RESUMEN
As an adjunct to the genomic sequencing of Caenorhabditis elegans, we have investigated a representative cDNA library of 1,517 clones. A single sequence read has been obtained from the 5' end of each clone, allowing its characterization with respect to the public databases, and the clones are being localized on the genome map. The result is the identification of about 1,200 of the estimated 15,000 genes of C. elegans. More than 30% of the inferred protein sequences have significant similarity to existing sequences in the databases, providing a route towards in vivo analysis of known genes in the nematode. These clones also provide material for assessing the accuracy of predicted exons and splicing patterns and will lead to a more accurate estimate of the total number of genes in the organism than has hitherto been available.
Asunto(s)
Caenorhabditis elegans/genética , Genes de Helminto , Animales , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , ADN/genética , Sondas de ADN , Expresión Génica , Biblioteca de Genes , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Lugares Marcados de SecuenciaRESUMEN
The Huntington's disease (HD) gene encodes a novel protein with as yet no known function. In order to identify the functionally important domains of this protein, we have cloned and sequenced the homologue of the HD gene in the pufferfish, Fugu rubripes. The Fugu HD gene spans only 23 kb of genomic DNA, compared to the 170 kb human gene, and yet all 67 exons are conserved. The first coding exon, the site of the disease-causing triplet repeat, is highly conserved. However, the glutamine repeat in Fugu consists of just four residues. We also show that gene order may be conserved over longer stretches of the two genomes. Our work describes a detailed example of sequence comparison between human and Fugu, and illustrates the power of the pufferfish genome as a model system in the analysis of human genes.
Asunto(s)
Peces Venenosos/genética , Enfermedad de Huntington/genética , Proteínas del Tejido Nervioso/genética , Proteínas Nucleares/genética , Homología de Secuencia , Secuencia de Aminoácidos , Animales , Clonación Molecular , Codón/genética , Secuencia Conservada , ADN Complementario , Exones , Humanos , Proteína Huntingtina , Ratones , Datos de Secuencia Molecular , Secuencias Repetitivas de Ácidos Nucleicos , Alineación de SecuenciaRESUMEN
A set of programs has been developed for rapid collection of x-ray intensity data from protein and virus crystals with a commercially available two-dimensional focused geometry electronic detector. The detector is compact and portable, with unusually high spatial resolution comparable to that used in oscillation photography. It has allowed x-ray data collection on weakly diffracting crystals with large unit cells, as well as more conventional "diffractometer-quality" crystals. The quality of the data is compared with that from oscillation photography and automated diffractometry in the range of unit cells from 96.3 to 383.2 angstroms. Isomorphous and anomalous difference Pattersons, based on detector data, are shown for a variable surface glycoprotein mercury derivative and for a repressor-DNA bromine derivative, which has been solved at 7 angstroms with detector data only.
Asunto(s)
ADN , Proteínas , Virus/ultraestructura , Difracción de Rayos X/métodos , Computadores , Matemática , Conformación de Ácido Nucleico , Conformación Proteica , Difracción de Rayos X/instrumentaciónRESUMEN
The Ensembl (http://www.ensembl.org/) project provides a comprehensive and integrated source of annotation of chordate genome sequences. Over the past year the number of genomes available from Ensembl has increased from 15 to 33, with the addition of sites for the mammalian genomes of elephant, rabbit, armadillo, tenrec, platypus, pig, cat, bush baby, common shrew, microbat and european hedgehog; the fish genomes of stickleback and medaka and the second example of the genomes of the sea squirt (Ciona savignyi) and the mosquito (Aedes aegypti). Some of the major features added during the year include the first complete gene sets for genomes with low-sequence coverage, the introduction of new strain variation data and the introduction of new orthology/paralog annotations based on gene trees.
Asunto(s)
Bases de Datos de Ácidos Nucleicos , Genómica , Animales , Secuencia de Bases , Bases de Datos de Ácidos Nucleicos/normas , Variación Genética , Genoma Humano , Humanos , Internet , Ratones , Proteínas/genética , Estándares de Referencia , Alineación de Secuencia , Integración de Sistemas , Interfaz Usuario-ComputadorRESUMEN
The Ensembl (http://www.ensembl.org/) project provides a comprehensive and integrated source of annotation of large genome sequences. Over the last year the number of genomes available from the Ensembl site has increased from 4 to 19, with the addition of the mammalian genomes of Rhesus macaque and Opossum, the chordate genome of Ciona intestinalis and the import and integration of the yeast genome. The year has also seen extensive improvements to both data analysis and presentation, with the introduction of a redesigned website, the addition of RNA gene and regulatory annotation and substantial improvements to the integration of human genome variation data.
Asunto(s)
Bases de Datos de Ácidos Nucleicos , Genómica , Animales , Secuencia de Bases , Variación Genética , Genoma Humano , Humanos , Internet , Ratones , Proteínas/genética , ARN/genética , Ratas , Secuencias Reguladoras de Ácidos Nucleicos , Alineación de Secuencia , Interfaz Usuario-ComputadorRESUMEN
KB cells express a folate-binding protein that is anchored to the plasma membrane by a glycosylated phosphatidylinositol (GPI) tail and these cells can grow in medium containing a very low folate concentration (1 nM). In contrast, mouse 3T3 cells do not express a membrane-associated folate-binding protein and cannot grow under similar low folate conditions. In these studies, 3T3 cells were transfected with a vector containing the cDNA that codes for the KB cell folate-binding protein. In contrast to the wild-type 3T3 cells, the transfected 3T3 cells express a level of folate-binding protein similar to KB cells, 1 and 1.4 ng/micrograms protein, respectively. The capacity for binding [3H] folate to the surface of transfected 3T3 cells cultured in folate-deficient medium is 7.7 pmol/10(6) cells, and this is approximately 50% of the surface binding capacity of KG cells under similar culture conditions. Moreover, after treatment of the transfected 3T3 cells with phospholipase C specific for phosphatidylinositol, the binding of [3H] folate to the surface of these cells is reduced by 90%, indicating that, like the KB cells, the folate-binding protein is anchored to the plasma membrane by a GPI tail. Although the doubling time of wild-type 3T3 cells markedly increases after 13 d of culture in folate-deficient medium, the doubling time of both the transfected 3T3 cells and KB cells do not change. The results of these experiments indicate that the GPI-anchored folate-binding protein provides a mechanism to maintain a level of folate that permits the folate-dependent metabolic functions necessary for cell survival under low folate conditions.
Asunto(s)
Proteínas Portadoras/fisiología , ADN/genética , Ácido Fólico/farmacología , Glucolípidos/fisiología , Fosfatidilinositoles/fisiología , Receptores de Superficie Celular , Transfección , Células 3T3 , Animales , Secuencia de Bases , Southern Blotting , Proteínas Portadoras/biosíntesis , Proteínas Portadoras/genética , Supervivencia Celular , Receptores de Folato Anclados a GPI , Ácido Fólico/metabolismo , Glicosilfosfatidilinositoles , Humanos , Células KB , Ratones , Datos de Secuencia MolecularRESUMEN
We found that the 5' nontranslated leader sequence from encephalomyocarditis virus (EMCV) allowed transcripts that were synthesized by the T3 RNA polymerase in mammalian cells to be translated in a cap-independent fashion. Stable mouse cell lines that carry the T3 RNA polymerase gene expressed the chloramphenicol acetyltransferase (CAT) gene under the control of a phage promoter when the CAT gene was fused to the EMCV leader and introduced into the cells by transient DNA uptake. The level of gene expression in such cells was similar to or greater than that observed with a conventional transient expression vector that is dependent on transcription by the host RNA polymerase II. Expression of the EMCV-CAT fusion gene was stimulated by cotransfection of the cells with a gene that encodes the poliovirus protease 2A protein (which inhibits cap-dependent translation), demonstrating that the EMCV-CAT fusion gene was expressed in a cap-independent fashion. Introduction of both the T3 RNA polymerase gene and the EMCV-CAT fusion gene into a variety of cultured mammalian cell lines (HeLa, BSC40, Ltk-, NIH 3T3, and C127) demonstrated that the T3-EMCV expression system functions in a broad range of cell types.
Asunto(s)
ARN Polimerasas Dirigidas por ADN/genética , Escherichia coli/genética , ARN Mensajero/biosíntesis , Fagos T/genética , Animales , Secuencia de Bases , Línea Celular , Cloranfenicol O-Acetiltransferasa/genética , Cloranfenicol O-Acetiltransferasa/metabolismo , ARN Polimerasas Dirigidas por ADN/metabolismo , Virus de la Encefalomiocarditis/genética , Genes Virales , Datos de Secuencia Molecular , Sondas de Oligonucleótidos , Plásmidos , Regiones Promotoras Genéticas , ARN Mensajero/metabolismo , Fagos T/enzimología , Transfección , Virus Vaccinia/genética , Proteínas Estructurales Virales/genéticaRESUMEN
The Ensembl (http://www.ensembl.org/) project provides a comprehensive and integrated source of annotation of large genome sequences. Over the last year the number of genomes available from the Ensembl site has increased by 7 to 16, with the addition of the six vertebrate genomes of chimpanzee, dog, cow, chicken, tetraodon and frog and the insect genome of honeybee. The majority have been annotated automatically using the Ensembl gene build system, showing its flexibility to reliably annotate a wide variety of genomes. With the increased number of vertebrate genomes, the comparative analysis provided to users has been greatly improved, with new website interfaces allowing annotation of different genomes to be directly compared. The Ensembl software system is being increasingly widely reused in different projects showing the benefits of a completely open approach to software development and distribution.
Asunto(s)
Bases de Datos de Ácidos Nucleicos , Genómica , Animales , Secuencia de Bases , Bovinos , Perros , Humanos , Internet , Ratones , Ratas , Alineación de Secuencia , Programas Informáticos , Interfaz Usuario-ComputadorRESUMEN
The nematode worm C. elegans, with its transparent body, is an excellent vehicle for studying developmental gene expression during embryogenesis and throughout its short life. Expression data from in-situ hybridization, immunolocalization and reporter constructs have been put into the ACeDB database, which is used to store and disseminate most types of C. elegans data, and is also widely used for genome-sequencing projects. In the database, the gene-expression patterns are linked to genes, sequences, cells, organs and the developmental stage in which expression occurs. An accessory program 'Angler' can be used to browse sectional Nomarski images of the worm embryo during early development, and to relate these images to overlaid cell lineage data and 3-D schematic views of cell positions.Copyright 1997 Academic Press Limited Copyright 1997Academic Press Limited
RESUMEN
U12-dependent introns are found in small numbers in most eukaryotic genomes, but their scarcity makes accurate characterisation of their properties challenging. A computational search for U12-dependent introns was performed using the draft version of the human genome sequence. Human expressed sequences confirmed 404 U12-dependent introns within the human genome, a 6-fold increase over the total number of non-redundant U12-dependent introns previously identified in all genomes. Although most of these introns had AT-AC or GT-AG terminal dinucleotides, small numbers of introns with a surprising diversity of termini were found, suggesting that many of the non-canonical introns found in the human genome may be variants of U12-dependent introns and, thus, spliced by the minor spliceosome. Comparisons with U2-dependent introns revealed that the U12-dependent intron set lacks the 'short intron' peak characteristic of U2-dependent introns. Analysis of this U12-dependent intron set confirmed reports of a biased distribution of U12-dependent introns in the genome and allowed the identification of several alternative splicing events as well as a surprising number of apparent splicing errors. This new larger reference set of U12-dependent introns will serve as a resource for future studies of both the properties and evolution of the U12 spliceosome.
Asunto(s)
Biología Computacional/métodos , Genoma Humano , Intrones , Ribonucleoproteínas Nucleares Pequeñas/fisiología , Empalme Alternativo , Humanos , Sitios de Empalme de ARN , Ribonucleoproteína Nuclear Pequeña U2/fisiologíaRESUMEN
WormBase (http://www.wormbase.org) is a web-based resource for the Caenorhabditis elegans genome and its biology. It builds upon the existing ACeDB database of the C.elegans genome by providing data curation services, a significantly expanded range of subject areas and a user-friendly front end.
Asunto(s)
Caenorhabditis elegans/genética , Bases de Datos Factuales , Empalme Alternativo , Animales , Linaje de la Célula , Regulación de la Expresión Génica , Genes de Helminto/genética , Genoma , Servicios de Información , InternetRESUMEN
Signature databases are vital tools for identifying distant relationships in novel sequences and hence for inferring protein function. InterPro is an integrated documentation resource for protein families, domains and functional sites, which amalgamates the efforts of the PROSITE, PRINTS, Pfam and ProDom database projects. Each InterPro entry includes a functional description, annotation, literature references and links back to the relevant member database(s). Release 2.0 of InterPro (October 2000) contains over 3000 entries, representing families, domains, repeats and sites of post-translational modification encoded by a total of 6804 different regular expressions, profiles, fingerprints and Hidden Markov Models. Each InterPro entry lists all the matches against SWISS-PROT and TrEMBL (more than 1,000,000 hits from 462,500 proteins in SWISS-PROT and TrEMBL). The database is accessible for text- and sequence-based searches at http://www.ebi.ac.uk/interpro/. Questions can be emailed to interhelp@ebi.ac.uk.
Asunto(s)
Bases de Datos Factuales , Proteínas , Servicios de Información , Internet , Estructura Terciaria de Proteína , Proteínas/química , Proteínas/genéticaRESUMEN
The Ensembl (http://www.ensembl.org/) database project provides a bioinformatics framework to organise biology around the sequences of large genomes. It is a comprehensive source of stable automatic annotation of the human genome sequence, with confirmed gene predictions that have been integrated with external data sources, and is available as either an interactive web site or as flat files. It is also an open source software engineering project to develop a portable system able to handle very large genomes and associated requirements from sequence analysis to data storage and visualisation. The Ensembl site is one of the leading sources of human genome sequence annotation and provided much of the analysis for publication by the international human genome project of the draft genome. The Ensembl system is being installed around the world in both companies and academic sites on machines ranging from supercomputers to laptops.
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Bases de Datos Genéticas , Genoma Humano , Biología Computacional , Sistemas de Administración de Bases de Datos , Humanos , Almacenamiento y Recuperación de la Información , Internet , Análisis de Secuencia de ADN , Integración de SistemasRESUMEN
The Ensembl (http://www.ensembl.org/) database project provides a bioinformatics framework to organise biology around the sequences of large genomes. It is a comprehensive source of stable automatic annotation of human, mouse and other genome sequences, available as either an interactive web site or as flat files. Ensembl also integrates manually annotated gene structures from external sources where available. As well as being one of the leading sources of genome annotation, Ensembl is an open source software engineering project to develop a portable system able to handle very large genomes and associated requirements. These range from sequence analysis to data storage and visualisation and installations exist around the world in both companies and at academic sites. With both human and mouse genome sequences available and more vertebrate sequences to follow, many of the recent developments in Ensembl have focusing on developing automatic comparative genome analysis and visualisation.
Asunto(s)
Bases de Datos Genéticas , Genómica , Animales , Biología Computacional , Genoma Humano , Humanos , Internet , Ratones , Programas Informáticos , SinteníaRESUMEN
The Ensembl (http://www.ensembl.org/) database project provides a bioinformatics framework to organize biology around the sequences of large genomes. It is a comprehensive and integrated source of annotation of large genome sequences, available via interactive website, web services or flat files. As well as being one of the leading sources of genome annotation, Ensembl is an open source software engineering project to develop a portable system able to handle very large genomes and associated requirements. The facilities of the system range from sequence analysis to data storage and visualization and installations exist around the world both in companies and at academic sites. With a total of nine genome sequences available from Ensembl and more genomes to follow, recent developments have focused mainly on closer integration between genomes and external data.
Asunto(s)
Biología Computacional , Bases de Datos Genéticas , Genoma , Genómica , Animales , Humanos , Almacenamiento y Recuperación de la Información , Internet , Programas InformáticosRESUMEN
The effects of tentoxin on the ATPase activities of coupling factor 1 proteins (CF1) and photophosphorylation with isolated chloroplasts and chloroplasts reconstituted with coupling factor proteins have been examined. 1. The calcium-dependent ATPase activities of coupling factors isolated from spinach, lettuce and Nicotiana otophora are completely inhibited by tentoxin. The ATPase activities of coupling factors isolated from Nicotiana tabacum and Nicotiana knightiana are not affected by tentoxin. 2. Phenazine methosulfate-catalyzed cyclic photophosphorylation with chloroplasts isolated from spinach, lettuce and N. otophora is completely inhibited by tentoxin, whereas chloroplasts isolated from N. knightiana and N. tabacum are relatively insensitive to tentoxin. 3. Spinach chloroplasts, partially depleted in CF1, can be reconstituted with coupling factors isolated from a wide variety of plants including lettuce, radish, N. tabacum, N. knightiana and N. otophora. 4. Spinach chloroplasts reconstituted with spinach, lettuce and N. otophora CF1 retain their sensitivity to tentoxin; however, when reconstituted with N. knightiana and N. tabacum coupling factor proteins, a significant fraction of the reconstituted rate remains tentoxin insensitive. These data are interpreted as evidence that coupling factors that reconstitute with spinach thylakoid membranes have both a catalytic and structural function.
Asunto(s)
Cloroplastos/metabolismo , Péptidos Cíclicos/farmacología , ATPasas de Translocación de Protón/fisiología , Adenosina Trifosfatasas/metabolismo , Alternaria , Catálisis , Cloroplastos/efectos de los fármacos , Membranas/metabolismo , Micotoxinas/farmacología , Fotofosforilación/efectos de los fármacos , Plantas , Especificidad de la EspecieRESUMEN
ATP, added to the solution bathing the nutrient (serosal) surface of isolated frog gastric mucosa, was found to break down rapidly to ADP, inorganic phosphate and other products. This activity is due to an ectoenzyme, i.e. to an enzyme system easily accessible to the bathing solution. This conclusion follows from experiments which showed that penetration of ATP into the mucosal cells occurred at a much slower rate: leakage of inorganic phosphate and adenine nucleotides from mucosal cells was also minor. The surface ATPase may reflect the operation of a mechanism at the nutrient surface involved in acid secretion.
Asunto(s)
Adenosina Trifosfato/metabolismo , Mucosa Gástrica/metabolismo , Adenosina Trifosfatasas/metabolismo , Animales , Anuros , Medios de Cultivo , Técnicas In Vitro , Cinética , Rana catesbeianaRESUMEN
Stomach and gallbladder actively transport fluids which are nearly isotonic to plasma. Consideration of the measured areas of the appropriate transporting surfaces gives a more realistic view of the osmotic gradient required to account for the observed net flow of water. Simple osmosis may be adequate if the transporting membrane has an osmotic permeability in the range observed for synthetic lecithin-cholesterol bilayer membranes.
Asunto(s)
Vesícula Biliar/fisiología , Estómago/fisiología , Equilibrio Hidroelectrolítico , Animales , Anuros , Transporte Biológico Activo , Perros , ConejosRESUMEN
The interaction of tentoxin [cyclo-(-L-leucyl-N-methyl-(Z)-dehydrophenylalanyl-glycyl-N-methyl-L-alanyl-)] with solubilized lettuce chloroplast coupling factor 1 was characterized by direct binding studies, measurement of the time course of ATPase inhibition, and steady-state enzyme kinetics. Neither substrates, products or Ca2+ competed with the tentoxin binding site, nor did they induce any large change in tentoxin affinity. The inhibition of lettuce chloroplast coupling factor 1 ATPase was found to be the time dependent, and at equilibrium the affinities estimated by equilibrium ultrafiltration and enzyme inhibition were similar (1.8 . 10(8) M-1). The steady-state kinetics best fit an uncompetitive pattern suggesting that the inhibited steps follow an irreversible step occurring after ATP binding.
Asunto(s)
Micotoxinas/farmacología , ATPasas de Translocación de Protón/metabolismo , Adenosina Difosfato/farmacología , Adenosina Trifosfatasas/metabolismo , Adenosina Trifosfato/farmacología , Sitios de Unión , Calcio/farmacología , Relación Dosis-Respuesta a Droga , Cinética , Péptidos Cíclicos/farmacología , Fotofosforilación , Plantas , TripsinaRESUMEN
Tentoxin at 10--1000 micrometer causes a marked species-selective stimulation of coupling factor 1 Ca2+-dependent ATPase activity (Ka 6.3 . 10(3) M-1). This effect decreases the Km for ATP to about 0.3 mM and increases V 2.75-fold. Above 1.6 micrometer tentoxin the rate of coupled electron transport was reduced to basal without uncoupling.