RESUMEN
Electrostatic profiles for sigma70-specific promoters of T4 bacteriophage and four consensus-like synthetic promoters have been calculated. It was shown that the promoters can be classified according to the presence of some specific electrostatic elements located in the far upstream region of their DNAs. A correlation between the electrostatic elements specific for promoters and their functional behaviour was established. A general scheme of promotor activity regulation through electrostatic interactions between the upstream region of promoter DNA and the RNA-polymerase a-subunit was suggested.
Asunto(s)
Bacteriófago T4/genética , ARN Polimerasas Dirigidas por ADN/metabolismo , Regulación Viral de la Expresión Génica , Regiones Promotoras Genéticas , ARN Polimerasas Dirigidas por ADN/química , Factor sigma/metabolismo , Electricidad EstáticaRESUMEN
A comparative analysis of electrostatic potential distribution for "early" T4 phage promoters was undertaken. The data obtained indicate that there are some particular elements in the patterns of electrostatic potential distribution of promoter DNA specific for promoter groups differing by their functional response to ADP-ribosylation of the alpha-subunit as well as to rpoB403- or rpoB409 mutationals of the beta-subunit of RNA-polymerase.