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1.
Cell ; 184(18): 4819-4837.e22, 2021 09 02.
Artículo en Inglés | MEDLINE | ID: mdl-34380046

RESUMEN

Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, cell types assemble into specific tissues and organs. To systematically explore the link between cell-type-specific gene expression and morphology, we registered an expression atlas to a whole-body electron microscopy volume of the nereid Platynereis dumerilii. Automated segmentation of cells and nuclei identifies major cell classes and establishes a link between gene activation, chromatin topography, and nuclear size. Clustering of segmented cells according to gene expression reveals spatially coherent tissues. In the brain, genetically defined groups of neurons match ganglionic nuclei with coherent projections. Besides interneurons, we uncover sensory-neurosecretory cells in the nereid mushroom bodies, which thus qualify as sensory organs. They furthermore resemble the vertebrate telencephalon by molecular anatomy. We provide an integrated browser as a Fiji plugin for remote exploration of all available multimodal datasets.


Asunto(s)
Forma de la Célula , Regulación de la Expresión Génica , Poliquetos/citología , Poliquetos/genética , Análisis de la Célula Individual , Animales , Núcleo Celular/metabolismo , Ganglios de Invertebrados/metabolismo , Perfilación de la Expresión Génica , Familia de Multigenes , Imagen Multimodal , Cuerpos Pedunculados/metabolismo , Poliquetos/ultraestructura
2.
Nucleic Acids Res ; 41(12): 6072-86, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23640331

RESUMEN

Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3'-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes.


Asunto(s)
ARN Polimerasa II/metabolismo , Receptores de Progesterona/metabolismo , Elongación de la Transcripción Genética , Proteína bcl-X/genética , Empalme Alternativo , Sitios de Unión , Proteína de Unión a CREB/metabolismo , Línea Celular Tumoral , Cromatina/química , Humanos , Factor B de Elongación Transcripcional Positiva/metabolismo , Promegestona/farmacología , Proteína bcl-X/biosíntesis , Proteína bcl-X/metabolismo , Factores de Transcripción p300-CBP/metabolismo
3.
Bioorg Med Chem ; 19(5): 1683-91, 2011 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-21315613

RESUMEN

The biological activity of two seven-membered A-ring (A-homo) analogues of progesterone was evaluated by transactivation assays in Cos-1 cells and by determination of Bcl-x(L) expression levels in T47D cells. The results show that both compounds act as selective progesterone receptor (PR) agonists but lack mineralocorticoid receptor (MR) activity. Molecular modelling using semiempirical AM1 and ab initio HF/6-31G** calculations, showed that the A-ring of the A-homo steroids may adopt five different conformations, although only three correspond to low energy conformers. The low energy conformers of each analogue were introduced into the ligand binding pocket of the PR ligand binding domain (LBD) obtained from the PR LBD-progesterone crystal structure. The steroid binding mode was then analyzed using 10 ns of molecular dynamics (MD) simulation. The PR LBD-progesterone complex was also simulated as a control system. The MD results showed that both A-homo steroids have one conformer that may be properly recognized by the PR, in agreement with the observed progestagen activity. Moreover, the simulation revealed the importance of a water molecule in the formation of a hydrogen bonding network among specific receptor residues and the steroid A-ring carbonyl.


Asunto(s)
Ligandos , Pregnanos/metabolismo , Progesterona/química , Receptores de Progesterona/metabolismo , Animales , Células COS , Chlorocebus aethiops , Modelos Moleculares , Simulación de Dinámica Molecular , Pregnanos/agonistas , Pregnanos/síntesis química , Progesterona/análogos & derivados , Progesterona/metabolismo , Receptores de Progesterona/agonistas
4.
Curr Top Dev Biol ; 141: 173-205, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33602488

RESUMEN

During the course of evolution, animals have become increasingly complex by the addition of novel cell types and regulatory mechanisms. A prime example is represented by the lateral neural border, known as the neural plate border in vertebrates, a region of the developing ectoderm where presumptive neural and non-neural tissue meet. This region has been intensively studied as the source of two important embryonic cell types unique to vertebrates-the neural crest and the ectodermal placodes-which contribute to diverse differentiated cell types including the peripheral nervous system, pigment cells, bone, and cartilage. How did these multipotent progenitors originate in animal evolution? What triggered the elaboration of the border during the course of chordate evolution? How is the lateral neural border patterned in various bilaterians and what is its fate? Here, we review and compare the development and fate of the lateral neural border in vertebrates and invertebrates and we speculate about its evolutionary origin. Taken together, the data suggest that the lateral neural border existed in bilaterian ancestors prior to the origin of vertebrates and became a developmental source of exquisite evolutionary change that frequently enabled the acquisition of new cell types.


Asunto(s)
Evolución Biológica , Invertebrados/embriología , Cresta Neural/citología , Vertebrados/embriología , Animales , Proteínas Morfogenéticas Óseas/metabolismo , Cordados no Vertebrados/embriología , Ectodermo/citología , Embrión no Mamífero/citología , Cresta Neural/metabolismo , Placa Neural/metabolismo
5.
Evodevo ; 12(1): 10, 2021 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-34579780

RESUMEN

The Nereid Platynereis dumerilii (Audouin and Milne Edwards (Annales des Sciences Naturelles 1:195-269, 1833) is a marine annelid that belongs to the Nereididae, a family of errant polychaete worms. The Nereid shows a pelago-benthic life cycle: as a general characteristic for the superphylum of Lophotrochozoa/Spiralia, it has spirally cleaving embryos developing into swimming trochophore larvae. The larvae then metamorphose into benthic worms living in self-spun tubes on macroalgae. Platynereis is used as a model for genetics, regeneration, reproduction biology, development, evolution, chronobiology, neurobiology, ecology, ecotoxicology, and most recently also for connectomics and single-cell genomics. Research on the Nereid started with studies on eye development and spiralian embryogenesis in the nineteenth and early twentieth centuries. Transitioning into the molecular era, Platynereis research focused on posterior growth and regeneration, neuroendocrinology, circadian and lunar cycles, fertilization, and oocyte maturation. Other work covered segmentation, photoreceptors and other sensory cells, nephridia, and population dynamics. Most recently, the unique advantages of the Nereid young worm for whole-body volume electron microscopy and single-cell sequencing became apparent, enabling the tracing of all neurons in its rope-ladder-like central nervous system, and the construction of multimodal cellular atlases. Here, we provide an overview of current topics and methodologies for P. dumerilii, with the aim of stimulating further interest into our unique model and expanding the active and vibrant Platynereis community.

6.
Sci Adv ; 4(2): eaao1261, 2018 02.
Artículo en Inglés | MEDLINE | ID: mdl-29492455

RESUMEN

Retinoic acid (RA) is an important intercellular signaling molecule in vertebrate development, with a well-established role in the regulation of hox genes during hindbrain patterning and in neurogenesis. However, the evolutionary origin of the RA signaling pathway remains elusive. To elucidate the evolution of the RA signaling system, we characterized RA metabolism and signaling in the marine annelid Platynereis dumerilii, a powerful model for evolution, development, and neurobiology. Binding assays and crystal structure analyses show that the annelid retinoic acid receptor (RAR) binds RA and activates transcription just as vertebrate RARs, yet with a different ligand-binding pocket and lower binding affinity, suggesting a permissive rather than instructive role of RA signaling. RAR knockdown and RA treatment of swimming annelid larvae further reveal that the RA signal is locally received in the medial neuroectoderm, where it controls neurogenesis and axon outgrowth, whereas the spatial colinear hox gene expression in the neuroectoderm remains unaffected. These findings suggest that one early role of the new RAR in bilaterian evolution was to control the spatially restricted onset of motor and interneuron differentiation in the developing ventral nerve cord and to indicate that the regulation of hox-controlled anterior-posterior patterning arose only at the base of the chordates, concomitant with a high-affinity RAR needed for the interpretation of a complex RA gradient.


Asunto(s)
Diferenciación Celular , Neuronas/citología , Neuronas/metabolismo , Filogenia , Receptores de Ácido Retinoico/metabolismo , Animales , Anélidos/efectos de los fármacos , Anélidos/embriología , Anélidos/genética , Anélidos/metabolismo , Axones/efectos de los fármacos , Axones/metabolismo , Diferenciación Celular/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Neuronas/efectos de los fármacos , Dominios Proteicos , Proteolisis/efectos de los fármacos , Receptores de Ácido Retinoico/química , Transducción de Señal/efectos de los fármacos , Tretinoina/metabolismo , Tretinoina/farmacología
7.
Endocrinology ; 151(12): 5730-40, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20881248

RESUMEN

The mammary epithelium undergoes cyclical periods of cellular proliferation, differentiation, and regression. During lactation, the signal transducer and activator of transcription factor (STAT)-5A and the glucocorticoid receptor (GR) synergize to induce milk protein expression and also act as survival factors. During involution, STAT3 activation mediates epithelial cell apoptosis and mammary gland remodeling. It has been shown that the administration of glucocorticoids at weaning prevents epithelial cell death, probably by extracellular matrix breakdown prevention. Our results show that the synthetic glucocorticoid dexamethasone (DEX) modulates STAT5A and STAT3 signaling and inhibits apoptosis induction in postlactating mouse mammary glands, only when administered within the first 48 h upon cessation of suckling. DEX administration right after weaning delayed STAT5A inactivation and degradation, preserving gene expression of target genes as ß-casein (bcas) and prolactin induced protein (pip). Weaning-triggered GR down-regulation is also delayed by the hormone treatment. Moreover, DEX administration delayed STAT3 activation and translocation into epithelial cells nuclei. In particular, DEX treatment impaired the increment in gene expression of signal transducer subunit gp130, normally up-regulated from lactation to involution and responsible for STAT3 activation. Therefore, the data shown herein indicate that glucocorticoids are able to modulate early involution by controlling the strong cross talk that GR, STAT5, and STAT3 pathways maintains in the mammary epithelium.


Asunto(s)
Dexametasona/farmacología , Glándulas Mamarias Animales/fisiología , Factor de Transcripción STAT3/metabolismo , Factor de Transcripción STAT5/metabolismo , Transducción de Señal/fisiología , Animales , Receptor gp130 de Citocinas/genética , Receptor gp130 de Citocinas/metabolismo , Fragmentación del ADN , Dexametasona/administración & dosificación , Femenino , Regulación de la Expresión Génica/fisiología , Glucocorticoides/administración & dosificación , Glucocorticoides/farmacología , Lactancia/fisiología , Factor Inhibidor de Leucemia/genética , Factor Inhibidor de Leucemia/metabolismo , Glándulas Mamarias Animales/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , Factor de Transcripción STAT3/genética , Factor de Transcripción STAT5/genética
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