RESUMEN
Group B streptococci (Streptococcus agalactiae) cause a number of infections in women during pregnancy and postpartum, such as urinary tract infection, chorioamnionitis and endometritis, consequently may affect the newborn. Group B streptococci is the most common cause of severe infections in newborns in developed countries. Studies on the epidemiology of group B streptococci infections in Latin America are still limited. This information is also unknown in Mexico, but studies carried out in the center of the country have found high rates of vaginal colonization in pregnant women and there are case series and case reports of newborns. Microbiological and molecular epidemiology studies in Mexico have shown that populations of group B streptococci have a clonal distribution and that there are clones with genetic and phenotypic characteristics of high virulence that appear to be responsible for most of perinatal pathology. However, the actual role of group B streptococci in perinatal pathology in Mexico is unknown. Consequently, whether to perform or not the screening for determining the group B streptococci colonization status in pregnant women, and the indication or not for intrapartum antibiotic prophylaxis to prevent neonatal group B streptococci infection in Mexico, are still controversial.
Asunto(s)
Enfermedades del Recién Nacido/prevención & control , Transmisión Vertical de Enfermedad Infecciosa/prevención & control , Infecciones Estreptocócicas/epidemiología , Profilaxis Antibiótica/métodos , Femenino , Salud Global , Humanos , Recién Nacido , Enfermedades del Recién Nacido/microbiología , América Latina/epidemiología , Tamizaje Masivo/métodos , México/epidemiología , Embarazo , Complicaciones Infecciosas del Embarazo/diagnóstico , Complicaciones Infecciosas del Embarazo/microbiología , Infecciones Estreptocócicas/diagnóstico , Infecciones Estreptocócicas/prevención & control , Streptococcus agalactiae/aislamiento & purificaciónRESUMEN
OBJECTIVE: To determine whether prolonged exposure to antibiotics (>5 days) increases the risk of late-onset sepsis (LOS) in newborns of 1,000-1,500 g. METHODS: A cohort study in newborns with suspected perinatal infection, with a survival greater than seven days. The exposed cohort was composed of newborns with antibiotic therapy initiated at first postnatal day, lasting >5 days, with negative blood cultures before the fifth day of life, and without clinical evidence of sepsis. The non-exposed cohort was identical but with antibiotics stopped before the fifth day of life. Patients were followed daily for clinical and laboratory evidence of LOS. Others risk factors for LOS were analyzed. STATISTICAL ANALYSIS: We analyzed the incidence, the relative risk (RR) with 95% CI. To measure the time to occurrence of an event of LOS after exposure, Kaplan-Meier survival curve and log-rank test were used. RESULTS: We followed up 49 patients in each group. The incidence was 33.6%. The time of follow-up was 839 vs. 1,291 person-days. Prolonged exposure to antibiotics was associated with a higher risk of LOS (RR: 21.1; 95% CI: 6.5-68.9; p=0.000). The late-onset sepsis-free time was 17.1±1.1 vs. 26.3±0.8 days. CONCLUSIONS: The risk of LOS was higher in newborns with prolonged exposure to antibiotics and increased with the days of exposure.
Asunto(s)
Antibacterianos/administración & dosificación , Sepsis/epidemiología , Antibacterianos/efectos adversos , Estudios de Cohortes , Femenino , Estudios de Seguimiento , Humanos , Incidencia , Recién Nacido , Estimación de Kaplan-Meier , Masculino , Factores de Riesgo , Sepsis/etiología , Factores de TiempoRESUMEN
OBJECTIVE: The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction for detection of Brucella spp in human blood samples compared with the serological tests and blood culture. MATERIAL AND METHODS: In 2005, a total of 92 people were sampled from the towns of Anahuac and Sabinas Hidalgo, Nuevo Leon, where an outbreak of human cases had taken place in the same year as this study. The sera collected were analyzed by serological tests according to the NOM 022-SS2-1994. DNA was obtained using CTAB extraction method and it was used to amplify a fragment of 223 bp of the coding sequence for a protein of 31 kDa present in all Brucella species. RESULTS: The polymerase chain reaction test detected 23 positive samples. The sensitivity and specificity compared with RB was 44.68 and 95.56%, respectively. Compared with mouse antibody production, it was 51.61 and 88.52%, and 2-mercaptoethanol was 53.57 and 87.50%. When isolation (positives cultures) was compared with polymerase chain reaction, we obtained 100.0% sensitivity and 80.23% specificity, taking into account people with positive and negative serology. CONCLUSIONS: The polymerase chain reaction test can be an alternative tool to bacterial culture in human brucellosis diagnosis.
Asunto(s)
Sangre/microbiología , Brucella/aislamiento & purificación , Reacción en Cadena de la Polimerasa , Pruebas Serológicas , Humanos , Sensibilidad y EspecificidadRESUMEN
INTRODUCTION: Brucellosis poses a significant public health problem and requires meticulous diagnosis; the outcome has frequent relapses even when the treatment is appropriate. OBJECTIVE: To evaluate the response to the treatment in children with brucellosis by means of Huddleson seroaglutination test and PCR. METHODS: Using a prospective design, a cohort of children with brucellosis was followed up by carrying out Huddleson seroaglutination test of and PCR for Brucella at 6, 12 and 24 weeks. Most of children were treated with trimetoprim + sulfametoxazole and rifampicine. The progress towards therapeutic failure or relapse was evaluated. RESULTS: Twenty-three children fulfilled the inclusion criteria. The median age was 4.7 years; 61 % had consumed potentially infected milk or dairy products. The duration of symptoms ranged from 7 days to 1 year. Brucella sp. was isolated in blood culture in two of 21 children and Brucella melitensis in myeloculture in one of four children. 69 % had positive Huddleson serological test from 1:160 to >1:12000. PCR tested positive in 100% of children when entering to the study. Six weeks after beginning treatment 17% of children had therapeutic failure. At 12 weeks, three children (13 %) persisted with positive PCR and their antimicrobial treatment was modified. At 24 weeks, five children (21.7 %) presented relapse. A child persisted positive in spite of modifying the antimicrobial scheme. The agreement between the two tests was low in the three follow-up periods (k = 0.08, k = 0.12 and k = 0.28 respectively). CONCLUSIONS: A 6-week treatment cannot be enough to eliminate Brucella. PCR test can be used to early identify relapses.
Asunto(s)
Brucelosis/sangre , Brucelosis/tratamiento farmacológico , Adolescente , Niño , Preescolar , Femenino , Pruebas de Hemaglutinación , Humanos , Lactante , Masculino , Reacción en Cadena de la Polimerasa , Estudios Prospectivos , Resultado del TratamientoRESUMEN
BACKGROUND: Improper use of antibiotics increases antimicrobial resistance. OBJECTIVE: Evaluate the use of antibiotics and the impact of an intervention designed to improve antibiotic prescription for surgical prophylaxis in 6 hospitals of Monterrey, Mexico. MATERIAL AND METHODS: Design: A prospective multicenter survey and a pretest-postest experimental study. Phase 1: Survey to evaluate the use of antibiotics through an especially designed guide. Phase 2: Intervention designed to improve antibiotic prescription for surgical prophylaxis by the medical staff by using printed, audiovisual and electronic messages. Phase 3: Survey to evaluate the impact of the intervention. ANALYSIS: Frequencies, percentages, medians, ranges and X2 test. RESULTS: Phase 1: We evaluated 358 surgical patients, 274 prophylactic antibiotic regimens. A total of 96% of antibiotics regimens began with inappropriate timing (290/302), 82.8% were inappropriate regimens (274/331), 77.7% were in inappropriate dosage (230/296), 86% of inadequate length (241/280), and in 17.4% restricted antibiotics were used (52/299). Phase 2: 9 sessions including 189 physicians (14 department chairs, 58 general practitioners and 117 residents). Phase 3: We evaluated 303 surgical patients, 218 prophylactic antibiotics regimens. Inappropriate treatment commencement was reduced to 84.1% (180/214) (P<0.001), inappropriate regimens to 75.3% (162/215) (P=0.03), inappropriate dosages to 51.2% (110/215) (P<0.001), and use of restricted antibiotics to 8.3% (18/215) (P=0.003). CONCLUSIONS: Inappropriate use of prophylactic antibiotics in surgery is a frequent problem in Monterrey. The intervention improved the antibiotic prescription for surgical prophylaxis by reducing inappropriate treatment commencement, regimens, dosages, and overuse of restricted antibiotics. It is necessary to strengthen strategies to improve the prescription of antibiotics in surgical prophylaxis.
Asunto(s)
Profilaxis Antibiótica , Programas de Optimización del Uso de los Antimicrobianos/organización & administración , Prescripción Inadecuada/prevención & control , Pautas de la Práctica en Medicina/estadística & datos numéricos , Cuidados Preoperatorios , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antibacterianos/uso terapéutico , Niño , Preescolar , Prescripciones de Medicamentos/estadística & datos numéricos , Utilización de Medicamentos , Femenino , Hospitales Urbanos , Humanos , Prescripción Inadecuada/estadística & datos numéricos , Lactante , Recién Nacido , Masculino , México , Persona de Mediana Edad , Guías de Práctica Clínica como Asunto , Evaluación de Programas y Proyectos de Salud , Estudios Prospectivos , Infección de la Herida Quirúrgica/prevención & control , Adulto JovenRESUMEN
Although Structured Treatment Interruptions (STI) are currently not considered an alternative strategy for antiretroviral treatment, their true benefits and limitations have not been fully established. Some studies suggest the possibility of improving the quality of life of patients with this strategy; however, the information that has been obtained corresponds mostly to studies conducted in adults, with a lack of knowledge about its impact on children. Furthermore, mutations associated with antiretroviral resistance could be selected due to sub-therapeutic levels of HAART at each interruption period. Genotyping methods to determine the resistance profiles of the infecting viruses have become increasingly important for the management of patients under STI, thus low-abundance antiretroviral drug-resistant mutations (DRM's) at levels under limit of detection of conventional genotyping (<20% of quasispecies) could increase the risk of virologic failure. In this work, we analyzed the protease and reverse transcriptase regions of the pol gene by ultra-deep sequencing in pediatric patients under STI with the aim of determining the presence of high- and low-abundance DRM's in the viral rebounds generated by the STI. High-abundance mutations in protease and high- and low-abundance mutations in reverse transcriptase were detected but no one of these are directly associated with resistance to antiretroviral drugs. The results could suggest that the evaluated STI program is virologically safe, but strict and carefully planned studies, with greater numbers of patients and interruption/restart cycles, are still needed to evaluate the selection of DRM's during STI.
Asunto(s)
Fármacos Anti-VIH/uso terapéutico , Terapia Antirretroviral Altamente Activa , Farmacorresistencia Viral/genética , Infecciones por VIH/genética , Mutación , Adolescente , Femenino , Genotipo , Infecciones por VIH/tratamiento farmacológico , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Masculino , Carga ViralRESUMEN
OBJECTIVE: To identify microalbuminuria in patients with type 2 diabetes. MATERIAL AND METHODS: A descriptive cross-section study was made in patients with type 2 diabetes with more than a year of evolution. Microalbuminuria was defined as the presence of 30 to 300 mg of albumin in urine of 24 hours. The socio-demographic characteristics, type of diet, exercise, type of antihypertensive and oral antihyperglycemic agents were analyzed. Basal glycemia after one and two months, and renal state were assessed. RESULTS: Of 301 type 2 diabetic patients, 251 had microalbuminuria. The mean age was 57.2 years old. Women predominated over men (1.4:1). The mean for diabetes evolution was nine years. The complication with more frequency was hypertensive cardiopathy 78.1 %. A medium protein diet was present in 49%. 62.2% exercised and 40.2% exercised less than 30 minutes. Mean GFR with creatinine clearance was 83.3 +/- 32 mL/min. 98% of the cases received oral antihyperglycemic agents. Basal glycemia, after one and two months, was, on average, 171.8 mg/dL, 190.1 mg/dL, and 217.4 mg/dL. 74.5% of the patients had hyperglycemia in the first measuring, 79.3% in the second one, and 70.5% in the third one. CONCLUSIONS: The prevalence of microalbuminuria was greater than the reported in literature (85.3%). A poor control of glycemia was showed.
Asunto(s)
Albuminuria/etiología , Diabetes Mellitus Tipo 2/complicaciones , Nefropatías Diabéticas/etiología , Hipertensión/complicaciones , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Albuminuria/diagnóstico , Albuminuria/epidemiología , Glucemia/análisis , Presión Sanguínea , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/epidemiología , Nefropatías Diabéticas/diagnóstico , Nefropatías Diabéticas/epidemiología , Femenino , Humanos , Hipertensión/diagnóstico , Hipertensión/epidemiología , Hipoglucemiantes/uso terapéutico , Masculino , México/epidemiología , Persona de Mediana Edad , PrevalenciaRESUMEN
Se revisaron las caracteristicas farmacológicas del grupo de los imidazoles: ketoconazol, itraconazol y fluconazol y se compararon sus propiedades, interacciones, efectos secundarios e indicaciones. Se considera que este grupo de medicamentos dada su eficacia, seguridad y facilidad de administración, representan una buena alternativa en el manejo de las micosis profundas y sistémicas
Asunto(s)
Azoles/administración & dosificación , Triazoles/uso terapéutico , Ergosterol/antagonistas & inhibidores , Imidazoles/uso terapéutico , Micosis/terapiaRESUMEN
Objetivo: Determinar la eficacia de dos antibióticos (cefotaxima vs. cefuroxima) en la profilaxis de infecciones posquirúrgicas en cirugías limpias y limpias contaminadas en pacientes neuroquirúrgicos. Material y métodos: En el servicio de neurocirugía se realizaron 136 intervenciones quirúrgicas entre los meses de octubre a noviembre de 1994. Los criterios de inclusión fueron: ausencia de infección en otro órgano, cirugía limpia o limpia contamida, sin colocación de material sintético (DVP o craneoplastic). Los pacientes se dividieron en dos grupos: a los del grupo A se les dio cefotaxima y a los del B cefuroxima. Los antibióticos se prescribieron durante un periodo no mayor de 24 horas. La primera dosis de aplicó en el transcurso de la hora previa al inicio de la operación y cada cuatro horas durante la misma. La última dosis se administró cuatro horas después de terminada la intervención. La cefotaxima se aplicó a la dosis de un gramo cada vez y la cefuroxima a 750 mg por dosis. En los pacientes pediátricos la dosis se ajustó a 30 mg/kg de peso en ambos grupos. Resultados: Se incluyeron 54 pacientes, 28 del sexo masculino y 26 del femenino. El rango de edad fue de 3 a 75 años. Cada grupo estuvo conformado por 27 pacientes. Los procedimientos quirúrgicos fueron 33 craneotomías, nueve craniectomías suboccipitales, cinco transesfenoidales y siete espinales. El tiempo quirúrgico varió de 2 a 8 horas. El tiempo de utilización del microscopio fue, en promedio, de dos horas. Sólo hubo un caso de infección superficial en el grupo de la cefuroxima. No hubo diferencia estadísticamente significaiva entre los dos antibióticos utilizados (p exacta de Fisher = 0.5). conclusión: la incidencia de infecciones posquirúrgicas (limpias contaminadas) en neurocirugía es baja (3.7 por ciento). La profilaxis antibiótica puede usarse durante un tiempo menor de 24 horas. No hubo diferencia en la eficacia de los antibióticos utilizados, por lo que consideramos que es posible utilizar un antibiótico de menor costo y de mejor espectro contra gérmenes grampositivos (Staphylococcus aureus), como la cefuroxima, en el profilaxis de pacientes neuroquirúrgicos
Asunto(s)
Humanos , Masculino , Femenino , Lactante , Preescolar , Adolescente , Adulto , Persona de Mediana Edad , Cefalosporinas/uso terapéutico , Ensayo Clínico , Infección de la Herida Quirúrgica/tratamiento farmacológico , Infección de la Herida Quirúrgica/prevención & control , NeurocirugiaRESUMEN
Introducción. La profilaxis con antibióticos en cirugía cardiovascular disminuye la frecuencia de infecciones postoperatorias. Objetivo. Evaluar la eficacia y seguridad de tres esquemas de antibióticos empleados en nuestro hospital en cirugía cardiaca. Pacientes y métodos. Se realizó un estudio prospectivo, longitudinal, aleatorio y parcialmente controlado en 89 pacientes adultos sometidos a cirugía cardiovascular, entre marzo y agosto de 1995. Los pacientes se dividieron en tres grupos según el tratamiento recibido: A-cefuroxima (28 pacientes), B-cefalotina (32 pacientes) y C-dicloxacillina más amikacina (29 pacientes). Resultados. No hubo diferencias significativas entre los tres grupos en relación a la edad, sexo, factores de riesgo, duración de la cirugía, complicaciones no infecciosas (durante y después del procedimiento quirúrgico) y tasa de mortalidad. Las infecciones globales, respiratorias y de las heridas quirúrgicas tuvieron una tasa más alta en el grupo B. Los días de estancia de la UCI fueron más prolongados en los grupos B y C (p<0.05). Conclusión. La cefuroxima es efectiva en la prevención de la infecciones postoperatorias en cirugía cardiaca