RESUMEN
We report here the genome sequence of the influenza A virus strain A/swine/Korea/61/2016, isolated from swine in the Republic of Korea. On the basis of sequence analysis, A/swine/Korea/61/2016 is marked from swine H1N1 influenza virus.
RESUMEN
The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the N and G genes, the Korean RABV isolates were confirmed as genotype I of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts.
Asunto(s)
Virus de la Rabia/genética , Rabia/veterinaria , Perros Mapache/virología , Animales , Secuencia de Bases , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/virología , China , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/virología , Perros , Glicoproteínas/genética , Datos de Secuencia Molecular , Nucleoproteínas/genética , Filogenia , Virus de la Rabia/clasificación , Virus de la Rabia/patogenicidad , República de Corea , Federación de Rusia , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
We analyzed the nucleotide sequences of the G-L (glycoprotein-large protein) intergenic non-coding region of 33 strains of the rabies virus (RABV) isolated in South Korea in 1998-2010 and compared the sequences with those of previously reported non-Korean strains. The similarities of the nucleotide sequences of the G-L region among all Korean RABV isolates ranged from 97.1 to 100%. Based on the phylogenetic analysis of the G-L region, the Korean RABV isolates were classified into three distinct subgroups with high similarity and were most closely related to the non-Korean NeiMeng1025C isolate, which was isolated from a rabid raccoon dog in eastern China, suggesting that the Korean RABV isolates originate from a rabid raccoon dog in northeastern Asia. Our results indicated that G-L region, as a useful phylogenetic indicator, is equivalent to the nucleoprotein (N) or glycoprotein (G) gene for study of RABV molecular epidemiology and that the Korean RABV isolates showing a few substitutions in the G-L region are continuously circulating in South Korea.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Enfermedades de los Perros/epidemiología , Virus de la Rabia/genética , Rabia/veterinaria , Animales , Bovinos , Enfermedades de los Bovinos/virología , ADN Viral/genética , ARN Polimerasas Dirigidas por ADN/genética , Enfermedades de los Perros/virología , Perros , Glicoproteínas/genética , Epidemiología Molecular , Filogenia , Rabia/epidemiología , Rabia/virología , Virus de la Rabia/aislamiento & purificación , República de Corea/epidemiología , Proteínas Virales/genéticaRESUMEN
Porcine epidemic diarrhea virus (PEDV) is a causative agent of severe diarrhea which leads to death in piglets. Because of the high mortality which is up to 100% in suckling piglets, PED is an important porcine disease in Korea. In this study, we developed a prophylactic candidate using single-chain Fvs to prevent the PEDV infection. ScFvs of mouse monoclonal antibody which was verified to neutralize PEDV was expressed in Escherichia coli expression system. After the confirmation of PEDV neutralizing activity of purified recombinant scFvs by VN test, scFvs were expressed on the surface of E. coli cells. The signal sequence and autotransporter beta domain of protease IgA (IgAP) of Neisseria gonorrhoeae were introduced to endow scFvs with the direction to the cell surface and the support as a transmembrane domain. 5x10(6)CFU of E. coli expressing scFvs against PEDV showed promising result of 94% foci reduction compared to wild type E. coli. This result demonstrated that E. coli expressing scFvs on the cell surface retained functional potency of parent antibody and therefore blocked PEDV infection into target cells in vitro. This in vitro assay result proposes the perspective of recombinant E. coli cells expressing scFvs as a novel prophylactic against PEDV infection.