RESUMEN
BACKGROUND: Epidermis hydration measurement is an important tool to assess its health. SkinUp® is a portable device that measures moisture and oil levels of skin through impedance method, it is cheap and has small dimensions, but this equipment has not yet been cited in the scientific literature. Thus, the objective of this work was to validate SkinUp® equipment by comparing its results with Corneometer® CM825. MATERIALS AND METHODS: Twenty subjects had skin hydration measurement on forearm, cheeks, and forehead. Measurements obtained with the two instruments were correlated by calculating the Pearson correlation coefficient and the P-value significance. The sensitivity of the equipment to different regions and treatments and their accuracy were also evaluated. RESULTS: Positive correlations between equipment were found for skin hydration, with P ≤ .001. Both instruments presented high sensitivity to the different treatments (P < .001). When sensitivity to different regions was evaluated, both showed greater hydration in the cheek and forehead when compared to the forearm (P < .005). The measurements between the cheek and forehead showed no significant difference when compared to each other. In addition, the two devices have good repeatability in the measurements. CONCLUSION: These results indicate that SkinUp® can be used in the future for skin analysis in in vivo assays.
Asunto(s)
Agua Corporal , Estado de Hidratación del Organismo , Piel , Belleza , Epidermis , Humanos , Fenómenos Fisiológicos de la PielRESUMEN
Ethanolic extracts of Achyrocline satureioides have pronounced antioxidant activity mainly due to the presence of the flavonoid quercetin. However, direct topical application of the extract is not possible due to the presence of high amounts of ethanol. In this sense, nanoemulsions arise as an alternative for topical formulation associating molecules with limited aqueous solubility. This article describes the development of topical nanoemulsions containing either A. satureioides extract or one of its most abundant flavonoid, quercetin. Nanoemulsions composed of octyldodecanol, egg lecithin, water and extract (NEE), or quercetin (NEQ) were prepared by spontaneous emulsification. This process led to monodisperse nanoemulsions presenting a mean droplet size of approximately 200-300 nm, negative zeta potential, and high association efficiency. A study of quercetin skin retention using porcine skin which was performed using a Franz diffusion cell revealed a higher accumulation of quercetin in skin for NEE when compared to NEQ. Finally, the antioxidant activity of formulations was measured by thiobarbituric acid-reactive species and the APPH model. A lower lipoperoxidation for the extract in respect to quercetin solution was observed. However, no difference between NEQ and NEE lipoperoxidation could be seen. The protection against lipoperoxidation by the formulations was also measured in the skin, where lower formation of reactive species was observed after treatment with NEE. In conclusion, this study shows the formulation effect on the physicochemical properties of nanoemulsions as well as on the skin retention and antioxidant activity of quercetin.
Asunto(s)
Achyrocline/química , Antioxidantes/farmacología , Asteraceae/química , Emulsiones/farmacología , Nanopartículas/administración & dosificación , Extractos Vegetales/farmacología , Animales , Antioxidantes/química , Química Farmacéutica/métodos , Emulsiones/química , Alcoholes Grasos/química , Flavonoides/química , Flavonoides/farmacología , Nanopartículas/química , Extractos Vegetales/química , Quercetina/química , Quercetina/farmacología , Piel/metabolismo , Absorción Cutánea/efectos de los fármacos , Solubilidad , PorcinosRESUMEN
AbstractDepending on the method of extraction, plant extracts can contain an enormous variety of active molecules, such as phenolic compounds, essential oils, alkaloids, among others. In many cases, from a pharmacological point of view, it is interesting to work with crude extract or fractions instead of a single isolated compound. This could be due to multi-targeting effect of the extract, lack of knowledge of the active compounds, synergistic effect of the extract compounds, among others. In any case, in order to achieve a final product some issues must be overcome, including poor stability, solvent toxicity, and low solubility of the bioactive compound. Recently many nanotechnology-based strategies have been proposed as an alternative to solve these problems, especially liposomes, nanoemulsions and nanoparticles. In this sense, the present work aims to review the main nanotechnological approaches used for association of different plant extracts and the main achievements from using these technologies.