Interactive XCMS Online: simplifying advanced metabolomic data processing and subsequent statistical analyses.

XCMS Online (xcmsonline.scripps.edu) is a cloud-based informatic platform designed to process and visualize mass-spectrometry-based, untargeted metabolomic data. Initially, the platform was developed for two-group comparisons to match the independent, "control" versus "disease" experimental design. Here, we introduce an enhanced XCMS Online interface that enables users to perform dependent (paired) two-group comparisons, meta-analysis, and multigroup comparisons, with comprehensive statistical output and interactive visualization tools. Newly incorporated statistical tests cover a wide array of univariate analyses. Multigroup comparison allows for the identification of differentially expressed metabolite features across multiple classes of data while higher order meta-analysis facilitates the identification of shared metabolic patterns across multiple two-group comparisons. Given the complexity of these data sets, we have developed an interactive platform where users can monitor the statistical output of univariate (cloud plots) and multivariate (PCA plots) data analysis in real time by adjusting the threshold and range of various parameters. On the interactive cloud plot, metabolite features can be filtered out by their significance level (p-value), fold change, mass-to-charge ratio, retention time, and intensity. The variation pattern of each feature can be visualized on both extracted-ion chromatograms and box plots. The interactive principal component analysis includes scores, loadings, and scree plots that can be adjusted depending on scaling criteria. The utility of XCMS functionalities is demonstrated through the metabolomic analysis of bacterial stress response and the comparison of lymphoblastic leukemia cell lines.

[Endophthalmitis by Aspergillus fumigatus after retina detachment]. / Endoftalmitis por Aspergillus fumigatus tras desprendimiento de retina.

A fungal infection in the right eye after retina detachment on an immunocompetent patient is reported. After surgery, she developed an infection that was empirically treated with antibiotics and corticoids. Later the patient developed another retina and choroid detachment. The infection evolved to endophthalmitis and a sample was sent to the microbiology laboratory, where Aspergillus fumigatus was isolated. In spite of treatment with intravenous and intravitreous amphotericin B, the eye was eventually removed by enucleation.

Bilateral type III Duane syndrome: a case report.

PURPOSE: Duane syndrome (DS) belongs to a group of congenital retraction syndromes, the incidence of which varies between 2% and 4% in patients with strabismus. In most cases the damage is unilateral, but it manifests as bilateral in about 20% of cases. According to the classification of Huber, type III DS exhibits a limitation of abduction and adduction. METHODS: We report the case of a 5-year-old boy from Eastern Europe who was taken to the hospital because he was unable to move both eyes horizontally from birth and had not been previously seen by an ophthalmologist. RESULTS: The examination showed inability to abduct and adduct bilaterally, with narrowing of the palpebral fissures on attempted adduction. The patient was diagnosed with bilateral type III DS. CONCLUSIONS: Treatment is individualized on a case-by-case basis. For our patient, monitoring by way of regular checkups was chosen, with the parents rejecting surgery for now.