Revolutionizing agriculture: Harnessing nano-innovations for sustainable farming and environmental preservation.

The agricultural sector is currently confronted with a significant crisis stemming from the rapid changes in climate patterns, declining soil fertility, insufficient availability of essential macro and micronutrients, excessive reliance on chemical fertilizers and pesticides, and the presence of heavy metals in soil. These numerous challenges pose a considerable threat to the agriculture industry. Furthermore, the exponential growth of the global population has led to a substantial increase in food consumption, further straining agricultural systems worldwide. Nanotechnology holds great promise in revolutionizing the food and agriculture industry, decreasing the harmful effects of agricultural practices on the environment, and improving productivity. Nanomaterials such as inorganic, lipid, and polymeric nanoparticles have been developed for increasing productivity due to their unique properties. Various strategies can enhance product quality, such as the use of nano-clays, nano zeolites, and hydrogel-based materials to regulate water absorption and release, effectively mitigating water scarcity. The production of nanoparticles can be achieved through various methods, each of which has its own unique benefits and limitations. Among these methods, chemical synthesis is widely favored due to the impact that various factors such as concentration, particle size, and shape have on product quality and efficiency. This review provides a detailed examination of the roles of nanotechnology and nanoparticles in sustainable agriculture, including their synthetic methods, and presents an analysis of their associated advantages and disadvantages. To date, there are serious concerns and awareness about healthy agriculture and the production of healthy products, therefore the development of nanotech-enabled devices that act as preventive and early warning systems to identify health issues, offering remedial measures is necessary.

Development of a microfluidic-assisted open-source 3D bioprinting system (MOS3S) for the engineering of hierarchical tissues.

The engineering of new 3D bioprinting approaches has shown great promise in the field of tissue engineering and disease modelling. However, the high cost of commercial 3D bioprinters has limited their accessibility, especially to those laboratories in resource-limited settings. Moreover, the need for a 3D bioprinting system capable of dispensing multiple materials is growing apace. Therefore, the development of a Microfluidic-assisted Open Source 3D bioprinting System (MOS3S) for the engineering of hierarchical tissues is needed to progress in fabricating functional tissues, but with a technology accessible to a wider range of researchers. The MOS3S platform is designed to allow the deposition of biomaterial inks using microfluidic printheads or coaxial nozzles for the in-situ crosslinking and scaffolds fabrication. The coupling of 3D printed syringe pumps with the motion control system is used for driving the tunable extrusion of inks for the fabrication of centimeter scale hierarchical lattice constructs for tissue engineering purposes. MOS3S performance have been validated to fabricate high-resolution structures with coaxial microfluidic technology, opening to new frontiers for seminal studies in pre-clinical disease modelling and tissue regeneration.

Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells.

Autograft or metal implants are routinely used in skeletal repair. However, they fail to provide long-term clinical resolution, necessitating a functional biomimetic tissue engineering alternative. The use of native human bone tissue for synthesizing a biomimetic material ink for three-dimensional (3D) bioprinting of skeletal tissue is an attractive strategy for tissue regeneration. Thus, human bone extracellular matrix (bone-ECM) offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells (HBMSCs) to proliferate and differentiate along the osteogenic lineage. In this study, we engineered a novel material ink (LAB) by blending human bone-ECM (B) with nanoclay (L, Laponite®) and alginate (A) polymers using extrusion-based deposition. The inclusion of the nanofiller and polymeric material increased the rheology, printability, and drug retention properties and, critically, the preservation of HBMSCs viability upon printing. The composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor (VEGF) enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane (CAM) model. The inclusion of bone morphogenetic protein-2 (BMP-2) with the HBMSCs further enhanced vascularization and mineralization after only seven days. This study demonstrates the synergistic combination of nanoclay with biomimetic materials (alginate and bone-ECM) to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair. Supplementary Information: The online version contains supplementary material available at 10.1007/s42242-023-00265-z.