RESUMEN
As a key regulator of plant photosynthesis, water use efficiency and immunity, stomata are specialized cellular structures that adopt defined shapes. However, our knowledge about the genetic players of stomatal pore formation and stomatal morphogenesis remains limited. Forward genetic screening, positional cloning, confocal and electron microscopy, physiological and pharmacological assays were employed for isolation and characterization of mutants and genes. We identified a mutant, dsm1, with impaired cytokinesis and deformed stomata. DSM1 is highly expressed in guard mother cells and guard cells, and encodes COBRA-LIKE 7 (COBL7), a plant-specific glycosylphosphatidylinositol (GPI)-anchored protein. COBRA-LIKE 7 and its closest homologue, COBL8, are first enriched on the forming cell plates during cytokinesis, and then their subcellular distribution and abundance change are correlated with the progressive stages of stomatal pore formation. Both COBL7 and COBL8 possess an ability to bind cellulose. Perturbing the expression of COBL7 and COBL8 leads to a decrease in cellulose content and inhibition of stomatal pore development. Moreover, we found that COBL7, COBL8 and CSLD5 have synergistic effects on stomatal development and plant growth. Our findings reveal that COBL7 plays a predominant and functionally redundant role with COBL8 in stomatal formation through regulating cellulose deposition and ventral wall modification in Arabidopsis.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Celulosa/metabolismo , Regulación de la Expresión Génica de las Plantas , Fotosíntesis , Estomas de Plantas/metabolismoRESUMEN
Root hairs are cylindrical extensions of root epidermal cells that are important for the acquisition of water and minerals, interactions between plant and microbes. The deposition of cell wall materials in the tip enables root hairs to maintain elongation constantly. To date, our knowledge of the regulators that connect the architecture of cell wall and the root hair development remains very limited. Here, we demonstrated that COBL9 and COBL7, two genes of COBRA-Like family in Arabidopsis as well as their counterparts in rice, OsBC1L1 and OsBC1L8, regulate root hair growth. Single mutant cobl9, double mutants cobl7 cobl9 and double mutants osbc1l1 osbc1l8 all displayed prematurely terminated root hair elongation, though at varying levels. COBL7-YFP and COBL9-YFP accumulate prominently in the growing tips of newly emerged root hairs. Furthermore, cobl9, cobl7 cobl9 and osbc1l1 osbc1l8 mutants were defective in the enrichment of cellulose in the tips of the growing root hairs. We also discovered that overexpression of COBL9 could promote root hair elongation and salinity tolerance. Taken together, these results provide compelling evidence that the polarized COBL7 and COBL9 in the tip of the emerging root hairs have conserved roles in regulating root hair development and stress adaptation in dicots and monocots.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Celulosa/metabolismo , Meristema/metabolismo , Raíces de Plantas/metabolismo , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis/genética , Pared Celular/genética , Pared Celular/metabolismo , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Meristema/genética , Meristema/crecimiento & desarrollo , Microscopía Confocal , Mutación , Raíces de Plantas/genética , Raíces de Plantas/crecimiento & desarrollo , Plantas Modificadas Genéticamente , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Tolerancia a la Sal/genéticaRESUMEN
Silicon (Si) can increase salt tolerance of plants, and previous studies have focused on NaCl stress; whereas in protected facilities, nitrate (but not NaCl) accumulation is one of the major causes of secondary soil salinization. However, information on Si's effect on plant growth under nitrate stress is very limited, and the underlying mechanism is unknown. Here, we investigated Si's effect on plant growth, nitrogen assimilation and chlorophyll synthesis in cucumber. Cucumber seedlings ('Jinyou 1') were subjected to 200 mM nitrate stress without or with addition of 2 mM Si. The results showed that root application, but not foliar application of Si, could improve cucumber growth under nitrate stress. Root addition of Si increased photosynthetic rate and decreased oxidative damage of stressed plants. Under nitrate stress, Si addition decreased the accumulation of nitrate, nitrite and ammonium, and promoted the activities of nitrate reductase, nitrite reductase, glutamine synthase, glutamine-2-oxoglutarate aminotransferase and glutamate dehydrogenase in leaves. The concentrations of glutamic acid, 5-aminolevulinic acid, porphobilinogen and uroporphyrinogen â ¢ were increased under nitrate stress, while these were decreased by added Si. Added Si increased the levels of chlorophyll and its precursors (protoporphyrin â ¨, Mg-protoporphyrin â ¨ and protochlorophyllide), and expressions of genes encoding enzymes in chlorophyll synthesis (CHLH, POR and CAO) under nitrate stress. These results suggest that Si could improve cucumber growth under nitrate stress by enhancing nitrogen assimilation and chlorophyll synthesis, and imply an application of Si fertilizer in solving secondary soil salinization in protected facilities.
RESUMEN
A single agent that simultaneously inhibits multiple targets may offer greater therapeutic benefits in cancer than single-acting agents through interference with multiple pathways and potential synergistic action. In this work, a series of hybrids bearing N-phenylquinazolin-4-amine and hydroxamic acid moieties were designed and identified as dual VEGFR-2/HDAC inhibitors. Compound 6fd exhibited the most potent inhibitory activity against HDAC with IC50 of 2.2 nM and strong inhibitory effect against VEGFR-2 with IC50 of 74 nM. It also showed the most potent inhibitory activity against a human breast cancer cell line MCF-7 with IC50 of 0.85 µM. Docking simulation supported the initial pharmacophoric hypothesis and suggested a common mode of interaction at the active binding sites of VEGFR-2 and HDLP ((Histone Deacetylase-Like Protein), which demonstrates that compound 6fd is a potential agent for cancer therapy deserving further researching.