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1.
Pediatr Allergy Immunol ; 35(6): e14165, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38822738

RESUMEN

Food allergies severely impact the health-related quality of life (HRQoL) of patients and their caregivers (family or informal caregivers). Currently there is no comprehensive review to provide an overview and critical assessment of the instruments in the field. Six databases were searched from inception until 10 August 2023, and a combination of subject terms and free words was used to search the literature. We used the COnsensus-based Standards for the selection of health Measurement INstruments methodology (COSMIN) to evaluate the measurement properties of the instruments. Forty-one studies reported on ten eligible instruments. Based on COSMIN guidelines, one instrument was recommended for Grade A, and the remaining nine instruments were recommended for Grade B. The Grade A instrument identified, the Food Allergy Quality of Life Questionnaire-Parent Form (FAQLQ-PF), can help researchers assess the effectiveness of treatment for patients with food allergy and to understand the psychosocial impact of the disease on patients.


Asunto(s)
Consenso , Hipersensibilidad a los Alimentos , Psicometría , Calidad de Vida , Humanos , Hipersensibilidad a los Alimentos/psicología , Hipersensibilidad a los Alimentos/diagnóstico , Psicometría/métodos , Encuestas y Cuestionarios , Cuidadores/psicología , Niño
2.
J Clin Nurs ; 33(7): 2427-2437, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38476038

RESUMEN

AIM: To investigate the factors that facilitate or hinder nurses in providing patient education. DESIGN: A mixed-method systematic review. DATA SOURCES: Six databases (Cochrane Library, PubMed, EMBASE, Web of Science, MEDLINE and ERIC) were systematically searched for relevant publications. METHODS: The study was conducted following the JBI for mixed-method systematic reviews, and the reporting followed the PRISMA guideline. Two researchers independently performed literature screening, literature evaluation, data extraction and synthesis. PROSPERO registration number: CRD42023427451. RESULTS: Twenty-six eligible articles were included, including 15 quantitative articles, 10 qualitative articles and 2 mixed-methods articles. The resultant synthesis of key findings led to the identification of these barriers and facilitators, categorised into five distinct levels: nurse-related factors, organisational factors, patient-related factors, the nurse-patient relationship and interdisciplinary collaboration. CONCLUSIONS: The findings highlight the factors that facilitate or hinder nurses in providing patient education, suggesting that multifaceted interventions can enhance the practice of patient education in nursing and support the development of appropriate patient education guidelines or public policies. RELEVANCE TO CLINICAL PRACTICE: This review delineates the facilitators and barriers influencing nurses' provision of patient education, offering an initial framework for nursing managers to craft interventions aimed at enhancing the quality of patient education provided by nurses, consequently elevating the overall quality of nursing.


Asunto(s)
Educación del Paciente como Asunto , Humanos , Educación del Paciente como Asunto/métodos , Relaciones Enfermero-Paciente , Femenino , Masculino , Adulto
3.
Artículo en Inglés | MEDLINE | ID: mdl-38867025

RESUMEN

The left-behind experience as an adverse childhood experience has a negative impact on the mental health of university students. Psychological inflexibility mediated the relationship between adverse childhood experiences and mental disorders, but no similar findings were drawn in psychological flexibility (PF). More research is needed to understand the relationship between PF and mental health of university students with left-behind experience. To investigate the relationship between PF profiles and mental health and sleep quality of university students with left-behind experience based on latent profile analysis. A sample of 1988 Chinese university students with left-behind experience were recruited to complete an online survey. Participants provided demographic information and completed validated measures of PF and mental health. Latent profile analysis was used to identify patterns of PF, and logistic regression analysis was used to examine the relationships among these variables. We found four PF profiles among participants, with the largest number being the moderately flexible profile (n = 808, 40.6%). The level of PF was positively correlated with mental health and sleep quality (all P < 0.001). Females, being left behind at a young age and for a long time, and having little contact with parents were associated with low PF (all P < 0.05). Our study highlights the importance of focusing on the PF of university students with left-behind experience and left-behind children, and the need for interventions to improve their PF and thus their mental health.

4.
BMC Nurs ; 23(1): 657, 2024 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-39278934

RESUMEN

AIM: To explore nurses' perceptions of evidence-based nursing courses for undergraduates through academic-practice partnerships. DESIGN: A deductive thematic analysis based on the practice-academic logic model. METHODS: Fifteen academic and clinical nurses were interviewed between November and December 2023, either online or through face-to-face meetings. Each interview lasted 20-30 min. The interview outline was constructed based on the practice-academic partnership logic model, which was followed during the process of recorded, analyzed, and checked. RESULTS: Themes identified include inputs (e.g., stakeholder commitment), activities (e.g., communication), outputs (e.g., nursing projects), and outcomes (e.g., improved competence). These themes highlight the various aspects and outcomes of academic-practice partnerships in evidence-based nursing courses. CONCLUSION: Effective academic-practice partnerships are crucial for developing evidence-based nursing courses, leading to positive educational and professional outcomes. IMPACT: Nurses' perceptions provide valuable guidelines for developing effective evidence-based nursing courses. PATIENT OR PUBLIC CONTRIBUTION: No patient or public users participated in this study.

5.
Int Nurs Rev ; 2024 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-38623890

RESUMEN

BACKGROUND: Academic-practice partnerships have the potential to solve many challenges in evidence-based nursing practice which is crucial for high-quality care. AIMS: To identify the existing knowledge on academic-practice partnerships in evidence-based nursing practice. METHODS: We conducted this review following the Joanna Briggs Institute scoping review methodology. We performed a comprehensive literature search of nine databases as well as five websites for gray literature. Two researchers independently conducted literature screening and data extraction and analysis. A third researcher was involved when needed. RESULTS: Dedicated time, dedicated resources, and compatible goals were found to be the top three inputs in academic-practice partnerships for evidence-based nursing practice. Meeting and discussion were the most popular forms of activities. Sufficient resources were the most important facilitators. Insufficient resources, insufficient time, and communication issues were the top three barriers. CONCLUSIONS: The assumption proposed in the practice-academic partnership logic model (i.e., inputs plus activities can lead to outputs and outcomes) was preliminarily verified by the results of this review in the context of evidence-based nursing practice. Academic-practice partnerships can leverage the advantages of both sides to overcome barriers and promote evidence-based nursing practice. However, it is essential to conduct a broader range of high-quality studies. Such endeavors could offer more comprehensive evidence for refining the framework of academic-practice partnerships in evidence-based nursing practice. IMPLICATIONS FOR NURSING EDUCATION, PRACTICE, POLICY AND RESEARCH: The "theoretical framework of academic-practice partnerships in evidence-based nursing practice" could theoretically guide academic and clinical nursing staff to collaborate on evidence-based nursing practice and related research and education programs. The academic-practice partnerships in evidence-based nursing practice could lead to a win-win situation for both the academic and the clinical sides. Furthermore, the results of this study provide an evidence-based foundation for policymakers to develop supportive policies for academic-practice partnerships in evidence-based nursing practice.

6.
J Gerontol Soc Work ; 67(4): 492-514, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38590208

RESUMEN

This pilot randomized controlled trial aimed to evaluate the feasibility and potential outcomes of an innovative 16-session multicomponent intervention model to improve cognitive abilities in older adults with mild cognitive impairment (MCI) by promoting healthy lifestyle, cognitive skills, tai chi and mindfulness practices. This study was a multicentre, randomized controlled, two-arm, parallel-group, unblinded trial in Hong Kong. 57 Chinese older adults with MCI recruited from three local elderly centers were randomly assigned to either the control or intervention group. The study results support the feasibility and efficacy of the multicomponent intervention, and recommend future larger-scale randomized control trials.


Asunto(s)
Disfunción Cognitiva , Humanos , Disfunción Cognitiva/terapia , Anciano , Masculino , Femenino , Proyectos Piloto , Hong Kong , Anciano de 80 o más Años , Taichi Chuan/métodos , Atención Plena/métodos , Cognición , Persona de Mediana Edad
7.
J Clin Nurs ; 32(13-14): 3695-3706, 2023 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35988036

RESUMEN

AIMS AND OBJECTIVES: To explore the evidence-based nursing practice (EBNP) competencies of clinical and academic nurses and their collaboration needs for supporting EBNP. BACKGROUND: Academic-practice partnerships have strong potential to overcome the key barriers to EBNP. However, there is little known about the collaboration needs of clinical and academic nurses for EBNP. DESIGN: A cross-sectional study. METHODS: We recruited clinical and academic nurses online during November 2021 to January 2022. Using a reliable and validated scale and adapted questionnaires, data were collected relating to demographic information, EBNP-related resources availability, EBNP competencies and EBNP collaboration needs. These data were described using descriptive statistical methods. The t test, χ2 test and Mann-Whitney U test were used to evaluate if the different responses between clinical and academic nurses were statistically significant. This study was reported following the STROBET checklist. RESULTS: Two 240 clinical nurses and 232 academic nurses submitted questionnaires. There was no difference in overall EBNP competence between clinical and academic nurses. However, clinical nurses reported lower levels of competence and stronger intentions to collaborate with academic nurses when searching for, appraising, and synthesising evidence. Academic nurses reported lower levels of competence and stronger intentions to collaborate with clinical nurses for disseminating and implementing evidence. CONCLUSION: Clinical and academic nurses both reported high needs for collaborating to overcome their perceived role limitations. Clinical and academic nurses have different strengths and limitations in EBNP. These role differences and intentions to collaborate for different dimensions of EBNP competence suggest that clinical and academic nursing roles could be complementary to each other, offering opportunities for synergistic collaborations to better support overall EBNP. RELEVANCE TO CLINICAL PRACTICE: Healthcare and academic institutions should promote academic-practice partnerships as opportunities to gain complementary expertise on different dimensions of EBNP, and to improve nurses' competencies and confidence in EBNP overall.


Asunto(s)
Enfermería Basada en la Evidencia , Enfermeras y Enfermeros , Humanos , Estudios Transversales , Rol de la Enfermera , China , Encuestas y Cuestionarios , Competencia Clínica
8.
BMC Nurs ; 22(1): 410, 2023 Oct 31.
Artículo en Inglés | MEDLINE | ID: mdl-37907869

RESUMEN

AIM: The aim of this scoping review was to evaluate and summarise the measurement properties of nursing research competence instruments and provide a summary overview of the use of nursing research competence instruments. BACKGROUND: Increasing nursing research competence instruments have been developed. However, a systematic review and evaluation of nursing research competence instruments is lacking. METHOD: This scoping review was conducted following the Joanna Briggs Institute updated methodology for scoping reviews and reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews checklist. Reviewers searched articles in Eight English databases and two Chinese databases between April 1st, 2022, and April 30th, 2022. An updated literature search was conducted between March 1st and March 4th, 2023. The literature screening and data extraction were conducted by two reviewers, independently. A third reviewer was involved when consensus was needed. The COnsensus-based Standards for the selection of health Measurement Instruments methodology was used to evaluate the methodological quality and measurement properties of the nursing research competence instruments. RESULTS: Ten studies involving eight nursing research competence instruments were included. None of the existing instruments have assessed all measurement properties. A total of 177 empirical studies have utilized a nursing research competence instrument with tested measurement properties. CONCLUSION: 'Self-evaluated Nursing Research Capacity of Questionnaire (refined)' was identified as the most appropriate nursing research competence instrument in existing instruments. However, reviewers need to conduct further measurement properties studies on the existing nursing research competence instruments. IMPLICATIONS FOR THE NURSING POLICY: This study could guide the selection of appropriate nursing research competence instruments which could help to evaluate the nursing research competence of nurses and inform the development of intervention plans to enhance nursing research competence.

9.
Aust Crit Care ; 36(5): 754-761, 2023 09.
Artículo en Inglés | MEDLINE | ID: mdl-36376190

RESUMEN

BACKGROUND: Nurses of all levels are expected to be competent in managing clinical deterioration. Given their limited experience and basic-level knowledge, there is a concern about junior nurses' clinical and patient management skills. However, junior nurses' abilities to recognise and respond to clinical deterioration have not been adequately explored because of the absence of a comprehensive tool. OBJECTIVES: The aim of this study was to develop a new self-assessment scale to assess the junior nurses' recognition and response abilities to clinical deterioration and to examine its reliability and validity. METHODS: Scale items were based on literature reviews and interviews. The preliminary scale was generated through two rounds of expert review. A panel of five experts evaluated content validity. After a pilot study, the questionnaire was distributed to 168 junior nurses via convenience sampling. Subsequent statistical analysis of results included construct validity, internal consistency, and test-retest reliability. RESULTS: Six factors were included, and 69.310% of the total variance was explained by the 25 items comprising the scale. The Cronbach's alpha coefficient was 0.905 (95% confidence interval [CI]: 0.812-0.979) for the overall scale and 0.655-0.838 for its subscales. The Guttman split-half reliability was 0.856 (95% CI: 0.806-0.894). The test-retest reliability of the scale was 0.878 (95% CI: 0.836-0.911). CONCLUSION: We developed a scale for measuring the abilities of junior nurses to recognise and respond to clinical deterioration and confirmed its reliability and validity. More experimental studies are needed to further evaluate this instrument.


Asunto(s)
Deterioro Clínico , Enfermeras y Enfermeros , Humanos , Reproducibilidad de los Resultados , Proyectos Piloto , Psicometría/métodos , Encuestas y Cuestionarios
10.
J Clin Nurs ; 31(5-6): 755-769, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-34219294

RESUMEN

AIMS AND OBJECTIVES: To evaluate the effects of a blended emergent research training programme for clinical nurses on research competence and critical thinking. BACKGROUND: With the development of the nursing discipline and evidence-based nursing practice, nursing research competence is increasingly important for clinical nurses. Current nursing research training programmes cannot meet the needs of clinical nurses. DESIGN: A quasi-experimental study. METHODS: A total of 104 clinical nurses from two tertiary hospitals participated in this study. The intervention group received a blended emergent research training programme for clinical nurses, and the control group received no intervention. Nursing research competence and critical thinking were measured at baseline, after the intervention and three months after the intervention. The satisfaction with the training of clinical nurses in the intervention group was also measured after the intervention. Generalised linear mixed models were used to evaluate the effects of the intervention on outcome variables. Qualitative data were analysed by two researchers using the content analysis method. Our reporting complies with the TREND checklist. RESULTS: The blended emergent research training programme for clinical nurses finally showed positive effects on nursing research competence and critical thinking. The effects lasted until three months after the training programme. 'Learner-centred Training', 'Skilled Trainers', 'Encouraging Training Environment' and 'Positive Outcomes' are the main categories generated from the qualitative data. CONCLUSION: The blended emergent research training programme for clinical nurses could be used for clinical nurses' research education to improve their nursing research competence and critical thinking. RELEVANCE TO CLINICAL PRACTICE: The blended emergent teaching method could be considered as an effective method to provide an encouraging learning environment and promote learner-centred teaching for clinical nurses. The blended emergent research training programme could be used to improve nursing research competence and critical thinking of clinical nurses which are important for evidence-based nursing practice.


Asunto(s)
Competencia Clínica , Investigación en Enfermería , Enfermería Basada en la Evidencia , Humanos , Aprendizaje , Pensamiento
11.
BMC Nurs ; 21(1): 2, 2022 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-34983524

RESUMEN

BACKGROUND: Nursing research training is important for improving the nursing research competencies of clinical nurses. Rigorous development of such training programs is crucial for ensuring the effectiveness of these research training programs. Therefore, the objectives of this study are: (1) to rigorously develop a blended emergent research training program for clinical nurses based on a needs assessment and related theoretical framework; and (2) to describe and discuss the uses and advantages of the ADDIE model (Analysis, Design, Development, Implementation, Evaluation) in the instructional design and potential benefits of the blended emergent teaching method. METHODS: This intervention development study was conducted in 2017, using a mixed-methods design. A theoretical framework of blended emergent teaching was constructed to provide theoretical guidance for the training program development. Nominal group technique was used to identify learners' common needs and priorities. The ADDIE model (Analysis, Design, Development, Implementation, Evaluation) was followed to develop the research training program for clinical nurses based on the limitations of current nursing research training programs, the needs of clinical nurses, and the theoretical foundation of blended emergent teaching. RESULTS: Following the ADDIE model, a blended emergent research training program for clinical nurses to improve nursing research competence was developed based on the needs of clinical nurses and the theoretical framework of blended emergent teaching. CONCLUSIONS: This study indicates that nominal group technique is an effective way to identify learners' common needs and priorities, and that the ADDIE model is a valuable process model to guide the development of a blended emergent training program. Blended emergent teaching is a promising methodology for improving trainees' learning initiative and educational outcomes. More empirical studies are needed to further evaluate blended emergent teaching to promote the development of related theories and practice in nursing education.

12.
BMC Nurs ; 21(1): 200, 2022 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-35879704

RESUMEN

BACKGROUND: Improving the professional identity of nursing intern is significant for enhancing the number of new registered nurses and easing the shortage of nursing personnel. The clinical internship is a key period for the formulation of professional identity. However, we know little about the factors influencing the nursing interns' professional identity during clinical internship. Therefore, this study explore the influencing factors of nursing interns' professional identity during clinical internship. This study will provide evidence and suggestions for generating effective strategies contributing to professional identity improvement of nursing interns. METHODS: This was a cross-sectional study. The convenience sampling was used to recruit 398 nursing interns from a teaching hospital in Hunan, China. The demographic characteristics information was collected by a self-developed questionnaire. The nursing interns' professional identity and potential influencing factors (e.g., work atmosphere, teacher capacity) were measured by questionnaires with good psychometric properties. The appropriate indicators were used for descriptive statistics, and t test, analysis of variance, Pearson's correlation analysis and multiple linear regression were used to analyse the influencing factors. RESULTS: In this study, the influencing factors of nursing interns' professional identity are education level, first choice of major, residential status, work atmosphere, and teacher capacity. The results showed that: (1) the nursing interns with a higher education level reported a lower level of professional identity; (2) the nursing interns whose first choice of major was not nursing discipline reported a lower level of professional identity; (3) the nursing interns live in rural areas (compared to urban areas) reported a higher level of professional identity; (4) the nursing interns in better work atmosphere reported a higher level of professional identity; (5) the nursing interns under the guidance of the teachers equipped with better teaching capacity reported a higher level of professional identity. CONCLUSION: The education level, first choice of major and residential status are influence factors of nursing interns' professional identity. The nursing educators need to pay attention to nursing interns whose first choice is not nursing, and in a bachelor program, who may have a lower level of professional identity. It is crucial to enhance the nursing interns' professional identity by improve the work atmosphere and clinical teachers' capacity, to promote nursing interns to choose nursing as a profession and reduce the shortage of nursing workforce.

13.
Res Soc Work Pract ; 32(8): 898-911, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-38603083

RESUMEN

Objective: This study aimed to test the feasibility and efficacy of a self-guided online cognitive behaviour therapy (iCBT) for university students in Hong Kong during COVID-19. Method: One group pre-post-test design with convenient sampling was adopted in this study, involving 84 university students who received a newly developed iCBT within an 8 week intervention period. The iCBT offered eight online modules for students to learn the skills of CBT at home through an online platform which was accessible any time anywhere anonymously with technical support only. Standardized assessment tools were used for outcomes assessment at the pre- and post-intervention periods. Results: Three quarters of participants completed all iCBT modules. The results of paired t-tests showed that, after completing the iCBT, participants showed reduction in anxiety, depression, and perceived stress. Conclusion: This study provides preliminary evidences to support the feasibility and efficacy of the self-guided iCBT for university students during COVID-19.

14.
J Clin Nurs ; 29(7-8): 1332-1340, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31971305

RESUMEN

AIMS AND OBJECTIVES: To explore the relationship between critical thinking disposition and research competence among clinical nurses. BACKGROUND: The development of the nursing discipline and evidence-based practice calls for research competence and critical thinking disposition among clinical nurses. The verification of the relationship between critical thinking disposition and research competence could make contributions to promoting related knowledge building and providing practical implications for nurses, nurse educators and nurse managers. However, there is a lack of evidence exploring the relationship between critical thinking disposition and research competence in clinical nurses. DESIGN: A cross-sectional study. METHODS: A total of 156 clinical nurses from two tertiary hospitals participated in this study. The Chinese Version of Critical Thinking Disposition Inventory and Research Competence Scale for Clinical Nurses were used to measure critical thinking disposition and nursing research competence, respectively. Data were collected in September 2017. Descriptive statistics, bivariate correlation and linear regression were used to analyse data. The STROBE checklist was used in reporting this study. RESULTS: The clinical nurses surveyed showed a positive inclination to general critical thinking but reported an overall low level of nursing research competence. A moderate degree of positive correlation was found between critical thinking disposition and research competence among clinical nurses. Educational degree was also found as an influencing factor of nursing research competence of clinical nurses. CONCLUSION: The critical thinking disposition of clinical nurses is positively related to their research competence. RELEVANCE TO CLINICAL PRACTICE: Nurses with a passion for nursing research should pay attention to improving their critical thinking dispositions. Nurse educators and managers should provide better learning, working and research environments and more supports to cultivate critical thinking disposition and improve nursing research competence in nursing research education and practice.


Asunto(s)
Investigación en Enfermería/normas , Pensamiento , Adulto , China , Estudios Transversales , Enfermería Basada en la Evidencia/métodos , Femenino , Humanos , Masculino , Investigación en Educación de Enfermería/organización & administración , Personal de Enfermería en Hospital/educación , Personal de Enfermería en Hospital/normas , Encuestas y Cuestionarios
15.
J Nanosci Nanotechnol ; 18(6): 4349-4354, 2018 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-29442786

RESUMEN

The wear resistance and stable mechanical properties affect the service life of the underwater functional materials to a certain extent. Unfortunately, the current study of underwater functional materials is rarely related to these aspects. Herein, we successfully designed and prepared polyurethane/graphite nanosheet (PU/GN) composite materials, which exhibited excellent wear resistance and stable mechanical properties underwater. The PU/GN composite films were prepared by evaporating a mixed solution of PU and GN on concave hexagonal honeycomb silicon templates. The mechanical properties of the composite films were determined by tensile test, and the wear resistance was evaluated by comparing the surface morphology before and after grind. By adjusting the content of graphite in the composite films, we found that the composite films containing 23 wt% GN had higher tensile strength and superior wear resistance. Moreover, this composite film showed an outstanding stability when expose to water. The impressive results along with simple preparation process made PU/GN composite films had potential applications in robust underwater functional materials.

16.
J Nanosci Nanotechnol ; 18(8): 5660-5667, 2018 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-29458622

RESUMEN

Titanium dioxide (TiO2) has been regarded as an efficient photocatalyst for degradation of environmental pollutants. However, recovery of TiO2 nanoparticles from suspension limits its practical application. Herein, we reported a novel highly transparent poly(vinyl alcohol)(PVA)/TiO2 photocatalytic film via in-situ growth and solution casting method. TiO2 nanoparticles with average size of 10 nm were uniformly dispersed in transparent PVA matrix. The photocatalytic performance was investigated by photodegradation of methyl orange (MO) aqueous solution under solar light irradiation. PVA/TiO2 photocatalytic film exhibited remarkably high photocatalytic activity and excellent recyclable properties during multi-cycle use. PVA not only acted as a transparent supports for TiO2, but also worked as an efficient holes scavenger. The hydroxyl groups on PVA chains played a key role in separation of photo-generated electrons and holes, thus increased the photodegradation rate of MO. This work gives an easy and reliable way for polymer/TiO2 nanocomposites in practical environmental applications.

17.
Nurse Educ Pract ; 80: 104117, 2024 Sep 02.
Artículo en Inglés | MEDLINE | ID: mdl-39236343

RESUMEN

AIM: To develop an evidence-based nursing practice course framework for undergraduate nursing students from a perspective of academic-practice partnerships. BACKGROUND: Academic-practice partnerships could play a crucial role in bridging theory-practice gap in evidence-based nursing education. However, there is a lack of evidence-based nursing practice course framework for undergraduate nursing students from a perspective of academic-practice partnerships. DESIGN: A Delphi study. METHODS: The Delphi study was conducted by literature analysis, qualitative interview and Delphi expert consultation to develop this course framework. 15 and 12 experts were involved in round one and round two of Delphi expert consultation, respectively. The consensus threshold of two-round Delphi expert consultation was described by expert's positive coefficient (≥ 50 %), positive coefficient (≥ 70 %), expert authority coefficient (≥ 0.7), coefficient of variation (<0.25) and full score frequency (>20 %). RESULTS: The final course framework includes learning objectives (17 items); teaching chapters of theoretical classes (five items); teaching contents (30 items), methods (12 items) and class hours (9 items) of theoretical classes; and the responsibilities of academic teachers (11 items) and clinical teachers (11 items) in practical classes. CONCLUSION: The course framework developed by this study could provide guidance for evidence-based nursing education of undergraduate nursing students. The effectiveness of this course framework should be verified through further experimental studies in the future.

18.
Bioelectrochemistry ; 160: 108797, 2024 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-39154628

RESUMEN

The residue of tobramycin, a broad spectrum antibiotic commonly used in animal husbandry, has evitable impact on human health, which may cause kidney damage, respiratory paralysis, neuromuscular blockade and cross-allergy in humans. Sensitive monitoring of tobramycin in animal-derived food products is therefore of great importance. Herein, a new aptamer electrochemical biosensor for sensing tobramycin with high sensitivity is demonstrated via exonuclease III (Exo III) and metal ion-dependent DNAzyme recycling and hybridization chain reaction (HCR) signal amplification cascades. Tobramycin analyte binds aptamer-containing hairpin probe to switch its conformation to expose the toehold sequence, which triggers Exo III-based catalytic digestion of the secondary hairpin to release many DNAzyme strands. The substrate hairpins immobilized on the Au electrode (AuE) are then cyclically cleaved by the DNAzymes to form ssDNAs, which further initiate HCR formation of lots of long methylene blue (MB)-tagged dsDNA polymers on the AuE. Subsequently electro-oxidation of these MB labels thus exhibit highly enhanced currents for sensing tobramycin within the 5-1000 nM concentration range with an impressive detection limit of 3.51 nM. Furthermore, this strategy has high selectivity for detecting tobramycin in milk and shows promising potential for detect other antibiotics for food safety monitoring.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Técnicas Electroquímicas , Límite de Detección , Leche , Tobramicina , Tobramicina/análisis , Aptámeros de Nucleótidos/química , Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Leche/química , Animales , ADN Catalítico/química , ADN Catalítico/metabolismo , Oro/química , Contaminación de Alimentos/análisis , Antibacterianos/análisis , Exodesoxirribonucleasas/metabolismo , Exodesoxirribonucleasas/química , Electrodos , Hibridación de Ácido Nucleico
19.
Anal Chim Acta ; 1315: 342816, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-38879214

RESUMEN

BACKGROUND: The monitoring of concentration variation of the newly developed growth differentiation factor 15 (GDF15) biomarker in human serum is of great significance for diagnosing cardiovascular diseases. Current methods for the detection of the GDF15 protein mainly are based on antibody-assisted immunoassays, which encounter the limitations in terms of sensitivity, complexity and costs. The development of simple and sensitive biosensors for GDF15 can therefore facilitate the diagnosis of cardiovascular diseases. RESULTS: A new bimetallic quasi-Cu/Co-MOF nanozyme with high catalytic performance for electrochemical reduction of H2O2 is synthesized via simple one-step precipitation and low-temperature calcination method. Such nanozymes are further employed as amplification tags and coupled with cyclic entropy-driven DNA signal enhancement strategies to construct ultrasensitive aptamer-based biosensor for detecting GDF15 in human serums. GDF15 molecules associate with two aptamers and release the ssDNA trigger sequences via target-binding induced displacement reaction. These ssDNAs subsequently initiate cyclic DNA-fueled strand displacement and catalytic hairpin assembly (CHA) reaction cascades for confining many quasi-Cu/Co-MOF nanozymes on sensor electrode, which yield drastically amplified H2O2 reduction current for detecting GDF15 down to 0.12 pg mL-1 with a dynamic range of 0.5 pg mL-1 to 20 ng mL-1. The electrochemical aptasensor also presents good reproducibility and selectivity and exhibits the capability to detect GDF15 in diluent serums. SIGNIFICANCE: Our aptamer-based GDF15 protein electrochemical assay clearly outperforms current existing antibody-based methods and the quasi-Cu/Co-MOF nanozyme/entropy-driven cascaded signal amplification means can be used as a universal strategy for sensitive monitoring of different biomolecular markers for diverse applications.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Cobalto , Cobre , Técnicas Electroquímicas , Factor 15 de Diferenciación de Crecimiento , Estructuras Metalorgánicas , Aptámeros de Nucleótidos/química , Factor 15 de Diferenciación de Crecimiento/sangre , Factor 15 de Diferenciación de Crecimiento/química , Cobre/química , Humanos , Estructuras Metalorgánicas/química , Cobalto/química , Técnicas Biosensibles/métodos , Entropía , Peróxido de Hidrógeno/química , Límite de Detección , Técnicas de Amplificación de Ácido Nucleico , ADN/química
20.
Biosens Bioelectron ; 261: 116473, 2024 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-38878701

RESUMEN

Sensitive monitoring of luteinizing hormone (LH), a glycoprotein that regulates the synthesis of regulatory steroid hormones, can facilitate the diagnosis of various reproductive diseases. In this work, a new and highly catalytic Sulfur-doped and bimetal-coordinated CoFe(CN)5NO (denoted as S-CoFe(CN)5NO) nanoparticles are synthesized. Such material is further used to construct high performance sensing interface and coupled with primer exchange reaction (PER) and hybridization chain reaction (HCR) amplification cascades for sensitive electrochemical aptamer-based LH assay. Target LH molecules bind aptamer sequences in DNA duplex probes to liberate ssDNA strands, which initiate subsequent PER/HCR amplification cascades for the capture of many ferrocene (Fc)-tagged DNAs on sensing interface. S-CoFe(CN)5NO subsequently leads to catalytic oxidation of these Fc tags for yielding substantially magnified currents for realizing ultrasensitive assay of LH with the detection limit of 0.69 pM in range from 5 pM to 10 nM. Owing to the high specificity of aptamer, such sensor has high selectivity and can achieve low levels of LH assay in diluted serum samples. With the successful demonstration for detecting trace LH, such sensor can be easily extended as a universal aptamer-based electrochemical sensing method for monitoring various target analytes in the biomedical and biological fields.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Técnicas Electroquímicas , Límite de Detección , Hormona Luteinizante , Aptámeros de Nucleótidos/química , Técnicas Biosensibles/métodos , Humanos , Técnicas Electroquímicas/métodos , Hormona Luteinizante/sangre , Hormona Luteinizante/química , Catálisis , Azufre/química , Nanopartículas del Metal/química , Cobalto/química , Hibridación de Ácido Nucleico , Nanopartículas/química , Compuestos Ferrosos/química
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