The Need for Innovation in Rhinoplasty.

Rhinoplasty is a challenging surgery and results are not always perfect. There are many obstacles to achieving optimal results. Among these are inadequate instrumentation, the unpredictability of healing, imprecise planning, and many more. Furthermore, selecting patients who can most benefit from surgery is equally important. In this article, some of the more pressing areas of rhinoplasty that need innovation are discussed. From proper patient selection, to advances in education, to the standardization of training programs, to the development of sophisticated implants, the future of rhinoplasty surgery lies in continued creativity and innovation.

Lower Lateral Cartilages: An Anatomic and Morphological Study in Noses of Black Southern Africans.

Background: The anatomy of the nose of different ethnic groups has been widely researched in order to facilitate a better understanding of the individual nose as a foundation for improving surgical outcomes. The only anatomical research of the lower lateral cartilages (LLCs) available to the surgeon working with an African patient is to extrapolate data from studies already published on African Americans. Objectives: The aim of this descriptive cadaveric study was to assess the normal anatomy of the LLCs in noses of Black South Africans and compare this to data from studies on noses from Caucasian, Asian, Korean, and African-American populations. Methods: Ninety lower lateral cartilages of 45 cadavers of Black South Africans who did not have previous surgery or trauma to the nose were dissected. The morphological shapes and 12 standard anatomical measurements were recorded. The results were analyzed and compared to data in the literature from studies on lower lateral cartilages of Caucasian, Asian, Korean, and African-American populations. Results: A statistically significant difference was found in terms of overall cartilage dimensions, distance from nasal rim, and morphological shapes, compared to all previously studied groups, including the African-American population. There were significant differences in cartilage dimensions between males and females. This translates to clinically significant data that is useful during reconstructive and aesthetic nasal surgery on patients with a Southern African background. Conclusions: This study sets norms for alar cartilages in Black Southern Africans.

A polymorphism in the 5'-untranslated region of the porcine cholecystokinin type a receptor gene affects feed intake and growth.

The location and utilization of quantitative trait loci (QTL) and candidate genes with significant effects on economically important traits are becoming increasingly important in livestock breeding programs. The porcine cholecystokinin type A receptor (CCKAR) is a candidate gene for performance traits, due to its known role in the physiological control of feed intake, satiety, and obesity. We investigated the association of CCKAR polymorphisms with feeding, growth, and efficiency traits in an F2 population derived from a cross between Meishan and Large White founder animals and in lines of Large White pigs that had been divergently selected on the basis of lean growth efficiency traits. In the F2 population, CCKAR genotype was significantly associated with daily feed intake and average daily gain. The effects of the polymorphisms were then assessed in a larger-scale analysis of segregating commercial lines. A newly discovered single-nucleotide polymorphism (SNP) within the 5'-untranslated region (5'-UTR) had highly significant effects on feed intake, average daily gain, and days to 110 kg, which were not seen for a previously reported SNP within the CCKAR gene. Furthermore, we provide evidence that the novel SNP disrupts the binding of the YY1 transcription factor, which raises the possibility that it is the causal variant. The 5'-UTR SNP could be utilized as a molecular genetic test for increased feed intake, faster lean growth, and reduced days to market weight in segregating commercial lines.

Serum neuropeptide Y (NPY) and leptin concentrations in pigs selected for components of efficient lean growth.

Responses in log transformed serum neuropeptide Y (NPY) concentration and leptin concentration after six generations of divergent selection on components of efficient lean growth in pigs were measured. From an animal breeding perspective, serum NPY and/or leptin concentrations could be used as physiological predictors of genetic merit if there were significant responses to selection. At 90 kg liveweight, log transformed serum NPY concentrations were increased with divergent selection for low food conversion ratio (LFC) (6.31 versus 5.72, SED 0.09 log(pmol/L)) or for high lean growth rate (LGA) (5.80 versus 5.37 log(pmol/L)) but not with selection on daily food intake (DFI) (6.26 versus 6.14 log(pmol/L)). Selection for high DFI was associated with increased serum leptin concentration (3.06 versus 2.45, SED 0.21 ng/mL human equivalent (HE)) as was selection for low LFC (3.04 versus 2.46 ng/mL HE). Correlations between leptin and predicted lipid weight increased with stage of test (0.13, 0.34 and 0.43, SE 0.08 at 30, 50 and 75 kg). The high correlations between successive serum NPY concentrations (0.80, SE 0.11) suggest that changes in body composition with time would not be reflected in serum NPY concentrations. Serum NPY and, to a lesser extent, serum leptin concentrations were insensitive to dietary differences in total lysine: energy and indicated that studies using a genetic resource population of animals may be more powerful than nutritional studies using isoenergetic diets differing in lysine content to examine aspects of function of serum concentrations of NPY and leptin in pigs.

Serum urea concentration as a predictor of dietary lysine requirement in selected lines of pigs.

Serum urea concentrations were measured in Large White pigs from lines divergently selected for components of efficient lean growth rate and performance tested over three 14-d test periods starting at 30, 50, and 75 kg. Two methods of performance testing were used. Phase-fed pigs were fed to appetite isoenergetic diets differing in total lysine:energy ratio (0.58, 0.69, 0.81, 0.91, 1.01, 1.12, and 1.23 g/MJ of digestible energy), whereas diet-choice pigs were offered a choice of the 0.69 and 1.12 lysine:energy diets. Between test periods, all animals were fed one diet: 0.91 g of lysine/MJ of digestible energy. The study consisted of 230 boars and gilts with 150 pigs performance tested on phase-feeding and 80 pigs on diet-choice. The line selected for high lean food conversion had lower urea concentrations on each diet than the line selected for high lean growth rate, despite similar predicted lysine balances. Efficiency of lean growth rather than the rate of lean growth may be a better selection strategy in the context of nitrogen excretion. Urea concentrations at the end of each test period were correlated with lysine intake (0.33, 0.48 and 0.65; standard error, 0.08) and predicted lysine balance (0.39,0.44, and 0.64), but were uncorrelated with predicted lysine for protein deposition (0.01, 0.08, and 0.08) and maintenance. Urea concentration at the end of a test period was not a useful predictor of protein deposition, even after accounting for pretest variation in urea concentration and food intake during test. The expected response pattern of serum urea concentration to diets differing in total lysine:energy would be nonlinear, with the point of inflection occurring at the required dietary total lysine:energy for each genotype. However, there was no evidence of such an inflection point such that the prediction of lysine requirement from urea concentration was not possible for the selection lines in the study.

Fatty acid composition of lipid in Longissimus dorsi muscle of Duroc and British Landrace pigs and its relationship with eating quality.

The fatty acid composition of intramuscular fat from Duroc and halothane negative British Landrace pigs was determined and the relationships between fatty acid concentrations and aspects of eating quality were estimated from data on 160 pigs. Boars and gilts from each breed were performance tested on ad libitum or restricted feeding regimes with an average slaughter weight of 80 kg. Eating quality was assessed by ten experienced taste panelists. Intramuscular fat of Duroc pigs had higher concentrations of saturated and mono-unsaturated fatty acids and lower concentrations of poly-unsaturated fatty acids than Landrace pigs. With increasing intramuscular fat content, concentrations of saturated and mono-unsaturated fatty acids increased and concentrations of poly-unsaturated fatty acids decreased and the rate of change in fatty acid concentrations was greater in Landrace pigs than in Duroc pigs. Eating quality traits were generally improved as the concentration of mono-unsaturated fatty acids increased and poly-unsaturated fatty acids decreased.

Comparison of Duroc and British landrace pigs for meat a and eating quality.

Duroc and halothane negative British Landrace boars and gilts were performance tested from 30 to 80kg on ad-libitum or restricted feeding regimes, with like-sexed non-littermate groups of pigs penned together. Meat and eating quality was determined using objective laboratory measurements and by taste panel and consumer panel assessment on 160 pigs with 20 full-sib families for each breed, and two boars and two gilts per family. Duroc M. longissimus(†) was darker in colour, had a more intense, redder colour and contained more fat and less moisture than Landrace muscle. Duroc subcutaneous fat was less firm, had a higher water content and concentrations of linoleic acid and lower concentrations of stearic acid than Landrace fat. The taste and consumer panels scored Duroc meat as being more juicy, but less tender, having poorer flavour and being less acceptable than Landrace meat. The higher juiciness scores of Duroc meat were probably attributable to the higher intramuscular fat content compared to Landrace meat. Duroc boars had lower values for flavour liking and acceptability compared to other breed-sex combinations which may be due to the particular fatty acid composition of the subcutaneous fat in Duroc boars.

Boar taint in pigs selected for components of efficient lean growth rate.

Genetic and nutritional effects on the boar taint traits of androstenone, skatole and indole and the eating quality traits associated with boar taint were examined by testing animals from four selection lines and a control line on isoenergetic diets, which differed in ileal digestible lysine: digestible energy (0.40, 0.76 and 1.12 g lysine/MJ DE). The selected lines resulted from seven generations of selection for high daily food intake, lean food conversion ratio (LFC) and lean growth rate on ad libitum (LGA) or restricted (LGS) feeding regimes in a Large White population. During performance test, from 30 to 90 kg, boars were fed on either ad libitum or restricted (0.75 g/g ad libitum daily food intake) feeding regimes. A sensory panel assessed heated fat samples for androstenone odour, skatole odour and abnormal odour. There were no significant differences between the selection and control lines or diets for log transformed fat content of androstenone, skatole, indole. The significant diet with feeding regime interaction for log transformed fat content of skatole and indole were essentially due to significantly higher log transformed fat contents with ad libitum feeding of the high lysine diet compared to restricted feeding (skatole: -1.94 vs -3.06, s.e.d. 0.43; indole: -3.44 vs -4.22, s.e.d. 0.28), as differences between feeding regimes on diets A and C were not significantly different from zero. There were no significant differences between selection and control lines for sensory panel score for abnormal odour or androstenone odour, but the LFC and LGA selection lines had a significantly higher skatole odour score than the LGS selection line. Neither diet nor feeding regime had any significant effect on sensory panel assessment of odour. Log transformed fat content of androstenone and skatole were significantly correlated with sensory panel score for skatole odour (0.37 and 0.46, s.e. 0.12), but not with sensory panel score for androstenone odour (0.06 and 0.09), such that they would not be useful predictors of androstenone odour.

Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat.

The effects of genotype, diet and the genotype with diet interaction on fatty acid composition of neutral lipid and phospholipid of intramuscular fat in pigs were examined. Pigs from four selection lines and a control line were fed isoenergetic diets, which differed in ileal digestible lysine:digestible energy (A: 0.40, C: 0.76 and E: 1.12 g lysine/MJ DE). The selected lines resulted from seven generations of selection for high daily food intake (DFI), lean food conversion ratio (LFC) and lean growth rate on ad-libitum (LGA) or restricted feeding regimes in a Large White population. During performance test, from 30 to 90 kg, pigs were fed on either ad-libitum or restricted (0.75 g/g ad-libitum daily food intake) feeding regimes. A trained sensory panel assessed intensities of pork flavour and abnormal flavour and the hedonic characteristics of flavour liking and overall acceptability. The proportion of neutral lipid in the LGA line was lower (0.64 v 0.75, s.e.d. 0.03) while the polyunsaturated:saturated (P:S) ratio of the LFC line (0.53 v 0.41, s.e.d. 0.05) was higher than the control line and other selection lines. The DFI line had a higher phospholipid n-6:n-3 ratio than other selection lines (14 v 11, s.e.d. 1.0). Diets C and E resulted in lower neutral lipid:phospholipid ratio (0.7 v 0.8, s.e.d. 0.01), n-6:n-3 ratios for both neutral lipid (5 v 13, s.e.d. 0.8) and phospholipids (7 v 20, s.e.d. 0.6) and a higher P:S ratio (0.5 v 0.3, s.e.d. 0.03) for neutral lipid than diet A. Neutral lipid fatty acids C18:2 n-6, C18:3 n-3, C20:3 n-6, C20:4 n-6, C20:5 n-3, C22:5 n-3 and C22:6 n-3 were negatively correlated with pork flavour (-0.30, s.e.d. 0.10), flavour liking (-0.33) and overall acceptability (-0.30). In contrast, the fatty acids C16:1, C18:1 ω9 and C18:1 ω11 were positively correlated with pork flavour (0.36), flavour liking (0.39) and overall acceptability (0.40). However, correlations for fatty acids in the phospholipid class were positive for C18:2 n-6, C20:4 n-6 and C22:4 n-6 with pork flavour (0.33), flavour liking (0.23) and overall acceptability (0.23). The proportion of neutral lipid had non-significant correlations with flavour traits; pork flavour (0.01), abnormal flavour (0.08), flavour liking (-0.03) and overall acceptability (0.03). The selection lines responded in a similar manner to the different diets, such that there was little evidence for genotype with nutrition interactions for fatty acid concentrations of neutral lipids and phospholipids. Selection for high lean growth will reduce intramuscular fat, but the increased relative amount of phospholipid and concentration of polyunsaturated fatty acids are unlikely to reduce eating quality. Nutritional effects on intramuscular fat characteristics were greater than genetic effects, such that nutritional approaches to feeding pigs will provide effective methods of reducing the n-6:n-3 fatty acid ratio of human dietary fat from pigmeat and improving human health.

Sensitivity of pig genotypes to short-term manipulation of plasma fatty acids by feeding linseed.

The sensitivity of pigs selected for high daily food intake (DFI), low lean food conversion (LFC) and high lean growth rate (LGS) to dietary change of plasma fatty acids was assessed. The difference between the two diets was effectively a substitution of palmitic (C16:0), oleic (C18:1 n-9) and linoleic (C18:2 n-6) with linolenic (C18:3 n-3) fatty acids. Fatty acid compositions of plasma free fatty acids, neutral lipids and phospholipids were measured in 90 kg animals fed a base or high linolenic (C18:3 n-3) fatty acid diet, based on whole linseed, for four days. There were 24 animals from each selection line and 24 animals from an unselected control line, with boars and gilts represented equally in each line. Half of the selection line animals were fed the base diet and half were fed the high C18:3 diet, but all control animals were fed the base diet. Prior to slaughter, animals were fasted for 18 h. The fatty acids primarily affected by dietary change were C18:3 n-3 and its products, C20:5 n-3 (EPA) and C22:6 n-3 (DHA). The sensitivity of a selection line to dietary change was parameterised by the relative shift in fatty acid composition through changing from the base diet to the high C18:3 diet. In neutral lipids, the sensitivities of C18:3 n-3 in the DFI and LFC lines were similar but greater than in the LGS line (3.0 v. 1.8, S.E.D. 0.15), while, for phospholipid and free fatty acids, the sensitivity of the DFI line was greater than in the LFC and LGS lines (2.3 v. 1.8 and 2.0 v. 1.4, respectively). For C20:5 n-3 and C22:6 n-3, the DFI and LFC lines were more sensitive to dietary change than the LGS line (total lipid : 2.3 v. 1.9 and 1.5 v. 1.2). In general, the DFI line was most sensitive to dietary change and the LGS line was the least sensitive. The difference in sensitivities of the lipid classes to the high C18:3 diet between the selection lines could result from differences in body fat content and may explain the general lack of genotype with nutrition interactions in post-1990 genotypes in comparison with fatter circa-1970 genotypes.

Peri-partum posture and behaviour of gilts and the location of their piglets in lines selected for components of efficient lean growth.

Peri-partum posture and behaviour of gilts from lines selected for different components of efficient lean growth were studied to determine if behavioural changes may have been associated with the observed responses in reproductive performance. The proportions of time that gilts expressed defined posture and behaviour traits and the locations of their piglets were determined from video recordings of observations made at 5min intervals in the period extending from 2h pre-farrowing to 2h post-farrowing. The 137 gilts studied were from four pairs of Large White lines which had been divergently (high and low) selected for either daily food intake (DFI), lean food conversion efficiency (LFC), lean growth rate on ad libitum feeding (LGA) or lean growth rate on a restricted feeding scale (LGS).Almost all the significant (P<05) changes occurred in the LGS pair of lines. In the pre-farrowing period, relative to the low LGS gilts, high LGS gilts spent a higher proportion of their time lying on their sides (0.92 versus 0.69), and less time in the upright postures of standing, sitting or lying on their bellies (0.08 versus 0.33) and engaging in nesting behaviour (0.02 versus 0.10). During farrowing, high LGS gilts again lay on their sides more often than low LGS gilts (0.96 versus 0.80) and were upright less often (0.04 versus 0.20). High LGS gilts changed posture less often than low LGS gilts (0.05 versus 0.31) but were more often alert (0.79 versus 0.61). During farrowing, high LGS piglets were seen less often at their mother's head, back and vulva or at the creep than low LGS piglets (0.06 versus 0.15). Post-farrowing, there were no significant differences between the lines, almost all gilts lying on their sides with their piglets at the udder. Divergent selection for components of efficient lean growth rate on ad libitum feeding was not associated with consistent responses in gilt posture and behaviour or in piglet location. Selection for high lean growth on restricted feeding had effects on gilt posture and behaviour which may have been beneficial to her welfare and that of her piglets.

Design of multivariate selection experiments to estimate genetic parameters.

The precision of estimates of genetic variances and covariances obtained from multivariate selection experiments of various designs are discussed. The efficiencies of experimental designs are compared using criteria based on a confidence region of the estimated genetic parameters, with estimation using both responses and selection differentials and offspring-parent regression. A good selection criterion is shown to be to select individuals as parents using an index of the sums of squares and crossproducts of the phenotypic measurements. Formulae are given for the optimum selection proportion when the relative numbers of individuals in the parent and progeny generations are fixed or variable. Although the optimum depends on "a priori" knowledge of the genetic parameters to be estimated, the designs are very robust to poor estimates. For bivariate uncorrelated data, the variance of the estimated genetic parameters can be reduced by approximately 0.4 relative to designs of a more conventional nature when half of the individuals are selected on one trait and half on the other trait. There are larger reductions in variances if the traits are correlated.

Serum leptin concentration in pigs selected for high or low daily food intake.

Selection for high or low daily food intake (DFI) in Large White pigs resulted in higher serum leptin concentration, fat deposition and food intake in the high DFI line. The response in serum leptin concentration indicated that the higher fat deposition of the high DFI line was not due to insufficient leptin production, as in the Lepob/Lepob mouse. Serum leptin was more highly correlated with fat deposition than with food intake indicating that the response in serum leptin was primarily due to increased fat deposition rather than to higher energy intake per se. The low correlations between serum leptin measured at 30 kg and performance test traits indicate that serum leptin would not be efficient for selection of animals prior to performance test. However, the consistent positive correlations between serum leptin and a measure of fat deposition suggest that serum leptin could usefully be incorporated in selection criteria for genetic improvement of carcass lean content in pigs.

A melanocortin-4 receptor (MC4R) polymorphism is associated with performance traits in divergently selected Large White pig populations.

The melanocortin-4 receptor (MC4R) has a vital role in the control of energy balance and the genetic basis of obesity. A polymorphism, which results in the replacement of aspartic acid with asparagine at position 298 of the porcine MC4R gene, within the seventh transmembrane domain, has previously been described. In the current study, allele frequencies for this Asp298Asn polymorphism were investigated in lines of Large White pigs which had been divergently selected for seven generations based on lean food conversion (LFC), lean growth with ad libitum feeding (LGA), lean growth with restricted feeding (LGS) and daily feed intake (DFI). The association of the Asp298Asn polymorphism with performance traits in these lines was assessed. The frequency of Asp298 was higher (P < 0.001) in the LFC high line (0.48) than the low line (0.00), while the frequency of Asn298 was higher (P < 0.01) in the LGA high line (0.22) than the low line (0.04). When analysed across all lines, the Asp298Asn polymorphism was significantly associated with ultrasonic backfat depth, average daily gain and daily feed intake (P < 0.05). Asp298 homozygous animals had mean values of 13.3 mm, 733 g and 1933 g for backfat, average daily gain and daily feed intake respectively, compared with 14.7 mm, 805 g and 2098 g for Asn298 homozygotes. Therefore, the data support a role for the MC4R Asp298Asn polymorphism in the genetic basis of economically important traits in Large White pigs.

Discrimination between selected lines of pigs using AFLP markers.

Amplified fragment length polymorphic (AFLP) markers were used to discriminate between lines of pigs, divergently selected over seven generations for components of efficient lean growth rate. A total of 270 animals with 30 animals per line were genotyped for 239 polymorphic AFLP markers. Canonical variate analysis identified linear combinations of the AFLP marker scores that grouped animals by selection line with no overlap between selection lines. Cluster analysis of AFLP marker scores identified 10 groups of animals with 226 of the 270 animals clustered into nine groups, each consisting of animals from only one selection line. AFLP marker genotyping, using the EcoRI and TaqI restriction enzymes, provided an effective means of discriminating between animals of different selection lines that have arisen from one base population.