RESUMEN
OBJECTIVE: To separate, purify total flavonoids from Cercis chinensis Bge and analyze their flavonoid aglycones and content by spectrum method. METHODS: The total flavonoids from Cercis chinensis Bge were extracted by 15 volume of 55% ethyl alcohol and hot water refluence at 100 degrees C and separated, purified by polyamide resin chromatography. Based on reference substances of rutin, quercetin, kaempferol, it was analyzed and detected by UV-Vis spectroscope at 200-600 nm and by KBr infrared spectroscope at 400-4000 cm(-1). RESULTS: Average extraction rate of crude total flavonoids was 7.32% , and extraction rate of purified total flavonoids was 2.20%. The results of UV-Vis and IR scanning spectrum showed that purified total flavonoids from Cercis chinensis Bge contained of 3 kinds of flavonoid aglycones of rutin, quercetin, kaempferol, and their content was 0.8271, 0.2169, 0.3007 mg/ml respectively. CONCLUSION: Content of total flavonoid aglycones in purified flavonoids of Cercis chinensis Bge was 24.30%, and content of total flavonoid was 0.5346%.