RESUMEN
A rapid and accurate quantitative method was developed and validated for the analysis of four urinary organic acids with nitrogen containing functional groups, formiminoglutamic acid (FIGLU), pyroglutamic acid (PYRGLU), 5-hydroxyindoleacetic acid (5-HIAA), and 2-methylhippuric acid (2-METHIP) by liquid chromatography tandem mass spectrometry (LC/MS/MS). The chromatography was developed using a weak anion-exchange amino column that provided mixed-mode retention of the analytes. The elution gradient relied on changes in mobile phase pH over a concave gradient, without the use of counter-ions or concentrated salt buffers. A simple sample preparation was used, only requiring the dilution of urine prior to instrumental analysis. The method was validated based on linearity (r2>or=0.995), accuracy (85-115%), precision (C.V.<12%), sample preparation stability (Asunto(s)
Ácidos/orina
, Cromatografía por Intercambio Iónico/métodos
, Espectrometría de Masas en Tándem/métodos
, Ácidos/química
, Adolescente
, Adulto
, Anciano
, Anciano de 80 o más Años
, Resinas de Intercambio Aniónico
, Ácido Formiminoglutámico/química
, Ácido Formiminoglutámico/orina
, Hipuratos/química
, Hipuratos/orina
, Humanos
, Persona de Mediana Edad
, Estructura Molecular
, Compuestos Orgánicos/química
, Compuestos Orgánicos/orina
, Ácido Pirrolidona Carboxílico/química
, Ácido Pirrolidona Carboxílico/orina
, Reproducibilidad de los Resultados