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1.
J Invest Surg ; 29(6): 335-342, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27050249

RESUMEN

PURPOSE: To investigate the potential protective effects of Proanthocyanidins(PAs) on intestinal motility disturbance following intestinal ischemia/reperfusion (I/R). MATERIALS AND METHODS: Male rats were divided into four groups: Sham, I/R, I/R+PA100 and I/R+PA200. Sham group underwent laparotomy without ligation, the others were subjected to intestinal ischemia for 1 h and reperfusion 4 h. Rats in the I/R+PA100 group received PAs (100 mg/kg/d) for 5 days prior to I/R, while rats in the I/R+PA200 group received PAs (200 mg/kg/d). After reperfusion, using an electrophysiology instrument measured ileal slow wave. Ileal specimens were obtained to determine contractility, tissue levels of Bax, Bcl-2, and Caspase-3 and evaluate histopathological changes. In addition, blood sample was obtained to determine serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels. RESULTS: Intestinal I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, and hemorrhage. Both PAs treatment decreased mucosal pathological impairment in comparison with the I/R group (p < .05) in light microscopic evaluations. In both PAs-treated groups, Bax and Caspase-3 expression were decreased compared to I/R group, while the Bcl-2 expression increased (p < .05), which was similarly the case for serum SOD activity demonstrated significant enhance (p < .05) and decline in MDA levels in comparison with I/R group (both p < .05). Moreover, PAs treatment was more efficient in attenuating serum MDA levels of intestinal I/R (both p < .05). And the contractile amplitude and frequency of slow wave in I/R+PA100 and I/R+PA200 groups were higher than I/R group (both p < .05). CONCLUSIONS: PAs improve intestinal motility disturbance following intestinal I/R by alleviating oxidative stress and apoptosis.


Asunto(s)
Motilidad Gastrointestinal/efectos de los fármacos , Íleon/efectos de los fármacos , Proantocianidinas/uso terapéutico , Daño por Reperfusión/tratamiento farmacológico , Animales , Caspasa 3/metabolismo , Evaluación Preclínica de Medicamentos , Íleon/irrigación sanguínea , Íleon/metabolismo , Íleon/patología , Masculino , Malondialdehído/sangre , Proantocianidinas/farmacología , Distribución Aleatoria , Ratas Sprague-Dawley , Daño por Reperfusión/sangre , Daño por Reperfusión/patología , Circulación Esplácnica , Superóxido Dismutasa/sangre , Proteína X Asociada a bcl-2/metabolismo
2.
World J Gastroenterol ; 19(5): 692-705, 2013 Feb 07.
Artículo en Inglés | MEDLINE | ID: mdl-23430052

RESUMEN

AIM: To investigate different methods of creating incomplete intestinal obstruction in a rat model and to compare their electrophysiologic, morphologic and histologic characteristics. METHODS: Rat ileum was partially obstructed by the respective application of: braided silk (penetrated the mesentery and surrounded intestine); half ligation (penetrated directly and ligated 1/2 cross-section of the intestine); wide pipe (6 mm in width, surrounded the intestine); narrow pipe (2 mm in width, surrounded the intestine). A control was also included (no obstruction). Various behavioral and electrophysiologic variables, as well as morphologic and immunohistochemical observations were recorded by blinded investigators at different time points (12, 24, 48, 72 h), including daily general condition, ileal wet weight and circumference, macromorphous and micromorphous intestine, bowel movement capability in vivo and in vitro, slow wave and neural electrical activity, and the number of c-Kit positive interstitial cells of Cajal (ICC). RESULTS: Despite being of a similar general condition, these methods resulted in different levels of obstruction in each group compared with the control at different time points (12, 24, 48, 72 h). However, these fields of the wide pipe rat showed significantly differences when compared with the other three obstructed groups at 12 to 72 h, including macroscopic and histological presentation, intestinal transit ratio and contractility, circumference and wet weight, amplitude and frequency of nerve electrical discharge and slow wave, and ICC numbers (all P < 0.01). CONCLUSION: The wide pipe rat method is significantly more reliable and stable than the other methods of obstruction, demonstrating that use of the wide pipe method can be a useful model of incomplete intestinal obstruction.


Asunto(s)
Enfermedades del Íleon/etiología , Íleon/cirugía , Obstrucción Intestinal/etiología , Animales , Biomarcadores/metabolismo , Modelos Animales de Enfermedad , Femenino , Tránsito Gastrointestinal , Enfermedades del Íleon/metabolismo , Enfermedades del Íleon/patología , Enfermedades del Íleon/fisiopatología , Íleon/inervación , Íleon/patología , Íleon/fisiopatología , Células Intersticiales de Cajal/metabolismo , Obstrucción Intestinal/metabolismo , Obstrucción Intestinal/patología , Obstrucción Intestinal/fisiopatología , Ligadura , Masculino , Complejo Mioeléctrico Migratorio , Proteínas Proto-Oncogénicas c-kit/metabolismo , Ratas , Ratas Sprague-Dawley , Factores de Tiempo
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