RESUMEN
The first complete mitochondrial genome for the Anostomidae fish family has been announced. Piracema fishes are the potamodromous migratory species from South America, which undergo lengthy and dramatic yearly reproductive upstream runs. The piracema species Leporinus piavussu has recently been described after a long misidentification history. Its mitogenome, assembled using NGS data and Sanger sequencing, consists in a 16,682 bp circular molecule (GenBank accession number KM886569). The exact sequence and position of 37 mitochondrial genes and the control region is established. A possible case of heteroplasmy was found with NGS and corroborated by Sanger sequencing. These results will positively contribute to the debate about this group's taxonomy, evolution and conservation.
Asunto(s)
Characiformes/genética , Genoma Mitocondrial , Animales , Codón , ADN Mitocondrial/química , ADN Mitocondrial/aislamiento & purificación , ADN Mitocondrial/metabolismo , Secuenciación de Nucleótidos de Alto Rendimiento , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p < 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78 percent), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31 percent) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08 percent). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a deleterious effect on meat quality traits and should be monitored in marker assisted selection programs.