Genomic and phenotypic features of Acinetobacter baumannii isolated from oil reservoirs reveal a novel subspecies specialized in degrading hazardous hydrocarbons.

The genus Acinetobacter encompasses biotechnologically relevant species and nosocomial pathogens. In this study, nine isolates recovered from different oil reservoir samples showed the ability to grow with petroleum as the only carbon source and possessed the ability to emulsify kerosene. The whole genomes of the nine strains were sequenced and analyzed. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of all strains were compared to the reference strains, and the results were below the reference values (<97.88 and 82, respectively), suggesting that the isolates belong to a new subspecies of Acinetobacter baumannii. The name Acinetobacter baumannii oleum ficedula is proposed. A comparison of the whole genome repertoire of 290 Acinetobacter species indicated that the strains in this study resemble non-pathogenic Acinetobacter strains. However, the new isolates resemble A. baumannii when comparing virulence factors. The isolates in this study carry many genes involved in hydrocarbon degradation, indicating the potential to degrade most toxic compounds listed by environmental regulatory agencies such as ATSDR, EPA, and CONAMA. In addition, despite the absence of known biosurfactant or bioemulsifier genes, the strains showed emulsifying activity, suggesting the presence of new pathways or genes related to this process. This study investigated the genomic, phenotypic, and biochemical features of the novel environmental subspecies A. baumannii oleum ficedula, revealing their potential to degrade hydrocarbons and to produce biosurfactants or bioemulsifiers. Applying these environmental subspecies in bioaugmentation strategies sheds light on future approaches to bioremediation. The study shows the importance of genomic analysis of environmental strains and their inclusion in metabolic pathways databases, highlighting unique enzymes/alternative pathways for consuming hazardous hydrocarbons.

Angioplastia transluminal coronária na fase intrahospitalar do infarto agudo do miocárdio / Angioplasty, Transluminal, percutaneous coronary

Os autores analisam a utilização da Angioplastia Transluminal Coronária, na fase intrahospitalar no infarto. Incluindo subgrupos de alto risco, mesmo nos pacientes tratados após as primeiras 12 horas. Mostram o estudo de 129 pacientes submetidos a ATC no IAM: 41 (32 'por cento') nas primeiras 6 h: 22 (17 'por cento') de 6 as 12 h e 66 (51 'por cento') após 12 horas. As Classes Funcionais (Killip), foram: I - 43 (33 'por cenro'); II - 26 (20 'por cento'); III - 26 (20 'por cento'); IV - 34 (27 'por cento'). O sucesso angigráfico nas 146 lesöes foi de 95 'por cento' de reperfusão, com 138 vasos dilatados. O sucesso clínico foi de 89 'por cento', (115 em 129), com onze óbitos (todos em CF III e IV). Houve três reoclusöes agudas, duas delas redilatadas com sucesso, foi submetido a revascularização cirúrgica devido a lesöes mjultiarteriais. Vinte e cinco pacientes foram tratados com ATC e implante de stent coronário. Concluem ser a ATC uma forma abrangente e segura de tratamento na fase intrahospitalar do IAM, principalmente nos subgrupos de alto risco, mesmo ultrapassadas as primeiras 6 horas.