RESUMEN
The adoptive transfer of antigen-specific T cells to establish immunity is an effective therapy for viral infections and tumors in animal models. The application of this approach to human disease would require the isolation and in vitro expansion of human antigen-specific T cells and evidence that such T cells persist and function in vivo after transfer. Cytomegalovirus-specific CD8+ cytotoxic T cell (CTL) clones could be isolated from bone marrow donors, propagated in vitro, and adoptively transferred to immunodeficient bone marrow transplant recipients. No toxicity developed and the clones provided persistent reconstitution of CD8+ cytomegalovirus-specific CTL responses.
Asunto(s)
Infecciones por Citomegalovirus/prevención & control , Linfocitos T Citotóxicos/inmunología , Vacunación/métodos , Antígenos de Diferenciación de Linfocitos T/inmunología , Trasplante de Médula Ósea/inmunología , Complejo CD3 , Linfocitos T CD4-Positivos/inmunología , Antígenos CD8/inmunología , Células Cultivadas , Humanos , Receptores de Antígenos de Linfocitos T/inmunologíaRESUMEN
A murine recombinant Neo(r) retrovirus encoding the SV40 small t antigen was used to infect Balb/c 3T3 CIA31 cells. From analyses of G418-resistant clones containing at least as much intact t as Cos-1 cells, we found that t, alone, had no detectable A31 transforming activity. In contrast, we noted that SV40 large T promoted A31 agar colony formation when present over a 5- to 7.5-fold concentration range. However, at the low end of the spectrum, its transforming effect was manifest inefficiently except in the presence of t. Thus a major role for t in the SV40 transforming mechanism is to enhance directly or indirectly the transforming function of T.