Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 30
Filtrar
Más filtros

Banco de datos
País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Cancer Causes Control ; 33(7): 951-958, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35415797

RESUMEN

PURPOSE: The incidence of colorectal cancer (CRC) in Ghana has increased eightfold since the 1960s. In 2011, national guidelines were set forth recommending all patients aged 50-70 years old undergo annual CRC screening with fecal occult blood testing (FOBT), but adherence to these guidelines is poor and screening rates remain low for unclear reasons. METHODS: We performed semi-structured interviews with 28 Ghanaians including physicians (n = 14) and patients (n = 14) from the Komfo Anokye Teaching Hospital in Kumasi, Ghana, to better understand the factors driving screening adherence and perceived barriers identified in an earlier quantitative study. RESULTS: Participants reported sociocultural factors such as reliance on alternative medicine or religion, lack of education, and financial burden as community-level barriers to CRC screening. At the system level, screening was limited by insufficient access to FOBT as well as a perceived lack of national prioritization. This was described as inadequate efforts from the Ministry of Health regarding national education as well as lack of incorporation of CRC screening into the National Health Insurance Scheme. CONCLUSION: Several community- and system-level barriers exist to widespread screening of CRC in Ghana. A multi-level approach will be required to improve rates of CRC screening and ultimately reduce the burden of CRC in Ghana.


Asunto(s)
Neoplasias Colorrectales , Médicos , Anciano , Neoplasias Colorrectales/diagnóstico , Neoplasias Colorrectales/epidemiología , Neoplasias Colorrectales/prevención & control , Detección Precoz del Cáncer , Ghana/epidemiología , Humanos , Tamizaje Masivo , Persona de Mediana Edad , Sangre Oculta
2.
BMC Cancer ; 17(1): 664, 2017 Oct 02.
Artículo en Inglés | MEDLINE | ID: mdl-28969611

RESUMEN

BACKGROUND: Over half of men who receive treatment for prostate suffer from a range of sexual problems that affect negatively their sexual health, sexual intimacy with their partners and their quality of life. In clinical practice, however, care for the sexual side effects of treatment is often suboptimal or unavailable. The goal of the current study is to test a web-based intervention to support the recovery of sexual intimacy of prostate cancer survivors and their partners after treatment. METHODS: The study team developed an interactive, web-based intervention, tailored to type of treatment received, relationship status (partnered/non-partnered) and sexual orientation. It consists of 10 modules, six follow the trajectory of the illness and four are theme based. They address sexual side effects, rehabilitation, psychological impacts and coaching for self-efficacy. Each includes a video to engage participants, psychoeducation and activities completed by participants on the web. Tailored strategies for identified concerns are sent by email after each module. Six of these modules will be tested in a randomized controlled trial and compared to usual care. Men with localized prostate cancer with partners will be recruited from five academic medical centers. These couples (N = 140) will be assessed prior to treatment, then 3 months and 6 months after treatment. The primary outcome will be the survivors' and partners' Global Satisfaction with Sex Life, assessed by a Patient Reported Outcome Measure Information Systems (PROMIS) measure. Secondary outcomes will include interest in sex, sexual activity, use of sexual aids, dyadic coping, knowledge about sexual recovery, grief about the loss of sexual function, and quality of life. The impact of the intervention on the couple will be assessed using the Actor-Partner Interaction Model, a mixed-effects linear regression model able to estimate both the association of partner characteristics with partner and patient outcomes and the association of patient characteristics with both outcomes. DISCUSSION: The web-based tool represents a novel approach to addressing the sexual health needs of prostate cancer survivors and their partners that-if found efficacious-will improve access to much needed specialty care in prostate cancer survivorship. TRIAL REGISTRATION: Clinicaltrials.gov registration # NCT02702453 , registered on March 3, 2016.


Asunto(s)
Neoplasias de la Próstata/epidemiología , Disfunciones Sexuales Fisiológicas/epidemiología , Disfunciones Sexuales Psicológicas/epidemiología , Estrés Psicológico , Adolescente , Adulto , Femenino , Humanos , Internet , Masculino , Persona de Mediana Edad , Neoplasias de la Próstata/complicaciones , Neoplasias de la Próstata/fisiopatología , Neoplasias de la Próstata/psicología , Calidad de Vida , Conducta Sexual/fisiología , Conducta Sexual/psicología , Disfunciones Sexuales Fisiológicas/etiología , Disfunciones Sexuales Fisiológicas/fisiopatología , Disfunciones Sexuales Psicológicas/etiología , Disfunciones Sexuales Psicológicas/fisiopatología , Parejas Sexuales , Esposos/psicología , Adulto Joven
3.
Urology ; 160: 161-167, 2022 02.
Artículo en Inglés | MEDLINE | ID: mdl-34896123

RESUMEN

OBJECTIVE: To evaluate patients' and partners' satisfaction with a prostate cancer survivorship program embedded in urologic-oncologic care. As a part of quality improvement activity, we developed a patient and partner-centered, biopsychosocial support program for men and partners coping with the urinary and sexual side-effects of surgical treatment for prostate cancer. The program became a part of usual care for all prostate cancer patients. METHODS: Patients who saw both an advanced practice provider and a sex therapist between August 1, 2018 and July 31, 2019 were eligible. Surveys packets were sent to 146 patients with surveys included for partners (N = 292). We used descriptive statistics to characterize participant responses. RESULTS: Responses were received from 88 patients and 70 partners (56% response rate for the group). Patients and partners reported very high or fairly high satisfaction with the rehabilitation activities of the program (86-97% and 90%-100%, respectively); 91% of patients and 84% of partners thought having pre-operative education and post-operative rehabilitation was a good or fairly good idea; 83% of patients and 79% of partners would very much or somewhat recommend the program to a friend who was considering surgical treatment for prostate cancer. CONCLUSION: Embedding a patient and partner-centered prostate cancer survivorship support program in oncologic care can positively impact patients' and partners' engagement in and satisfaction with post-operative rehabilitation.


Asunto(s)
Próstata , Neoplasias de la Próstata , Humanos , Masculino , Satisfacción del Paciente , Atención Dirigida al Paciente , Satisfacción Personal , Neoplasias de la Próstata/psicología , Neoplasias de la Próstata/cirugía , Parejas Sexuales/psicología , Supervivencia
4.
J Exp Med ; 167(4): 1313-22, 1988 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-3258624

RESUMEN

We have investigated the T cell populations in the cerebrospinal fluid (CSF) of chronic progressive multiple sclerosis (MS) patients. Individual T cells from the CSF and blood were cloned before expansion and their clonotypes were defined by analysis of rearranged T cell receptor beta chain and gamma chain genes. 87 T cell clones from blood and CSF of two patients with chronic progressive MS were examined for common TCR gene rearrangement patterns. In one patient, 18 of 28 CSF-derived T cell clones demonstrated common TCR gene rearrangements indicating oligoclonal T cell populations; in the blood, two patterns were found twice among 26 T cell clones. In another patient, 5 of 27 CSF-derived clones had common TCR gene rearrangement patterns. In contrast, no common beta chain rearrangement pattern was found among 67 T cell clones derived from the blood or CSF of a patient with subacute sclerosing panencephalitis, among 20 clones from the CSF of a patient with herpes zoster meningoencephalitis, or among 66 clones from a normal subject. A subject with atypical, fatal MS of 8-mo duration was also studied and did not have oligoclonal T cells in the CSF or blood. These results demonstrate that distinct oligoclonal T cell populations can be found in the CSF immune compartment of subjects with nonmalignant inflammatory disease and they can create a new avenue for the investigation of the specificity of the T cell response within the central nervous system.


Asunto(s)
Líquido Cefalorraquídeo/patología , Esclerosis Múltiple/líquido cefalorraquídeo , Linfocitos T/patología , Líquido Cefalorraquídeo/inmunología , Células Clonales/inmunología , Células Clonales/patología , Herpes Zóster/líquido cefalorraquídeo , Humanos , Meningoencefalitis/líquido cefalorraquídeo , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/patología , Receptores de Antígenos de Linfocitos T/genética , Panencefalitis Esclerosante Subaguda/líquido cefalorraquídeo , Linfocitos T/inmunología
5.
J Exp Med ; 165(5): 1453-8, 1987 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-3106557

RESUMEN

We have derived 33 independent T cell clones from the cerebrospinal fluid (CSF) of a patient with subacute sclerosing panencephalitis using a single T cell cloning method. 6% (2 of 33) of these clones express the T cell receptor gamma (TCR-gamma) protein and are called CSF TCR-gamma clones. Phenotypic analyses of the CSF TCR-gamma clones indicate that they are WT-31-, CD3+, CD4-, and CD8-. The TCR-gamma protein exists on the cell surface as part of an 85-kD disulphide-linked dimer noncovalently associated with the CD3 polypeptides. The CSF TCR-gamma clones have NK-like activity that can be inhibited by anti-CD3 mAbs. Both CSF TCR-gamma clones proliferated in response to anti-CD3 mAbs coupled to Sepharose beads and/or IL-2. Furthermore, stimulation of one of these clones with anti-CD3 mAbs results in a rapid rise in intracellular calcium. These data suggest that T cells bearing the CD3-TCR-gamma protein complex are functional and play a role in the human immune response.


Asunto(s)
Líquido Cefalorraquídeo/citología , Células Asesinas Naturales/ultraestructura , Receptores de Antígenos de Linfocitos T/inmunología , Células Clonales , Humanos , Cadenas gamma de Inmunoglobulina/metabolismo , Panencefalitis Esclerosante Subaguda/líquido cefalorraquídeo
6.
Science ; 228(4704): 1204-6, 1985 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-3839095

RESUMEN

The T-cell antigen receptor is a cell surface molecule vital in mediating the cellular immune response. The arrangement and rearrangement of the gene segments encoding the beta-chain polypeptide of the receptor are similar to those of immunoglobulin gene segments. The two constant region genes of the human T-cell antigen receptor are 8 kilobases apart with a cluster of joining segments located 5' of each constant region gene. Although most beta-chain gene rearrangements involve the variable, diversity, and joining segments, analysis of a beta-chain complementary DNA clone suggests the occasional occurrence of another type of rearrangement.


Asunto(s)
Receptores de Antígenos de Linfocitos T/genética , Secuencia de Bases , ADN/genética , Genes , Humanos , Sustancias Macromoleculares , Recombinación Genética
7.
Science ; 231(4735): 252-5, 1986 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-3079918

RESUMEN

The human T-cell gamma chain genes have been characterized in an attempt to better understand their role in immune response. These immunoglobulin-like genes are encoded in the genome in variable, joining, and constant segments. The human gamma genes include at least six variable region genes, two joining segments, and two constant-region genes in germline DNA. Variable and joining segments recombine during the development of T cells to form rearranged genes. The diversity of human gamma genes produced by this recombinational mechanism is greater than that produced by the murine genome but is more limited than that of other immunoglobulin-like genes.


Asunto(s)
Genes MHC Clase II , Linfocitos T/fisiología , Animales , ADN/genética , Humanos , Cadenas J de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/genética , Cadenas gamma de Inmunoglobulina/genética , Ratones , Hibridación de Ácido Nucleico
8.
Science ; 228(4699): 582-5, 1985 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-3983642

RESUMEN

The T-cell antigen receptor is a cell-surface molecule that participates in the immune response. In the present experiments the genes encoding the beta chain of the T-cell receptor were found to reside on the long arm of human chromosome 7 at or near band q32. Related sequences were found on the short arm of chromosome 7 in bands p15-21 in some experiments. Chromosomal rearrangements in T-cells from normal individuals and patients with ataxia telangiectasia have previously been observed at and near these map assignments for the beta-chain genes.


Asunto(s)
Mapeo Cromosómico , Receptores de Antígenos de Linfocitos T/genética , Animales , Ataxia Telangiectasia/genética , Aberraciones Cromosómicas/genética , Trastornos de los Cromosomas , Cromosomas Humanos 6-12 y X , ADN/genética , Genes , Humanos , Células Híbridas/metabolismo , Ratones , Hibridación de Ácido Nucleico
9.
Science ; 225(4659): 324-6, 1984 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-6234658

RESUMEN

The structure of rat preproatrial natriuretic factor ( preproANF ) was determined by nucleotide sequence analysis of an ANF complementary DNA clone. PreproANF is composed of a hydrophobic leader segment (20 amino acids), a precursor containing one glycosylation site (106 amino acids), and ANF (24 amino acids). Atrial natriuretic factor is located at the carboxyl terminus of the precursor molecule. The human, mouse, and rat genomes each contain a single ANF gene which is highly conserved.


Asunto(s)
Clonación Molecular , ADN/genética , Proteínas Musculares/genética , Natriuresis , Animales , Factor Natriurético Atrial , Secuencia de Bases , Hibridación de Ácido Nucleico , Ratas
10.
J Clin Invest ; 76(3): 1283-5, 1985 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-2995449

RESUMEN

Abnormal T cell function is a feature of a spectrum of inherited and acquired diseases. We have detected a frequent restriction fragment length polymorphism in the human T cell antigen receptor beta-chain locus that may aid in the analysis of these disorders. A study of a panel of 18 normal individuals, testing for the presence of the polymorphism, showed it to account for 36% of the alleles in that group. In view of the fact that the T cell receptor beta-chain locus has been mapped to chromosome 7, and that the disease ataxia telangiectasia (AT) is associated both with abnormal T cell function and with chromosomal abnormalities of the same region of chromosome 7, we investigated the possibility that the polymorphism could demonstrate linkage of the T cell receptor locus to the gene for that disease. We demonstrated that the mutation causing AT did not lie within the beta-chain locus itself, and that there was preliminary evidence that the two loci were not closely linked. This polymorphism may provide a useful tool for the study of other genetic disorders associated with abnormalities of T cell function, as well as disorders associated with inherited or acquired abnormalities of chromosome 7.


Asunto(s)
Enzimas de Restricción del ADN/metabolismo , Hibridación de Ácido Nucleico , Polimorfismo Genético , Receptores de Antígenos de Linfocitos T/genética , Ataxia Telangiectasia/genética , Mapeo Cromosómico , Genes MHC Clase II , Humanos , Regiones Constantes de Inmunoglobulina/genética , Linaje
11.
Mol Cell Biol ; 6(9): 3207-14, 1986 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-3491297

RESUMEN

The gene encoding the beta chain of the human T-cell receptor for antigen is composed of variable (V), diversity (D), joining (J), and constant (C) gene segments which undergo specific rearrangements during T-lymphocyte ontogeny. Southern blot analyses of seven human T-cell tumor lines and normal human T-lymphocyte clones revealed that most of these T-cell lines rearrange their Ti beta genes differently. The T-cell tumor line HPB-MLT rearranges and transcribes both of its Ti beta genes. Cloning and sequencing of the Ti beta cDNAs corresponding to these rearrangements revealed that one of the rearranged Ti beta genes is defective, while the other is functional and corresponds to the Ti beta protein expressed on the surface of these cells. Thus, this cell line displays a pattern of allelic exclusion of Ti beta gene expression. A comparison of four C beta 2-containing Ti beta cDNAs from three different cell lines revealed that three of the four utilize the same J beta 2.5 gene segment joined to different D beta and V beta genes, suggesting that there may be preferential use of this J gene during J beta 2 rearrangements. Hybridization analyses with probes for the alpha and beta genes of the T-cell receptor and the T-cell-specific T gamma gene revealed that HPB-MLT cells appear to express approximately equivalent amounts of RNA corresponding to each of the rearranged Ti alpha and Ti beta genes. However, they express a much lower level of T gamma RNA.


Asunto(s)
Alelos , Genes , Receptores de Antígenos de Linfocitos T/genética , Linfocitos T/inmunología , Secuencia de Aminoácidos , Secuencia de Bases , Línea Celular , Células Clonales , Clonación Molecular , ADN/análisis , Humanos , Regiones Constantes de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/genética , Leucemia/inmunología
12.
Hypertension ; 7(3 Pt 2): I31-4, 1985.
Artículo en Inglés | MEDLINE | ID: mdl-3158606

RESUMEN

The molecular biology of human atrial natriuretic factor was studied. A cloned rat cDNA probe was used to analyze tissue for the synthesis of atrial natriuretic factor, and the human gene was identified and sequenced. Nucleotide sequence comparison of human and rodent atrial natriuretic factor genes suggests regions that are critical for regulated expression of this cardiac hormone.


Asunto(s)
Proteínas Musculares/genética , Secuencia de Aminoácidos , Animales , Factor Natriurético Atrial , Clonación Molecular , ADN/genética , Humanos , Ratones , Conformación Molecular , Proteínas Musculares/biosíntesis , Nucleótidos/análisis , ARN Mensajero/genética
13.
Int J Radiat Oncol Biol Phys ; 19(2): 249-57, 1990 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-2118492

RESUMEN

Lymphoma of the conjunctiva is rare. It presents in older patients as a mass lesion and usually remains localized. Surgery is limited to biopsy, and radiation therapy is the definitive treatment of choice. The entire conjunctiva is treated. Relatively high doses (approximately 30 Gy) are required for local control, which may lead to cataract formation. Twelve patients with conjunctival lymphoma were treated at the Massachusetts General Hospital between 1979 and 1988. Ten of 12 patients presented with a unilateral lesion; 2 of 12 with bilateral lesions. Two of 12 patients were found to have systemic disease at the time of presentation. One patient developed conjunctival lymphoma 5 years after the diagnosis of generalized disease. Using electron beam, all patients were treated with a single anterior circular field to total doses ranging from 24 Gy to 30 Gy delivered in 8 to 16 fractions over 9 to 20 days. In all cases, the lens was shielded by a specially designed plastic contact lens bearing a 12 mm diameter lead shield. The lens dose was determined at varying depths beneath the shield for 6 MeV and 9 MeV electron beams and ranged from a minimum of 5% to an absolute maximum of 18% of the total dose delivered to the tumor. Local control was maintained in all patients with follow-up to 9 1/2 years. One patient relapsed distantly 3 years after treatment. One of 12 patients died of systemic disease 4 years after treatment of the ocular lesion. Two patients developed cataracts 4 and 5 years after treatment; one had bilateral cataract, although only one eye had been treated. Both patients were over 75 years old. In both cases, the cataracts were felt to be senile cataracts which are ophthalmologically and radiographically distinguishable from radiation induced lesions.


Asunto(s)
Neoplasias de la Conjuntiva/radioterapia , Leucemia Linfocítica Crónica de Células B/radioterapia , Adulto , Anciano , Anciano de 80 o más Años , Electrones , Femenino , Humanos , Masculino , Persona de Mediana Edad , Radioterapia de Alta Energía
14.
J Neuroimmunol ; 22(1): 63-8, 1989 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-2465314

RESUMEN

The frequency of virus-specific T cells in the cerebrospinal fluid of a patient with viral infection of the brain and meninges was determined by using a single-T-cell cloning technique where a representative sampling of T cells was cloned from the cerebrospinal fluid of a patient with varicella zoster viral (VZV) meningoencephalitis. That the derived T-cell clones were in fact clonal was shown by demonstrating, on Southern blot analyses, unique rearrangements of the T-cell antigen-receptor beta-chain genes of each clone. Five out of the 15 of the T4+ (CD4), 0/4 of the T8+ (CD8), and 0/1 of the T4+T8+ T-cell clones proliferated to VZV, while no clones proliferated to mumps virus or myelin basic protein. There was no clonal expansion of any VZV-reactive T cell in this patient's cerebrospinal fluid. As VZV meningoencephalitis is thought to be due to the reactivation of a dormant herpes zoster viral infection, it can be regarded as a secondary immune response. The presence of different T-cell receptor beta-chain gene rearrangements in each T-cell clone suggests that the T-cell response was polyclonal. These results demonstrate that a high frequency of polyclonal, T4+ antigen-specific T cells can be found in a naturally occurring, localized, immune response.


Asunto(s)
Herpes Zóster , Herpesvirus Humano 3/inmunología , Meningoencefalitis/líquido cefalorraquídeo , Linfocitos T/análisis , Antígenos de Diferenciación de Linfocitos T/análisis , ADN , Epítopos , Antígenos de Histocompatibilidad Clase II/análisis , Humanos , Masculino , Meningoencefalitis/etiología , Meningoencefalitis/inmunología , Meningoencefalitis/microbiología , Persona de Mediana Edad , Hibridación de Ácido Nucleico , Fenotipo , Linfocitos T/inmunología
16.
Proc Natl Acad Sci U S A ; 83(13): 4890-4, 1986 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-3487785

RESUMEN

Two unusual rearrangements of the T-cell antigen receptor beta-chain gene have occurred in the human T-cell tumor line CEM. The beta chain of the T-cell antigen receptor is encoded in germ-line DNA by immunoglobulin-like gene segments that rearrange during the somatic development of T cells to form active genes. Structural analysis of rearranged immunoglobulin genes has already revealed a great deal about the mechanisms by which these genes rearrange. To further characterize the mechanism by which beta-chain genes rearrange, we have determined the organization of the rearranged beta-chain gene segments in the human T-cell tumor line CEM. Three rearranged joining (J) or diversity (D) segments of the beta-chain gene are found in CEM. One of these segments rearranged during the formation of a normal rearranged beta-chain gene that comprises a variable (V beta), D beta, and J beta gene segment associated with a constant region gene segment. Two abnormal recombination products are found at the other rearranged beta-chain locus. One product has the structure, J beta-D beta-J beta, with the J beta gene segments joined in a head-to-head fashion, while the other one consists of a V beta-D beta recombined segment not associated with a J beta gene segment. We propose that the J beta-D beta-J beta structure was formed by an inversion of 6 kilobases of DNA and subsequently, a V beta-D beta rearrangement occurred. The presence of these products in CEM has important implications for our understanding of the mechanism by which somatic rearrangements of beta-chain gene segments occur.


Asunto(s)
Receptores de Antígenos de Linfocitos T/inmunología , Linfocitos T/fisiología , Secuencia de Bases , Diferenciación Celular , Línea Celular , Mapeo Cromosómico , Clonación Molecular , Genes , Humanos , Hibridación de Ácido Nucleico , Recombinación Genética , Linfocitos T/citología
17.
Curr Protoc Mol Biol ; Chapter 6: Unit6.4, 2001 May.
Artículo en Inglés | MEDLINE | ID: mdl-18265259

RESUMEN

The protocols in this unit describe procedures for using mixtures of 32P-labeled oligonucleotides to screen recombinant DNA clones bound to nitrocellulose filters. A partial amino acid sequence of a protein is used to predict the nucleotide sequence of the gene that would encode it. A mixture of oligonucleotides is chosen that includes all possible nucleotide sequences encoding that amino acid sequence. This mixture of oligonucleotides is then used to screen a recombinant DNA library for the corresponding clones. In some cases however, the exact nucleotide sequence of a desired clone is known and it is possible to use a unique oligonucleotide as a probe.


Asunto(s)
Sondas de Oligonucleótidos , Oligonucleótidos/síntesis química , Autorradiografía , Citratos , Indicadores y Reactivos , Marcaje Isotópico/métodos , Hibridación de Ácido Nucleico , Oligonucleótidos/química , Radioisótopos de Fósforo , Compuestos de Amonio Cuaternario , Cloruro de Sodio , Citrato de Sodio , Termodinámica
18.
J Clin Microbiol ; 27(3): 417-21, 1989 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-2654177

RESUMEN

T lymphocytes from a patient with Shigella flexneri dysentery and postdysenteric reactive arthritis were cloned by limiting dilution with recombinant interleukin-2 and a strain of S. flexneri different from that which had infected her. Five of eight clones produced proliferated in response to the shigellae used to generate the clones. The response required irradiated syngeneic blood mononuclear cells as antigen-presenting cells. One such clone, MC12, proliferated in response to both the shigellae used to generate the clones and the infecting shigellae but not to other shigellae, Salmonella heidelberg, or control Escherichia coli. MC12 was CD3+, CD4+, CD8-, and human histocompatibility leukocyte antigen (HLA)-DR+. The proliferative response to the shigellae was blocked by antibody to HLA-DR but not by antibody to HLA-A,B,C. The response required antigen-presenting cells that shared HLA-DR antigens with the clone and appeared to be restricted by HLA-DR2. The epitope recognized by MC12 was associated with the bacterial membranes. Thus, T-lymphocyte clones that proliferate in response to some shigellae can be isolated from patients with shigellosis.


Asunto(s)
Artritis Infecciosa/inmunología , Disentería Bacilar/inmunología , Shigella flexneri/inmunología , Linfocitos T/inmunología , Artritis Infecciosa/etiología , Células Clonales , Disentería Bacilar/complicaciones , Femenino , Citometría de Flujo , Humanos , Activación de Linfocitos , Persona de Mediana Edad
19.
J Immunol ; 141(11): 3726-36, 1988 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-2846692

RESUMEN

The capacity of human peripheral blood-derived T cell clones to carry out a variety of functions was examined. T cell clones were generated by stimulating individual peripheral blood T cells with PHA by a procedure that yielded a growing clone from a mean of greater than 92% of the cultured cells. A total of 65 T cell clones (44 CD4+ and 21 CD8+) generated from two individual donors were examined for their functional capabilities. All T cell clones examined secreted IL-2, IFN-gamma, and lymphotoxin/tumor necrosis factor like activity when stimulated with immobilized mAb to the CD3 complex (64.1). When 54 additional T cell clones from a third donor were analyzed, all were found to produce IL-2. Upon activation with immobilized 64.1, all CD4+ clones and 91% of the CD8+ clones induced the generation of Ig-secreting cells from purified B cells. The CD8+ clones that did not serve as Th cells alone were able to augment the capacity of fresh CD4+ cells to generate Ig-secreting cells. Each of these clones was also found to effect MHC-unrestricted cytotoxicity upon activation with immobilized 64.1. The CD8+ clones were somewhat more effective killers than CD4+ clones, although there was considerable overlap. A total of 18 clones was analyzed for TCR beta-chain gene rearrangement. Of the clones exhibiting rearrangements of the beta-chain gene, 94% were found to have a single rearrangement pattern. Finally, the detailed phenotype of 15 (11 CD4+ and 4 CD8+) of these clones was examined. Variable numbers of cells of each of the clones expressed Ag identified by mAb 4B4 (CD29), Leu 8, Leu 15 (CD11b), and NKH1. Moreover, cells of 6 of 11 CD4+ clones and 4 of 4 CD8+ clones also expressed CD45R in addition to CD29; expression of CD45R and CD29 varied with the activation status of the clone. The current data demonstrate that nearly all of the T cell clones were able to accomplish each of the functions examined regardless of the surface phenotype. Inasmuch as the clones were generated using a technique that expanded more than 92% of the circulating T cells, the data imply that the progeny of the vast majority of T cells may have the inherent capacity to exert a wide array of functional activities.


Asunto(s)
Células Clonales/clasificación , Fenotipo , Linfocitos T/clasificación , Adulto , Antígenos de Diferenciación/análisis , Diferenciación Celular , Células Clonales/inmunología , Células Clonales/metabolismo , Pruebas Inmunológicas de Citotoxicidad , Reordenamiento Génico de la Cadena beta de los Receptores de Antígenos de los Linfocitos T , Antígenos HLA/genética , Antígenos de Histocompatibilidad/análisis , Humanos , Interferón gamma/biosíntesis , Interleucina-2/biosíntesis , Antígenos Comunes de Leucocito , Activación de Linfocitos , Linfotoxina-alfa/biosíntesis , Linfocitos T/inmunología , Linfocitos T/metabolismo , Linfocitos T Colaboradores-Inductores/clasificación , Linfocitos T Colaboradores-Inductores/inmunología , Factor de Necrosis Tumoral alfa/biosíntesis
20.
Proc Natl Acad Sci U S A ; 83(19): 7434-7, 1986 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-3094008

RESUMEN

Characterization of tumors that arise spontaneously in the AKR mouse indicates that they are derived from cells of a distinct T-cell lineage. Cells in this subclass bear surface antigens, designated Tpre, Tthy, Tind, and Tsu, which are encoded by genes in the Tsu linkage group on murine chromosome 12. We have examined the rearrangement and expression of genes encoding the T-cell alpha, beta, and gamma chains in these tumors. Although these cells contain alpha-chain mRNA, they do not produce a normal-sized beta-chain mRNA. Most of them also lack gamma-chain mRNA. Each thymic leukemia was derived from a cell arrested at a different stage of development as defined by their expression of terminal deoxynucleotidyl transferase and Thy-1 mRNA. The data presented here are consistent with a model in which thymocytes expressing Tpre, Tthy, Tind, or Tsu undergo somatic development parallel to the development of other T cells. However, these thymocytes do not appear to differentiate into cells bearing alpha-beta heterodimers of the T-cell antigen receptor.


Asunto(s)
Leucemia Experimental/patología , Receptores de Antígenos de Linfocitos T/genética , Linfocitos T/inmunología , Animales , Antígenos de Diferenciación de Linfocitos T , Antígenos de Superficie/genética , Diferenciación Celular , ADN Nucleotidilexotransferasa/genética , Regulación de la Expresión Génica , Genes , Leucemia Experimental/genética , Leucemia Experimental/inmunología , Sustancias Macromoleculares , Ratones , Ratones Endogámicos AKR , ARN Mensajero/genética , Recombinación Genética , Linfocitos T/citología , Linfocitos T/fisiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA