RESUMEN
Two new triterpene saponins, namely notoginsenoside Ng5 (1) and notoginsenoside Ng6 (2) were isolated from the leaves of Panax notoginseng, along with five known ones. Their structures were determined by chemical methods, NMR and X-ray experiments. The absolute configuration of compound 3 with four sugar units was confirmed by single crystal X-ray analysis. Compounds 2-4 and 6 inhibited PC12 cell damage induced by serum deprivation, and increased cell viability from 58.7 ± 6.7% to 66.7 ± 4.5%, 76.1 ± 6.1%, 64.7 ± 5.2% and 67.2 ± 5.0% at 10 µM, respectively.
Asunto(s)
Fármacos Neuroprotectores/farmacología , Panax notoginseng/química , Hojas de la Planta/química , Saponinas/aislamiento & purificación , Saponinas/farmacología , Triterpenos/aislamiento & purificación , Triterpenos/farmacología , Animales , Espectroscopía de Resonancia Magnética con Carbono-13 , Supervivencia Celular/efectos de los fármacos , Conformación Molecular , Fármacos Neuroprotectores/química , Células PC12 , Espectroscopía de Protones por Resonancia Magnética , Ratas , Saponinas/química , Triterpenos/químicaRESUMEN
In view of the significant neuroprotective effect of Clausena lansium, we continued to separate the n-butanol and the water extracts from the stems of C. lansium in order to find the leading compounds with significant activity. Two new phenolic glycosides, Clausenolside A-B (1-2), one new pair of phenolic enantiomers (3a, 3b), and two new monoterpenoids, clausenapene A-B (4-5), together with twelve known analogues (6-17) were isolated from the stems of C. lansium. Compounds 1-17 were obtained from C. lansium for the first time. Compounds 3a, 3b, 4, 16, and 17 showed strong or moderate potential neuroprotective effects on inhibited PC12 cell injury induced by okadaic acid, and compound 9 exhibited strong potential hepatoprotective activities. Their structures were elucidated on the basis of spectroscopic analyses, including UV, IR, NMR experiments, and electronic circular dichroism (ECD) spectra.
Asunto(s)
Clausena/química , Medicamentos Herbarios Chinos/química , Tallos de la Planta/química , Animales , Supervivencia Celular/efectos de los fármacos , Descubrimiento de Drogas , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Células Hep G2 , Humanos , Hidrólisis , Monoterpenos/aislamiento & purificación , Monoterpenos/farmacología , Fármacos Neuroprotectores/aislamiento & purificación , Fármacos Neuroprotectores/farmacología , Células PC12 , Fenoles/aislamiento & purificación , Fenoles/farmacología , Ratas , EstereoisomerismoRESUMEN
Two new carbazole alkaloids, claulansine S (1) and claulansine T (2), and one new amide alkaloid, clauamide A (3), together with four known analogues (4-7) were isolated from the stems of Clausena lansium. Their structures were elucidated on the basis of spectroscopic analyses, including UV, IR, and NMR experiments (HSQC, HMBC, and NOE experiments). Compounds 4 and 6 showed moderate hepatoprotective activities.
Asunto(s)
Alcaloides/aislamiento & purificación , Amidas/aislamiento & purificación , Carbazoles/aislamiento & purificación , Clausena/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Alcaloides/química , Alcaloides/farmacología , Amidas/química , Amidas/farmacología , Carbazoles/química , Carbazoles/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Tallos de la Planta/química , Sustancias Protectoras/farmacologíaRESUMEN
Seven new carbazole alkaloids, claulansines L-R (1-7), and six known analogues (8-13) were isolated from the stems of Clausena lansium. Their structures were elucidated on the basis of spectroscopic analyses, including UV, IR, and NMR experiments (HSQC, HMBC, and NOE experiment). Compound 7 showed moderate anti-inflammatory activities. Compounds 3, 5, 6, 8, and 12 exhibited moderate hepatoprotective activities.