A fully digital workflow involving 3D printed tooth reduction guides and injection resin-indexes to restore an impacted canine.

OBJECTIVE: The present clinical report describes a long-term temporary restorative approach using injectable composite to reshape a canine into a central incisor, within the context of an ongoing orthodontic treatment. This treatment protocol describes a fully-digital workflow, incorporating digital designed and 3D printed diagnostic wax up, reduction guides and resin-injection index. CLINICAL CONSIDERATIONS: Effective planning is important when aiming to deliver a comprehensive and multidisciplinary workflow, and digital tools serve as invaluable aids. CONCLUSION: The use of a fully digital workflow in a comprehensive dental treatment resulted in a predictable and successful outcome for a restoration of a canine that was transformed into a central incisor. CLINICAL SIGNIFICANCE: This approach highlights the efficacy of digital technology in achieving precise and successful dental restorations, emphasizing its significance in modern dental practice.

In vitro comparative study between complete arch conventional implant impressions and digital implant scans with scannable pick-up impression copings.

STATEMENT OF PROBLEM: Intraoral digital scan techniques have been widely used and sufficient evidence supports this technique in partially edentulous patients. However, the evidence supporting the use of intraoral scanners (IOSs) for edentulous patients is limited. PURPOSE: The purpose of this in vitro study was to measure and compare the accuracy of complete arch conventional pick-up implant impressions with open and closed trays, complete arch digital implant scans with IOSs, and 3-dimensional (3D) printed casts from complete arch digital implant scans. MATERIAL AND METHODS: Six implants were placed in a mandibular model. Scannable pick-up impression copings were inserted in the implants, scanned with a reference scanner, and exported in standard tessellation language (STL) format (Group Control). Splinted open-tray pick-up impressions (Group OT, n=5) and closed-tray pick-up impressions (Group CT, n=5) were made, and stone casts were fabricated. Digital scans (Group DS, n=5) were made with an IOS, and the STL files were exported to fabricate 3D printed casts (Group STL, n=5). Scannable pick-up impression copings were inserted in the dental implant analogs in Groups OT, CT, and STL and scanned with the reference scanner. Using a 3D inspection software program, the recording techniques were compared with the control. Root mean square (RMS) values were calculated from the control, and superimposed digitized casts from different recording techniques. Analysis of variance was used to determine differences in RMS values, and theTukey post hoc test was used to determine difference between different groups. RESULTS: Group CT had the lowest mean dimensional difference when superimposed with Group Control, followed by Groups DS, OT, and STL. Significant differences were found in RMS values between Control and digitized casts fabricated with different techniques (P<.05). The post hoc Tukey test revealed that Group DS (P<.05) was significantly different from the other groups, while no significant difference was found among Groups CT, OT, and STL (P>.05). CONCLUSIONS: Based on the findings of the present study, 3D printed casts from digital scans have the same accuracy as stone casts from conventional impressions in complete arch implant cases. Intraoral scans had the highest accuracy. Complete arch pick-up impression techniques using dual-functioning scannable pick-up impression copings are as accurate as splinted complete arch pick-up impressions.

Implant position and guided placement for in-house-produced CAD/CAM restorations with titanium (Ti)-bases.

Digital dentistry has contributed to the evolution and simplification of dental implantology over the last decade. The incorporation of intraoral scanners, CBCT, 3D implant-planning software, and CAD/CAM systems makes prosthetically driven implantology a straightforward process. Such digital resources for treatment planning and execution, following evidence-based concepts, have the ability to improve the long-term esthetics and function of implant-supported restorations as well the long-term survival of dental implants. Dental implants are frequently considered as the first treatment option for replacing failing or missing teeth. However, their use in the esthetic zone remains a challenge for many clinicians. The present article provides clinical guidelines for ideal implant positioning employing computer-guided surgery and chairside CAD/CAM-fabricated provisional and definitive restorations with titanium (Ti)-bases for successful prosthetic outcomes, optimizing gingival architecture, and decreasing overall treatment duration. (Int J Comput Dent 2023;26(1):75-0; doi: 10.3290/j.ijcd.b3818287).

The Computer Aided Design/press technique: Fabrication of zirconia-reinforced lithium disilicate restorations for treatment of extensive noncarious cervical lesions.

OBJECTIVE: The aim of this case report is to present the CAD/Press technique; a workflow that combines digital design, milled, or 3D printed burnout molds, and a new pressable ceramic material for the treatment of extensive noncarious lesions with minimal invasive indirect ceramic restorations. CLINICAL CONSIDERATIONS: Clinical decisions from material selection, preparation design, manufacturing techniques to adhesive protocols will be discussed, as they are important factors for achieving long-term clinical success while preserving healthy tooth structure. CLINICAL SIGNIFICANCE: The presented workflow aims to ease clinical decisions when restoring extensive cases with indirect restorations and show clinicians a workflow combining several techniques.

Analysis of the mesh resolution of an .STL exported from an intraoral scanner file.

OBJECTIVES: This study aimed to provide information on the accuracy of exported digital files with the different resolutions available in the CEREC 4.6.2 software obtained by means of an intraoral scanner (IOS), in addition to establishing differences between materialized models with different exported resolutions, and how these different exported files can influence finite element analysis (FEA) simulations. MATERIALS AND METHODS: The upper complete arch of 10 patients was scanned through an IOS (CEREC Omnicam 1.0/Dentsply Sirona). Files of three resolution meshes digitalized by a CAD software (Cerec SW, 4.6.2) high, medium and low (IOSH, IOSM, and IOSL) were exported. Each file was evaluated by a software (NETFABB) about the number of triangles obtained and compared with the number announced by the manufacturer. Also, with a superimposition with a specialized software (GEOMAGIC X), the digital models were compared. The files of each resolution were printed (Sprintray 3D Printer), and the printed models were scanned with IOS (Omnicam 1.0) and compared with the control group (intraoral scanned high-resolution file, IOSH). FEA simulations were imported into COMSOL and analyzed under different loading conditions. RESULTS: The number of exported triangles coincided with that reported by the manufacturer. The digital models from files of different resolution did not show significant differences (less than 1.5 um) between each other. Models printed (H, M, L) from files of the same resolution mesh (H, M, L) did not show significant differences between them either in partial measures of the arch and neither in the complete arch. FEA showed significant differences in stress concentration between different exported models. CLINICAL SIGNIFICANCE: Digital models can be exported and printed in three resolutions of the mesh, without differences clinically significative. On the other hand, for future FEA applications further research should be performed in order to determine the optimal number of triangles.

3D-printed resins for provisional dental restorations: Comparison of mechanical and biological properties.

OBJECTIVES: To characterize the mechanical and biological properties of three commercially available resins, which are currently used for provisional restorations and to compare them to an experimental resin intended for definitive fixed dental prostheses. MATERIALS AND METHODS: Three commercially available resins: Crowntec (CT, Saremco), Temporary C&B (FL, Formlabs), C&B MFH (ND, Nextdent), and the experimental resin: Permanent Bridge (PB, Saremco) were printed and subjected to biaxial flexural strength test, finite element analysis, Weibull analysis, scanning electron microscopy, cell proliferation, immunohistochemistry and cytotoxicity assays. Samples from CT, PB, and ND were provided directly from the manufacturers ensuring ideal workflow. FL was printed using the workflow as recommended by the manufacturer, using a Formlabs 2 printer and their post-processing units Form Wash and Form Cure. RESULTS: From the tested resins, PB yielded the best overall results in terms of mechanical properties. Cell proliferation and cytotoxicity did not show any significant differences among materials. PB showed higher values for probability of survival predictions (35%) when subjected to 250 MPa loads, whereas the other materials did not reach 10%. SIGNIFICANCE: Despite mechanical differences between the evaluated materials, the outcomes suggest that 3D printed provisional resins may be used in clinical settings, following the manufacturers indications. New materials intended for long-term use, such as the PB resin, yielded higher mechanical properties compared to the other materials. Alternative printing and post-processing methods have not yet been evaluated and should be avoided until further literature is available. CLINICAL SIGNIFICANCE: 3D printed resins for provisional restorations have become popular with the emergence of new technologies. In this study, we evaluated three different commercially available resins for provisional restorations and one new experimental resin. The results from this study indicate that commercially available resins could be used in clinical settings under certain conditions and limited periods of time. Following the manufacturers protocols is of paramount importance to not compromise these properties.

Full digital workflow to resolve angled adjacent dental implants: A dental technique.

The digital acquisition, fabrication process, and delivery of computer-aided design and computer-aided manufacture (CAD-CAM) implant-supported restorations on angled adjacent implants are described. The proximal surface of a scan post was modified for correct adaptation, permitting an accurate digital scan of adjacent implants in 1 step. Definitive screw-retained splinted implant-supported restorations were designed and milled in a zirconia material and delivered with a combined extraoral and intraoral cementation protocol.

A comparison of ceramic crown color difference between different shade selection methods: Visual, digital camera, and smartphone.

STATEMENT OF PROBLEM: The light source stability of digital cameras and smartphones is important in shade matching in restorative and prosthetic dentistry to communicate objectively with the dental laboratory. Techniques that standardize the light source of such devices are lacking, and this limitation can lead to color mismatches, difficulties in color communication, and treatment documentation. PURPOSE: The purpose of this clinical study was to compare the magnitude of color difference (ΔE) among 3 shade selection methods during the fabrication of ceramic crowns: visual shade selection with a shade guide, digital shade selection with a digital camera and cross-polarizing filter, and digital shade selection with a smartphone and a light-correcting device. MATERIAL AND METHODS: Forty-five patients in need of ceramic crowns were enrolled, and shade selection was evaluated according to different protocols: visual shade selection (A-D shade guide and IPS Natural Die Material Shade Guide, sent to the dental laboratory technician via a laboratory prescription); digital shade selection with a digital camera (D7000; Nikon Corp) with an 85-mm lens and wireless close-up flash, with and without a cross-polarizing filter (Polar eyes); and digital shade selection with a smartphone and a light-correcting device (iPhone XS attached to Smile Lite MDP, with and without its cross-polarizing filter accessory). Information from the smartphone was imported to an app (IPS e.max Shade Navigation App; Ivoclar AG) that converted the reading to a shade and level of translucency for the ceramic restoration. For all photographs, a gray reference card with known color values was positioned by the mandibular teeth and was used for white balancing of the digital photographs with a software program. All photographs were edited and sent to the dental laboratory: white-balanced with the shade guide; white-balanced with the substrate shade guide; black and white; saturated; and cross-polarized. Ceramic crowns were made with the same lithium disilicate material (IPS e.max CAD; Ivoclar AG) and cemented with the same resin cement (RelyX Ultimate Clicker, A3 shade; 3M). The ΔE values between the crown and the adjacent tooth were determined. The data were analyzed by using a 1-way analysis of variance (ANOVA) and Tukey post hoc tests (α=.05). RESULTS: The mean ΔE between a cemented ceramic crown and the adjacent tooth in the visual shade selection group was 5.32, significantly different than both digital camera (ΔE=2.75; P=.002) and smartphone (ΔE=2.34; P=.001), which were not different from each other (P=.857). CONCLUSIONS: The digital shade selection with photographs acquired with both a digital camera and a smartphone with a light-correcting device showed a threshold within the acceptable values (ΔE<3.7), whereas the visual shade selection showed an average ΔE above the threshold for acceptable values (ΔE>3.7). The use of a gray reference card helped standardize the white balance from the digital images.

Microcomputed tomography evaluation of cement film thickness of veneers and crowns made with conventional and 3D printed provisional materials.

OBJECTIVE: To evaluate, through microcomputed tomography (µCT), the cement film thickness of veneers and crowns made with different provisional materials. MATERIAL AND METHODS: A veneer and a crown preparation were performed on a central incisor and a second molar of a dental model, respectively, scanned with an intraoral scanner, and the .stl files were exported to an LCD-based SLA three-dimensional (3D)-Printer. Twenty-four preparations were 3D-printed for each veneer and crown and divided into four groups (n = 6/group): (a) Acrylic resin (Acrílico Marche); (b) Bisacrylic resin (Protemp 4); (c) PMMA computer-aided design and computer-aided manufacturing (CAD-CAM) (Vipiblock); and (d) 3D-printed resin for provisional restorations (Raydent C&B for temporary crown and bridge). Veneers and crowns restorations were performed and cemented with a flowable composite. Each specimen was scanned with a µCT apparatus, files were imported for data analysis, and cement film thickness was quantitatively measured. Data were analyzed by 2-way ANOVA and Tukey post-hoc tests (α = .05). RESULTS: Crowns presented a thicker cementation film than veneers (P < .05).The bisacrylic resin showed the smallest veneer film thickness, similar to the acrylic resin (P = .151), which was not significantly different than the PMMA CAD/CAM material (P = .153). The 3D printed provisional material showed the thicker film, different than all other materials (P < .05). The bisacrylic resin showed a cement film thickness with a high number of voids in its surface. For crowns cementation, the 3D printed provisional material showed the thicker cementation film, different than all other materials (P < .05). CONCLUSIONS: Different provisional materials present different film thicknesses. The 3D printed provisional material showed the highest veneer and crown film thicknesses. Veneers film thicknesses were smaller than crowns for all provisional materials. CLINICAL SIGNIFICANCE: The 3D printed provisional material studied can be satisfactorily used, presenting appropriate adaptation with the tooth preparation, however, it shows the highest cement film thickness for both veneers and crowns cementations when compared with other provisional materials. A better internal fit, or smaller cement film thickness is obtained by CAD/CAM materials, acrylic and bisacrylic resins. Veneer cementation showed a smaller cement film thickness compared with crown cementation for all provisional materials.

Effect of different fabrication steps on color and translucency of a CAD-CAM feldspathic ceramic.

OBJECTIVE: To evaluate color differences (ΔE) of different fabrication steps performed on a feldspathic ceramic and relative translucency parameter (RTP) after glazing; and to evaluate their effects on the color parameters: L*, a* and b*. MATERIAL AND METHODS: Computer-aided design/computer-aided manufacturing (CAD-CAM) feldspathic maxillary right central incisor ceramic crowns (Cerec Blocs; 1.4 mm thickness) were fabricated through scanning a model preparation and milling. A total of 20 specimens were used and different parameters were compared with each other for color difference (ΔE): original block, milling, polishing, glazing and try-in cements; RTP was measured after glazing. Color parameters (L*, a*, and b*) were evaluated after each step with a spectrophotometer (Vita Easyshade V) and compared using the CIEDE2000 formula. Statistical analyses were done using one-way ANOVA and post-hoc tests. RESULTS: The highest ΔE value was observed for Original versus Milling group (4.73) and the lowest for Polishing versus Glazing (0.49). RTP after glazing presented a value of 12.01. L*, a*, and b* parameters were different among groups. Milling group was the one that presented more differences compared to the Original block regarding L*, a*, and b* parameters. CONCLUSIONS: Different fabrication steps resulted in perceptible color differences within themselves, and L*, a*, and b* parameters changed according to each step. All milling specimens presented unacceptable color differences when compared to the other steps, and milling specimens showed all color parameters (L*, a*, and b*) statistically different compared to the Original block. A high RTP value was observed, demonstrating that, at 1.4 mm thickness, the feldspathic ceramic presented high translucency, showing that opacification of substrate might be necessary. CLINICAL SIGNIFICANCE: Different fabrication steps can greatly affect the optical properties of the feldspathic ceramic restoration studied and cause a relevant impact when compared to the ceramic block itself. Crowns evaluated right after milling presented the highest color differences. In order to obtain optical properties as close as possible to the original feldspathic ceramic block, either polishing or polishing and glazing needs to be performed.

Effect of surface treatment and cleaning on the bond strength to polymer-infiltrated ceramic network CAD-CAM material.

STATEMENT OF PROBLEM: Optimal composite resin bonds to polymer-infiltrated ceramic network (PICN) computer-aided design and computer-aided manufacturing (CAD-CAM) materials are essential for clinical success. However, comparative bond strength studies on the influence of different etching and cleaning methods on these materials are lacking. PURPOSE: The purpose of this in vitro study was to measure and compare the microshear bond strength (µSBS) of a composite resin cement with that of a PICN material after different surface treatment and cleaning methods. MATERIAL AND METHODS: Seventy specimens of a CAD-CAM PICN were divided into 7 groups (n=10): no treatment (control), hydrofluoric acid etching for 20 seconds (HF20), 60 seconds (HF60), 120 seconds (HF120), HF20 + phosphoric acid for 60 seconds and ultrasonic bath for 5 minutes, HF60 + PH, and HF120 + PH. After surface treatment, a silane coupling agent and composite resin cement were applied. Microshear bond strength was determined, and data were analyzed with 1-way ANOVA and Tukey post hoc multiple comparison tests (α=.05). RESULTS: All HF acid treatments resulted in a significant increase in bond strength to the polymer-infiltrated ceramic network material (P=.02). Bond strength values for HF etching for 20 seconds were significantly lower than those for 60 seconds and 120 seconds (P=.034). No difference was found between 60 seconds and 120 seconds of HF etching time (P=.986). Additional surface treatment with phosphoric acid 60 seconds and ultrasonic bath 5 minutes did not improve the bond strength beyond values obtained by hydrofluoric acid treatment only (P=.834). Most failures were cohesive. CONCLUSIONS: Acid etching and surface treatment have significant effects on composite resin bond strength to a PICN CAD-CAM material. HF etching for 60 seconds or 120 seconds provides the highest bond strengths. Cleaning methods after etching did not have any significant effect on bond strength.

Multidisciplinary clinical approach of an orthodontic treatment and complete digital rehabilitation of laminate veneers for esthetically compromised worn teeth.

AIM: The present clinical case reports on the workflow of a multidisciplinary treatment including both orthodontic and esthetic procedures for the rehabilitation of a young patient with severe wear in the anterior area of both the maxillary and mandibular arches due to malocclusion. The described workflow comprises a digital step-by-step process that involves 3D printing, intraoral scanners, and CAD/CAM milling systems. RESULTS: Accurate diagnostic and treatment planning were paramount when different specialties needed to combine and work together. For that purpose, different digital procedures helped to improve the predictability and the dynamic of the orthodontic and restorative treatments. CONCLUSION: The present multidisciplinary clinical case was executed with a digital approach that assisted to speed things up and achieve a predictable treatment option.

Copy milling to duplicate the emergence profile for implant-supported restorations.

This technique article describes the duplication of a definitive implant-supported computer-aided design and computer-aided manufacture (CAD-CAM) crown with soft-tissue modification on a misplaced implant. Gingival contours were ideally shaped in the stone die, and a custom healing abutment was designed. After scanning the modified surface, images were correlated with the initial intraoral digital scan, and the definitive restoration was designed, milled, and delivered with an ideal soft-tissue emergence profile.

In vitro evaluation of surface roughness, color stability, and color masking of provisional restoration materials for veneers and crowns.

AIM: To evaluate surface roughness (SR), color stability (CS), and color masking as assessed through the relative translucency parameter (RTP) of different provisional restoration materials before and after water thermocycling (TC). MATERIALS AND METHODS: Four different provisional materialas were selected: acrylic resin, bis-acryl resin, polymethyl methacrylate (PMMA) CAD/CAM blocks, and 3D-printed provisional resin. Samples of 0.6- and 1.3-mm thickness were obtained, and SR, CS, and RTP were determined before and after the samples were submitted to 6000 cycles of TC. A rugosimeter was used to assess SR. Color was determined on white and black backgrounds before and after TC, and color masking was assessed through RTP. CS was determined using a spectrophotometer with the CIEDE2000 formula, before and after TC. RESULTS: SR values before and after TC ranged from -0.01 to 0.28 for 1.3 mm, and from 0.00 to 0.38 for 0.6 mm, respectively. CS (ΔE00 values) ranged from 0.53 to 4.38 for 1.3 mm, and from 0.70 to 5.66 for 0.6 mm, respectively. The highest value was obtained for 3D-printed resin. Finally, for RTP values, the difference before and after TC ranged from -0.036 to 2.66 for 1.3 mm, and from 0.52 to 2.57 for 0.6 mm, respectively, with the PMMA CAD/CAM block being the material with the lowest values in both cases. CONCLUSION: Values of SR, CS, and RTP varied among different materials and thicknesses. The overall performance of 3D-printed resin was inferior to ?that of the other materials. The PMMA CAD/CAM block obtained the overall best values for the conditions tested.

Variability of color matching with different digital photography techniques and a gray reference card.

STATEMENT OF PROBLEM: Color matching in restorative and prosthetic dentistry is important for the success of dental treatments, although communication with the dental laboratory remains subjective, and studies of the performance of objective communication methods are lacking. PURPOSE: The purpose of this study was to evaluate color differences (ΔE) and compare color luminosity (L*) values among different types of digital photography equipment used to document tooth color, with and without a gray reference card, and to determine whether the gray card could be used to standardize color assessment in dental photography. MATERIAL AND METHODS: Sixty photographs were made (n=10) using different equipment: a D7000 digital camera (Nikon Corp) with an 85-mm lens and wireless close-up flash (DC+WCF); close-up flash surrounded by 80 grams per square meter (gsm; specification of paper thickness) white printing paper (DC+WPP); ring flash (DC+RF); close-up flash attached to a dual-point rigid flash bracket (DC+DPRF); cross-polarizing filter attached to a close-up flash (DC+CPF); and iPhone 7 (I7). For all photographs, a gray reference card with known color values was positioned at the patients' mandibular teeth, acting as a parameter for the analysis of white-balanced digital photographs. Each photograph underwent white balance with the reference card and software. ΔE were obtained from each piece of equipment by comparing images with and without white balance (original photo) with software and the smallest ΔE achieved was used as the gold standard for comparisons of luminosity. Values of luminosity were subsequently obtained for the different equipment with and without white balancing the photographs; these values were compared using a general estimating equation with Huber-White standard error (α=.05). RESULTS: The use of a cross-polarizing filter was used as the gold standard for luminosity evaluation, as the smallest ΔE (3.4) among photographs were observed when those with and without white balance were compared. Luminosity results from the cross-polarizing filter method (DC+CPF) were not significantly different from those of the DC+DPRF (P=.73), DC+WPP (P=.106), and DC+WCF (P=.551) groups but were statistically different from DC+RF (P=.028) and I7 groups (P<.001). Use of a gray card was significant when a ring flash (P=.008) or the iPhone (P=.023) were used but not statistically significant for the other groups (P>.05). CONCLUSIONS: The use of a cross-polarizing filter results in more color-standardized photographs, while the ring flash system and the iPhone 7 result in less standardized photographs. The gray reference card had a significant effect when a ring flash system or iPhone 7 was used.

Void and gap evaluation using microcomputed tomography of different fiber post cementation techniques.

STATEMENT OF PROBLEM: Few studies have investigated the voids and gaps produced during the cementation of fiber posts using different techniques. PURPOSE: The purpose of this study was to evaluate and quantify void and gap area formations of different fiber post cementation techniques using microcomputed tomography (µCT). MATERIAL AND METHODS: Standardized endodontically treated acrylic resin roots (N=24) were divided into 4 groups (n=6) according to different fiber posts cemented with the resin cement (FB); fiber posts relined with composite resin followed by cementation (FBR); fiber posts cemented using an ultrasonic device (FBU); and fiber posts relined with composite resin and cemented using an ultrasonic device (FBRU). Each specimen was scanned twice using micro-computed tomography (µCT; empty root, followed by after fiber post cementation). Digital imaging and communications in medicine (DICOM) files were transferred into 3-dimensional (3D) reconstruction software for analysis. Void volume in the cementation system and gap area formation were evaluated; quantitative and qualitative analyses were performed. The data were analyzed using 2-way ANOVA and the Tukey honest significant difference post hoc test (α=.05). RESULTS: FBR showed a lower percentage of voids than obtained for FB (P<.05). Groups FB, FBU, and FBRU did not show significant difference in void formation (P>.05). No significant differences were found in gap area formations among the experimental groups (P>.05). CONCLUSIONS: The use of a composite resin to reline the fiber post significantly decreased the void formation in the cementation procedure when no ultrasonic device was used. The use of an ultrasonic device did not decrease the percentage of void or gap formation for any technique evaluated.

Preliminary Evidence for the Complete Digital Esthetic Rehabilitation Treatment: Case Report and 1-Year Follow-up.

PURPOSE: To show through computer-aided design and manufacturing technology combined with novel digital acquisition images and the right restorative material that the technology can provide an easier and predictable workflow for general practitioners. BASIC PROCEDURE: This case report presents a fully digital treatment of a 59-year-old patient with tooth-supported crowns and provides a detailed step-by-step digital technique to obtain a successful result. CONCLUSION: By avoiding traditional impression and fixed prosthetic techniques, excellent clinical results can be obtained, minimizing the required steps and simplifying the overall procedure. This adds information to the current literature, allowing general practitioners to choose the best treatment option. The function and esthetics remained unaltered over a 1-year follow-up period. No fracture or decementation was observed.

Skeletal adaptation to mechanical cues during homeostasis and repair: the niche, cells, and molecular signaling.

Bones constantly change and adapt to physical stress throughout a person's life. Mechanical signals are important regulators of bone remodeling and repair by activating skeletal stem and progenitor cells (SSPCs) to proliferate and differentiate into bone-forming osteoblasts using molecular signaling mechanisms not yet fully understood. SSPCs reside in a dynamic specialized microenvironment called the niche, where external signals integrate to influence cell maintenance, behavior and fate determination. The nature of the niche in bone, including its cellular and extracellular makeup and regulatory molecular signals, is not completely understood. The mechanisms by which the niche, with all of its components and complexity, is modulated by mechanical signals during homeostasis and repair are virtually unknown. This review summarizes the current view of the cells and signals involved in mechanical adaptation of bone during homeostasis and repair, with an emphasis on identifying novel targets for the prevention and treatment of age-related bone loss and hard-to-heal fractures.

Repair Bond Strength of Aged Composite Resins Using Different Surface Treatment Protocols.

This study evaluated shear bond strength (SBS) of thermally aged composite resins repaired using different surface protocols. Four-hundred composite resin samples were made using the following materials (100 samples per material): Filtek Z350XT (FXT); Spectra Smart (SSM); IPS Empress Direct (EDI); and Forma (FOR). Each group's samples were then divided into 10 groups (n = 10 samples per group): G1: no surface treatment; G2: phosphoric acid-etching + universal-adhesive (PU); G3: surface roughening + PU (RPU); G4: RPU + silane (RPSU); G5: surface roughening + hydrofluoric acid-etching + universal adhesive (RHU); G6: RHU + silane (RHSU); G7: dry sandblast + PU (DsPU); G8: DsPU + silane (DsPSU); G9: wet sandblast + PU (WsPU); and G10: WsPU + silane (WsPSU). G1 was freshly repaired, and G2 to G10 were thermally aged before repair. Specimens were tested for SBS, and the failure type was observed with a magnifying loupe. Representative images were obtained using a scanning electronic microscope. Data were analyzed by two-way analysis of variance and Tukey post hoc tests (P = .05). Differences were detected among different surface treatments and among different composite resins with equal surface treatments (P < .05). SBS means ranged from 10.48 (FOR:G2) to 20.70 (FXT:G7). The highest SBS values were seen in G7 to G10 (P > .05), while lowest values were generally observed for G2. G1 showed higher results compared to G2 (P < .05), except for EDI (P > .05). Most failures corresponded with cohesive type. In general, thermally aged composite resin presented a decreased repair bond strength potential when no additional surface treatment was applied. Sandblasting improved the SBS of repaired aged composite resins.

In vitro analysis of durability of S-PRG filler-containing composite crowns for primary molar restoration.

OBJECTIVE: To evaluate the reliability, maximum principal stress, shear stress, and crack initiation of a computer-aided design/computer-aided manufacturing (CAD/CAM) resin composite (RC) incorporating surface pre-reacted glass (S-PRG) filler for primary molar teeth. METHODS: Mandibular primary molar crowns fabricated by experimental (EB) or commercially available CAD/CAM RCs (HC) were prepared and cemented to a resinous abutment tooth using an adhesive resin cement (Cem) or a conventional glass-ionomer cement (CX). These specimens were subjected to a single compressive test (n = 5/each) and the step-stress accelerated life testing (SSALT) (n = 12/each). Data was evaluated using Weibull analyses and reliability was calculated. Afterwards, the maximum principal stress and crack initiation point of each crown was analyzed by finite element analysis. To evaluate bonding of EB and HC to dentin, microtensile bond strength (µTBS) testing was conducted using primary molar teeth (n = 10/each). RESULTS: There was no significant difference between the fracture loads of EB and HC for either cement (p > 0.05). The fracture loads of EB-CX and HC-CX were significantly lower than EB-Cem and HC-Cem (p < 0.05). The reliability at 600 N for EB-Cem was greater than that for EB-CX, HC-Cem, and HC-CX. The maximum principal stress concentrated on EB was lower than that on HC. The shear stress concentrated in the cement layer for EB-CX was higher than that for HC-CX. There was no significant difference among the µTBSs of EB-Cem, EB-CX, HC-Cem, and HC-CX (p > 0.05). SIGNIFICANCE: The crowns fabricated with the experimental CAD/CAM RC incorporating S-PRG filler yielded greater fracture loads and reliability than the crowns manufactured with commercially available CAD/CAM RC regardless of the luting materials. These findings suggest that the experimental CAD/CAM RC crown may be clinically useful for the restoration of primary molars.