Study of the Vertical Structure of the Coastal Boundary Layer Integrating Surface Measurements and Ground-Based Remote Sensing.

The understanding of the atmospheric processes in coastal areas requires the availability of quality datasets describing the vertical and horizontal spatial structure of the Atmospheric Boundary Layer (ABL) on either side of the coastline. High-resolution Numerical Weather Prediction (NWP) models can provide this information and the main ingredients for good simulations are: an accurate description of the coastline and a correct subgrid process parametrization permitting coastline discontinuities to be caught. To provide an as comprehensive as possible dataset on Mediterranean coastal area, an intensive experimental campaign was realized at a near-shore Italian site, using optical and acoustic ground-based remote sensing and surface instruments, under different weather characteristic and stability conditions; the campaign is also fully simulated by a NWP model. Integrating information from instruments responding to different atmospheric properties allowed for an explanation of the development of various patterns in the vertical structure of the atmosphere. Wind LiDAR measurements provided information of the internal boundary layer from the value of maximum height reached by the wind profile; a height between 80 and 130 m is often detected as an interface between two different layers. The NWP model was able to simulate the vertical wind profiles and the eight of the ABL.

Exposure Assessment of Ambient PM2.5 Levels during a Sequence of Dust Episodes: A Case Study Coupling the WRF-Chem Model with GIS-Based Postprocessing.

A sequence of dust intrusions occurred from the Sahara Desert to the central Mediterranean in the second half of June 2021. This event was simulated by means of the Weather Research and Forecasting coupled with chemistry (WRF-Chem) regional chemical transport model (CTM). The population exposure to the dust surface PM2.5 was evaluated with the open-source quantum geographical information system (QGIS) by combining the output of the CTM with the resident population map of Italy. WRF-Chem analyses were compared with spaceborne aerosol observations derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) and, for the PM2.5 surface dust concentration, with the Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2) reanalysis. Considering the full-period (17-24 June) and area-averaged statistics, the WRF-Chem simulations showed a general underestimation for both the aerosol optical depth (AOD) and the PM2.5 surface dust concentration. The comparison of exposure classes calculated for Italy and its macro-regions showed that the dust sequence exposure varies with the location and entity of the resident population amount. The lowest exposure class (up to 5 µg m-3) had the highest percentage (38%) of the population of Italy and most of the population of north Italy, whereas more than a half of the population of central, south and insular Italy had been exposed to dust PM2.5 in the range of 15-25 µg m-3. The coupling of the WRF-Chem model with QGIS is a promising tool for the management of risks posed by extreme pollution and/or severe meteorological events. Specifically, the present methodology can also be applied for operational dust forecasting purposes, to deliver safety alarm messages to areas with the most exposed population.

Transcriptomics and Metabolomics Integration Reveals Redox-Dependent Metabolic Rewiring in Breast Cancer Cells.

Rewiring glucose metabolism toward aerobic glycolysis provides cancer cells with a rapid generation of pyruvate, ATP, and NADH, while pyruvate oxidation to lactate guarantees refueling of oxidized NAD+ to sustain glycolysis. CtPB2, an NADH-dependent transcriptional co-regulator, has been proposed to work as an NADH sensor, linking metabolism to epigenetic transcriptional reprogramming. By integrating metabolomics and transcriptomics in a triple-negative human breast cancer cell line, we show that genetic and pharmacological down-regulation of CtBP2 strongly reduces cell proliferation by modulating the redox balance, nucleotide synthesis, ROS generation, and scavenging. Our data highlight the critical role of NADH in controlling the oncogene-dependent crosstalk between metabolism and the epigenetically mediated transcriptional program that sustains energetic and anabolic demands in cancer cells.