ABSTRACT
Cell-to-cell attachments or associations in expermentally induced sarcoma of chicken embryo lethal orphan virus (avian adenovirus) origin were studied in hamsters by electron microscopic examination. In many instances, the neoplastic cells seemed to be held together either by desmosome-like structures or an "interlocking" of their apposing plasma membranes. Less frequently, the cells were attached by button-like projections between the cell surfaces. Only rarely, interdigitation occurred between filopodial processes of cell surface of adjoining cells.
Subject(s)
Adenoviridae , Aviadenovirus , Cell Transformation, Neoplastic , Intercellular Junctions/ultrastructure , Sarcoma, Experimental/pathology , Animals , Cell Adhesion , Cell Membrane/ultrastructure , Cricetinae , Desmosomes/ultrastructureSubject(s)
Adenoma, Bile Duct/etiology , Adenoviridae/pathogenicity , Liver Neoplasms/etiology , Oncogenic Viruses , Peritoneal Neoplasms/etiology , Sarcoma, Experimental/etiology , Adenoma, Bile Duct/pathology , Animals , Animals, Newborn , Cricetinae , Hemolytic Plaque Technique , Injections, Intraperitoneal , Injections, Subcutaneous , Liver Neoplasms/pathology , Microscopy, Electron , Peritoneal Neoplasms/pathology , Sarcoma, Experimental/pathologySubject(s)
Azo Compounds/metabolism , Carcinogens/metabolism , Liver Neoplasms/chemically induced , Neoplasm Proteins/isolation & purification , Animals , Cytosol/analysis , Electrophoresis, Polyacrylamide Gel , Immunodiffusion , Liver/analysis , Liver Neoplasms/metabolism , Male , Molecular Weight , Neoplasm Proteins/immunology , Neoplasm Proteins/metabolism , Rabbits/immunology , RatsSubject(s)
Adenoviridae/pathogenicity , Neoplasms, Experimental/pathology , Animals , Animals, Newborn , Antigens, Viral/analysis , Cell Nucleus , Cell Transformation, Neoplastic , Cytopathogenic Effect, Viral , Endoplasmic Reticulum , Fluorescent Antibody Technique , Genetic Variation , Microscopy, Electron , Mitochondria , Mutation , Neoplasms, Experimental/immunology , Neoplasms, Experimental/microbiology , Oncogenic Viruses/isolation & purification , Oncogenic Viruses/pathogenicitySubject(s)
Chickens , Herpesviridae Infections/veterinary , Herpesviridae/isolation & purification , Poultry Diseases/microbiology , Tracheitis/veterinary , Virus Replication , Animals , Cells, Cultured , Chick Embryo , Herpesviridae/growth & development , Herpesviridae Infections/immunology , Herpesviridae Infections/microbiology , Injections , Kidney , Liver/microbiology , Lung/microbiology , Neutralization Tests , Poultry Diseases/immunology , Trachea/microbiology , Tracheitis/immunology , Tracheitis/microbiology , Viral Plaque AssaySubject(s)
Adenoviridae/growth & development , Chickens , Mutation , Animals , Cells, Cultured , Chick Embryo , Kidney , Microscopy, Electron , Time Factors , Viral Plaque Assay , Virus ReplicationSubject(s)
Herpesviridae/isolation & purification , Animals , Antigens , Cattle , DNA, Viral , Fluorescent Antibody Technique , Immune Sera , Kidney , Virus CultivationSubject(s)
Culture Techniques , Fowlpox , Poxviridae/growth & development , Animals , Chick Embryo , DNA , Fibroblasts , Methods , Staining and Labeling , Virus CultivationSubject(s)
Herpesvirus 1, Bovine/growth & development , Hydroxyurea/pharmacology , Virus Replication/drug effects , Animals , Antigens/analysis , Cattle , Cell Line , Cricetinae , Culture Techniques , Cytopathogenic Effect, Viral , DNA, Viral/analysis , DNA, Viral/antagonists & inhibitors , DNA, Viral/biosynthesis , Fluorescent Antibody Technique , Histocytochemistry , Inclusion Bodies, Viral , Kidney , Microscopy, Electron , Staining and Labeling , Viral Proteins/biosynthesisSubject(s)
Herpesvirus 1, Bovine/growth & development , Animals , Cattle , Cell Line , Cell Nucleus/microbiology , Cell-Free System , Cytoplasm/microbiology , Herpesvirus 1, Bovine/isolation & purification , Kidney , Methods , Microscopy, Electron , Time Factors , Virus Cultivation , Virus ReplicationABSTRACT
A 1,4-benzodiazepine, SC-32855, was administered orally once daily at doses of 30, 100, and 300 mg/kg for two weeks to adult Beagle dogs (1/sex/dose). The 300 mg/kg animals were sacrificed in extremis on day three after showing extreme signs of CNS depression. At necropsy, the testes of the 100 mg/kg male weighed considerably less than those of the control. The testicular weights of the 30 and 300 mg/kg animals were comparable to the weight of the control testes. Histopathologic evaluation of the testes of the 100 mg/kg male revealed degenerative and necrotic changes in the seminiferous tubules and cytoplasmic vacuolation in Leydig cells. In the 30 and 300 mg/kg animals, degenerative and necrotic changes were restricted to the seminiferous tubules.
Subject(s)
Anti-Anxiety Agents/toxicity , Benzodiazepines/pharmacology , Testicular Diseases/chemically induced , Animals , Body Weight/drug effects , Dogs , Male , Testis/pathologyABSTRACT
Ceroid, lipofuscin, and hyaline-type intracytoplasmic granules found in cardiac muscle cells of cynomolgus monkeys were studied using histologic, histochemical, and fluorescent microscopic techniques. The studies indicated that the ceroid granules contained an insoluble lipid as well as a component that was stainable with Luxol fast blue and Mallory's phloxine stain for hyaline. Lipofuscin granules had staining reactions characteristic of the classical age-related pigment. Hyaline granules were devoid of lipid component and were distinctly different from either ceroid or lipofuscin. All three types of granules were located at the poles of cardiac muscle cell nuclei.
Subject(s)
Cytoplasmic Granules/ultrastructure , Macaca fascicularis/anatomy & histology , Macaca/anatomy & histology , Myocardium/ultrastructure , Animals , Ceroid/analysis , Cytoplasmic Granules/analysis , Histocytochemistry , Hyalin/analysis , Lipofuscin/analysis , Mitochondria, Heart/ultrastructure , Mitochondrial Swelling , Myocardium/analysisABSTRACT
Arildone (also known as Win 38020), a novel aryl diketone, inhibited replication of herpes simplex virus type 2 in tissue culture by interfering with an event that occurs prior to 6 h postinfection. The inhibition could be partially reversed by washing. Although the exact mechanism of action is unknown, neither viral deoxyribonucleic acid nor viral proteins were synthesized in the presence of arildone.
Subject(s)
Antiviral Agents/pharmacology , Ketones/pharmacology , Animals , Centrifugation, Density Gradient , DNA/biosynthesis , DNA, Viral/biosynthesis , Haplorhini , In Vitro Techniques , Kidney/drug effects , Kidney/metabolism , Protein Biosynthesis , RNA/biosynthesis , Simplexvirus/drug effects , Simplexvirus/metabolism , Time Factors , Viral Plaque Assay , Viral Proteins/biosynthesis , Virus Replication/drug effectsABSTRACT
A mutant isolated from plaque purified type 1 avian adenovirus (CELO) differed from the parent virus in embryo pathogenicity and in plaque size.