, on the north coast of Venezuela, in the rainy and dry seasons. Three colour forms were distinguished; yellow (in full sun), green exposed (also in sun) and green shaded (beneath woodland). Plant size decreased with increasing irradiance. An examination was made of the three phenotypes in terms of CO2 exchange (Jco2 ), dawn-dusk changes in titratable acidity (ΔH+ ) and malate and citrate levels, osmotic pressure, xylem tension, sugar and amino acids levels, nitrogen and ion concentrations and ambient temperature fluorescence. All phenotypes exhibited lowered Jco2 and ΔH+ in the dry as compared to the rainy season. Citrate, as well as malate, showed dawn-dusk fluctuations. Soluble sugars were the major source of carbon skeletons for nocturnal organic acid production. The dawn-dusk changes in osmotic pressure were negligible. Yellow plants performed poorly in contrast to shaded plants in both seasons. The former showed higher dawn-dusk changes of citrate levels and contained much less nitrogen than shaded plants. Nocturnal recycling of respiratory CO2 was more important in yellow plants and, in the dry season, reached 87%. These differences were reflected in the overall productivity, shaded plants showing increases in size whereas yellow plants utilized energy mainly for leaf replacement. Water availability and nitrogen supply appear to be the overriding factors determining higher productivity and CO2 assimilation in partially shaded plants as compared with plants in full sun.

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: V. The Batis maritime-Sesuvium portulacastrum vegetation unit.

The perennial halophytes, Batis maritima L., Sesuvium portulacastrum L., and Portulaca rubricaulis H.B.K. which inhabit the extreme environment on parts of the salt plain and at the edges of the vegetation islands of Ciénega el Ostional were examined to determine their strategies for growth and survival in the rainy and dry seasons. All of the three species are leaf-succulents. High leaf-sap osmotic pressures, xylem tensions and Na+ and Cl- levels indicated that S. portulacastrum and B. maritima are salt-accumulating halophytes. Succulence, leaf-sap osmolalities and Na+ and Cl- levels increased in the dry season in B. maritima by a factor of 1.5-2.0 and in S. Portulacastrum by a factor of 1.9-2.7. B. maritima also accumulated sulphate with a two-fold increase of concentrations in the dry season. In S. portulacastrum Na+ accumulation much exceeded Cl- accumulation and oxalate synthesis was found to serve charge balance. In this species the compatible solutes, proline and pinitol, were clearly detectable in both seasons; their levels increased by a factor of about 6 in the dry season. Exchange of water vapour and CO2 was measured with a portable steady-state porometer. Photosynthesis in B. maritima showed little response to the transition from the rainy to the dry season while S. portulacastrum was severely impaired in the dry season, showing pronounced midday depressions of gas exchange and about 40% inhibition of light-saturated rates of CO2 uptake. P. rubricaulis shed its leaves in the dry season. According to carbon isotope ratios (δ13 C), B. maritima (δ13 C =-26.4‰) and S. portulacastrum (δ13 C =-25.8 ‰) are C3 plants while P. rubricaulis (δ13 C =-12.3‰) performed C4 photosynthesis.

Short term influx as a measure of influx across the plasmalemma.

It is shown that the influx of tracer to a plant cell must be measured for less than one third of the half-time for exchange of cytoplasmic tracer, and any subsequent wash must be negligibly brief, if the initial influx measured over a finite period is to be a good estimate of the plasmalemma influx. Complications due to lack of knowledge of the cytoplasmic exchange rate constant and to extracellular contents make it difficult to make such an estimate from influx measurements alone. The use of influx measurements is further discussed.