Complete chloroplast genome of the desert date (Balanites aegyptiaca (L.) Del. comparative analysis, and phylogenetic relationships among the members of Zygophyllaceae.

BACKGROUND: Balanites aegyptiaca (L.) Delile, commonly known as desert date, is a thorny evergreen tree belonging to the family Zygophyllaceae and subfamily Tribuloideae that is widespread in arid and semiarid regions. This plant is an important source of food and medicines and plays an important role in conservation strategies for restoring degraded desert ecosystems. RESULTS: In the present study, we sequenced the complete plastome of B. aegyptiaca. The chloroplast genome was 155,800 bp, with a typical four-region structure: a large single copy (LSC) region of 86,562 bp, a small single copy (SSC) region of 18,102 bp, and inverted repeat regions (IRa and IRb) of 25,568 bp each. The GC content was 35.5%. The chloroplast genome of B. aegyptiaca contains 107 genes, 75 of which coding proteins, 28 coding tRNA, and 4 coding rRNA. We did not observe a large loss in plastid genes or a reduction in the genome size in B. aegyptiaca, as found previously in some species belonging to the family Zygophyllaceae. However, we noticed a divergence in the location of certain genes at the IR-LSC and IR-SSC boundaries and loss of ndh genes relative to other species. Furthermore, the phylogenetic tree constructed from the complete chloroplast genome data broadly supported the taxonomic classification of B. aegyptiaca as belonging to the Zygophyllaceae family. The plastome of B. aegyptiaca was found to be rich in single sequence repeats (SSRs), with a total of 240 SSRs. CONCLUSIONS: The genomic data available from this study could be useful for developing molecular markers to evaluate population structure, investigate genetic variation, and improve production programs for B. aegyptiaca. Furthermore, the current data will support future investigation of the evolution of the family Zygophyllaceae.

Gene Losses and Plastome Degradation in the Hemiparasitic Species Plicosepalus acaciae and Plicosepalus curviflorus: Comparative Analyses and Phylogenetic Relationships among Santalales Members.

The Plicosepalus genus includes hemiparasitic mistletoe and belongs to the Loranthaceae family, and it has several medicinal uses. In the present study, we sequenced the complete plastomes of two species, Plicosepalus acaciae and Plicosepalus curviflorus, and compared them with the plastomes of photosynthetic species (hemiparasites) and nonphotosynthetic species (holoparasites) in the order Santalales. The complete chloroplast genomes of P. acaciae and P. curviflorus are circular molecules with lengths of 120,181 bp and 121,086 bp, respectively, containing 106 and 108 genes and 63 protein-coding genes, including 25 tRNA and 4 rRNA genes for each species. We observed a reduction in the genome size of P. acaciae and P. curviflorus and the loss of certain genes, although this reduction was less than that in the hemiparasite and holoparasitic cp genomes of the Santalales order. Phylogenetic analysis supported the taxonomic state of P. acaciae and P. curviflorus as members of the family Loranthaceae and tribe Lorantheae; however, the taxonomic status of certain tribes of Loranthaceae must be reconsidered and the species that belong to it must be verified. Furthermore, available chloroplast genome data of parasitic plants could help to strengthen efforts in weed management and encourage biotechnology research to improve host resistance.

Bioaccumulation and human health risk assessment of heavy metals in food crops irrigated with freshwater and treated wastewater: a case study in Southern Cairo, Egypt.

Food safety has often attracted attention worldwide. Few studies have investigated the heavy metal (HM) pollution and health risk assessment of crops and vegetables. The current work was conducted to evaluate the human risk assessment of HM (Cu, Cd, Cr, Pb, and Zn) in radish, lettuce, tomato, onion, turnip, squash, okra, sunflower, Jews mallow, and garden rocket cultivated in treated wastewater (TWW)-irrigated sites as compared with those cultivated in freshwater (FW)-irrigated sites. Irrigation water, soil, and different plants were collected from 6 farmlands irrigated with TWW and two agricultural sites irrigated with FW (Nile river). Heavy metal transfer factor (HMTF), chronic daily intake of metals (CDIM), health hazard risk (HR), and health hazard index (HI) were estimated. The results showed that the tested HM levels in FW and TWW were below the Food and Agriculture Organization (FAO) and Egyptian standards recommended for irrigation. In soil samples, HM levels were below the permissible limits for both tested sites. The HM in soil and plants grew in TWW-irrigated sites possessed multiple levels higher than those grown in FW-irrigated sites. Among different plants, HM levels in the edible parts of plants grown in TWW-irrigated sites followed in decreasing order: tomato > sunflower >Jew's mallow = turnip = squash > lettuce > okra = radish > onion > garden rocket. The mean CDIM and HR values of plants irrigated using TWW were higher than those irrigated using FW. Furthermore, HR values for all plants grown in polluted and unpolluted sites were < 1 except Cd in plants grown in the TWW-irrigated farmlands. The mean HI for radish, lettuce, tomato, onion, turnip, squash, okra, sunflower, Jews mallow, and garden rocket grown in TWW-irrigated sites were 2.08, 2.39, 1.76, 1.53, 2.08, 1.80, 2.03, 1.91, 1.82, and 1.44 (for adult), and 2.39, 2.75, 2.71, 1.75, 2.38, 2.06, 2.33, 2.69, 2.10, and 1.65 (for children). Plants irrigated with TWW showed a higher HMTF than plants irrigated with FW. Jew's mallow and okra irrigated with TWW had a maximum HMTF. Consequently, different practical measures can be taken to minimize the HM levels in agricultural foodstuffs. These measures include preventing the excessive application of pesticides and fertilizers for crop production and continuous monitoring of different foodstuffs in the market.

Taxonomic Revisiting and Phylogenetic Placement of Two Endangered Plant Species: Silene leucophylla Boiss. and Silene schimperiana Boiss. (Caryophyllaceae).

The genus Silene L. is one of the largest genera in Caryophyllaceae, and is distributed in the Northern Hemisphere and South America. The endemic species Silene leucophylla and the near-endemic S. schimperiana are native to the Sinai Peninsula, Egypt. They have reduced population size and are endangered on national and international scales. These two species have typically been disregarded in most studies of the genus Silene. This research integrates the Scanning Electron Microscope (SEM), species micromorphology, and the phylogenetic analysis of four DNA markers: ITS, matK, rbcL and psb-A/trn-H. Trichomes were observed on the stem of Silene leucophylla, while the S. schimperiana has a glabrous stem. Irregular epicuticle platelets with sinuate margin were found in S. schimperiana. Oblong, bone-shaped, and irregularly arranged epidermal cells were present on the leaf of S. leucophylla, while Silene schimperiana leaf has "tetra-, penta-, hexa-, and polygonal" epidermal cells. Silene leucophylla and S. schimperiana have amphistomatic stomata. The Bayesian phylogenetic analysis of each marker individually or in combination represented the first phylogenetic study to reveal the generic and sectional classification of S. leucophylla and S. schimperiana. Two Silene complexes are proposed based on morphological and phylogenetic data. The Leucophylla complex was allied to section Siphonomorpha and the Schimperiana complex was related to section Sclerocalycinae. However, these two complexes need further investigation and more exhaustive sampling to infer their complex phylogenetic relationships.