RESUMEN
As important vectors of human disease, phlebotomine sand flies are of global significance to human health, transmitting several emerging and re-emerging infectious diseases. The most devastating of the sand fly transmitted infections are the leishmaniases, causing significant mortality and morbidity in both the Old and New World. Here we present the first global transcriptome analysis of the Old World vector of cutaneous leishmaniasis, Phlebotomus papatasi (Scopoli) and compare this transcriptome to that of the New World vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed using pooled mRNA from Phlebotomus papatasi larvae, pupae, adult males and females fed sugar, blood, or blood infected with Leishmania major. A total of 47 615 generated sequences was cleaned and assembled into 17 120 unique transcripts. Of the assembled sequences, 50% (8837 sequences) were classified using Gene Ontology (GO) terms. This collection of transcripts is comprehensive, as demonstrated by the high number of different GO categories. An in-depth analysis revealed 245 sequences with putative homology to proteins involved in blood and sugar digestion, immune response and peritrophic matrix formation. Twelve of the novel genes, including one trypsin, two peptidoglycan recognition proteins (PGRP) and nine chymotrypsins, have a higher expression level during larval stages. Two novel chymotrypsins and one novel PGRP are abundantly expressed upon blood feeding. This study will greatly improve the available genomic resources for P. papatasi and will provide essential information for annotation of the full genome.
Asunto(s)
Perfilación de la Expresión Génica , Proteínas de Insectos/genética , Phlebotomus/genética , Secuencia de Aminoácidos , Animales , Sangre/parasitología , Quimotripsina/genética , Quimotripsina/metabolismo , Etiquetas de Secuencia Expresada , Femenino , Biblioteca de Genes , Insectos Vectores/genética , Leishmania major , Masculino , Datos de Secuencia Molecular , Filogenia , Polimorfismo de Nucleótido Simple , Psychodidae/genética , Homología de Secuencia de Aminoácido , Tripsina/genética , Tripsina/metabolismoRESUMEN
Complex biological events occur during the developmental process of the mosquito Anopheles gambiae (Giles). Using cDNA expression microarrays, the expression patterns of 13,440 clones representing 8,664 unique transcripts were revealed from six different developmental stages: early larvae (late third instar/early fourth instar), late larvae (late fourth instar), early pupae (< 30 min after pupation), late pupae (after tanning), and adult female and male mosquitoes (24 h postemergence). After microarray analysis, 560 unique transcripts were identified to show at least a fourfold up- or down-regulation in at least one developmental stage. Based on the expression patterns, these gene products were clustered into 13 groups. In total, eight genes were analyzed by quantitative real-time polymerase chain reaction to validate microarray results. Among 560 unique transcripts, 446 contigs were assigned to respective genes from the An. gambiae genome. The expression patterns and annotations of the genes in the 13 groups are discussed in the context of development including metabolism, transport, protein synthesis and degradation, cellular processes, cellular communication, intra- or extra-cellular architecture maintenance, response to stress or immune-related defense, and spermatogenesis.
Asunto(s)
Anopheles/metabolismo , Animales , Anopheles/genética , Anopheles/crecimiento & desarrollo , Femenino , Perfilación de la Expresión Génica , Genes de Insecto , Larva/genética , Larva/metabolismo , Masculino , Anotación de Secuencia Molecular , Análisis de Secuencia por Matrices de Oligonucleótidos , Pupa/genética , Pupa/metabolismo , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Anopheles gambiae, the primary vector of human malaria in Africa, is responsible for approximately a million deaths per year, mostly of children. Despite its significance in disease transmission, this mosquito has not been studied extensively by genetic or molecular techniques. To facilitate studies on this vector, a genetic map has been developed that covers the X chromosome at an average resolution of 2 centimorgans. This map has been integrated with the chromosome banding pattern and used to localize a recessive, sex-linked mutation (white eye) to within 1 centimorgan of flanking markers.
Asunto(s)
Anopheles/genética , Mapeo Cromosómico , Genes de Insecto , Insectos Vectores/genética , Cromosoma X , Alelos , Animales , Secuencia de Bases , Bandeo Cromosómico , Cruzamientos Genéticos , ADN Satélite/genética , Femenino , Genes Recesivos , Marcadores Genéticos , Malaria/transmisión , Masculino , Datos de Secuencia Molecular , Mutación , Recombinación GenéticaRESUMEN
The severity of the malaria pandemic in the tropics is aggravated by the ongoing spread of parasite resistance to antimalarial drugs and mosquito resistance to insecticides. A strain of Anopheles gambiae, normally a major vector for human malaria in Africa, can encapsulate and kill the malaria parasites within a melanin-rich capsule in the mosquito midgut. Genetic mapping revealed one major and two minor quantitative trait loci (QTLs) for this encapsulation reaction. Understanding such antiparasite mechanisms in mosquitoes may lead to new strategies for malaria control.
Asunto(s)
Anopheles/genética , Anopheles/parasitología , Genes de Insecto , Insectos Vectores/genética , Insectos Vectores/parasitología , Plasmodium cynomolgi/inmunología , Animales , Anopheles/inmunología , Mapeo Cromosómico , Cruzamientos Genéticos , Femenino , Genotipo , Insectos Vectores/inmunología , Escala de Lod , Masculino , Melaninas/fisiología , Repeticiones de Microsatélite , FenotipoRESUMEN
Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.
Asunto(s)
Transportadoras de Casetes de Unión a ATP , Dípteros/genética , Proteínas de Drosophila , Proteínas del Ojo/genética , Genes de Insecto , Transformación Genética , Secuencia de Aminoácidos , Animales , Animales Modificados Genéticamente , Secuencia de Bases , Clonación Molecular , Dípteros/química , Drosophila melanogaster/genética , Color del Ojo/genética , Proteínas del Ojo/química , Marcadores Genéticos , Hormonas de Insectos/química , Hormonas de Insectos/genética , Datos de Secuencia Molecular , Mutación , Fenotipo , Alineación de SecuenciaRESUMEN
The anopheline mosquito is the target in most malaria control programs, primarily through the use of residual insecticides. A mosquito was studied that is refractory to most species of malaria through a genetically controlled mechanism. A strain of Anopheles gambiae, which was selected for complete refractoriness to the simian malaria parasite Plasmodium cynomolgi, also has varying degrees of refractoriness to most other malaria species examined, including the human parasites P. falciparum, P. ovale, and P. vivax for which this mosquito is the principal African vector. Furthermore, the refractoriness extends to other subhuman primate malarias, to rodent malaria, and to avian malaria. Refractoriness is manifested by encapsulation of the malaria ookinete after it completes its passage through the mosquito midgut, approximately 16 to 24 hours after ingestion of an infective blood meal. Fully encapsulated ookinetes show no abnormalities in parasite organelles, suggesting that refractoriness is due to an enhanced ability of the host to recognize the living parasite rather than to a passive encapsulation of a dead or dying parasite. Production of fully refractory and fully susceptible mosquito strains was achieved through a short series of selective breeding steps. This result indicates a relatively simple genetic basis for refractoriness. In addition to the value these strains may serve in general studies of insect immune mechanisms, this finding encourages consideration of genetic manipulation of natural vector populations as a malaria control strategy.
Asunto(s)
Anopheles/parasitología , Plasmodium/fisiología , Selección Genética , Animales , Anopheles/genética , Humanos , Insectos Vectores/parasitología , Malaria/parasitología , Malaria/prevención & control , Plasmodium falciparum/fisiología , Plasmodium vivax/fisiologíaRESUMEN
VectorBase, an integrated, relational database that manages genomic and other genetic/biological data pertaining to arthropod vectors of disease, has recently embarked on the construction of ontologies and controlled vocabularies (CVs). It aims, thus, at providing all necessary tools for the complete annotation of vector genomes and, in particular, the annotation of functional genomic data. This task was initiated with the development of anatomical ontologies of mosquitoes and ticks, both of which were made compliant to CARO, the common anatomy reference ontology. The ontologies are complemented by the development of novel web-based browsers that can show figures for anatomical terms, something that is especially helpful for fully illustrating the controlled vocabularies of anatomy.
Asunto(s)
Culicidae/anatomía & histología , Insectos Vectores/anatomía & histología , Garrapatas/anatomía & histología , Animales , Culicidae/genética , Bases de Datos Factuales , Bases de Datos Genéticas , Insectos Vectores/genética , Internet , Garrapatas/genéticaRESUMEN
Two distinct site-specific retrotransposon families, named RT1 and RT2, from the sibling mosquito species Anopheles gambiae and A. arabiensis, respectively, were previously identified. Both were shown to occupy identical nucleotide positions in the 28S rRNA gene and to be flanked by identical 17-bp target site duplications. Full-length representatives of each have been isolated from a single species, A. gambiae, and the nucleotide sequences have been analyzed. Beyond insertion specificity, RT1 and RT2 share several structural and sequence features which show them to be members of the LINE-like, or non-long-terminal-repeat retrotransposon, class of reverse transcriptase-encoding mobile elements. These features include two long overlapping open reading frames (ORFs), poly(A) tails, the absence of long terminal repeats, and heterogeneous 5' truncation of most copies. The first ORF of both elements, particularly ORF1 of RT1, is glutamine rich and contains long tracts of polyglutamine reminiscent of the opa repeat. Near the carboxy ends, three cysteine-histidine motifs occur in ORF1 and one occurs in ORF2. In addition, each ORF2 contains a region of sequence similarity to reverse transcriptases and integrases. Alignments of the protein sequences from RT1 and RT2 reveal 36% identity over the length of ORF1 and 60% identity over the length of ORF2, but the elements cannot be aligned in the 5' and 3' noncoding regions. Unlike that of RT2, the 5' noncoding region of RT1 contains 3.5 copies of a 500-bp subrepeat, followed by a poly(T) tract and two imperfect 55-bp subrepeats, the second spanning the beginning of ORF1. The pattern of distribution of these elements among five siblings species in the A. gambiae complex is nonuniform. RT1 is present in laboratory and wild A. gambiae, A. arabiensis, and A. melas but has not been detected in A. quadriannulatus or A. merus. RT2 has been detected in all available members of the A. gambiae complex except A. merus. Copy number fluctuates, even among the offspring of individual wild female A. gambiae mosquitoes. These findings reflect a complex evolutionary history balancing gain and loss of copies against the coexistence of two elements competing for a conserved target site in the same species for perhaps millions of years.
Asunto(s)
Anopheles/genética , Elementos Transponibles de ADN , ARN Ribosómico 28S/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
A test to evaluate constraints on the evolution of single microsatellite loci is described. The test assumes that microsatellite alleles that share the same flanking sequence constitute a series of alleles with a common descent that is distinct from alleles with a mutation in the flanking sequence. Thus two or more different series of alleles at a given locus represent the outcomes of different evolutionary processes. The higher rate of mutations within the repeat region (10(-3) or 10(-4)) compared with that of insertion/deletion or point mutations in adjacent flanking regions (10(-9)) or with that of recombination between the repeat and the point mutation (10(-6) for sequences 100 bp long) provides the rationale for this assumption. Using a two-phase, stepwise mutation model we simulated the evolution of a number of independent series of alleles and constructed the distributions of two similarity indices between pairs of these allele series. Applying this approach to empirical data from locus AG2H46 of Anopheles gambiae resulted in a significant excess of similarity between the main and the null series, indicating that constraints affect allele distribution in this locus. Practical considerations of the test are discussed.
Asunto(s)
Anopheles/genética , Simulación por Computador , Evolución Molecular , Repeticiones de Microsatélite , Modelos Genéticos , Alelos , AnimalesRESUMEN
We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitos from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1, 1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable markers, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.
Asunto(s)
Anopheles/genética , Mapeo Cromosómico , Malaria/parasitología , Animales , Marcadores Genéticos , HumanosRESUMEN
A Plasmodium-refractory strain of Anopheles gambiae melanotically encapsulates many species of Plasmodium, whereas wild-type mosquitoes are usually susceptible. This encapsulation trait can also be observed by studying the response of refractory and susceptible strains to intrathoracically injected CM-Sephadex beads. We report the results of broad-scale quantitative trait locus (QTL) mapping of the encapsulation trait using the bead model system. Interval mapping using the method of maximum likelihood identified one major QTL, Pen1. The 13.7-cM interval containing Pen1 was defined by marker AGH157 at 8E and AGH46 at 7A on 2R. Pen1 was associated with a maximum LOD score of 9.0 and accounted for 44% of the phenotypic variance in the distribution of phenotypes in the backcross. To test if this QTL is important for encapsulation of Plasmodium berghei, F2 progeny were infected with P. berghei and evaluated for degree of parasite encapsulation. For each of the two markers that define the interval containing Pen1, a significant difference of encapsulation was seen in progeny with at least one refractory allele in contrast with homozygous susceptible progeny. These results suggest that Pen1 is important for melanotic encapsulation of Plasmodium as well as beads.
Asunto(s)
Anopheles/genética , Insectos Vectores/genética , Melaninas/metabolismo , Animales , Anopheles/inmunología , Anopheles/parasitología , Mapeo Cromosómico , Dextranos , Ligamiento Genético , Marcadores Genéticos , Insectos Vectores/inmunología , MicroesferasRESUMEN
Anopheles gambiae and An. arabiensis are mosquito species responsible for most malaria transmission in sub-Saharan Africa. They are also closely related sibling species that share chromosomal and molecular polymorphisms as a consequence of incomplete lineage sorting or introgressive hybridization. To help resolve these processes, this study examined the partitioning of mtDNA sequence variation within and between species across Africa, from both population genetic and phylogeographic perspectives. Based on partial gene sequences from the cytochrome b, ND1 and ND5 genes, haplotype diversity was high but sequences were very closely related. Within species, little or no population subdivision was detected, and there was no evidence for isolation by distance. Between species, there were no fixed nucleotide differences, a high proportion of shared polymorphisms, and eight haplotypes in common over distances as great as 6000 km. Only one of 16 shared polymorphisms led to an amino acid difference, and there was no compelling evidence for nonneutral variation. Parsimony networks constructed of haplotypes from both species revealed no correspondence of haplotype with either geography or taxonomy. This trend of low intraspecific genetic divergence is consistent with evidence from allozyme and microsatellite data and is interpreted in terms of both extensive gene flow and recent range expansion from relatively large, stable populations. We argue that retention of ancestral polymorphisms is a plausible but insufficient explanation for low interspecific genetic divergence, and that extensive hybridization is a contributing factor.
Asunto(s)
Anopheles/genética , ADN Mitocondrial , Genes de Insecto , Variación Genética , Insectos Vectores/genética , Animales , Anopheles/clasificación , Femenino , Hibridación Genética , Insectos Vectores/clasificación , Malaria , MutaciónRESUMEN
The nucleotide (nt) sequence of a cDNA cloned from the mosquito Anopheles gambiae was determined. The amino acid (aa) sequence of the deduced protein was 56% identical (60/108 aa) to the recently discovered translation initiation factor Suil of yeast, suggesting that the two proteins are homologs and have similar functions. Database searches also revealed strong similarity to other sequences, including the deduced gene products of cDNAs from organisms as diverse as nematodes, humans and plants. The functions of these putative proteins are unknown, but their homology to Suil suggests that they represent an important component of the eukaryotic translation initiation complex.
Asunto(s)
Anopheles/genética , Proteínas Fúngicas/química , Factores de Iniciación de Péptidos/química , Proteínas de Saccharomyces cerevisiae , Secuencia de Aminoácidos , Animales , ADN Complementario , Factor 1 Eucariótico de Iniciación , Datos de Secuencia MolecularRESUMEN
New P element plasmids containing the organophosphate-degrading gene opd as a dominant selectable marker were tested as transformation vectors in Drosophila melanogaster. One of these vectors was modified by the addition of the D. melanogaster mini-white gene as a comarker. When transformed individuals were identified using paraoxon selection for opd alone, results were similar to those obtained with mini-white. No false positives were recovered, however one strain contained the mini-white gene but inadequate resistance to survive our screening regimen due to a defective Hsp70-opd gene. Results suggest that Hsp70-opd is similar to mini-white for distinguishing transformed individuals but does not require time-consuming individual examination. Due to the mode of action of organophosphorus nerve agents, Hsp70-opd has potential as a selectable marker in numerous animals beside fruit flies.
Asunto(s)
Drosophila melanogaster/genética , Marcadores Genéticos , Proteínas HSP70 de Choque Térmico/genética , Transformación Genética , Animales , Resistencia a los Insecticidas/genética , Insecticidas , Paraoxon , Monoéster Fosfórico Hidrolasas/genética , Monoéster Fosfórico Hidrolasas/metabolismo , PlásmidosRESUMEN
We report the identification of genomic sequences in various anopheline mosquitoes (family Culicidae: suborder Nematocera: order Diptera) showing homology to the class II, short inverted-terminal-repeat (ITR) transposable element P from Drosophila melanogaster (family Drosophilidae; suborder Brachycera: order Diptera). Anopheles gambiae appears to have at least six distinct P elements. Other anopheline species, including four additional members of the An. gambiae species complex (An. arabiensis, An. merus, An. melas and An. quadriannulatus), Anopheles stephensi (all subgenus Cellia), An. quadrimaculatus (subgenus Anopheles) and Anopheles albimanus (subgenus Nyssorhynchus) also possess P elements similar to those found in An. gambiae. Ten distinct P element types were identified in the genus Anopheles. At least two of the An. gambiae elements appears to be intact and potentially functional. Phylogenetic analysis of the anopheline P elements reveals them to belong to a distinctly different clade from the brachyceran P elements.
Asunto(s)
Anopheles/clasificación , Anopheles/genética , Genoma , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Secuencia Conservada , Cartilla de ADN , Elementos Transponibles de ADN/genética , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa/métodos , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
The Anopheles gambiae gene encoding tryptophan oxygenase, a homolog of the Drosophila melanogaster vermilion gene, has been molecularly cloned and characterized. Unlike Drosophila, where it is X-linked, the A. gambiae gene maps to chromosome 2R, subdivision 12E, by in situ hybridization to the polytene chromosomes. Of the six introns present, four are positioned identically to those of the Drosophila homolog, one is similarly positioned, and one is novel. A 1 955 nt cDNA potentially encodes a 392 amino acid protein of an estimated 45 kDa. Amino acid comparisons between the deduced protein and previously known tryptophan oxygenases revealed 74% identity between Anopheles and Drosophila, and 53% identity between Anopheles and nematode or mammalian proteins. Northern analysis detected a developmentally regulated transcript about 2 kb in length. Since this gene is known to control adult eye color in other flies, its cloning from A. gambiae provides the basis for a dominant phenotypic marker for germline transformation, one whose expression, unlike that of white, is not cell autonomous.
Asunto(s)
Anopheles/enzimología , Triptófano Oxigenasa/genética , Secuencia de Aminoácidos , Animales , Composición de Base , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , ADN Complementario , Drosophila , Humanos , Datos de Secuencia Molecular , Ratas , Homología de Secuencia de AminoácidoRESUMEN
The lack of eye pigment in the Aedes aegypti WE (white eye) colony was confirmed to be due to a mutation in the kynurenine hydroxylase gene, which catalyzes one of the steps in the metabolic synthesis of ommochrome eye pigments. Partial restoration of eye color (orange to red phenotype) in pupae and adults occurred in both sexes when first or second instar larvae were reared in water containing 3-hydroxykynurenine, the metabolic product of the enzyme kynurenine hydroxylase. No eye color restoration was observed when larvae were reared in water containing kynurenine sulfate, the precursor of 3-hydroxykynurenine in the ommochrome synthesis pathway. In addition, a plasmid clone containing the wild type Drosophila melanogaster gene encoding kynurenine hydroxylase, cinnabar (cn), was also able to complement the kynurenine hydroxylase mutation when it was injected into embryos of the A. aegypti WE strain. The ability to complement this A. aegypti mutant with the transiently expressed D. melanogaster cinnabar gene supports the value of this gene as a transformation reporter for use with A. aegypti WE and possibly other Diptera with null mutations in the kynurenine hydroxylase gene.
Asunto(s)
Aedes/genética , Drosophila melanogaster/genética , Oxigenasas de Función Mixta/genética , Animales , Clonación Molecular , Drosophila melanogaster/enzimología , Color del Ojo/genética , Regulación Enzimológica de la Expresión Génica/genética , Genes Reporteros/genética , Quinurenina 3-Monooxigenasa , Mutación/genética , Fenotiazinas/metabolismo , Pigmentos Biológicos/biosíntesisRESUMEN
We have cloned and sequenced members of a cuticular protein multi-gene family from the mosquito Anopheles gambiae. Three genes (agcp2a-c), each approximately 1 kb in length, were found in a 17.4 kb genomic phage clone. Analysis of ten cDNAs revealed that at least four related genes are present. The open reading frame of the genes and cDNAs showed 95% sequence identity. Divergence was observed in the sequence of the 3' ends and the number of copies of two repeated coding sequences. In situ hybridizations with a probe prepared from one of these circular protein genes physically mapped to two loci, 26B on chromosome 2L and 37A on 3R. Transcription of these An. gambiae cuticular protein genes appears to be limited to pharate pupae and the expressed protein(s) is found in early pupae. The deduced amino acid sequence of these proteins contains a hydrophilic region with significant similarity to other cuticular proteins including the pupal-specific cuticular protein, EDG84, of Drosophila melanogaster (Apple and Fristrom).
Asunto(s)
Anopheles/genética , Genes de Insecto , Proteínas de Insectos/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , ADN Complementario , Expresión Génica , Datos de Secuencia Molecular , Pupa , Conejos , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Factores de TiempoRESUMEN
Two outbreaks of Plasmodium vivax malaria have occurred recently in southern California, and Anopheles hermsi, a newly described species closely related to A freeborni, has been implicated as the vector. To assess the competence of A. hermsi as a vector, its susceptibility to P. vivax was compared with that of the efficient vector A. freeborni by allowing 150 females of each species to feed to repletion on infected squirrel monkeys. Oocyst density was greater in A. hermsi than in A. freeborni, and the frequency and density of sporozoites were similar. A. hermsi was susceptible to P. vivax and readily supported development to the sporozoite stage.
Asunto(s)
Anopheles/parasitología , Insectos Vectores/parasitología , Plasmodium vivax/crecimiento & desarrollo , Animales , California , Femenino , Malaria/transmisiónRESUMEN
In a genetically selected strain of the malaria vector Anopheles gambiae, most species of Plasmodium parasites are surrounded by melanized capsules and killed in the wall of the mosquito midgut. Genetic studies demonstrate a significant association between the refractory response to Plasmodium cynomolgi B strain and the Est A allele at an autosomal esterase locus. Increased susceptibility to parasites is associated with an alternate Est C allele. Lines selected to be homozygous for the Est A and Est C alleles show enhanced levels of refractoriness and susceptibility when compared to the unselected parental stock. Expression of the refractory phenotype is reduced much more than the susceptible phenotype at high parasite density, suggesting that refractoriness may be due to a positive effector.