Asunto(s)
Membrana Celular/análisis , Eritrocitos/análisis , Péptido Hidrolasas , Proteínas/análisis , Transporte Biológico , Carbohidratos/análisis , Inhibidores de la Colinesterasa , Detergentes , Compuestos de Diazonio , Difusión , Electroforesis Discontinua , Eritrocitos/enzimología , Glucosa/metabolismo , Humanos , Hidrólisis , Peso Molecular , Ácidos Neuramínicos/análisis , Ósmosis , Potasio/metabolismo , Isótopos de Azufre , TrometaminaRESUMEN
Parkinson's disease is a common neurodegenerative syndrome characterized by loss of dopaminergic neurons in the substantia nigra, formation of filamentous intraneuronal inclusions (Lewy bodies) and an extrapyramidal movement disorder. Mutations in the alpha-synuclein gene are linked to familial Parkinson's disease and alpha-synuclein accumulates in Lewy bodies and Lewy neurites. Here we express normal and mutant forms of alpha-synuclein in Drosophila and produce adult-onset loss of dopaminergic neurons, filamentous intraneuronal inclusions containing alpha-synuclein and locomotor dysfunction. Our Drosophila model thus recapitulates the essential features of the human disorder, and makes possible a powerful genetic approach to Parkinson's disease.