[Rapid isolation of 4-hydroxyphenylethanol from the bovine brain using Water Sep-Pak Plus cartridges with lipophilic sorbent (Florisil)]. / Bystroe vydelenie 4-gidroksifenilétanola iz mozga byka c ispol'zovaniem Waters Sep-Pak Plus kartridzhei s lipofil'nym sorbentom (Florisil).

4-Hydroxypherlylethanol, MAO-A Inhibitory component of endogenous MAO Inhibitory activity, tribulin, was isolated from a small quantity of bovine brain using Maters Sep-Pak Florisil cartridges. Procedure included isolation of crude tribulin fraction, which was passed through the cartridge. Preliminary data revealed that this substance is not absorbed on Florisil. The employment of this type of cartridges essentially accelerates isolation of 4-hydroxyphenyethanol from brain samples for quantitative determination. Pilot experiments also revealed that Sep-Pak Folrisil cartridges could be used for the rapid isolation of isatin, another endogenous MAO (B) inhibitor, from brain tissue.

[Involvement of monoamine oxidases in formation of 4-hydroxyphenylethanol, major component of tribulin A]. / Uchastie monoaminoksidaz v obrazovanii komponenta tribulina A 4-gidroksifenilétanola.

Pathways of 4-hydroxyphenylethanol formation were studied in the bovine brain. Incubation of brain homogenates with tyramine or tyrosine increased 4-hydroxyphenylethanol content only in the presence of NADH. Tyramine produced more pronounced augmentation of 4-hydroxyphenylethanol content than tyrosine. Tyrosine preparations were not contamined with tyramine. This suggests that 4-hydroxyphenylethanol may be formed from tyramine and tyrosine in NADH-dependent process. Preincubation of homogenates with 0.1 mM pargyline completely inhibited monoamine oxidases A and B and prevented NADH-dependent formation of 4-hydroxyphenylethanol from tyramine. The latter suggests that MAO is involved in formation of 4-hydroxyphenylethanol.

[The chromatographic analysis of phencyclidine, its metabolites and analogs in biological fluids]. / Khromatograficheskii analiz fentsiklidina, ego metabolitov i analogov v biologicheskikh zhidkostiakh.

Reviews data on analysis of narcotic phencyclidine and its main metabolites and analogs. Pharmacological and toxic characteristics of phencyclidine, conditions of its isolation from biological objects and its detection and measurement by thin layer and gas chromatography and by chromatographic mass-spectrometry are described. Analysis of phencyclidine metabolites is discussed and a scheme of its metabolism is presented. Statistical characteristics of methods of quantitative analysis of phencyclidine in biological objects are presented.

[The detection of tramadol and its metabolites in urine by chromatographic methods]. / Obnaruzhenie tramadola i ego metabolitov v moche khromatograficheskimi metodami.

The authors recommend detecting a compound drug tramadol and its metabolites in urinary extracts by thin-layer, gas-chromatographic, high-pressure liquid, and other chromatographic methods. The metabolites were identified with due consideration for their fragmentation during an electron strike thereof and their trimethylsilyl derivatives, mobility during separation in a thin layer, and results of selective extraction at various pH. The proposed scheme of biotransformation includes the formation of products of O- and N- demethylation, N, N- and N,O-didemethylation and hydroxylation of the cyclohexane fragment. The Rf metabolite values and their mass spectra are presented. During gas chromatographic analysis at an evaporator temperature at least 250 degrees C tramadol undergoes thermal destruction with the formation of water molecules and cyclohexane structure.

[The analysis of the methylenedioxy derivatives of amphetamine]. / ANaliz metilendioksiproizvodnykh amfetamina.

Methods for analysis of narcotics belonging to amphetamine methylene dioxy derivatives (MDD) are reviewed. The characteristics of these agents, their metabolism, and methods used for their detection and identification (TLC, GC, HPLC, GC/MS) are described. Methods for their extraction from biological objects (human urine and hair) are described. Efficacy of MDMA and MDEA from the urine by different extractants is assessed. The data demonstrate different potentialities for detection and identification of amphetamine MDD, including those in biological specimens (human urine and hairs), by numerous chromatographic methods.