RESUMEN
Long-term in situ plasma membrane-targeted imaging is highly significant for investigating specific biological processes and functions, especially for the imaging and tracking of apoptosis processes of cells. However, currently developed membrane probes are rarely utilized to monitor the in situ damage of the plasma membrane. Herein, a transition-metal complex phosphorescent indicator, Ru-Chol, effectively paired with cholesterol, exhibits excellent properties on staining the plasma membrane, with excellent antipermeability, good photostability, large Stokes shift, and long luminescence lifetime. In addition, Ru-Chol not only has the potential to differentiate cancerous cells from normal cells but also tracks in real time the entire progression of cisplatin-induced plasma membrane damage and cell apoptosis. Therefore, Ru-Chol can serve as an efficient tool for the monitoring of morphological and physiological changes in the plasma membrane, providing assistance for drug screening and early diagnosis and treatment of diseases, such as immunodeficiency, diabetes, cirrhosis, and tumors.
Asunto(s)
Membrana Celular , Colesterol , Complejos de Coordinación , Rutenio , Humanos , Rutenio/química , Colesterol/química , Colesterol/análisis , Membrana Celular/química , Membrana Celular/metabolismo , Complejos de Coordinación/química , Complejos de Coordinación/síntesis química , Complejos de Coordinación/farmacología , Apoptosis/efectos de los fármacos , Sustancias Luminiscentes/química , Sustancias Luminiscentes/síntesis química , Cisplatino/farmacología , Antineoplásicos/farmacología , Antineoplásicos/química , Estructura MolecularRESUMEN
Herein, a new kind of lead halide perovskite (LHP, (C12H25NH3)2PbI4) with aggregation-induced emission (AIE) feature is developed as a fluorescent probe for heparin (Hep). The LHPs exhibit high emission when they aggregate in water. Interestingly, a few picomoles of dispersed gold nanoparticles (AuNPs) can quench the emission of LHPs, but the aggregated AuNPs are invalid. When protamine (Pro) is mixed with AuNPs at first, the negatively charged AuNPs aggregate through electrostatic interaction, producing the AIE recovery. Nevertheless, Hep disturbs the interaction between AuNPs and Pro due to its strong electrostatic interaction with Pro. Therefore, the dispersed AuNPs quench the fluorescence of LHPs again. A response linear range of Hep of 0.8-4.2 ng ml-1is obtained, and the detection limit is 0.29 ng ml-1. Compared with other probes for determination of Hep with AuNPs, this strategy exhibits better sensitivity due to the small quantity of AuNPs used. Finally, it is also successfully applied to detect Hep in human serum samples with satisfactory recoveries.