Youths Are Less Susceptible to Exercise-Induced Muscle Damage Than Adults: A Systematic Review With Meta-Analysis.

PURPOSE: This meta-analysis aimed to (1) provide a comparison of peak changes in indirect markers of exercise-induced muscle damage (EIMD) in youths versus adults and (2) determine if the involved limb moderated this effect. METHOD: Studies were eligible for inclusion if they (1) provided a human youth versus adult comparison; (2) provided data on muscle strength, soreness, or creatine kinase markers beyond ≥24 hours; and (3) did not provide a recovery treatment. Effect sizes (ES) were presented alongside 95% confidence intervals. RESULTS: EIMD exhibited larger effects on adults than in youths for muscle strength (ES = -2.01; P < .001), muscle soreness (ES = -1.52; P < .001), and creatine kinase (ES = -1.98; P < .001). The random effects meta-regression indicated that the effects of upper- and lower-limb exercise in youths and adults were significant for muscle soreness (coefficient estimate = 1.11; P < .001) but not for muscle strength or creatine kinase (P > .05). As such, the between-group effects for muscle soreness (ES = -2.10 vs -1.03; P < .05) were greater in the upper than lower limbs. CONCLUSION: The magnitude of EIMD in youths is substantially less than in their adult counterparts, and this effect is greater in upper than lower limbs for muscle soreness. These findings help guide practitioners who may be concerned about the potential impact of EIMD when training youth athletes.

Biological maturation, contextual factors, and future career in basketball: A possible interrelationship?

This study aimed to analyse the relationship between the maturational state and contextual factors with future basketball career success in Under-16 (U16) Portuguese youth preparing for the U16 European Basketball Championships. Eighty-five Under-15 (U15) and U16 male basketball players (height: 189.7 ± 6.53 cm; body mass: 81.1 ± 9.96 kg; the age of peak height velocity: 13.3 ± 0.7 y) were selected by the Portuguese national coaching staff to participate in a national team training camp in 2016, 2017, and 2018 years. A binary logistic regression was employed to identify the influence of maturation, born quartile, demographic, geographic and economic characteristics, and sport career (i.e., drop out during first season and chance of playing professionally). Early maturing players were typically classified as inside players. Training experience and predicted adult height, and type of first club were able to predict players who dropped out during their first season at senior level (both p < 0.01). No variables were able to predict who played in a professional league during their first season at senior level. Practitioners should use these data to consider how national level basketball players are identified and developed, but also keep in mind of their specific contextual factors.

Kinetic and Kinematic Assessment of the Band-Assisted Countermovement Jump.

ABSTRACT: Fernandes, JFT, Arede, J, Clarke, H, Garcia-Ramos, A, Perez-Castilla, A, Norris, JP, Wilkins, CA, and Dingley, AF. Kinetic and kinematic assessment of the band-assisted countermovement jump. J Strength Cond Res 37(8): 1588-1593, 2023-This study sought to elucidate kinetic and kinematic differences between unloaded and band-assisted countermovement jumps (CMJs). In a randomized order, 20 healthy subjects (mass 84.5 ± 18.6 kg) completed 3 repetitions of CMJs across 3 conditions: unloaded (at body mass), low, and moderate band (8.4 ± 1.9 and 13.3 ± 3.3 kg body mass reduction, respectively). For all repetitions, a force platform and linear position transducer were used to record and calculate kinetic and kinematic data. Body mass was significantly different between the unloaded, low, and moderate band conditions ( p < 0.05). Peak velocity, absolute peak, and mean force and movement duration displayed a trend that was mostly related to the condition (i.e., unloaded > low > moderate) ( p < 0.05). The opposing trend (i.e., moderate > low > unloaded) was generally observed for relative peak and mean force, reactive strength index modified, and flight time ( p < 0.05). No differences were observed for mean velocity, movement duration, and absolute and relative landing forces ( p > 0.05). The use of band assistance during CMJs can alter force, time, and velocity variables. Practitioners should be aware of the potential positive and negative effects of band assistance during CMJs.

Aging and Recovery After Resistance-Exercise-Induced Muscle Damage: Current Evidence and Implications for Future Research.

Aging is anecdotally associated with a prolonged recovery from resistance training, though current literature remains equivocal. This brief review considers the effects of resistance training on indirect markers of muscle damage and recovery (i.e., muscle soreness, blood markers, and muscle strength) in older males. With no date restrictions, four databases were searched for articles relating to aging, muscle damage, and recovery. Data from 11 studies were extracted for review. Of these, four reported worse symptoms in older compared with younger populations, while two have observed the opposite, and the remaining studies (n = 6) proposed no differences between age groups. It appears that resistance training can be practiced in older populations without concern for impaired recovery. To improve current knowledge, researchers are urged to utilize more ecologically valid muscle-damaging bouts and investigate the mechanisms which underpin the recovery of muscle soreness and strength after exercise in older populations.

Validity of Different Velocity-Based Methods and Repetitions-to-Failure Equations for Predicting the 1 Repetition Maximum During 2 Upper-Body Pulling Exercises.

ABSTRACT: Pérez-Castilla, A, Suzovic, D, Domanovic, A, Fernandes, JFT, and García-Ramos, A. Validity of different velocity-based methods and repetitions-to-failure equations for predicting the 1 repetition maximum during 2 upper-body pulling exercises. J Strength Cond Res 35(7): 1800-1808, 2021-This study aimed to compare the accuracy of different velocity-based methods and repetitions-to-failure equations for predicting the 1 repetition maximum (i.e., maximum load that can be lifted once; 1RM) during 2 upper-body pulling exercises. Twenty-three healthy subjects (twelve men and eleven women) were tested in 2 sessions during the lat pull-down and seated cable row exercises. Each session consisted of an incremental loading test until reaching the 1RM followed by a set of repetitions-to-failure against the 80% 1RM load. The 1RM was estimated from the individual load-velocity relationships modeled through 4 (∼40, 55, 70, and 85% 1RM; multiple-point method) or 2 loads (∼40 and 85% 1RM; 2-point method). Mean velocity was recorded with a linear position transducer and a Smartphone application. Therefore, 4 velocity-based methods were used as a result of combining the 2 devices and the 2 methods. Two repetitions-to-failure equations (Mayhew and Wathen) were also used to predict the 1RM from the load and number of repetitions completed. The absolute differences with respect to the actual 1RM were higher for the repetitions-to-failure equations than velocity-based methods during the seated cable row exercise (p = 0.004), but not for the lat pull-down exercise (p = 0.200). The repetitions-to-failure equations significantly underestimated the actual 1RM (p < 0.05; range: -6.65 to -2.14 kg), whereas no systematic differences were observed for the velocity-based methods (range: -1.75 to 1.65 kg). All predicted 1RMs were highly correlated with the actual 1RM (r ≥ 0.96). The velocity-based methods provide a more accurate estimate of the 1RM than the Mayhew and Wathen repetitions-to-failure equations during the lat pull-down and seated cable row exercises.

Comparison of the FitroDyne and GymAware Rotary Encoders for Quantifying Peak and Mean Velocity During Traditional Multijointed Exercises.

ABSTRACT: Fernandes, JFT, Lamb, KL, Clark, CCT, Moran, J, Drury, B, Garcia-Ramos, A, and Twist, C. Comparison of the FitroDyne and GymAware rotary encoders for quantifying peak and mean velocity during traditional multijointed exercises. J Strength Cond Res 35(6): 1760-1765, 2021-The FitroDyne and GymAware rotary encoders are being increasingly used in resistance training to monitor movement velocity, but how closely their velocity outcomes agree is unknown. Consequently, this study aimed to determine the level of agreement between the FitroDyne and GymAware for the assessment of movement velocity in 3 resistance training exercises. Fifteen men performed 3 repetitions of bench press, back squat, and bent-over-row exercises at 10% 1 repetition maximum increments (from 20 to 80%). For each repetition, the FitroDyne and GymAware recorded peak and mean barbell velocity (cm·s-1). Although strongly correlated (r = 0.79-1.00), peak velocity values for the GymAware were significantly lower than the FitroDyne for all exercises and loads. Importantly, the random errors between the devices, quantified through Bland and Altman's 95% limits of agreement, were unacceptable, ranging from ±3.8 to 25.9 cm·s-1. Differences in mean velocity were smaller (and nonsignificant for most comparisons) and highly correlated (r = 0.86-1.00) between devices. Notwithstanding smaller random errors than for the peak values, mean values still reflected poor agreement (random errors between ±2.1 and 12.0 cm·s-1). These findings suggest that the FitroDyne and GymAware cannot record peak or mean velocity with acceptable agreement and should neither be used interchangeably nor their data compared.

A Comparison of Load-Velocity and Load-Power Relationships Between Well-Trained Young and Middle-Aged Males During Three Popular Resistance Exercises.

Fernandes, JFT, Lamb, KL, and Twist, C. A comparison of load-velocity and load-power relationships between well-trained young and middle-aged males during 3 popular resistance exercises. J Strength Cond Res 32(5): 1440-1447, 2018-This study examined the load-velocity and load-power relationships among 20 young (age 21.0 ± 1.6 years) and 20 middle-aged (age 42.6 ± 6.7 years) resistance-trained males. Participants performed 3 repetitions of bench press, squat, and bent-over-row across a range of loads corresponding to 20-80% of 1 repetition maximum (1RM). Analysis revealed effects (p < 0.05) of group and load × group on barbell velocity for all 3 exercises, and interaction effects on power for squat and bent-over-row (p < 0.05). For bench press and bent-over-row, the young group produced higher barbell velocities, with the magnitude of the differences decreasing as load increased (ES; effect size 0.0-1.7 and 1.0-2.0, respectively). Squat velocity was higher in the young group than the middle-aged group (ES 1.0-1.7) across all loads, as was power for each exercise (ES 1.0-2.3). For all 3 exercises, both velocity and 1RM were correlated with optimal power in the middle-aged group (r = 0.613-0.825, p < 0.05), but only 1RM was correlated with optimal power (r = 0.708-0.867, p < 0.05) in the young group. These findings indicate that despite their resistance training, middle-aged males were unable to achieve velocities at low external loads and power outputs as high as the young males across a range of external resistances. Moreover, the strong correlations between 1RM and velocity with optimal power suggest that middle-aged males would benefit from training methods which maximize these adaptations.

Effects of Variable Resistance Using Chains on Bench Throw Performance in Trained Rugby Players.

Godwin, MS, Fernandes, JFT, and Twist, C. Effects of variable resistance using chains on bench throw performance in trained rugby players. J Strength Cond Res 32(4): 950-954, 2018-This study sought to determine the effects of variable resistance using chain resistance on bench throw performance. Eight male rugby union players (19.4 ± 2.3 years, 88.8 ± 6.0 kg, 1RM 105.6 ± 17.0 kg) were recruited from a national league team. In a randomized crossover design, participant's performed 3 bench throws at 45% one repetition maximum (1RM) at a constant load (no chains) or a variable load (30% 1RM constant load and 15% 1RM variable load; chains) with 7 days between conditions. For each repetition, the peak and mean velocity, peak power, peak acceleration, and time to peak velocity were recorded. Differences in peak and mean power were very likely trivial and unclear between the chain and no chain conditions, respectively. Possibly greater peak and likely greater mean bar velocity were accompanied by likely to most likely greater bar velocity between 50 and 400 ms from initiation of bench press in the chain condition compared with the no chain condition. Accordingly, bar acceleration was very likely greater in the chain condition compared with the no chain condition. In conclusion, these results show that the inclusion of chain resistance can acutely enhance several variables in the bench press throw and gives support to this type of training.

Feasibility of the 2-point method to determine the load-velocity relationship variables during the countermovement jump exercise.

PURPOSE: This study aimed to examine the reliability and validity of load-velocity (L-V) relationship variables obtained through the 2-point method using different load combinations and velocity variables. METHODS: Twenty men performed 2 identical sessions consisting of 2 countermovement jumps against 4 external loads (20 kg, 40 kg, 60 kg, and 80 kg) and a heavy squat against a load linked to a mean velocity (MV) of 0.55 m/s (load0.55). The L-V relationship variables (load-axis intercept (L0), velocity-axis intercept (v0), and area under the L-V relationship line (Aline)) were obtained using 3 velocity variables (MV, mean propulsive velocity (MPV), and peak velocity) by the multiple-point method including (20-40-60-80-load0.55) and excluding (20-40-60-80) the heavy squat, as well as from their respective 2-point methods (20-load0.55 and 20-80). RESULTS: The L-V relationship variables were obtained with an acceptable reliability (coefficient of variation (CV) ≤ 7.30%; intra-class correlation coefficient ≥ 0.63). The reliability of L0 and v0 was comparable for both methods (CVratio (calculated as higher value/lower value): 1.11-1.12), but the multiple-point method provided Aline with a greater reliability (CVratio = 1.26). The use of a heavy squat provided the L-V relationship variables with a comparable or higher reliability than the use of a heavy countermovement jump load (CVratio: 1.06-1.19). The peak velocity provided the load-velocity relationship variables with the greatest reliability (CVratio: 1.15-1.86) followed by the MV (CVratio: 1.07-1.18), and finally the MPV. The 2-point methods only revealed an acceptable validity for the MV and MPV (effect size ≤ 0.19; Pearson's product-moment correlation coefficient ≥ 0.96; Lin's concordance correlation coefficient ≥ 0.94). CONCLUSION: The 2-point method obtained from a heavy squat load and MV or MPV is a quick, safe, and reliable procedure to evaluate the lower-body maximal neuromuscular capacities through the L-V relationship.

Knowledge of results during vertical jump testing: an effective method to increase the performance but not the consistency of vertical jumps.

This study aimed to determine whether the provision of jump height feedback (knowledge of result; KR) can increase the performance and the consistency of output variables. In a randomised order, sixteen participants performed six squat or countermovement jumps (three from a 90º knee angle and three from a self-preferred knee angle) with or without KR over four sessions. The provision of KR significantly increased peak force (p = 0.046, 1.83%), mean force (p = 0.037, 1.45%), peak velocity (p < 0.001, 3.71%), mean velocity (p = 0.004, 3.44%), peak power (p < 0.001, 4.22%) and mean power (p = 0.001, 4.69%). A high within-session reliability was observed for all variables (coefficient of variation [CV] ≤ 5.62%, intraclass correlation coefficient [ICC] ≥ 0.95). No systematic differences in reliability were detected between the jumps performed without KR (CV = 3.00 ± 1.38%, ICC = 0.97 ± 0.03) and with KR (CV = 3.04 ± 1.49%, ICC = 0.97 ± 0.04). These results suggest that the provision of jump height feedback during vertical jump testing is effective to enhance vertical jump performance but it does not reduce the variability between jumps.

A low molecular weight proteome comparison of fertile and male sterile 8 anthers of Zea mays.

During maize anther development, somatic locular cells differentiate to support meiosis in the pollen mother cells. Meiosis is an important event during anther growth and is essential for plant fertility as pollen contains the haploid sperm. A subset of maize male sterile mutants exhibit meiotic failure, including ms8 (male sterile 8) in which meiocytes arrest as dyads and the locular somatic cells exhibit multiple defects. Systematic proteomic profiles were analysed in biological triplicates plus technical triplicates comparing ms8 anthers with fertile sibling samples at both the premeiotic and meiotic stages; proteins from 3.5 to 20 kDa were fractionated by 1-D PAGE, cleaved with Lys-C and then sequenced using a LTQ Orbitrap Velos MS paradigm. Three hundred and 59 proteins were identified with two or more assigned peptides in which each of those peptides were counted at least two or more times (0.4% peptide false discovery rate (FDR) and 0.2% protein FDR); 2761 proteins were identified with one or more assigned peptides (0.4% peptide FDR and 7.6% protein FDR). Stage-specific protein expression provides candidate stage markers for early anther development, and proteins specifically expressed in fertile compared to sterile anthers provide important clues about the regulation of meiosis. 49% of the proteins detected by this study are new to an independent whole anther proteome, and many small proteins missed by automated maize genome annotation were validated; these outcomes indicate the value of focusing on low molecular weight proteins. The roles of distinctive expressed proteins and methods for mass spectrometry of low molecular weight proteins are discussed.

Differential Repeated Sprinting Training in Youth Basketball Players: An Analysis of Effects According to Maturity Status.

The differential learning approach, which includes fluctuations that occur without movement repetitions and without corrections has received growing interest in the skill acquisition field. This study aimed to determine the effects of a 9-week training intervention involving differential repeated sprint training on a series of physical tests in youth basketball players. A total of 29 participants with different maturity statuses (pre-peak height velocity (PHV), n = 7; mid-PHV, n = 6; post-PHV, n = 16) completed 2 sessions per week of differential repeated sprint training for a period of 9 weeks. Sessions consisted of 2 × 10 repetitions sprints of 20-m whereby participants were instructed to perform various additional fluctuations for each repetition. Before and after the training intervention, participants completed jumping tests (countermovement jump (CMJ), single-leg CMJs, the modified 505 agility test, and straight sprinting tests (0-10 splits time), and maturity status was evaluated as well. Within-group analysis showed improvement in CMJ asymmetries and changes in direction asymmetries and 10-m sprint performance for the pre-, mid-, and post-PHV groups, respectively (p < 0.05), with large to very large effects. Analysis of covariance demonstrated that changes in sprint time in post-PHV players were greater than in the pre- and mid-PHV groups (p < 0.05), with moderate effect. Adding random fluctuations during repeated sprint training appear to be a suitable and feasible training strategy for maintaining and enhancing physical performance in youth basketball players, irrespective of maturity status. Furthermore, the present findings encourage practitioners to implement the present approach in youth athletes to improve their physical performance, but they should be aware that training response can vary according to maturity status.

Training Load, Maturity Timing and Future National Team Selection in National Youth Basketball Players.

Despite its importance to the management of training stress, monotony and recovery from exercise, training load has not been quantified during periods of intensity training in youths. This study aimed to (1) examine and quantify the training load (TL) in youth national team basketball players during a 2-week training camp according to maturity timing and (2) determine which parameters were related to under-18 (U18) national team selection. Twenty-nine U-16 national team basketball players underwent an anthropometric assessment to determine maturity timing. Players were categorised by maturity timing (early vs. average), whilst TL parameters during a 2-week training camp (i.e., 21 sessions) prior to FIBA U16 European Championship were used for group comparison and to predict future U-18 national team selection. The early-maturing players, who were taller and heavier (p < 0.05), experienced greater training strain in week 1 (p < 0.05) only. Irrespective of maturity timing, training loads in week 2 were predictive of onward selection for the U-18 national team. Conclusion: Based on present findings, practitioners are encouraged to develop their athletes' ability to tolerate high weekly loads, but also to be mindful that athletes' perceived exertion during national team training may be influenced by maturity timing.

The male sterile 8 mutation of maize disrupts the temporal progression of the transcriptome and results in the mis-regulation of metabolic functions.

Maize anther ontogeny is complex, with the expression of more than 30,000 genes over 4 days of cell proliferation, cell fate acquisition and the start of meiosis. Although many male-sterile mutants disrupt these key steps, few have been investigated in detail. The terminal phenotypes of Zea mays (maize) male sterile 8 (ms8) are small anthers exhibiting meiotic failure. Here, we document much earlier defects: ms8 epidermal cells are normal in number but fail to elongate, and there are fewer, larger tapetal cells that retain, rather than secrete, their contents. ms8 meiocytes separate early, have extra space between them, occupied by excess callose, and the meiotic dyads abort. Thousands of transcriptome changes occur in ms8, including ectopic activation of genes not expressed in fertile siblings, failure to express some genes, differential expression compared with fertile siblings and about 40% of the differentially expressed transcripts appear precociously. There is a high correlation between mRNA accumulation assessed by microarray hybridization and quantitative real-time reverse transcriptase polymerase chain reaction. Sixty-three differentially expressed proteins were identified after two-dimensional gel electrophoresis followed by liquid chromatography tandem mass spectroscopy, including those involved in metabolism, plasmodesmatal remodeling and cell division. The majority of these were not identified by differential RNA expression, demonstrating the importance of proteomics in defining developmental mutants.

Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize.

BACKGROUND: Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic changes in shielded leaves and immature ears. RESULTS: Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive. Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were altered by a 1 h UV-B treatment. CONCLUSIONS: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.

Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase I.

BACKGROUND: Developmental cues to start meiosis occur late in plants. Ameiotic1 (Am1) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. Pollen mother cells (PMCs) remain mitotic in most am1 mutants including am1-489, while am1-praI permits meiotic entry but PMCs arrest at the leptotene/zygotene (L/Z) transition, defining the roles of AM1 protein in two distinct steps of meiosis. To gain more insights into the roles of AM1 in the transcriptional pre-meiotic and meiotic programs, we report here an in depth analysis of gene expression alterations in carefully staged anthers at 1 mm (meiotic entry) and 1.5 mm (L/Z) caused by each of these am1 alleles. RESULTS: 1.0 mm and 1.5 mm anthers of am1-489 and am1-praI were profiled in comparison to fertile siblings on Agilent® 4 × 44 K microarrays. Both am1-489 and am1-praI anthers are cytologically normal at 1.0 mm and show moderate transcriptome alterations. At the 1.5-mm stage both mutants are aberrant cytologically, and show more drastic transcriptome changes. There are substantially more absolute On/Off and twice as many differentially expressed genes (sterile versus fertile) in am1-489 than in am1-praI. At 1.5 mm a total of 4,418 genes are up- or down-regulated in either am1-489 or am1-praI anthers. These are predominantly stage-specific transcripts. Many putative meiosis-related genes were found among them including a small subset of allele-specific, mis-regulated genes specific to the PMCs. Nearly 60% of transcriptome changes in the set of transcripts mis-regulated in both mutants (N = 530) are enriched in PMCs, and only 1% are enriched in the tapetal cell transcriptome. All array data reported herein will be deposited and accessible at MaizeGDB http://www.maizegdb.org/. CONCLUSIONS: Our analysis of anther transcriptome modulations by two distinct am1 alleles, am1-489 and am1-praI, redefines the role of AM1 as a modulator of expression of a subset of meiotic genes, important for meiotic progression and provided stage-specific insights into the genetic networks associated with meiotic entry and early prophase I progression.

Eccentric Resistance Training in Youth: A Survey of Perceptions and Current Practices by Strength and Conditioning Coaches.

BACKGROUND: Eccentric resistance training (ERT) in youth is advocated for aiding performance and injury risk. However, research investigating the applied practices of ERT in youth is in its infancy. In this study, we surveyed the perceptions and practices of practitioners utilizing ERT in youth to provide an understanding of its current application in practice. METHODS: Sixty-four strength and conditioning coaches completed an online survey reporting their current use of ERT in youth using both open and closed questions. RESULTS: Coaches deemed the inclusion of ERT important in youth with its inclusion based upon factors such as maturation status, training age and strength levels. Coaches also displayed an awareness of the physiological responses to eccentric exercise in youth compared to adults. ERT was primarily used for injury prevention, with the majority of coaches using body-weight and tempo exercises. Furthermore, utilizing eccentric hamstrings exercises was reported as highly important. The frequency of ERT tended to increase in older age groups and coaches mainly prescribed self-selected rest intervals. Finally, the need for further research into the training guidelines of ERT in youth was highlighted, in which coaches require more information on how maturation influences training adaptations and the fatigue-recovery responses. CONCLUSION: Coaches emphasized the importance of including ERT for both performance and injury prevention factors in youth although further research is required to generate practical guidelines for coaches in order to support its inclusion within practice.

Prediction of One Repetition Maximum Using Reference Minimum Velocity Threshold Values in Young and Middle-Aged Resistance-Trained Males.

BACKGROUND: This study determined the accuracy of different velocity-based methods when predicting one-repetition maximum (1RM) in young and middle-aged resistance-trained males. METHODS: Two days after maximal strength testing, 20 young (age 21.0 ± 1.6 years) and 20 middle-aged (age 42.6 ± 6.7 years) resistance-trained males completed three repetitions of bench press, back squat, and bent-over-row at loads corresponding to 20-80% 1RM. Using reference minimum velocity threshold (MVT) values, the 1RM was estimated from the load-velocity relationships through multiple (20, 30, 40, 50, 60, 70, and 80% 1RM), two-point (20 and 80% 1RM), high-load (60 and 80% 1RM) and low-load (20 and 40% 1RM) methods for each group. RESULTS: Despite most prediction methods demonstrating acceptable correlations (r = 0.55 to 0.96), the absolute errors for young and middle-aged groups were generally moderate to high for bench press (absolute errors = 8.2 to 14.2% and 8.6 to 20.4%, respectively) and bent-over-row (absolute error = 14.9 to 19.9% and 8.6 to 18.2%, respectively). For squats, the absolute errors were lower in the young group (5.7 to 13.4%) than the middle-aged group (13.2 to 17.0%) but still unacceptable. CONCLUSION: These findings suggest that reference MVTs cannot accurately predict the 1RM in these populations. Therefore, practitioners need to directly assess 1RM.

Mutator transposon activity reprograms the transcriptomes and proteomes of developing maize anthers.

Despite the high conservation of anther gene expression patterns across maize lines, Mu transposition programmed by transcriptionally active MuDR results in a 25% change in the transcriptome, monitored over 90 h of immature anther development, without altering the morphology, anatomy or pace of development. Most transcriptome changes are stage specific: cases of suppression of normal transcripts and ectopic activation are equally represented. Protein abundance changes were validated for numerous metabolic enzymes, and highlight the increased carbon and reactive oxygen management in Mutator anthers. Active Mutator lines appear to experience chronic stress, on a par with abiotic treatments that stimulate early flowering. Despite the diversity of acclimation responses, anther development progresses normally, in contrast to male-sterile mutants that disrupt anther cell fate or function completely, and cause fewer transcriptome changes. The early flowering phenotype ultimately confers an advantage in Mu element transmission.

Low Body Fat Does Not Influence Recovery after Muscle-Damaging Lower-Limb Plyometrics in Young Male Team Sport Athletes.

AIM: This study assessed the influence of fat mass to fat-free mass ratio (FM:FFM) on recovery from plyometric exercise. METHOD: After assessment of body composition, 20 male team sport players (age 20.7 ± 1.1 years; body mass 77.1 ± 11.5 kg) were divided into low- (n = 10; 0.11 ± 0.03) and normal- (n = 10; 0.27 ± 0.09) fat groups based on FM:FFM ratio. Thereafter, participants completed measurements of knee extensor torque at 60 and 240°âˆ™s-1, countermovement jump flight time, plasma creatine kinase (CK) activity and perceived muscle soreness (VAS) before and at 0, 24 and 48 h after 10 × 10 maximal plyometric vertical jumps. RESULTS: Evidence of muscle damage was confirmed by alterations in VAS, peak torque at 60 and 240°âˆ™s-1 and flight time at 0, 24 and 48 h after plyometric exercise (P < 0.05). CK was increased at 0 and 24 h (P < 0.05) but returned to baseline values by 48 h. No time by group effects were observed for any of the dependent variables (P > 0.05). CONCLUSION: The current findings indicate that while muscle damage was present after plyometric exercise, the magnitude was similar across the two body composition groups. Applied practitioners can allow for a similar recovery time after plyometric exercise in those with low and normal body fat.