Impact of Sulfur Starvation in Autotrophic and Heterotrophic Cultures of the Extremophilic Microalga Galdieria phlegrea (Cyanidiophyceae).

In plants and algae, sulfate assimilation and cysteine synthesis are regulated by sulfur (S) accessibility from the environment. This study reports the effects of S deprivation in autotrophic and heterotrophic cultures of Galdieria phlegrea (Cyanidiophyceae), a unicellular red alga isolated in the Solfatara crater located in Campi Flegrei (Naples, Italy), where H2S is the prevalent form of gaseous S in the fumarolic fluids and S is widespread in the soils near the fumaroles. This is the first report on the effects of S deprivation on a sulfurous microalga that is also able to grow heterotrophically in the dark. The removal of S from the culture medium of illuminated cells caused a decrease in the soluble protein content and a significant decrease in the intracellular levels of glutathione. Cells from heterotrophic cultures of G. phlegrea exhibited high levels of internal proteins and high glutathione content, which did not diminish during S starvation, but rather glutathione significantly increased. The activity of O-acetylserine(thiol)lyase (OASTL), the enzyme synthesizing cysteine, was enhanced under S deprivation in a time-dependent manner in autotrophic but not in heterotrophic cells. Analysis of the transcript abundance of the OASTL gene supports the OASTL activity increase in autotrophic cultures under S deprivation.

Sulfur Deprivation Results in Oxidative Perturbation in Chlorella sorokiniana (211/8k).

Sulfur deficiency in plant cells has not been considered as a potential abiotic factor that can induce oxidative stress. We studied the antioxidant defense system of Chlorella sorokiniana cultured under sulfur (S) deficiency, imposed for a maximum period of 24 h, to evaluate the effect of an S shortage on oxidative stress. S deprivation induced an immediate (30 min) but transient increase in the intracellular H2O2 content, which suggests that S limitation can lead to a temporary redox disturbance. After 24 h, S deficiency in Chlorella cells decreased the glutathione content to <10% of the value measured in cells that were not subjected to S deprivation. Consequently, we assumed that the cellular antioxidative mechanisms could be altered by a decrease in the total glutathione content. The total ascorbate pool increased within 2 h after the initiation of S depletion, and remained high until 6 h; however, ascorbate regeneration was inhibited under limited S conditions, indicated by a significant decrease in the ascorbate/dehydroascorbate (AsA/DHA) ratios. Furthermore, ascorbate peroxidase (APX) and superoxide dismutase (SOD) were activated under S deficiency, but we assumed that these enzymes were involved in maintaining the cellular H2O2 balance for at least 4 h after the initiation of S starvation. We concluded that S deprivation triggers redox changes and induces antioxidant enzyme activities in Chlorella cells. The accumulation of total ascorbate, changes in the reduced glutathione/oxidized glutathione (GSH/GSSG) ratios and an increase in the activity of SOD and APX enzymes indicate that oxidative perturbation occurs during S deprivation.

Antioxidant and anti-proliferative properties of extracts from heterotrophic cultures of Galdieria sulphuraria.

This study explores the possibility to use the extremophilic microalga Galdieria sulphuraria (strain 064) as a source of natural biomolecules with beneficial and protective effects on human health. Galdieria was cultivated in heterotrophy conditions and cells extracts for their antioxidant and anti-proliferative properties were tested. Galdieria extracts showed high antioxidant power tested through ABTS assay and revealed high glutathione and phycocyanin contents. Based on Annexin-V FITC/propidium iodide and MTT analysis, algae extracts inhibited the proliferation of human adenocarcinoma A549 cells (51.2% inhibition) through the induction of apoptosis without cell cycle arrest. Besides, cytotoxicity and cytometry assays showed a positive pro-apoptotic mechanism. On these bases, we suggest that G. sulphuraria from heterotrophic culture, for its therapeutic potential, could be considered a good candidate for further studies with the aim to isolate bioactive anti-cancer molecules.

Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea.

Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.